LOCUS Exported 9890 bp ds-DNA circular SYN 27-JAN-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS PPTLUC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9890) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Jan 27, 2017 from SnapGene Viewer 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9890 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 234..1886 /codon_start=1 /gene="luc+" /product="firefly luciferase" /note="luciferase" /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" polyA_signal 1927..2048 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" misc_feature 2161..2393 /note="P element 3' end" promoter 3195..3299 /gene="bla" /note="AmpR promoter" CDS 3300..4160 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 4331..4919 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 5154..5739 /note="P element 5' end" CDS complement(join(6517..7131,7202..7333,7554..7869,7931..8585, 8660..8933,9328..9399)) /codon_start=1 /gene="white" /product="Drosophila white gene eye color pigment" /note="mini-white" /note="This is a modified version of the white gene lacking part of the first intron." /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE" ORIGIN 1 aggccttcta gaccgcggcc gcagatctgt taacgaattc ggtaccagat ccgggatccg 61 gcgaaaagag cgccggagta taaatagagg cgcttcgtct acggagcgac aattcaattc 121 aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga 181 acaagctaaa caatctgcaa gcttggcatt ccggtactgt tggtaaagcc accatggaag 241 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg 301 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta 361 cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt 421 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg 481 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg 541 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc 601 ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc 661 tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt 721 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc 781 cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg 841 gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca 901 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat 961 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg 1021 tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc 1081 aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca 1141 aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag 1201 tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca 1261 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg 1321 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg 1381 gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa 1441 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca 1501 tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta 1561 agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca 1621 acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg 1681 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg 1741 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac 1801 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca 1861 agaagggcgg aaagatcgcc gtgtaattct agagtcgggg cggccggccg cttcgagcag 1921 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat 1981 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata 2041 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg 2101 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gcccaagctt 2161 tgcgtactcg caaattatta aaaataaaac tttaaaaata atttcgtcta attaatatta 2221 tgagttaatt caaaccccac ggacatgcta agggttaatc aacaatcata tcgctgtctc 2281 actcagactc aatacgacac tcagaatact attcctttca ctcgcactta ttgcaagcat 2341 acgttaagtg gatgtctctt gccgacggga ccaccttatg ttatttcatc atggtctggc 2401 cattctcatc gtgagcttcc gggtgctcgc atatctggct ctaagacttc gggcccgacg 2461 caaggagtag ccgacatata tccgaaataa ctgcttgttt ttttttttac cattattacc 2521 atcgtgttta ctgtttattg ccccctcaaa aagctaatgt aattatattt gtgccaataa 2581 aaacaagata tgacctatag aatacaagta tttccccttc gaacatcccc acaagtagac 2641 tttggatttg tcttctaacc aaaagactta cacacctgca taccttacat caaaaactcg 