LOCUS       480_N-BLA_1.1(pToxRVII_N-BLA(PelB_AN)M180T-sfGFP)_p15a_corrected      5607 bp    DNA     circular UNA 
DEFINITION  araBAD-Promoter anti aIIb
FEATURES             Location/Qualifiers
     Vector          join(1857..5607,1..141)
                     /origin="pToxRVII (5.952bp circular)Target vector: from
                     XhoI cut at 2201^2202 to MluI cut at 141^142,
                     3.892bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     Region          10..25
                     /note="Match: AAACCAATTGTCCATA"
                     /note="Length of match: 16"
                     /note="Accuracy (%): 100"
                     /note="Description: 2639_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_O2_operator
     Primer_binding_ 103..122
                     /note="Primer: GCATTCTGTAACAAAGCGGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 103..122"
                     /label="57pToxRV_fwd(ToxSeqUp) (mismatches: 0)"
     Insertion       142..1856
                     /origin="pToxRV_N-BLA(PelB)M180T-sfGFP (5.505bp
                     circular)Fragment: from MluI cut at 4744^4745 to XhoI cut
                     at 930^931, 1.691bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label=Insert
     Region          167..178
                     /note="Match: CGGCAGAAAAGT"
                     /note="Length of match: 12"
                     /note="Accuracy (%): 100"
                     /note="Description: 1540_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_O1_operator
     Promoter        194..203
                     /label="araBAD promoter region, cAMP-RP binding residues, (Nennenga 2011)"
     Region          209..222
                     /note="Match: TTGCTATGCCATAG"
                     /note="Length of match: 14"
                     /note="Accuracy (%): 100"
                     /note="Description: 1D6E_checksum."
                     /note="Found at strand: Positive"
                     /label=CAP_binding_site
     Primer_binding_ 214..233
                     /note="Primer: ATGCCATAGCATTTTTATCC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 214..233"
                     /label="primer_GATC-pBAD-FP (mismatches: 0)"
     Region          254..282
                     /note="Match: CTGACGCTTTTTATCGCAACTCTCTACTG"
                     /note="Length of match: 29"
                     /note="Accuracy (%): 100"
                     /note="Description: 7B4_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_promoter
     Primer_binding_ 304..331
                     /note="Primer: GGGCTCTAGATAACGAGGGCAAAAAATG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 304..331"
                     /label="101MT028 (mismatches: 0)"
     Primer_binding_ complement(304..361)
                     /note="Primer:
                     tcCaGCGGCtGCcgtagGCAaTaggTAtTtCATTTTTTGCCCTCGTTATCTAGAGCCC
                     "
                     /note="Number of mismatches: 15"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(304..361)"
                     /label="137pelBrev (mismatches: 15)"
     Primer_binding_ complement(304..347)
                     /note="Primer:
                     gatGgtGgTgATgATGCATTTTTTGCCCTCGTTATCTAGAGCCC"
                     /note="Number of mismatches: 8"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(304..347)"
                     /label="79MT023 (mismatches: 8)"
     Primer_binding_ complement(307..361)
                     /note="Primer:
                     taaacCaGCCagcgcCAGCcaaaTcTttTtCATTTTTTGCCCTCGTTATCTAGAG"
                     /note="Number of mismatches: 19"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(307..361)"
                     /label="292DsbASPrev (mismatches: 19)"
     Primer_binding_ complement(309..400)
                     /note="Primer:
                     gtgagCaaaaacAGgaaggcaaaatgcCGCaAaaaagGgaataagGGCgaCAcggAaa
                     tGTTgAatActCATTTTTTGCCCTCGTTATCTAG"
                     /note="Number of mismatches: 50"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(309..400)"
                     /label="438_SP-BLA_rev (mismatches: 50)"
     Primer_binding_ complement(309..394)
                     /note="Primer:
                     cGccgcctgcgcCACgGtcgcaaagcccgcCagcGCCaCcGCaatcgcaAtCgcggTc
                     TttTtCATTTTTTGCCCTCGTTATCTAG"
                     /note="Number of mismatches: 46"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(309..394)"
                     /label="439_OmpA_rev (mismatches: 46)"
     Primer_binding_ complement(309..397)
                     /note="Primer:
                     gTggGccatcGCcggCtggGCagcgaggAGcagCagacCaGCaGCaGCggtCgGCAGc
                     aggTAtTtCATTTTTTGCCCTCGTTATCTAG"
                     /note="Number of mismatches: 41"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(309..397)"
                     /label="437_PelB-v_rev (mismatches: 41)"
     Primer_binding_ complement(309..331)
