LOCUS       549_N-BLA_1.2(pToxRVII_N-BLA(OmpA)M180T-sfGFP)_corrected      5610 bp    DNA     circular UNA 
DEFINITION  araBAD-Promoter anti aIIb
FEATURES             Location/Qualifiers
     important_prime 69..88
                     /note="Primer: GCATTCTGTAACAAAGCGGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     549_N-BLA_1.2(pToxRVII_N-BLA(OmpA)M180T-sfGFP): 0"
                     /note="Primer binding region: 69..88"
                     /label="57_pToxRV_fwd(ToxSeqUp) (mismatches: 0)"
     Region          133..144
                     /note="Match: CGGCAGAAAAGT"
                     /note="Length of match: 12"
                     /note="Accuracy (%): 100"
                     /note="Description: 1540_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_O1_operator_region[REG]{araO1},12_bases,
     Region          175..188
                     /note="Match: TTGCTATGCCATAG"
                     /note="Length of match: 14"
                     /note="Accuracy (%): 100"
                     /note="Description: 1D6E_checksum."
                     /note="Found at strand: Positive"
                     /label=CAP_binding_site[OTH]{CAP_BS},14_bases,
     Region          220..248
                     /note="Match: CTGACGCTTTTTATCGCAACTCTCTACTG"
                     /note="Length of match: 29"
                     /note="Accuracy (%): 100"
                     /note="Description: 7B4_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_minimal_promoter[PRO]{ARA},29_bases,
     ORF             295..1821
                     /note="Length: 1527"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Region          295..360
                     /note="Match:
                     ATGAAAAAGACCGCGATTGCGATTGCGGTGGCGCTGGCGGGCTTTGCGACCGTGGCGC
                     AGGCGGCG"
                     /note="Length of match: 66"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=OmpA_SP
     important_prime complement(388..406)
                     /note="Primer: CACCCAACTGATCTTCAGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     549_N-BLA_1.2(pToxRVII_N-BLA(OmpA)M180T-sfGFP): 0"
                     /note="Primer binding region: complement(388..406)"
                     /label="752_N_BLA_col_rev (mismatches: 0)"
     important_prime 822..840
                     /note="Primer: CACGACGCCTGTAGCAATG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     549_N-BLA_1.2(pToxRVII_N-BLA(OmpA)M180T-sfGFP): 0"
                     /note="Primer binding region: 822..840"
                     /label="750_N-BLA_fwd (mismatches: 0)"
     Region          969..1794
                     /note="Match:
                     GATCCACAAACTGGCAGTTAATGTTCAGCTGCCTGCCGAAGCAGCAGCGAAAGAAGCC
                     GCTGCCAAAGAAGCGGCAGCGAAAGAGGCTGCCGCGAAAGAGGCAGCAGCTAAAGCCG
                     CAAGCAAAGGTGAAGAACTGTTTACCGGTGTTGTTCCGATTCTGGTTGAACTGGATGG
                     TGATGTTAATGGCCACAAATTCTCAGTTCGTGGTGAAGGCGAAGGTGATGCAACCAAT
                     GGTAAACTGACCCTGAAATTTATCTGTACCACCGGCAAACTGCCGGTTCCGTGGCCGA
                     CCCTGGTTACCACCCTGACCTATGGTGTTCAGTGTTTCAGCCGTTATCCGGATCATAT
                     GAAACAGCACGATTTCTTCAAATCTGCAATGCCGGAAGGTTATGTTCAAGAACGTACC
                     ATCTCCTTTAAAGATGATGGCACCTATAAGACCCGTGCCGAAGTTAAATTTGAAGGTG
                     ATACCCTGGTGAATCGCATTGAACTGAAAGGCATCGATTTCAAAGAAGATGGTAATAT
                     CCTGGGCCATAAACTGGAATATAATTTCAACAGCCACAACGTGTATATCACCGCAGAT
                     AAACAGAAGAATGGCATCAAAGCCAACTTTAAGATCCGCCATAATGTTGAAGATGGCA
                     GCGTTCAGCTGGCAGATCATTATCAGCAGAATACACCGATTGGTGATGGTCCGGTTCT
                     GCTGCCGGATAATCATTATCTGAGCACCCAGAGCGTTCTGAGCAAAGATCCGAATGAG
                     AAACGTGATCACATGGTGCTGCTGGAATTTGTTACCGCAGCAGGTATTACCCATGGTA
                     TGGATGAACTGTAT"
                     /note="Length of match: 826"
                     /note="Accuracy (%): 100"
                     /note="Description: super folded GFP with linker region"
                     /note="Found at strand: Positive"
                     /label=sfGFP
     important_prime complement(1260..1279)
                     /note="Primer: gGGTCAGGGTGGTAACCAGG"
                     /note="Number of mismatches: 1"
                     /note="Number of other hits on
                     549_N-BLA_1.2(pToxRVII_N-BLA(OmpA)M180T-sfGFP): 0"
                     /note="Primer binding region: complement(1260..1279)"
                     /label="96_BFP_Y66H_rev (mismatches: 1)"
     Region          1798..1821
                     /note="Match: GGCGATTATAAAGATCACGACGGC"
                     /note="Length of match: 24"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=FLAG-Tag
     Region          1920..1963
                     /note="Match:
                     ATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGA"
                     /note="Length of match: 44"
                     /note="Accuracy (%): 100"
                     /note="Description: F04_checksum."
