LOCUS       5547_bp                 5547 bp ds-DNA     circular     13-FEB-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             3720..4580
                     /label="ampicillin_resistance_gene_ORF Selectable marker|CDS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      3283..3440
                     /label="rrnB_transcriptional_termination_region Terminator|terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    290..1249
                     /label="Homologous Regions Fw"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        3650..3678
                     /label="ampicillin_resistance_gene(b-lactamase)promoter Promoter|promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1368..2027
                     /label="chloramphenicol_acetyltransferase(CAT)_ORF Selectable marker|CDS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      4735..5354
                     /label="pBR322_replication_origin Replication origin|rep_origin"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2118..3098
                     /label="Homologous Region Bck"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
      121 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
      181 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
      241 accgaaacgc gcgaggcagc agatcaattc gcgcgcgaag gcgaagcggg cgattaaata
      301 aggaggaata aaccatgttg gaacgggagc agaattttac ccctgtcacc accagtgtgg
      361 tcgatcgcca agtgttatcg aggggttccc aggaaaaagt ggtggacaac attgtccgca
      421 aagatgggga agaaacgccg gatttaattg tgctgacccc cacctgtacc tccagcattt
      481 tgcaggaaga tttggccaat tttgttgacc gggcccagat ggatgcccac tgtgatgtac
      541 ttttggcgga cgtgaaccat taccgctata acgaattgca agctggcgat cgtaccctta
      601 agcagattgt cgagttttac atcaaaaaag cccggaaaca gggtaattta gccacggaaa
      661 aaacagctaa gccatcggtg aatttaattg gcttcaccac cctaggtttc cacaatcaac
      721 atgattgcac cgagttaaag cgattaatgg cggatctggg cattgaagta aacctgattt
      781 tgccggaaaa agccacggtg gatcaattgg ctaaaattcc ccaagcttgg tttaacctct
      841 gcccgtaccg ggaaattggt ttgatgacag cggaatattt gcaagaagaa tttgcccagc
      901 cctacgtgga cattaccccc atgggagtgg tggaaacggc ccgttgtatc cgcaaaatgc
      961 aacaggtgtt aaatcaacag ggtttcgacg ttaactacga agatttcatc cagcaacaaa
     1021 cccgtcacgt ctcccaagcg gcctggtttt ctcgctctat cgactgtcaa aatctcacgg
     1081 gcaaaaaagc agtggtcttt ggggataata cccatgcggc cgctatgacc aaaattttgg
     1141 cccgggaaat gggtatccac gttgtgctag caggaaccta ttgcaaatac gatgccgatt
     1201 ggttccgagc cgaagtatct gaatactgtg acgaagtgtt gatcagtgaa gacaacgggg
     1261 ccgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg
     1321 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
     1381 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     1441 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     1501 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     1561 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
     1621 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     1681 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     1741 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     1801 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     1861 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     1921 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     1981 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaagggc
     2041 atgctgagct cgagatctgc agctggtacc atatgggaat tcaacgcgtc cagttctttc
     2101 aaaaacgtcc acgccattat gcacgctccg ttgggggatg attatttcaa cgtcatgcgc
     2161 tccatgttgg aacgggagca gaattttacc cctgtcacca ccagtgtggt cgatcgccaa
     2221 gtgttatcga ggggttccca ggaaaaagtg gtggacaaca ttgtccgcaa agatggggaa
     2281 gaaacgccgg atttaattgt gctgaccccc acctgtacct ccagcatttt gcaggaagat
     2341 ttggccaatt ttgttgaccg ggcccagatg gatgcccact gtgatgtact tttggcggac
     2401 gtgaaccatt accgctataa cgaattgcaa gctggcgatc gtacccttaa gcagattgtc
     2461 gagttttaca tcaaaaaagc ccggaaacag ggtaatttag ccacggaaaa aacagctaag
     2521 ccatcggtga atttaattgg cttcaccacc ctaggtttcc acaatcaaca tgattgcacc
     2581 gagttaaagc gattaatggc ggatctgggc attgaagtaa acctgatttt gccggaaaaa
     2641 gccacggtgg atcaattggc taaaattccc caagcttggt ttaacctctg cccgtaccgg
     2701 gaaattggtt tgatgacagc ggaatatttg caagaagaat ttgcccagcc ctacgtggac
     2761 attaccccca tgggagtggt ggaaacggcc cgttgtatcc gcaaaatgca acaggtgtta
     2821 aatcaacagg gtttcgacgt taactacgaa gatttcatcc agcaacaaac ccgtcacgtc
     2881 tcccaagcgg cctggttttc tcgctctatc gactgtcaaa atctcacggg caaaaaagca
     2941 gtggtctttg gggataatac ccatgcggcc gctatgacca aaattttggc ccgggaaatg
     3001 ggtatccacg ttgtgctagc aggaacctat tgcaaatacg atgccgattg gttccgagcc
     3061 gaagtatctg aatactgtga cgaagtgttg atcagtgaag acaacggggc tctagaacaa
     3121 aaactcatct cagaagagga tctgaatagc gccgtcgacc atcatcatca tcatcattga
     3181 gtttaaacgg tctccagctt ggctgttttg gcggatgaga gaagattttc agcctgatac
     3241 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
     3301 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
     3361 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     3421 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     3481 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     3541 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
     3601 ggcctttttg cgtttctaca aactcttttt gtttattttt ctaaatacat tcaaatatgt
     3661 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3721 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3781 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3841 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3901 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3961 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4021 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4081 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4141 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4201 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4261 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4321 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4381 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4441 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4501 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4561 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4621 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4681 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4741 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4801 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4861 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4921 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4981 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5041 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5101 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5161 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5221 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5281 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5341 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5401 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5461 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5521 agcgcctgat gcggtatttt ctcctta
//