                     /note="Primer: CATTTTTTGCCCTCGTTATCTAG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(309..331)"
                     /label="299BLA_delta_SP_rev (mismatches: 0)"
     Primer_binding_ 312..402
                     /note="Primer:
                     GATAACGAGGGCAAAAAATGCATTATAAACTGCTGTTTGCGGCCGCGGCCGCGAGCCT
                     GGCGAGCGCGGTGAGCGCTCACCCAGAAACGCT"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 312..402"
                     /label="42BLA_PelB_s (mismatches: 0)"
     Primer_binding_ complement(312..402)
                     /note="Primer:
                     AGCGTTTCTGGGTGAGCGCTCACCGCGCTCGCCAGGCTCGCGGCCGCGGCCGCAAACA
                     GCAGTTTATAATGCATTTTTTGCCCTCGTTATC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(312..402)"
                     /label="43BLA_PelB_as (mismatches: 0)"
     Region          329..388
                     /note="Match:
                     ATGCATTATAAACTGCTGTTTGCGGCCGCGGCCGCGAGCCTGGCGAGCGCGGTGAGCG
                     CT"
                     /note="Length of match: 60"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label="PelB signal peptide"
     ORF             329..1855
                     /note="Length: 1503"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Primer_binding_ complement(368..391)
                     /note="Primer: cgcAGCGCTCACCGCGCTCGCCAG"
                     /note="Number of mismatches: 3"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(368..391)"
                     /label="442_PelB-AN_H-A_rev (mismatches: 3)"
     Primer_binding_ complement(375..397)
                     /note="Primer: cagcaGGTGAGCGCTCACCGCGC"
                     /note="Number of mismatches: 5"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(375..397)"
                     /label="435_PelB+LL  (mismatches: 5)"
     Primer_binding_ complement(375..391)
                     /note="Primer: GTGAGCGCTCACCGCGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(375..391)"
                     /label="52BLA_PelB+H_rev (mismatches: 0)"
     Region          389..904
                     /note="Match:
                     ATGCATTATAAACTGCTGTTTGCGGCCGCGGCCGCGAGCCTGGCGAGCGCGGTGAGCG
                     CTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGT
                     GGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
                     GAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCC
                     GTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTT
                     GGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
                     TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAA
                     CGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAAC
                     TCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
                     ACCACGACGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCACTAG"
                     /note="Length of match: 578"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=N-BLa_M180T
     Primer_binding_ 392..412
                     /note="Primer: CCAGAAACGCTGGTGAAAGTA"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 392..412"
                     /label="298BLA_delta_SP_fwd (mismatches: 0)"
     Primer_binding_ 398..420
                     /note="Primer: ACGCTGGTGAAAGTAAAAGATGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 398..420"
                     /label="436_N-BLA_fwd  (mismatches: 0)"
     Primer_binding_ 838..879
                     /note="Primer: CGACGAGCGTGACACCACGACGCCTGTAGCAATGGCAACAAC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 838..879"
                     /label="44BLA_M180T_s (mismatches: 0)"
     Primer_binding_ complement(838..879)
                     /note="Primer: GTTGTTGCCATTGCTACAGGCGTCGTGGTGTCACGCTCGTCG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(838..879)"
                     /label="45BLA_M180T_as (mismatches: 0)"
     Primer_binding_ 878..958
                     /note="Primer:
                     ggtggcgGCAgtggcggtggTGGCAgcgGcGGCgGtGGTTCTGCTAATCGAGCTAGC"
                     /note="Number of mismatches: 23"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 878..934"
                     /label="49Ia5GP_Spacer15x_fwd (mismatches: 23)"
     Primer_binding_ complement(880..901)
                     /note="Primer: GCCAGTTAATAGTTTGCGCAAC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(880..901)"
                     /label="50BLA_194G_rev (mismatches: 0)"
     Primer_binding_ complement(894..949)
                     /note="Primer: gcTAcCgccACCGCTGCCACTAGTGCCAGTTA"
                     /note="Number of mismatches: 6"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(894..925)"
                     /label="199N-sBLA_Spacer_GGGS_rev (mismatches: 6)"
     Primer_binding_ complement(894..937)
                     /note="Primer: GCTGCCACTAGTGCCAGTTA"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(894..913)"
