LOCUS pCDNA5_MTS_TagBF 7650 bp ds-DNA linear 01-FEB-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCDNA5-MTS-TagBFP-PT-EGFP-NLS-PT-mCherry-PTS1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7650) AUTHORS . TITLE Direct Submission JOURNAL Exported 2017年2月1日 from SnapGene 2.8.3 http://www.snapgene.com COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers enhancer 235..614 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /label=CMV enhancer /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 615..818 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" /label=CMV promoter /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 protein_bind 820..838 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" /label=tetO /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 protein_bind 841..859 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" /label=tetO(1) /ApEinfo_label=tetO /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 976..1062 /note="Cytochrome c oxidase subunit 8A MTS 29 aa" /label=Cytochrome c oxidase subunit 8A MTS 29 aa /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1084..1782 /codon_start=1 /product="monomeric blue fluorescent protein" /note="TagBFP" /note="mammalian codon-optimized" /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK V VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT A TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL K LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY C DLPSKLGHKLN" /label=monomeric blue fluorescent protein /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1822..1878 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /note="P2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" /label=2A peptide from porcine teschovirus-1 polyprotein /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1888..1941 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /note="T2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" /label=2A peptide from Thosea asigna virus capsid protei /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1960..2676 /codon_start=1 /product="enhanced GFP" /note="EGFP" /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT L KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD D GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGI K VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVL L EFVTAAGITLGMDELYK" /label=enhanced GFP /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 2683..2703 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /translation="PKKKRKV" /label=nuclear localization signal of SV40 large T antig /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 2728..2784 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /note="P2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" /label=2A peptide from porcine teschovirus-1 polyprotein(1) /ApEinfo_label=2A peptide from porcine teschovirus-1 polyprotein /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 2794..2847 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /note="T2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" /label=2A peptide from Thosea asigna virus capsid protei(1) /ApEinfo_label=2A peptide from Thosea asigna virus capsid protei /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 2848..3555 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /note="mCherry" /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE G TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMN F EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGAL K GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYER A EGRHSTGGMDELYK" /label=monomeric derivative of DsRed fluorescent protein /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3565..3579 /note="PTS1" /label=PTS1 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 polyA_signal 3608..3832 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" /label=bGH poly(A) signal /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 protein_bind 4116..4163 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." /label=FRT /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 4171..5193 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /note="HygR" /note="confers resistance to hygromycin" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG Y VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP E TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ T VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF G DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG N FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E " /label=aminoglycoside phosphotransferase from E. coli /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 polyA_signal 5323..5444 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /label=SV40 poly(A) signal /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(5493..5509) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" /label=M13 rev /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 protein_bind 5517..5533 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /label=lac operator /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter complement(5541..5571) /note="lac promoter" /note="promoter for the E. coli lac operon" /label=lac promoter /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 protein_bind 5586..5607 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." /label=CAP binding site /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(5895..6483) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label=ori /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(6654..7514) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY I ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY S PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR W EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS A LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA S LIKHW" /label=beta-lactamase /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter complement(7515..7619) /gene="bla" /note="AmpR promoter" /label=bla /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 961 ctctagcgtt taaacatgtc cgtcctgacg ccgctgctgc tgcggggctt gacaggctcg 1021 gcccggcggc tcccagtgcc gcgcgccaag atccattcgt tggggggtcc caccggtgcc 1081 accatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg 1141 gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag 1201 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct 1261 actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc 1321 ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg 1381 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc 1441 aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc 1501 tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac 1561 atggccctga agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga 1621 tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg 1681 gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc 1741 agatactgcg acctccctag caaactgggg cacaagctga attccggagg cagaaagctt 1801 ggttccggta ccggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg 1861 gaggagaacc ctggacctgg aagcggagag ggcagaggaa gtctgctaac atgcggtgac 1921 gtcgaggaga atcctggacc taccgagctc ggatctacca tggtgagcaa gggcgaggag 1981 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 2041 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 2101 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac 2161 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 2221 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 2281 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 2341 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac 2401 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag 2461 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 2521 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc 2581 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 2641 gccgggatca ctctcggcat ggacgagctg tacaagggat cccctaagaa gaagcgcaag 2701 gtcctcgaga gcggccgcgg aagcggagct actaacttca gcctgctgaa gcaggctgga 2761 gacgtggagg agaaccctgg acctggaagc ggagagggca gaggaagtct gctaacatgc 2821 ggtgacgtcg aggagaatcc tggacctatg gtgagcaagg gcgaggagga taacatggcc 2881 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag 2941 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 3001 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg 3061 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc 3121 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc 3181 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc 3241 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 3301 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag 3361 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc 3421 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag 3481 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg 3541 gacgagctgt acaaggctag cggatacaag agtaagctgt aataacccgg gtaatgatca 3601 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 3661 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 3721 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 3781 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 3841 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 3901 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 3961 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 4021 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 4081 aaaaaacttg attagggtga tggttcacgt acctagaagt tcctattccg aagttcctat 4141 tctctagaaa gtataggaac ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc 4201 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc 4261 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat 4321 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg 4381 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc 4441 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt 4501 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc 4561 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata 4621 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt 4681 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc 4741 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata 4801 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac 4861 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg 4921 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt 4981 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt 5041 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc 5101 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga 5161 cgccccagca ctcgtccgag ggcaaaggaa tagcacgtac tacgagattt cgattccacc 5221 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 5281 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct 5341 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 5401 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg 5461 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 5521 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 5581 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 5641 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 5701 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 5761 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 5821 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 5881 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 5941 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 6001 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 6061 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 6121 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 6181 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 6241 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 6301 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 6361 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 6421 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 6481 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 6541 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 6601 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 6661 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 6721 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 6781 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 6841 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 6901 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 6961 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 7021 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 7081 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 7141 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 7201 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 7261 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 7321 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 7381 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 7441 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 7501 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 7561 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 7621 acatttcccc gaaaagtgcc acctgacgtc //