LOCUS       550_C-BLA_1.2(pBAD322-K_D_C-BLA(OmpA)-sfGFP)_corrected      5522 bp    DNA     circular UNA 
DEFINITION  (KanR)
FEATURES             Location/Qualifiers
     Region          41..52
                     /note="Match: CGGCAGAAAAGT"
                     /note="Length of match: 12"
                     /note="Accuracy (%): 100"
                     /note="Description: 1540_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_O1_operator_region[REG]{araO1},12_bases,
     Region          83..96
                     /note="Match: TTGCTATGCCATAG"
                     /note="Length of match: 14"
                     /note="Accuracy (%): 100"
                     /note="Description: 1D6E_checksum."
                     /note="Found at strand: Positive"
                     /label=CAP_binding_site[OTH]{CAP_BS},14_bases,
     Region          128..156
                     /note="Match: CTGACGCTTTTTATCGCAACTCTCTACTG"
                     /note="Length of match: 29"
                     /note="Accuracy (%): 100"
                     /note="Description: 7B4_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_minimal_promoter[PRO]{ARA},29_bases,
     ORF             203..1495
                     /note="Length: 1266"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Region          203..268
                     /note="Match:
                     ATGAAAAAGACCGCGATTGCGATTGCGGTGGCGCTGGCGGGCTTTGCGACCGTGGCGC
                     AGGCGGCG"
                     /note="Length of match: 66"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=OmpA_SP
     Region          269..550
                     /note="Match:
                     CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTG
                     CAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGG
                     AGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
                     TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATA
                     GACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGACTAGTGGC"
                     /note="Length of match: 282"
                     /note="Accuracy (%): 100"
                     /note="Description: C-terminal BLA fragment"
                     /note="Found at strand: Positive"
                     /label=C-BLA
     important_prime 494..512
                     /note="Primer: CGAAATAGACAGATCGCTG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     550_C-BLA_1.2(pBAD322-K_D_C-BLA(OmpA)-sfGFP): 0"
                     /note="Primer binding region: 494..512"
                     /label="751_C-BLA_fwd (mismatches: 0)"
     Region          599..640
                     /note="Match: GTGATCATTCTGGCGGTGGGGCCCGGCCTGCTGCTCCTGGGG"
                     /note="Length of match: 42"
                     /note="Accuracy (%): 100"
                     /note="Found at strand: Positive"
                     /label=alphaVmut-TMD
     Region          640..1465
                     /note="Match:
                     GATCCACAAACTGGCAGTTAATGTTCAGCTGCCTGCCGAAGCAGCAGCGAAAGAAGCC
                     GCTGCCAAAGAAGCGGCAGCGAAAGAGGCTGCCGCGAAAGAGGCAGCAGCTAAAGCCG
                     CAAGCAAAGGTGAAGAACTGTTTACCGGTGTTGTTCCGATTCTGGTTGAACTGGATGG
                     TGATGTTAATGGCCACAAATTCTCAGTTCGTGGTGAAGGCGAAGGTGATGCAACCAAT
                     GGTAAACTGACCCTGAAATTTATCTGTACCACCGGCAAACTGCCGGTTCCGTGGCCGA
                     CCCTGGTTACCACCCTGACCTATGGTGTTCAGTGTTTCAGCCGTTATCCGGATCATAT
                     GAAACAGCACGATTTCTTCAAATCTGCAATGCCGGAAGGTTATGTTCAAGAACGTACC
                     ATCTCCTTTAAAGATGATGGCACCTATAAGACCCGTGCCGAAGTTAAATTTGAAGGTG
                     ATACCCTGGTGAATCGCATTGAACTGAAAGGCATCGATTTCAAAGAAGATGGTAATAT
                     CCTGGGCCATAAACTGGAATATAATTTCAACAGCCACAACGTGTATATCACCGCAGAT
                     AAACAGAAGAATGGCATCAAAGCCAACTTTAAGATCCGCCATAATGTTGAAGATGGCA
                     GCGTTCAGCTGGCAGATCATTATCAGCAGAATACACCGATTGGTGATGGTCCGGTTCT
                     GCTGCCGGATAATCATTATCTGAGCACCCAGAGCGTTCTGAGCAAAGATCCGAATGAG
                     AAACGTGATCACATGGTGCTGCTGGAATTTGTTACCGCAGCAGGTATTACCCATGGTA
                     TGGATGAACTGTAT"
                     /note="Length of match: 826"