                     /label="208N-BLA_Spacer_rev (mismatches: 0)"
     Primer_binding_ complement(895..961)
                     /note="Primer:
                     gctGCcgcCgCcgcTgcCgccgCCGCTGCCACTAGTGCCAGTT"
                     /note="Number of mismatches: 16"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(895..937)"
                     /label="207N-BLA_2xGGGS_rev (mismatches: 16)"
     Primer_binding_ 902..958
                     /note="Primer: ggTgGcGGtAGCGGTTCTGCTAATCGAGCTAGC"
                     /note="Number of mismatches: 5"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 902..934"
                     /label="198sBLA_Spacer_GGGS_fwd (mismatches: 5)"
     Region          905..943
                     /label="GGS linker"
     Primer_binding_ 929..958
                     /note="Primer: gGTGGCgGtGGTTCTGCTAATCGAGCTAGC"
                     /note="Number of mismatches: 3"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 905..934"
                     /label="212BLA_Spacer_+3_fwd (mismatches: 3)"
     Primer_binding_ 932..958
                     /note="Primer: GGtgGCGGTTCTGCTAATCGAGCTAGC"
                     /note="Number of mismatches: 2"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 908..934"
                     /label="211BLA_Spacer_+2_fwd (mismatches: 2)"
     Primer_binding_ 935..958
                     /note="Primer: gGtGGTTCTGCTAATCGAGCTAGC"
                     /note="Number of mismatches: 2"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 911..934"
                     /label="210BLA_Spacer_+1_fwd (mismatches: 2)"
     Primer_binding_ 938..958
                     /note="Primer: GGTTCTGCTAATCGAGCTAGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 914..934"
                     /label="197sBLA_Spacer_fwd (mismatches: 0)"
     Primer_binding_ complement(947..976)
                     /note="Primer: gcCCagCgcAcacAcCACGCTAGCTCGATT"
                     /note="Number of mismatches: 10"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(923..952)"
                     /label="304P01909-0_Q5-rev_02 (mismatches: 10)"
     Region          959..997
                     /label="ApaI-containing TMD"
     Region-detail   959..1000
                     /note="Match: GTGATCATTCTGGCGGTGGGGCCCGGCCTGCTGCTCCTGGGG"
                     /note="Length of match: 42"
                     /note="Accuracy (%): 100"
                     /note="Found at strand: Positive"
                     /label=alphaVmut-TMD
     Primer_binding_ complement(983..1057)
                     /note="Primer:
                     atCcgggcTCGgcagcGgTTtcaCAtcgctCgGgcttTTtttaatCAGgcgGcGGATC
                     CCCAGGAGCAGCAGGCC"
                     /note="Number of mismatches: 36"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(959..1033)"
                     /label="258Cterm_GpA- (mismatches: 36)"
     Primer_binding_ 989..1016
                     /note="Primer: gcagcggcaGGGATCCACAAACTGGCAG"
                     /note="Number of mismatches: 9"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 965..992"
                     /label="129GpAelongate+3A+ (mismatches: 9)"
     Primer_binding_ complement(991..1067)
                     /note="Primer:
                     CccgttgAatatggctacTaGtKcYKcYKcYKcYkcykcykcykcYkcYkcYKcYkcy
                     gaTcaGGATCCCCAGGAGC"
                     /note="Number of mismatches: 45"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(967..1043)"
                     /label="33IaGP-NC_12x_as (mismatches: 45)"
     Primer_binding_ 992..1016
                     /note="Primer: gcggcaGGGATCCACAAACTGGCAG"
                     /note="Number of mismatches: 6"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 968..992"
                     /label="128GpAelongate+2A+ (mismatches: 6)"
     Primer_binding_ 995..1016
                     /note="Primer: gcaGGGATCCACAAACTGGCAG"
                     /note="Number of mismatches: 3"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 971..992"
                     /label="127GpAelongate+1A+ (mismatches: 3)"
     Region          1007..1117
                     /label="rigid linker"
     Primer_binding_ 1010..1030
                     /note="Primer: CTGGCAGTTAATGTTCAGCTG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 986..1006"
                     /label="300BLA_delta_TM_fwd (mismatches: 0)"
     Primer_binding_ complement(1010..1033)
                     /note="Primer: AGGCAGCTGAACATTAACTGCCAG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(986..1009)"
                     /label="266deltaLinkerrev (mismatches: 0)"
     Primer_binding_ complement(1016..1037)
                     /note="Primer: CGGCAGGCAGCTGAACATTAAC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(992..1013)"
                     /label="92sfGFP-linker_rev (mismatches: 0)"
     Primer_binding_ 1019..1051
                     /note="Primer: cgctggaAagTGaagGCCGAAGCAGCAGCGAAA"