                     /note="Found at strand: Positive"
                     /label=T7_terminator
     Region          1967..2581
                     /note="Match:
                     ATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTA
                     TCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGC
                     AGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGC
                     CTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTC
                     CCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGC
                     GCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATG
                     ATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGC
                     AGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCT
                     AACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACA
                     TGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGT
                     TGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACC"
                     /note="Length of match: 615"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=TetR_CDS
     ORF             3019..3318
                     /note="Length: 300"
                     /note="Found at strand: positive"
                     /note="Start codon: CTG"
     Region          complement(3621..4277)
                     /note="Match:
                     ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAG
                     AACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCT
                     GGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCC
                     TTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGA
                     AAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGA
                     GCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTT
                     CTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTA
                     AAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAG
                     TTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGC
                     AAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATG
                     CCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGA
                     TGAGTGGCAGGGCGGGGCG"
                     /note="Length of match: 657"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Negative"
                     /label=CAT_CDS
     Region          complement(4709..4909)
                     /note="Match:
                     GAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTT
                     CCAGGAAATCTCCGCCCCGTTCGTAAGCCATTTCCGCTCGCCGCAGTCGAACGACCGA
                     GCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCTGTATCACATATTCTGCTGA
                     CGCACCGGTGCAGCCTTTTTTCTCCTG"
                     /note="Length of match: 201"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Negative"
                     /label=p15a_ORI
     Region          5586..5601
                     /note="Match: AAACCAATTGTCCATA"
                     /note="Length of match: 16"
                     /note="Accuracy (%): 100"
                     /note="Description: 2639_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_O2_operator_region[REG]{araO2},16_bases,
ORIGIN
        1 ACATTGCCGT CACTGCGTCT TTTACTGGCT CTTCTCGCTA ACCAAACCGG TAACCCCGCT
       61 TATTAAAAGC ATTCTGTAAC AAAGCGGGAC CAAAGCCATG ACAAAAACGC GTAACAAAAG