                     /note="Accuracy (%): 100"
                     /note="Description: super folded GFP with linker region"
                     /note="Found at strand: Positive"
                     /label=sfGFP
     important_prime complement(931..950)
                     /note="Primer: gGGTCAGGGTGGTAACCAGG"
                     /note="Number of mismatches: 1"
                     /note="Number of other hits on
                     550_C-BLA_1.2(pBAD322-K_D_C-BLA(OmpA)-sfGFP): 0"
                     /note="Primer binding region: complement(931..950)"
                     /label="96_BFP_Y66H_rev (mismatches: 1)"
     Region          1469..1492
                     /note="Match: GGCGATTATAAAGATCACGACGGC"
                     /note="Length of match: 24"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=FLAG-Tag
     ORF             complement(1569..1895)
                     /note="Length: 327"
                     /note="Found at strand: negative"
                     /note="Start codon: ATG"
     Region          1608..1765
                     /note="Match:
                     TGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAA
                     ACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAG
                     GCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTT"
                     /note="Length of match: 158"
                     /note="Accuracy (%): 100"
                     /note="Description: D62_checksum."
                     /note="Found at strand: Positive"
                     /label=rrnB_transcriptional_termination_region[TER]{rrnB},158_bases,
     Region          1906..1933
                     /note="Match: AGAAGGCCATCCTGACGGATGGCCTTTT"
                     /note="Length of match: 28"
                     /note="Accuracy (%): 100"
                     /note="Description: 35_checksum."
                     /note="Found at strand: Positive"
                     /label=rrnB_T2_transcription_termination_sequence[TER]{rrnB_T2},28_bases,
     ORF             2112..2927
                     /note="Length: 816"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Region          2112..2927
                     /note="Match:
                     ATGAGCCATATTCAACGGGAAACGTCTTGCTCCAGGCCGCGATTAAATTCCAACATGG
                     ATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGAC
                     AATCTATCGTTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAA
                     GGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAAT
                     TTATGCCTCTTCCGACCATCAAGCATTTTATCCGGACTCCTGATGAAGCATGGTTACT
                     CACCACTGCGATCCCCGGCAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCA
                     GGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTG
                     TTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACG
                     AATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCT
                     GTTGAACAAGTCTGGAAAGAAATGCACAAACTTTTGCCATTCTCACCGGATTCAGTCG
                     TCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGG
                     TTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTA
                     TGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATG
                     GTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTT
                     CTAA"
                     /note="Length of match: 816"
                     /note="Accuracy (%): 100"
                     /note="Description: Kanamycin resistance"
                     /note="Found at strand: Positive"
                     /label=KanR
     Region          complement(4027..4218)
                     /note="Match:
                     GTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACAT
                     TAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGA
                     ATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGAT
                     GACGGTGAAAACCTCTGA"
                     /note="Length of match: 192"
                     /note="Accuracy (%): 100"
                     /note="Description: 1FC9_checksum."