2701 tttatcgcta cataaaacac cgggatatat tttttatata catacttttc aaatcgcgcg 2761 ccctcttcat aattcacctc caccacacca cgtttcgtag ttgctctttc gctgtctccc 2821 acccgctctc cgcaacacat tcaccttttg ttcgacgacc ttggagcgac tgtcgttagt 2881 tccgcgcgat tcggtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct 2941 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac 3001 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 3061 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac 3121 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt 3181 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 3241 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 3301 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 3361 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 3421 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 3481 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 3541 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 3601 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 3661 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 3721 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 3781 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 3841 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 3901 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 3961 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 4021 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 4081 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 4141 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 4201 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 4261 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 4321 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 4381 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 4441 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 4501 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 4561 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 4621 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 4681 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc 4741 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 4801 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 4861 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 4921 acgccttctt cttgaactcg ggctcggtgc cagtatacct caaatggttg tcgtacctct 4981 catggttccg ttacgccaac gagggtctgc tgattaacca atgggcggac gtggagccgg 5041 gcgaaattag ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg 5101 agacgcttaa cttctccgcc gccgatctgc cgctggacta cgtgggtctg gcccatgatg 5161 aaataacata aggtggtccc gtcgatagcc gaagcttacc gaagtataca cttaaattca 5221 gtgcacgttt gcttgttgag aggaaaggtt gtgtgcggac gaattttttt ttgaaaacat 5281 taacccttac gtggaataaa aaaaaatgaa atattgcaaa ttttgctgca aagctgtgac 5341 tggagtaaaa ttaattcacg tgccgaagtg tgctattaag agaaaattgt gggagcagag 5401 ccttgggtgc agccttggtg aaaactccca aatttgtgat acccacttta aagattcgca 5461 gtggaaggct gcacctgcaa aaggtcagac atttaaaagg aggcgactca acgcagatgc 5521 cgtacctagt aaagtgatag agcctgaacc agaaaagata aaagaaggct ataccagtgg 5581 gagtacacaa acagagtaag tttgaatagt aaaaaaaatc atttatgtaa acaataacgt 5641 gactgtgcgt taggtcctgt tcattgttta atgaaaataa gagcttgagg gaaaaaattc 5701 gtactttgga gtacgaaatg cgtcgtttag agcagcagcc gaattaattc tagttccagt 5761 gaaatccaag cattttctaa attaaatgta ttcttattat tatagttgtt atttttgata 5821 tatataaaca acactattat gcccaccatt tttttgagat gcatctacac aaggaacaaa 5881 cactggatgt cactttcagt tcaaattgta acgctaatca ctccgaacag gtcacaaaaa 5941 attaccttaa aaagtcataa tattaaatta gaataaatat agctgtgagg gaaatatata 6001 caaatatatt ggagcaaata aattgtacat acaaatattt attactaatt tctattgaga 6061 cgaaatgaac cactcggaac catttgagcg aaccgaatcg cgcggaacta acgacagtcg 6121 ctccaaggtc gtcgaacaaa aggtgaatgt gttgcggaga gcgggtggga gacagcgaaa 6181 gagcaactac gaaacgtggt gtggtggagg tgaattatga agagggcgcg cgatttgaaa 6241 agtatgtata taaaaaatat atcccggtgt tttatgtagc gataaacgag tttttgatgt 6301 aaggtatgca ggtgtgtaag tcttttggtt agaagacaaa tccaaagtct acttgtgggg 6361 atgttcgaag gggaaatact tgtattctat aggtcatatc ttgtttttat tggcacaaat 6421 ataattacat tagctttttg agggggcaat aaacagtaaa