      121 TGTCTATAAT CACGGCAGAA AAGTCCACAT TGATTATTTG CACGGCGTCA CACTTTGCTA
      181 TGCCATAGCA TTTTTATCCA TAAGATTAGC GGATGCTACC TGACGCTTTT TATCGCAACT
      241 CTCTACTGTT TCTCCATACC CGTTTTTTTG GGCTCTAGAT AACGAGGGCA AAAAATGAAA
      301 AAGACCGCGA TTGCGATTGC GGTGGCGCTG GCGGGCTTTG CGACCGTGGC GCAGGCGGCG
      361 CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC
      421 ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT
      481 CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC
      541 GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA
      601 CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC
      661 ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG
      721 GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA
      781 CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGACGCC TGTAGCAATG
      841 GCAACAACGT TGCGCAAACT ATTAACTGGC ACTAGTGGCA GCGGCGGCGG CAGCGGCGGC
      901 GGCAGCGGTT CTGCTAATCG AGCTAGCGTG ATCATTCTGG CGGTGGGGCC CGGCCTGCTG
      961 CTCCTGGGGA TCCACAAACT GGCAGTTAAT GTTCAGCTGC CTGCCGAAGC AGCAGCGAAA
     1021 GAAGCCGCTG CCAAAGAAGC GGCAGCGAAA GAGGCTGCCG CGAAAGAGGC AGCAGCTAAA
     1081 GCCGCAAGCA AAGGTGgAGA ACTGTTTACC GGTGTTGTTC CGATTCTGGT TGAACTGGAT
     1141 GGTGATGTTA ATGGCCACAA ATTCTCAGTT CGTGGTGAAG GCGAAGGTGA TGCAACCAAT
     1201 GGTAAACTGA CCCTGAAATT TATCTGTACC ACCGGCAAAC TGCCGGTTCC GTGGCCGACC
     1261 CTGGTTACCA CCCTGACCTA TGGTGTTCAG TGTTTCAGCC GTTATCCGGA TCATATGAAA
     1321 CAGCACGATT TCTTCAAATC TGCAATGCCG GAAGGTTATG TTCAAGAACG TACCATCTCC
     1381 TTTAAAGATG ATGGCACCTA TAAGACCCGT GCCGAAGTTA AATTTGAAGG TGATACCCTG
     1441 GTGAATCGCA TTGAACTGAA AGGCATCGAT TTCAAAGAAG ATGGTAATAT CCTGGGCCAT
     1501 AAACTGGAAT ATAATTTCAA CAGCCACAAC GTGTATATCA CCGCAGATAA ACAGAAGAAT
     1561 GGCATCAAAG CCAACTTTAA GATCCGCCAT AATGTTGAAG ATGGCAGCGT TCAGCTGGCA
     1621 GATCATTATC AGCAGAATAC ACCGATTGGT GATGGTCCGG TTCTGCTGCC GGATAATCAT
     1681 TATCTGAGCA CCCAGAGCGT TCTGAGCAAA GATCCGAATG AGAAACGTGA TCACATGGTG
     1741 CTGCTGGAAT TTGTTACCGC AGCAGGTATT ACCCATGGTA TGGATGAACT GTATAAAGGC
     1801 GATTATAAAG ATCACGACGG CTAACTCGAG CACCACCACC ACCACCACTG AGATCCGGCT
     1861 GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA
     1921 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGACCGATGC CCTTGAGAGC
     1981 CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTATCGTCG CCGCACTTAT
     2041 GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT GGGTCATTTT
     2101 CGGCGAGGAC CGCTTTCGCT GGAGCGCGAC GATGATCGGC CTGTCGCTTG CGGTATTCGG
     2161 AATCTTGCAC GCCCTCGCTC AAGCCTTCGT CACTGGTCCC GCCACCAAAC GTTTCGGCGA
     2221 GAAGCAGGCC ATTATCGCCG GCATGGCGGC CGACGCGCTG GGCTACGTCT TGCTGGCGTT
     2281 CGCGACGCGA GGCTGGATGG CCTTCCCCAT TATGATTCTT CTCGCTTCCG GCGGCATCGG
     2341 GATGCCCGCG TTGCAGGCCA TGCTGTCCAG GCAGGTAGAT GACGACCATC AGGGACAGCT
     2401 TCAAGGATCG CTCGCGGCTC TTACCAGCCT AACTTCGATC ACTGGACCGC TGATCGTCAC
     2461 GGCGATTTAT GCCGCCTCGG CGAGCACATG GAACGGGTTG GCATGGATTG TAGGCGCCGC
     2521 CCTATACCTT GTCTGCCTCC CCGCGTTGCG TCGCGGTGCA TGGAGCCGGG CCACCTCGAC
     2581 CTGAATGGAA GCCGGCGGCA CCTCGCTAAC GGATTCACCA CTCCAAGAAT TGGAGCCAAT
     2641 CAATTCTTGC GGAGAACTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG
     2701 TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC CTGGCCACGG
     2761 GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG GCTGGCGGGG TTGCCTTACT
     2821 GGTTAGCAGA ATGAATCACC GATACGCGAG CGAACGTGAA GCGACTGCTG CTGCAAAACG
     2881 TCTGCGACCT GAGCAACAAC ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA
