LOCUS       pCAG\VSFP2.3\rev        7979 bp    DNA     circular      5-FEB-2010
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDATE|545163639|
COMMENT     VNTDBDATE|545164983|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCAG VSFP2.3 rev with Flex|
COMMENT     VNTAUTHORNAME|Yuka Iwamoto|
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FEATURES             Location/Qualifiers
     CDS             complement(1934..4108)
                     /vntifkey="4"
                     /label=VSFP2.3
     misc_signal     1789..1822
                     /vntifkey="87"
                     /label=lox2272
     misc_signal     4131..4164
                     /vntifkey="87"
                     /label=lox2272
     misc_marker     1885..1918
                     /vntifkey="22"
                     /label=loxP
     misc_marker     4235..4268
                     /vntifkey="22"
                     /label=loxP
     LTR             4312..4899
                     /vntifkey="19"
                     /label=WPRE
     promoter        1731..1734
                     /vntifkey="29"
                     /label=CAGGS\Pro.
     primer          4715..4735
                     /vntifkey="27"
                     /label=Fwd-11
     primer_bind     4415..4433
                     /vntifkey="28"
                     /label=AAV-CAGSeq-Rev
     primer          4209..4230
                     /vntifkey="27"
                     /label=Fwd-25
     promoter        18..1677
                     /vntifkey="29"
                     /label=CAG\promoter
     rep_origin      5651..5987
                     /vntifkey="33"
                     /label=SV40_ORI
     misc_marker     complement(6989..7849)
                     /vntifkey="22"
                     /label=Amp\(R)
     rep_origin      6198..6832
                     /vntifkey="33"
                     /label=COLE1_ORI
BASE COUNT     1614 a      2160 c      2180 g      2025 t 
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc 
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt 
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg 
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgctagc ctgggcaacg 
     1741 tgctggttat tgtgctgtct catcattttg gcaaagaatt ggatccccat aacttcgtat 
     1801 aaagtatcct atacgaagtt atatcaaaat aggaagacca atgcttcacc atcgacccga 
     1861 attgccaagc atcaccatcg acccataact tcgtataatg tatgctatac gaagttatac 
     1921 taggatcccc ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga 
     1981 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggt 
     2041 agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct 
     2101 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca 
     2161 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt 
     2221 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga 
     2281 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt 
     2341 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga 
     2401 agtcgtgctg cttcatgtgg tcggggtagc gcgcgaagca catcaggccg tagccgaagg 
     2461 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg 
     2521 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt 
     2581 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct 
     2641 tgctcaccat gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc 
     2701 gcttctcgtt ggggtctttg ctcagcttgg actgggtgct caggtagtgg ttgtcgggca 
     2761 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc 
     2821 tgccgtcctc gatgttgtgg cggatcttga agttggcctt gatgccgttc ttctgcttgt 
     2881 cggcggtgat atagacgttg tcgctgatgg cgttgtactc cagcttgtgc cccaggatgt 
     2941 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct 
     3001 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct 
     3061 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg 
     3121 ggtagcgggc gaagcactgc acgccccagg tcagggtggt cacgagggtg ggccagggca 
     3181 cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc 
     3241 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca 
     3301 ggatgggcac caccccggtg aacagctcct cgcccttgct caccattcga cgcttgttct 
     3361 gtgatattgt tcttctgctt gaagccttca tttgttggtg ggaataaaat attcttgcta 
     3421 atcgaaccac acgcagcaaa cgggccaaca caaccaactg ccccaaacca tcggctcctg 
     3481 tttcttgcac gtattcatct aacacagtgt aaaatatcgt gacgacgaat gtgacaacga 
     3541 taatcaaacc atcagcaacc tcccaggggt tggtgaagaa attcttgggc ccgtaggcaa 
     3601 atatccttaa tcctaaatcc agcatgaaat aacaagaaag agccaaagcc atcccgtcat 
     3661 aaaaggattg tgaagattca ctttttcctg gaagactgag atcaatgatc atgaggatga 
     3721 tgtccaagaa aattaggaag actccaaaga ctcgcatccc tagatgatca atcactgctc 
     3781 ggacacgaaa ctggacgcga cctacaccag tagtaggtat ctccattctc ccatcgtcca 
     3841 ctccatgttc attctcgccc caccatagac ctgaaatctc gggtatatct attcgttcct 
     3901 cctgtcttgt ctctggtttt tcactcggtc cttggtctac atcattccaa tgaactttct 
     3961 ttaccgattc actttttctt ggcgcagctt tcggcggagc agtgacgtca ccatttatcg 
     4021 tgacgtcaac gacgttccgc atgactgcac cgtcaacgcc aactaaatca gctggaggac 
     4081 taaaatctga accgtcgaat ccctccatgg tggcggtacc gaattctagt ataacttcgt 
     4141 ataggatact ttatacgaag ttatcattgg gattcttcct attttgatcc aagcatcacc 
     4201 atcgaccctc tagtccagat ctcaccatcg acccataact tcgtatagca tacattatac 
     4261 gaagttatgt ccctcgaaga ggttcgaatt cgatatcaag cttatcgata atcaacctct 
     4321 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 
     4381 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 
     4441 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 
     4501 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 
     4561 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 
     4621 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 
     4681 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc 
     4741 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 
     4801 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 
     4861 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gcttaaggcg 
     4921 gccgcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc 
     4981 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga 
     5041 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt 
     5101 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac 
     5161 atcagaatga gtatttggtt tagagtttgg caacatatgc catatgctgg ctgccatgaa 
     5221 caaaggtggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta 
     5281 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt 
     5341 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc 
     5401 tcctgactac tcccagtcat agctgtccct cttctcttat gaagatccct cgacctgcag 
     5461 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     5521 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     5581 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     5641 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 
     5701 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 
     5761 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 
     5821 ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg 
     5881 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 
     5941 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta 
     6001 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 
     6061 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 
     6121 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 
     6181 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 
     6241 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 
     6301 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 
     6361 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 
     6421 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 
     6481 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 
     6541 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 
     6601 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 
     6661 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 
     6721 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 
     6781 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 
     6841 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 
     6901 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 
     6961 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 
     7021 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 
     7081 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 
     7141 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 
     7201 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 
     7261 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 
     7321 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 
     7381 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 
     7441 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 
     7501 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 
     7561 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 
     7621 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 
     7681 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 
     7741 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 
     7801 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 
     7861 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 
     7921 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctg 
//