cacgatggta ataatggtaa 6481 aaaaaaaaac aagcagttat ttcggatata tgtcggctac tccttgcgtc gggcccgaag 6541 tcttagagcc agatatgcga gcacccggaa gctcacgatg agaatggcca gacccacgta 6601 gtccagcggc agatcggcgg cggagaagtt aagcgtctcc aggatgacct tgcccgaact 6661 ggggcacgtg gtgttcgacg atgtgcagct aatttcgccc ggctccacgt ccgcccattg 6721 gttaatcagc agaccctcgt tggcgtaacg gaaccatgag aggtacgaca accatttgag 6781 gtatactggc accgagcccg agttcaagaa gaagccgcca aagagcagga atggtatgat 6841 aaccggcgga cccacagaca gcgccatcga ggtcgaggag ctggcgcagg atattagata 6901 tccgaaggac gttgacacat tggccaccag agtgaccagc gccaggcagt tgaagaagtg 6961 cagcactccg gcccgcagtc cgatcatcgg ataggcaatc gccgtgaaga ccagtggcac 7021 tgtgagaaaa agcggtaatt cggcaatcgt tttgcccaga aagtatgtgt cacagcgata 7081 aagtcgactt cgggcctccc tcataaaaac tggcagctct gaggtgaaca cctaaatcga 7141 atcgattcat tagaaagtta gtaaattatt aatatgcaaa tgtattctaa acaagactta 7201 catttatcgt ggcaaagacg ttttgaaagg tcatgttggt caggaagagg aagatggctc 7261 cgttgatatt catcacgccc acttgcgtga gttgttggcc caaaaagatg aggccaatca 7321 agatggcaac catctgcaaa ttaaaatgtt actcgcatct cattaatatt catatcttca 7381 acatgttcgc gagttaaatg aaatttattt attttctgca aaactataaa ctatacatct 7441 cattgaaaaa aactaagaag ggtgtggaat caggcaattc taactaaaat ctagcgaatt 7501 tgtttccaag aattgtaagc gttatatcat ttgtttccac tggaaccact caccgttgtc 7561 tgaataagtc gcacttttac gaggagtggt tccttgagca ccgacagcca ggatcgccac 7621 aggaccgccc ggaactgcat gaaccaggtg gccttgtagg tgtacccatt ctccggctgc 7681 tccagtggct tctccaaatt tttggtggcc aacaactgct ccatatcccg ggctactttg 7741 ctaatagcaa aattgtcgca tatcttggcg atccgatcac gggactcgat ctcccgtccg 7801 ggcacaacgg ccaacacctg tacgtaaaag tccgccggat tgtagttggt aggacactgg 7861 gcacccacgc tggataggag ttgagatgta atgtaatgct agataccctt aataaacaca 7921 tcgaactcac taggaaaaga agtcgacggc ttcgctggga gtgcccaaga aagctaccct 7981 gccctcggcc atcagaagga tcttgtcaaa gagctcaaac agctcggaag acggctgatg 8041 aatggtcagg atgacggtct tgcccttctg cgacagcttc ttcagcacct ggacgacgct 8101 gtgggcggta aatgagtcca gtccggaggt gggctcatcg cagatcagaa gcggcggatc 8161 ggttagtgcc tcggaggcga atgccagacg cttcctttct ccgccggaca gacctttcac 8221 cctgccgggc acaccgatga tcgtgtgctg acatttgctg agcgaaagct cctggatcac 8281 ctgatccacg cgggccactc gctgccgata ggtcagatgt cgtggcatcc gcaccatggc 8341 ctggaaaatc aggtgttccc tggccgttag ggagccgata aagaggtcat cctgctggac 8401 ataggcgcac ctggcctgca tctccttggc gtccacaggt tggccattga gcagtcgcat 8461 cccggatggc gatacttgga tgccctgcgg cgatcgaaag gcaagggcat tcagcagggt 8521 cgtctttccg gcaccggaac tgcccatcac ggccaaaagt tcgcccggat aggccacgcc 8581 gcaaactgag tttcaaattg gtaattggac cctttattaa gatttcacac agatcagccg 8641 actgcgaata gaaactcacc gttcttgagc aaatgtttcc tgggcgccgg tatgtgtcgc 8701 tcgttgcaga atagtccgcg tgtccggttg accagctgcc gccatccgga gcccggctga 8761 ttgaccgccc caaagatgtc catattgtgc caggcatagg tgaggttctc ggctagttgg 8821 ccgctccctg aaccggagtc ctccggcgga ctgggtggca ggagcgtgcc gtagtttttg 8881 gcctgcccga agccctggtt aatgcagctc tgcgaagccg ctccgctgtc accctgcaat 8941 gataggggat ctcaaatatc aactacaagc gttatgctca tctaaccccg aacaaaacga 9001 agtatcctac gaagtaggtt tatactttta tttatttttt gtgcatctag gatcagctta 9061 aaatatctgg ttgttatatt ttttgtaaaa aagaatgtag tcgaaaatga atgcctttag 9121 atgtcttgat catgatatga tcttaaaaat tgtcttatat agcgagcaca gctaccagaa 9181 taatctgttt cgtgtcacta tttgtttgtg cgattgcggt ttgggatttt tgtgggtcgc 9241 agttctcacg ccgcagacaa tttgatgttg caatcgcagt tcctatagat caagtgaact 9301 taagatgtat gcacatgtac tactcacatt gttcagatgc tcggcagatg ggtgtttgct 9361 gcctccgcga attaatagct cctgatcctc ttggcccatt gccgggattt ttcacacttt 9421 cccctgctta cccacccaaa accaatcacc accccaatca ctcaaaaaac aaacaaaaat 9481 aagaagcgag aggagttttg gcacagcact ttgtgtttaa ttgatggcgt aaaccgcttg 9541 gagcttcgtc acgaaaccgc tgacaaagtg caactgaagg cggacattga cgctaggtaa 9601 cgctacaaac ggtggcgaaa gagatagcgg acgcagcggc gaaagagacg gcgatatttc 9661 tgtggacaga gaaggaggca aacagcgctg actttgagtg gaatgtcatt ttgagtgaga 9721 ggtaatcgaa agaacctggt acttcaaata cccttggatc gaagtaaatt taaaactgat 9781 cagataagtt caatgatatc cagtgcagta aaaaataaaa aaaaaatatg tttttttaaa 9841 tctacattct ccaaaaaagg gttttattaa cttacataca tactagaatt //