                     /note="Found at strand: Negative"
                     /label=ROP_ORF[OTH]{ROP},192_bases,
     ORF             complement(4498..5427)
                     /note="Length: 930"
                     /note="Found at strand: negative"
                     /note="Start codon: ATG"
     Region          complement(4501..5427)
                     /note="Match:
                     ATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTG
                     AAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGG
                     TTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGA
                     ATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGG
                     GACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCA
                     TCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGT
                     CCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGT
                     TCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAT
                     TAACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAG
                     CAATTGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATA
                     ATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGA
                     TATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTC
                     CGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGG
                     CGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGG
                     TTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCG
                     AGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCA"
                     /note="Length of match: 927"
                     /note="Accuracy (%): 100"
                     /note="Found at strand: Negative"
                     /label=AraC
     Region          5406..5421
                     /note="Match: AAACCAATTGTCCATA"
                     /note="Length of match: 16"
                     /note="Accuracy (%): 100"
                     /note="Description: 2639_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_O2_operator_region[REG]{araO2},16_bases,
ORIGIN
        1 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG
       61 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG
      121 ATGCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG
      181 CTCTAGATAA CGAGGGCAAA AAATGAAAAA GACCGCGATT GCGATTGCGG TGGCGCTGGC
      241 GGGCTTTGCG ACCGTGGCGC AGGCGGCGCT ACTTACTCTA GCTTCCCGGC AACAATTAAT
      301 AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG
      361 CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC
      421 ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC
      481 AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG
      541 GACTAGTGGC AGCGGCGGCG GCAGCGGCGG CGGCAGCGGT TCTGCTAATC GAGCTAGCGT
      601 GATCATTCTG GCGGTGGGGC CCGGCCTGCT GCTCCTGGGG ATCCACAAAC TGGCAGTTAA
      661 TGTTCAGCTG CCTGCCGAAG CAGCAGCGAA AGAAGCCGCT GCCAAAGAAG CGGCAGCGAA
      721 AGAGGCTGCC GCGAAAGAGG CAGCAGCTAA AGCCGCAAGC AAAGGTGgAG AACTGTTTAC
      781 CGGTGTTGTT CCGATTCTGG TTGAACTGGA TGGTGATGTT AATGGCCACA AATTCTCAGT
      841 TCGTGGTGAA GGCGAAGGTG ATGCAACCAA TGGTAAACTG ACCCTGAAAT TTATCTGTAC
      901 CACCGGCAAA CTGCCGGTTC CGTGGCCGAC CCTGGTTACC ACCCTGACCT ATGGTGTTCA
      961 GTGTTTCAGC CGTTATCCGG ATCATATGAA ACAGCACGAT TTCTTCAAAT CTGCAATGCC
     1021 GGAAGGTTAT GTTCAAGAAC GTACCATCTC CTTTAAAGAT GATGGCACCT ATAAGACCCG
     1081 TGCCGAAGTT AAATTTGAAG GTGATACCCT GGTGAATCGC ATTGAACTGA AAGGCATCGA
     1141 TTTCAAAGAA GATGGTAATA TCCTGGGCCA TAAACTGGAA TATAATTTCA ACAGCCACAA
     1201 CGTGTATATC ACCGCAGATA AACAGAAGAA TGGCATCAAA GCCAACTTTA AGATCCGCCA
     1261 TAATGTTGAA GATGGCAGCG TTCAGCTGGC AGATCATTAT CAGCAGAATA CACCGATTGG
     1321 TGATGGTCCG GTTCTGCTGC CGGATAATCA TTATCTGAGC ACCCAGAGCG TTCTGAGCAA
     1381 AGATCCGAAT GAGAAACGTG ATCACATGGT GCTGCTGGAA TTTGTTACCG CAGCAGGTAT
     1441 TACCCATGGT ATGGATGAAC TGTATAAAGG CGATTATAAA GATCACGACG GCTAACTCGA
     1501 GGTCGACCTG CAGGCATGCA AGCTTGGCTG TTTTGGCGGA TGAGAGAAGA TTTTCAGCCT
     1561 GATACAGATT AAATCAGAAC GCAGAAGCGG TCTGATAAAA CAGAATTTGC CTGGCGGCAG
     1621 TAGCGCGGTG GTCCCACCTG ACCCCATGCC GAACTCAGAA GTGAAACGCC GTAGCGCCGA
     1681 TGGTAGTGTG GGGTCTCCCC ATGCGAGAGT AGGGAACTGC CAGGCATCAA ATAAAACGAA
     1741 AGGCTCAGTC GAAAGACTGG GCCTTTCGTT TTATCTGTTG TTTGTCGGTG AACGCTCTCC