                     /note="Number of mismatches: 12"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 995..1027"
                     /label="132QSOX2nativeC-term+ (mismatches: 12)"
     Primer_binding_ 1061..1142
                     /note="Primer:
                     aCCgAtGtgcCGctgagcAgcGtGGaaattGaaAAcccGGaAaCcagcgAtcagGCAA
                     GCAAAGGTGAAGAACTGTTTACCG"
                     /note="Number of mismatches: 36"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1037..1118"
                     /label="257Cterm_GpA+ (mismatches: 36)"
     Primer_binding_ complement(1093..1129)
                     /note="Primer: gctACtgaaatccaCGGCTTTAGCTGCTGCCTCTTTC"
                     /note="Number of mismatches: 12"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1069..1105)"
                     /label="131QSOX2nativeN-term- (mismatches: 12)"
     Primer_binding_ 1096..1139
                     /note="Primer:
                     tttaagAaggagatAtaCCatgAGCAAAGGTGAAGAACTGTTTA"
                     /note="Number of mismatches: 18"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1072..1115"
                     /label="318LIC16_GFP_fwd (mismatches: 18)"
     Primer_binding_ 1097..1142
                     /note="Primer:
                     tgGGCtagcaggAggatgGttAGCAAgGGcGAgGAgCTGTTTACCG"
                     /note="Number of mismatches: 20"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1073..1118"
                     /label="100MT027 (mismatches: 20)"
     Primer_binding_ 1106..1142
                     /note="Primer: agTcgtcatatgAGCAAAGGTGAAGAACTGTTTACCG"
                     /note="Number of mismatches: 11"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1082..1118"
                     /label="113MT033_fwd_sfGFP (mismatches: 11)"
     Primer_binding_ 1112..1142
                     /note="Primer: catatgAGCAAAGGTGAAGAACTGTTTACCG"
                     /note="Number of mismatches: 6"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1088..1118"
                     /label="106MT030_fwd_sfGFP (mismatches: 6)"
     Primer_binding_ 1115..1140
                     /note="Primer: GCAAGCAAAGGTGAAGAACTGTTTAC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1091..1116"
                     /label="265deltaLinkerfrw (mismatches: 0)"
     Region          1118..1828
                     /note="Match:
                     GATCCACAAACTGGCAGTTAATGTTCAGCTGCCTGCCGAAGCAGCAGCGAAAGAAGCC
                     GCTGCCAAAGAAGCGGCAGCGAAAGAGGCTGCCGCGAAAGAGGCAGCAGCTAAAGCCG
                     CAAGCAAAGGTGAAGAACTGTTTACCGGTGTTGTTCCGATTCTGGTTGAACTGGATGG
                     TGATGTTAATGGCCACAAATTCTCAGTTCGTGGTGAAGGCGAAGGTGATGCAACCAAT
                     GGTAAACTGACCCTGAAATTTATCTGTACCACCGGCAAACTGCCGGTTCCGTGGCCGA
                     CCCTGGTTACCACCCTGACCTATGGTGTTCAGTGTTTCAGCCGTTATCCGGATCATAT
                     GAAACAGCACGATTTCTTCAAATCTGCAATGCCGGAAGGTTATGTTCAAGAACGTACC
                     ATCTCCTTTAAAGATGATGGCACCTATAAGACCCGTGCCGAAGTTAAATTTGAAGGTG
                     ATACCCTGGTGAATCGCATTGAACTGAAAGGCATCGATTTCAAAGAAGATGGTAATAT
                     CCTGGGCCATAAACTGGAATATAATTTCAACAGCCACAACGTGTATATCACCGCAGAT
                     AAACAGAAGAATGGCATCAAAGCCAACTTTAAGATCCGCCATAATGTTGAAGATGGCA
                     GCGTTCAGCTGGCAGATCATTATCAGCAGAATACACCGATTGGTGATGGTCCGGTTCT
                     GCTGCCGGATAATCATTATCTGAGCACCCAGAGCGTTCTGAGCAAAGATCCGAATGAG
                     AAACGTGATCACATGGTGCTGCTGGAATTTGTTACCGCAGCAGGTATTACCCATGGTA
                     TGGATGAACTGTAT"
                     /note="Length of match: 826"
                     /note="Accuracy (%): 100"
                     /note="Description: super folded GFP with linker region"
                     /note="Found at strand: Positive"
                     /label=sfGFP
     Region-detail   1130..1132
                     /note="Match: GAAAGAGGCAGCAGCTAAAGCCGCAAGCAAAGGTGA"
                     /note="Length of match: 36"
                     /note="Accuracy (%): 100"
                     /note="Description: Glu->Gly PCR induced mut (SKGGEL not
                     SKGEEL), GFP function unaffected, Patrick linker cloning"
                     /note="Found at strand: Positive"
                     /label="Glu->Gly PCR induced mut (SKGGEL not SKGEEL), GFP function unaffected"
     Primer_binding_ 1135..1160
                     /note="Primer: GTTTACCGGTGTTGTTCCGATTCTGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1111..1136"
                     /label="201MT036_fwd_cfSGFP2 (mismatches: 0)"
     Primer_binding_ 1145..1178
                     /note="Primer: GTatgTggcAagCTctgcGtttctGATGGTGATG"
                     /note="Number of mismatches: 17"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1121..1154"
                     /label="166Q6ZRP7L10A+ (mismatches: 17)"
     Primer_binding_ complement(1291..1310)
                     /note="Primer: gGGTCAGGGTGGTAACCAGG"