     2941 ACGCGGAAGT CCCCTACGTG CTGCTGAAGT TGCCCGCAAC AGAGAGTGGA ACCAACCGGT
     3001 GATACCACGA TACTATGACT GAGAGTCAAC GCCATGAGCG GCCTCATTTC TTATTCTGAG
     3061 TTACAACAGT CCGCACCGCT GTCCGGTAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC
     3121 GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG
     3181 CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCCCTGC
     3241 ACCATTATGT TCCGGATCTG CATCGCAGGA TGCTGCTGGC TACCCTGTGG AACACCTACA
     3301 TCTGTATTAA CGAAGCGCTA ACCGTTTTTA TCAGGCTCTG GGAGGCAGAA TAAATGATCA
     3361 TATCGTCAAT TATTACCTCC ACGGGGAGAG CCTGAGCAAA CTGGCCTCAG GCATTTGAGA
     3421 AGCACACGGT CACACTGCTT CCGGTAGTCA ATAAACCGGT AAACCAGCAA TAGACATAAG
     3481 CGGCTATTTA ACGACCCTGC CCTGAACCGA CGACCGGGTC GAATTTGCTT TCGAATTTCT
     3541 GCCATTCATC CGCTTATTAT CACTTATTCA GGCGTAGCAC CAGGCGTTTA AGGGCACCAA
     3601 TAACTGCCTT AAAAAAATTA CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA
     3661 TTAAGCATTC TGCCGACATG GAAGCCATCA CAGACGGCAT GATGAACCTG AATCGCCAGC
     3721 GGCATCAGCA CCTTGTCGCC TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG
     3781 AAGTTGTCCA TATTGGCCAC GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT
     3841 GAGACGAAAA ACATATTCTC AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA
     3901 CACGCCACAT CTTGCGAATA TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC
     3961 CAGAGCGATG AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA
     4021 TCCCATATCA CCAGCTCACC GTCTTTCATT GCCATACGGA ATTCCGGATG AGCATTCATC
     4081 AGGCGGGCAA GAATGTGAAT AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC
     4141 TTTAAAAAGG CCGTAATATC CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC
     4201 TGAAATGCCT CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA
     4261 GTGATTTTTT TCTCCATTTT AGCTTCCTTA GCTCCTGAAA ATCTCGATAA CTCAAAAAAT
     4321 ACGCCCGGTA GTGATCTTAT TTCATTATGG TGAAAGTTGG AACCTCTTAC GTGCCGATCA
     4381 ACGTCTCATT TTCGCCAAAA GTTGGCCCAG GGCTTCCCGG TATCAACAGG GACACCAGGA
     4441 TTTATTTATT CTGCGAAGTG ATCTTCCGTC ACAGGTATTT ATTCGGCGCA AAGTGCGTCG
     4501 GGTGATGCTG CCAACTTACT GATTTAGTGT ATGATGGTGT TTTTGAGGTG CTCCAGTGGC
     4561 TTCTGTTTCT ATCAGCTGTC CCTCCTGTTC AGCTACTGAC GGGGTGGTGC GTAACGGCAA
     4621 AAGCACCGCC GGACATCAGC GCTGGCGGAG TGTATACTGG CTTACTATGT TGGCACTGAT
     4681 GAGGGTGTCA GTGAAGTGCT TCATGTGGCA GGAGAAAAAA GGCTGCACCG GTGCGTCAGC
     4741 AGAATATGTG ATACAGGATA TATTCCGCTT CCTCGCTCAC TGACTCGCTA CGCTCGGTCG
     4801 TTCGACTGCG GCGAGCGGAA ATGGCTTACG AACGGGGCGG AGATTTCCTG GAAGATGCCA
     4861 GGAAGATACT TAACAGGGAA GTGAGAGGGC CGCGGCAAAG CCGTTTTTCC ATAGGCTCCG
     4921 CCCCCCTGAC AAGCATCACG AAATCTGACG CTCAAATCAG TGGTGGCGAA ACCCGACAGG
     4981 ACTATAAAGA TACCAGGCGT TTCCCCCTGG CGGCTCCCTC GTGCGCTCTC CTGTTCCTGC
     5041 CTTTCGGTTT ACCGGTGTCA TTCCGCTGTT ATGGCCGCGT TTGTCTCATT CCACGCCTGA
     5101 CACTCAGTTC CGGGTAGGCA GTTCGCTCCA AGCTGGACTG TATGCACGAA CCCCCCGTTC
     5161 AGTCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GAAAGACATG
     5221 CAAAAGCACC ACTGGCAGCA GCCACTGGTA ATTGATTTAG AGGAGTTAGT CTTGAAGTCA
     5281 TGCGCCGGTT AAGGCTAAAC TGAAAGGACA AGTTTTGGTG ACTGCGCTCC TCCAAGCCAG
     5341 TTACCTCGGT TCAAAGAGTT GGTAGCTCAG AGAACCTTCG AAAAACCGCC CTGCAAGGCG
     5401 GTTTTTTCGT TTTCAGAGCA AGAGATTACG CGCAGACCAA AACGATCTCA AGAAGATCAT
     5461 CTTATTAATC AGATAAAATA TTTCTAGATT TCAGTGCAAT TTATCTCTTC AAATGTAGCA
     5521 CCTGAAGTCA GCCCCATACG ATATAAGTTG TAATTCTCAT GTTTGACAGC TTATCAATCG
     5581 ATAAGAAACC AATTGTCCAT ATTGCATCAG
//