     1801 TGAGTAGGAC AAATCCGCCG GGAGCGGATT TGAACGTTGC GAAGCAACGG CCCGGAGGGT
     1861 GGCGGGCAGG ACGCCCGCCA TAAACTGCCA GGCATCAAAT TAAGCAGAAG GCCATCCTGA
     1921 CGGATGGCCT TTTTGCGTTT CTACAAACTC TTTTGTTTAT TTTTCTAAAT ACATTCAAAT
     1981 ATGTATCCGC TCATGGAGCT CAAAGCCACG TTGTGTCTCA AAATCTCTGA TGTTACATTG
     2041 CACAAGATAA AAATATATCA TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA
     2101 CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCTTG CTCCAGGCCG CGATTAAATT
     2161 CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG
     2221 GTGCGACAAT CTATCGTTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG
     2281 GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG
     2341 AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGGAC TCCTGATGAA GCATGGTTAC
     2401 TCACCACTGC GATCCCCGGC AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG
     2461 GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT
     2521 GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA
     2581 ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC
     2641 AAGTCTGGAA AGAAATGCAC AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG
     2701 GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG
     2761 TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG
     2821 GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG
     2881 ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAAGAG CTCTCCATGA
     2941 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
     3001 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
     3061 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
     3121 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG
     3181 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
     3241 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
     3301 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
     3361 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC
     3421 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
     3481 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
     3541 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA
     3601 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
     3661 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG
     3721 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG
     3781 AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATGGT
     3841 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC
     3901 GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG
     3961 ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG
     4021 CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC
     4081 ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT
     4141 GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT
     4201 TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT
     4261 GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG
     4321 GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA
     4381 AATCACTCAG GGTCAATGCC AGCGCGATGC ATAATGTGCC TGTCAAATGG ACGAAGCAGG
     4441 GATTCTGCAA ACCCTATGCT ACTCCGTCAA GCCGTCAATT GTCTGATTCG TTACCAATTA
     4501 TGACAACTTG ACGGCTACAT CATTCACTTT TTCTTCACAA CCGGCACGGA ACTCGCTCGG
     4561 GCTGGCCCCG GTGCATTTTT TAAATACCCG CGAGAAATAG AGTTGATCGT CAAAACCAAC
     4621 ATTGCGACCG ACGGTGGCGA TAGGCATCCG GGTGGTGCTC AAAAGCAGCT TCGCCTGGCT
     4681 GATACGTTGG TCCTCGCGCC AGCTTAAGAC GCTAATCCCT AACTGCTGGC GGAAAAGATG
     4741 TGACAGACGC GACGGCGACA AGCAAACATG CTGTGCGACG CTGGCGATAT CAAAATTGCT
     4801 GTCTGCCAGG TGATCGCTGA TGTACTGACA AGCCTCGCGT ACCCGATTAT CCATCGGTGG
     4861 ATGGAGCGAC TCGTTAATCG CTTCCATGCG CCGCAGTAAC AATTGCTCAA GCAGATTTAT
     4921 CGCCAGCAGC TCCGAATAGC GCCCTTCCCC TTGCCCGGCG TTAATGATTT GCCCAAACAG
     4981 GTCGCTGAAA TGCGGCTGGT GCGCTTCATC CGGGCGAAAG AACCCCGTAT TGGCAAATAT
     5041 TGACGGCCAG TTAAGCCATT CATGCCAGTA GGCGCGCGGA CGAAAGTAAA CCCACTGGTG
     5101 ATACCATTCG CGAGCCTCCG GATGACGACC GTAGTGATGA ATCTCTCCTG GCGGGAACAG
     5161 CAAAATATCA CCCGGTCGGC AAACAAATTC TCGTCCCTGA TTTTTCACCA CCCCCTGACC
     5221 GCGAATGGTG AGATTGAGAA TATAACCTTT CATTCCCAGC GGTCGGTCGA TAAAAAAATC
     5281 GAGATAACCG TTGGCCTCAA TCGGCGTTAA ACCCGCCACC AGATGGGCAT TAAACGAGTA
     5341 TCCCGGCAGC AGGGGATCAT TTTGCGCTTC AGCCATACTT TTCATACTCC CGCCATTCAG
     5401 AGAAGAAACC AATTGTCCAT ATTGCATCAG ACATTGCCGT CACTGCGTCT TTTACTGGCT
     5461 CTTCTCGCTA ACCAAACCGG TAACCCCGCT TATTAAAAGC ATTCTGTAAC AAAGCGGGAC