                     /note="Number of mismatches: 1"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1267..1286)"
                     /label="96BFP_Y66H_rev (mismatches: 1)"
     Primer_binding_ 1298..1336
                     /note="Primer: ACCACCCTGACCTATGcTGTTCAGTGTTTCAGCCGTTAT"
                     /note="Number of mismatches: 1"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1274..1312"
                     /label="120sfGFPG67A+ (mismatches: 1)"
     Primer_binding_ complement(1298..1336)
                     /note="Primer: ATAACGGCTGAAACACTGAACAgCATAGGTCAGGGTGGT"
                     /note="Number of mismatches: 1"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1274..1312)"
                     /label="121sfGFPG67A- (mismatches: 1)"
     Primer_binding_ 1311..1331
                     /note="Primer: ATGGTGTTCAGTGTTTCAGCC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1287..1307"
                     /label="95BFP_Y66H_fwd (mismatches: 0)"
     Primer_binding_ complement(1332..1358)
                     /note="Primer: CGTGCTGTTTCATgTGATCCGGATAAC"
                     /note="Number of mismatches: 1"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1308..1334)"
                     /label="111sfGFP_PDHMKQ_rev (mismatches: 1)"
     Primer_binding_ complement(1661..1711)
                     /note="Primer:
                     gTGATTATCCGGCAGCAGAACCGGACCATCACCAATCGGgGTATTCTGCTG"
                     /note="Number of mismatches: 2"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 1"
                     /note="Primer binding region: complement(1637..1687)"
                     /label="302cfSGFP2_fix_indel_rev (mismatches: 2)"
     Region          1832..1852
                     /note="Match: GGCGATTATAAAGATCACGACGGC"
                     /note="Length of match: 24"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=FLAG-Tag
     Primer_binding_ complement(1832..1881)
                     /note="Primer:
                     gcGgccgcGTcGactTaaaGCTtactagtGCCGTCGTGATCTTTATAATC"
                     /note="Number of mismatches: 21"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1808..1857)"
                     /label="107MT031_rev_sfGFP (mismatches: 21)"
     Primer_binding_ complement(1832..1852)
                     /note="Primer: GCCGTCGTGATCTTTATAATC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1808..1828)"
                     /label="97FLAG_rev (mismatches: 0)"
     Primer_binding_ complement(1862..1912)
                     /note="Primer:
                     gatCagcgGGttTaaacggGCcctCtagaCTCAcacGgccgGGTGGTGGTG"
                     /note="Number of mismatches: 29"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 1"
                     /note="Primer binding region: complement(1838..1888)"
                     /label="262QSOX2_pcDNA3.1rev (mismatches: 29)"
     Primer_binding_ complement(1868..1918)
                     /note="Primer:
                     gatcagcgggTTTaaacgggcccTctagaCtcacaCggccgGGTGGTGGTG"
                     /note="Number of mismatches: 35"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 1"
                     /note="Primer binding region: complement(1844..1894)"
                     /label="262QSOX2_pcDNA3.1rev (mismatches: 35)"
     Primer_binding_ complement(1932..1950)
                     /note="Primer: GCTAGTTATTGCTCAGCGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1908..1926)"
                     /label="primer_Seq-T7-Seqdn (mismatches: 0)"
     Primer_binding_ complement(1932..1949)
                     /note="Primer: CTAGTTATTGCTCAGCGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1908..1925)"
                     /label="primer_GATC-pET-RP (mismatches: 0)"
     Primer_binding_ complement(1934..1952)
                     /note="Primer: ATGCTAGTTATTGCTCAGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1910..1928)"
                     /label="primer_GATC-pRSET-RP (mismatches: 0)"
     Primer_binding_ complement(1935..1957)
                     /note="Primer: GGGTTATGCTAGTTATTGCTCAG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1911..1933)"
                     /label="primer_GATC-pET-24a (mismatches: 0)"
     Primer_binding_ complement(1935..1957)
                     /note="Primer: GGGTTATGCTAGTTATTGCTCAG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1911..1933)"
                     /label="110T7_terminator_rev (mismatches: 0)"
     Primer_binding_ complement(1939..1957)
                     /note="Primer: GGGTTATGCTAGTTATTGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1915..1933)"
                     /label="primer_GATC-pRSET-RPnew (mismatches: 0)"
     Region          1951..1994
                     /note="Match:
                     ATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGA"
                     /note="Length of match: 44"
                     /note="Accuracy (%): 100"
                     /note="Description: F04_checksum."