     5521 CA
//
LOCUS       549_N-BLA_1.2(pToxRVII_N-BLA(OmpA)M180T-sfGFP)_corrected      5610 bp    DNA     circular UNA 
DEFINITION  araBAD-Promoter anti aIIb
FEATURES             Location/Qualifiers
     important_prime 69..88
                     /note="Primer: GCATTCTGTAACAAAGCGGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     549_N-BLA_1.2(pToxRVII_N-BLA(OmpA)M180T-sfGFP): 0"
                     /note="Primer binding region: 69..88"
                     /label="57_pToxRV_fwd(ToxSeqUp) (mismatches: 0)"
     Region          133..144
                     /note="Match: CGGCAGAAAAGT"
                     /note="Length of match: 12"
                     /note="Accuracy (%): 100"
                     /note="Description: 1540_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_O1_operator_region[REG]{araO1},12_bases,
     Region          175..188
                     /note="Match: TTGCTATGCCATAG"
                     /note="Length of match: 14"
                     /note="Accuracy (%): 100"
                     /note="Description: 1D6E_checksum."
                     /note="Found at strand: Positive"
                     /label=CAP_binding_site[OTH]{CAP_BS},14_bases,
     Region          220..248
                     /note="Match: CTGACGCTTTTTATCGCAACTCTCTACTG"
                     /note="Length of match: 29"
                     /note="Accuracy (%): 100"
                     /note="Description: 7B4_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_minimal_promoter[PRO]{ARA},29_bases,
     ORF             295..1821
                     /note="Length: 1527"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Region          295..360
                     /note="Match:
                     ATGAAAAAGACCGCGATTGCGATTGCGGTGGCGCTGGCGGGCTTTGCGACCGTGGCGC
                     AGGCGGCG"
                     /note="Length of match: 66"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=OmpA_SP
     important_prime complement(388..406)
                     /note="Primer: CACCCAACTGATCTTCAGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     549_N-BLA_1.2(pToxRVII_N-BLA(OmpA)M180T-sfGFP): 0"
                     /note="Primer binding region: complement(388..406)"
                     /label="752_N_BLA_col_rev (mismatches: 0)"
     important_prime 822..840
                     /note="Primer: CACGACGCCTGTAGCAATG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     549_N-BLA_1.2(pToxRVII_N-BLA(OmpA)M180T-sfGFP): 0"
                     /note="Primer binding region: 822..840"
                     /label="750_N-BLA_fwd (mismatches: 0)"
     Region          969..1794
                     /note="Match:
                     GATCCACAAACTGGCAGTTAATGTTCAGCTGCCTGCCGAAGCAGCAGCGAAAGAAGCC
                     GCTGCCAAAGAAGCGGCAGCGAAAGAGGCTGCCGCGAAAGAGGCAGCAGCTAAAGCCG
                     CAAGCAAAGGTGAAGAACTGTTTACCGGTGTTGTTCCGATTCTGGTTGAACTGGATGG
                     TGATGTTAATGGCCACAAATTCTCAGTTCGTGGTGAAGGCGAAGGTGATGCAACCAAT
                     GGTAAACTGACCCTGAAATTTATCTGTACCACCGGCAAACTGCCGGTTCCGTGGCCGA
                     CCCTGGTTACCACCCTGACCTATGGTGTTCAGTGTTTCAGCCGTTATCCGGATCATAT
                     GAAACAGCACGATTTCTTCAAATCTGCAATGCCGGAAGGTTATGTTCAAGAACGTACC
                     ATCTCCTTTAAAGATGATGGCACCTATAAGACCCGTGCCGAAGTTAAATTTGAAGGTG
                     ATACCCTGGTGAATCGCATTGAACTGAAAGGCATCGATTTCAAAGAAGATGGTAATAT
                     CCTGGGCCATAAACTGGAATATAATTTCAACAGCCACAACGTGTATATCACCGCAGAT
                     AAACAGAAGAATGGCATCAAAGCCAACTTTAAGATCCGCCATAATGTTGAAGATGGCA
                     GCGTTCAGCTGGCAGATCATTATCAGCAGAATACACCGATTGGTGATGGTCCGGTTCT
                     GCTGCCGGATAATCATTATCTGAGCACCCAGAGCGTTCTGAGCAAAGATCCGAATGAG
                     AAACGTGATCACATGGTGCTGCTGGAATTTGTTACCGCAGCAGGTATTACCCATGGTA
                     TGGATGAACTGTAT"
                     /note="Length of match: 826"
                     /note="Accuracy (%): 100"
                     /note="Description: super folded GFP with linker region"
                     /note="Found at strand: Positive"
                     /label=sfGFP
     important_prime complement(1260..1279)
                     /note="Primer: gGGTCAGGGTGGTAACCAGG"
                     /note="Number of mismatches: 1"
                     /note="Number of other hits on
                     549_N-BLA_1.2(pToxRVII_N-BLA(OmpA)M180T-sfGFP): 0"