                     /note="Found at strand: Positive"
                     /label=T7_terminator
     Primer_binding_ complement(1965..1984)
                     /note="Primer: CCTCAAGACCCGTTTAGAGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1941..1960)"
                     /label="432_pT7_dNgoMIV_rev  (mismatches: 0)"
     Primer_binding_ complement(1993..2010)
                     /note="Primer: CcgTCAAGGGCATCGGTC"
                     /note="Number of mismatches: 2"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1969..1986)"
                     /label="primer_Seq-BiTermin800rev_Jana (mismatches: 2)"
     Region          1998..2612
                     /note="Match:
                     ATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTA
                     TCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGC
                     AGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGC
                     CTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTC
                     CCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGC
                     GCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATG
                     ATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGC
                     AGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCT
                     AACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACA
                     TGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGT
                     TGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACC"
                     /note="Length of match: 615"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label="TetR (apparrently deactivated by Lindner)"
     ORF             1998..2615
                     /note="Length: 618"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Primer_binding_ complement(2012..2029)
                     /note="Primer: GAGCTGACTGGGTTGAAG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1988..2005)"
                     /label="primer_GATC-pGL3-1 (mismatches: 0)"
     Region          2854..3352
                     /label="p15a ori possible region"
     Primer_binding_ 3229..3247
                     /note="Primer: CACGGGGCCTGCCACCATA"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 3205..3223"
                     /label="431_pT7_dNgoMIV_fwd  (mismatches: 0)"
     ORF             complement(3649..4308)
                     /note="Length: 660"
                     /note="Found at strand: negative"
                     /note="Start codon: ATG"
     Region          complement(3652..4308)
                     /note="Match:
                     ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAG
                     AACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCT
                     GGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCC
                     TTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGA
                     AAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGA
                     GCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTT
                     CTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTA
                     AAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAG
                     TTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGC
                     AAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATG
                     CCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGA
                     TGAGTGGCAGGGCGGGGCG"
                     /note="Length of match: 657"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Negative"
                     /label=CAT(CmR)
     Primer_binding_ 3658..3673
                     /note="Primer: GCCCTGCCACTCATCG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 3634..3649"
                     /label="primer_GATC-pTeSp-2 (mismatches: 0)"
     Primer_binding_ complement(4768..4818)
                     /note="Primer:
                     gtgaTtAtccgGCagcagAaCcGgAccatcaCcaatcggggTATTCTGCTG"
                     /note="Number of mismatches: 32"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 1"
                     /note="Primer binding region: complement(4744..4794)"
                     /label="302cfSGFP2_fix_indel_rev (mismatches: 32)"
     Primer_binding_ 4854..4918
                     /note="Primer:
                     ccCgTtgaAtatGGctcataAcacCCcttgtatTaCggtaccGATACTTAACAGGGAA
                     GTGAGAG"
                     /note="Number of mismatches: 32"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 4830..4894"
                     /label="3p15a_fwd_KpnI_Ecol1 (mismatches: 32)"
     Primer_binding_ 4893..4918
                     /note="Primer: tcctAggCTTAACAGGGAAGTGAGAG"
                     /note="Number of mismatches: 6"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 4869..4894"
                     /label="9p15a_fwd_AvrII (mismatches: 6)"
     Primer_binding_ 5067..5084
                     /note="Primer: CCTGCCTTTCGGTTTACC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 5043..5060"
                     /label="5p15a_int_fwd (mismatches: 0)"
     Primer_binding_ complement(5367..5383)
                     /note="Primer: GAACCGAGGTAACTGGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(5343..5359)"
                     /label="6p15a_int_rev (mismatches: 0)"
     Primer_binding_ complement(5462..5520)
                     /note="Primer:
                     cagggGAtAacgcaggaaaGAacAtgtgagcGAatTcCTTGAGATCGTTTTGGTCTGC
                     G"
                     /note="Number of mismatches: 28"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(5438..5496)"
                     /label="4p15a_rev_EcoRI_Ecol1 (mismatches: 28)"
     Primer_binding_ complement(5464..5503)
                     /note="Primer: ggaATTccatAtggaATtccCTTGAGATCGTTTTGGTCTG"
                     /note="Number of mismatches: 14"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(5440..5479)"
                     /label="10p15a_rev_NdeI (mismatches: 14)"
     Primer_binding_ complement(5480..5504)
                     /note="Primer: TagaAaagATcAaAgGATCTTCTTG"
                     /note="Number of mismatches: 9"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(5456..5480)"
                     /label="268ColEI-pcDNArev (mismatches: 9)"
     Primer_binding_ complement(5510..5534)
                     /note="Primer: TAAATTGCACTGAAATCTAGAAATA"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(5486..5510)"
                     /label="primer_GATC-TET-1_RP-1 (mismatches: 0)"
     Primer_binding_ 5544..5564
                     /note="Primer: ccGTAGCACCTGAAGTCAGCC"
                     /note="Number of mismatches: 2"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 5520..5540"
                     /label="primer_Seq-BiPromotor700fwd_Jana (mismatches: 2)"
     Primer_binding_ 5546..5566
                     /note="Primer: GTAGCACCTGAAGTCAGCCCC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 5522..5542"
                     /label="primer_Seq-pEL_Isequ_Up (mismatches: 0)"
ORIGIN
        1 ATCGATAAGA AACCAATTGT CCATATTGCA TCAGACATTG CCGTCACTGC GTCTTTTACT
       61 GGCTCTTCTC GCTAACCAAA CCGGTAACCC CGCTTATTAA AAGCATTCTG TAACAAAGCG
      121 GGACCAAAGC CATGACAAAA ACGCGTAACA AAAGTGTCTA TAATCACGGC AGAAAAGTCC
      181 ACATTGATTA TTTGCACGGC GTCACACTTT GCTATGCCAT AGCATTTTTA TCCATAAGAT
      241 TAGCGGATGC TACCTGACGC TTTTTATCGC AACTCTCTAC TGTTTCTCCA TACCCGTTTT
      301 TTTGGGCTCT AGATAACGAG GGCAAAAAAT GCATTATAAA CTGCTGTTTG CGGCCGCGGC
      361 CGCGAGCCTG GCGAGCGCGG TGAGCGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC
      421 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT
      481 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT
      541 ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA
      601 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG
      661 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA
      721 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG
      781 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA
      841 CGAGCGTGAC ACCACGACGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG
      901 CACTAGTGGC AGCGGCGGCG GCAGCGGCGG CGGCAGCGGT TCTGCTAATC GAGCTAGCGT
      961 GATCATTCTG GCGGTGGGGC CCGGCCTGCT GCTCCTGGGG ATCCACAAAC TGGCAGTTAA
     1021 TGTTCAGCTG CCTGCCGAAG CAGCAGCGAA AGAAGCCGCT GCCAAAGAAG CGGCAGCGAA
     1081 AGAGGCTGCC GCGAAAGAGG CAGCAGCTAA AGCCGCAAGC AAAGGTGgAG AACTGTTTAC
     1141 CGGTGTTGTT CCGATTCTGG TTGAACTGGA TGGTGATGTT AATGGCCACA AATTCTCAGT
     1201 TCGTGGTGAA GGCGAAGGTG ATGCAACCAA TGGTAAACTG ACCCTGAAAT TTATCTGTAC
     1261 CACCGGCAAA CTGCCGGTTC CGTGGCCGAC CCTGGTTACC ACCCTGACCT ATGGTGTTCA
     1321 GTGTTTCAGC CGTTATCCGG ATCATATGAA ACAGCACGAT TTCTTCAAAT CTGCAATGCC
     1381 GGAAGGTTAT GTTCAAGAAC GTACCATCTC CTTTAAAGAT GATGGCACCT ATAAGACCCG
     1441 TGCCGAAGTT AAATTTGAAG GTGATACCCT GGTGAATCGC ATTGAACTGA AAGGCATCGA
     1501 TTTCAAAGAA GATGGTAATA TCCTGGGCCA TAAACTGGAA TATAATTTCA ACAGCCACAA
     1561 CGTGTATATC ACCGCAGATA AACAGAAGAA TGGCATCAAA GCCAACTTTA AGATCCGCCA
     1621 TAATGTTGAA GATGGCAGCG TTCAGCTGGC AGATCATTAT CAGCAGAATA CACCGATTGG
     1681 TGATGGTCCG GTTCTGCTGC CGGATAATCA TTATCTGAGC ACCCAGAGCG TTCTGAGCAA
     1741 AGATCCGAAT GAGAAACGTG ATCACATGGT GCTGCTGGAA TTTGTTACCG CAGCAGGTAT
     1801 TACCCATGGT ATGGATGAAC TGTATAAAGG CGATTATAAA GATCACGACG GCTAACTCGA
     1861 GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT
     1921 GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT
     1981 GAGGGGTTTT TTGACCGATG CCCTTGAGAG CCTTCAACCC AGTCAGCTCC TTCCGGTGGG
     2041 CGCGGGGCAT GACTATCGTC GCCGCACTTA TGACTGTCTT CTTTATCATG CAACTCGTAG
     2101 GACAGGTGCC GGCAGCGCTC TGGGTCATTT TCGGCGAGGA CCGCTTTCGC TGGAGCGCGA
     2161 CGATGATCGG CCTGTCGCTT GCGGTATTCG GAATCTTGCA CGCCCTCGCT CAAGCCTTCG
     2221 TCACTGGTCC CGCCACCAAA CGTTTCGGCG AGAAGCAGGC CATTATCGCC GGCATGGCGG
     2281 CCGACGCGCT GGGCTACGTC TTGCTGGCGT TCGCGACGCG AGGCTGGATG GCCTTCCCCA
     2341 TTATGATTCT TCTCGCTTCC GGCGGCATCG GGATGCCCGC GTTGCAGGCC ATGCTGTCCA
     2401 GGCAGGTAGA TGACGACCAT CAGGGACAGC TTCAAGGATC GCTCGCGGCT CTTACCAGCC
     2461 TAACTTCGAT CACTGGACCG CTGATCGTCA CGGCGATTTA TGCCGCCTCG GCGAGCACAT
     2521 GGAACGGGTT GGCATGGATT GTAGGCGCCG CCCTATACCT TGTCTGCCTC CCCGCGTTGC
     2581 GTCGCGGTGC ATGGAGCCGG GCCACCTCGA CCTGAATGGA AGCCGGCGGC ACCTCGCTAA
     2641 CGGATTCACC ACTCCAAGAA TTGGAGCCAA TCAATTCTTG CGGAGAACTG TGAATGCGCA
     2701 AACCAACCCT TGGCAGAACA TATCCATCGC GTCCGCCATC TCCAGCAGCC GCACGCGGCG
     2761 CATCTCGGGC AGCGTTGGGT CCTGGCCACG GGTGCGCATG ATCGTGCTCC TGTCGTTGAG
     2821 GACCCGGCTA GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC CGATACGCGA
     2881 GCGAACGTGA AGCGACTGCT GCTGCAAAAC GTCTGCGACC TGAGCAACAA CATGAATGGT
     2941 CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA AACGCGGAAG TCCCCTACGT GCTGCTGAAG
     3001 TTGCCCGCAA CAGAGAGTGG AACCAACCGG TGATACCACG ATACTATGAC TGAGAGTCAA
     3061 CGCCATGAGC GGCCTCATTT CTTATTCTGA GTTACAACAG TCCGCACCGC TGTCCGGTAG
     3121 CTCCTTCCGG TGGGCGCGGG GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT
     3181 CATGCAACTC GTAGGACAGG TGCCGGCAGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC
     3241 CACCATACCC ACGCCGAAAC AAGCGCCCTG CACCATTATG TTCCGGATCT GCATCGCAGG
     3301 ATGCTGCTGG CTACCCTGTG GAACACCTAC ATCTGTATTA ACGAAGCGCT AACCGTTTTT
     3361 ATCAGGCTCT GGGAGGCAGA ATAAATGATC ATATCGTCAA TTATTACCTC CACGGGGAGA
     3421 GCCTGAGCAA ACTGGCCTCA GGCATTTGAG AAGCACACGG TCACACTGCT TCCGGTAGTC
     3481 AATAAACCGG TAAACCAGCA ATAGACATAA GCGGCTATTT AACGACCCTG CCCTGAACCG
     3541 ACGACCGGGT CGAATTTGCT TTCGAATTTC TGCCATTCAT CCGCTTATTA TCACTTATTC
     3601 AGGCGTAGCA CCAGGCGTTT AAGGGCACCA ATAACTGCCT TAAAAAAATT ACGCCCCGCC
     3661 CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC
     3721 ACAGACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA
     3781 ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC
     3841 AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC
     3901 TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG
     3961 AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC
     4021 ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT
     4081 TGCCATACGG AATTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG
     4141 ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC
     4201 GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG
     4261 CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATTT TAGCTTCCTT
     4321 AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT AGTGATCTTA TTTCATTATG
     4381 GTGAAAGTTG GAACCTCTTA CGTGCCGATC AACGTCTCAT TTTCGCCAAA AGTTGGCCCA
     4441 GGGCTTCCCG GTATCAACAG GGACACCAGG ATTTATTTAT TCTGCGAAGT GATCTTCCGT
     4501 CACAGGTATT TATTCGGCGC AAAGTGCGTC GGGTGATGCT GCCAACTTAC TGATTTAGTG
     4561 TATGATGGTG TTTTTGAGGT GCTCCAGTGG CTTCTGTTTC TATCAGCTGT CCCTCCTGTT
     4621 CAGCTACTGA CGGGGTGGTG CGTAACGGCA AAAGCACCGC CGGACATCAG CGCTGGCGGA
     4681 GTGTATACTG GCTTACTATG TTGGCACTGA TGAGGGTGTC AGTGAAGTGC TTCATGTGGC
     4741 AGGAGAAAAA AGGCTGCACC GGTGCGTCAG CAGAATATGT GATACAGGAT ATATTCCGCT
     4801 TCCTCGCTCA CTGACTCGCT ACGCTCGGTC GTTCGACTGC GGCGAGCGGA AATGGCTTAC
     4861 GAACGGGGCG GAGATTTCCT GGAAGATGCC AGGAAGATAC TTAACAGGGA AGTGAGAGGG
     4921 CCGCGGCAAA GCCGTTTTTC CATAGGCTCC GCCCCCCTGA CAAGCATCAC GAAATCTGAC
     4981 GCTCAAATCA GTGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
     5041 GCGGCTCCCT CGTGCGCTCT CCTGTTCCTG CCTTTCGGTT TACCGGTGTC ATTCCGCTGT
     5101 TATGGCCGCG TTTGTCTCAT TCCACGCCTG ACACTCAGTT CCGGGTAGGC AGTTCGCTCC
     5161 AAGCTGGACT GTATGCACGA ACCCCCCGTT CAGTCCGACC GCTGCGCCTT ATCCGGTAAC
     5221 TATCGTCTTG AGTCCAACCC GGAAAGACAT GCAAAAGCAC CACTGGCAGC AGCCACTGGT
     5281 AATTGATTTA GAGGAGTTAG TCTTGAAGTC ATGCGCCGGT TAAGGCTAAA CTGAAAGGAC
     5341 AAGTTTTGGT GACTGCGCTC CTCCAAGCCA GTTACCTCGG TTCAAAGAGT TGGTAGCTCA
     5401 GAGAACCTTC GAAAAACCGC CCTGCAAGGC GGTTTTTTCG TTTTCAGAGC AAGAGATTAC
     5461 GCGCAGACCA AAACGATCTC AAGAAGATCA TCTTATTAAT CAGATAAAAT ATTTCTAGAT
     5521 TTCAGTGCAA TTTATCTCTT CAAATGTAGC ACCTGAAGTC AGCCCCATAC GATATAAGTT
     5581 GTAATTCTCA TGTTTGACAG CTTATCA
//