                     /note="Primer binding region: complement(1260..1279)"
                     /label="96_BFP_Y66H_rev (mismatches: 1)"
     Region          1798..1821
                     /note="Match: GGCGATTATAAAGATCACGACGGC"
                     /note="Length of match: 24"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=FLAG-Tag
     Region          1920..1963
                     /note="Match:
                     ATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGA"
                     /note="Length of match: 44"
                     /note="Accuracy (%): 100"
                     /note="Description: F04_checksum."
                     /note="Found at strand: Positive"
                     /label=T7_terminator
     Region          1967..2581
                     /note="Match:
                     ATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTA
                     TCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGC
                     AGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGC
                     CTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTC
                     CCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGC
                     GCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATG
                     ATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGC
                     AGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCT
                     AACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACA
                     TGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGT
                     TGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACC"
                     /note="Length of match: 615"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=TetR_CDS
     ORF             3019..3318
                     /note="Length: 300"
                     /note="Found at strand: positive"
                     /note="Start codon: CTG"
     Region          complement(3621..4277)
                     /note="Match:
                     ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAG
                     AACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCT
                     GGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCC
                     TTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGA
                     AAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGA
                     GCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTT
                     CTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTA
                     AAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAG
                     TTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGC
                     AAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATG
                     CCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGA
                     TGAGTGGCAGGGCGGGGCG"
                     /note="Length of match: 657"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Negative"
                     /label=CAT_CDS
     Region          complement(4709..4909)
                     /note="Match:
                     GAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTT
                     CCAGGAAATCTCCGCCCCGTTCGTAAGCCATTTCCGCTCGCCGCAGTCGAACGACCGA
                     GCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCTGTATCACATATTCTGCTGA
                     CGCACCGGTGCAGCCTTTTTTCTCCTG"
                     /note="Length of match: 201"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Negative"
                     /label=p15a_ORI
     Region          5586..5601
                     /note="Match: AAACCAATTGTCCATA"
                     /note="Length of match: 16"
                     /note="Accuracy (%): 100"
                     /note="Description: 2639_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_O2_operator_region[REG]{araO2},16_bases,
ORIGIN
        1 ACATTGCCGT CACTGCGTCT TTTACTGGCT CTTCTCGCTA ACCAAACCGG TAACCCCGCT
       61 TATTAAAAGC ATTCTGTAAC AAAGCGGGAC CAAAGCCATG ACAAAAACGC GTAACAAAAG
      121 TGTCTATAAT CACGGCAGAA AAGTCCACAT TGATTATTTG CACGGCGTCA CACTTTGCTA
      181 TGCCATAGCA TTTTTATCCA TAAGATTAGC GGATGCTACC TGACGCTTTT TATCGCAACT
      241 CTCTACTGTT TCTCCATACC CGTTTTTTTG GGCTCTAGAT AACGAGGGCA AAAAATGAAA
      301 AAGACCGCGA TTGCGATTGC GGTGGCGCTG GCGGGCTTTG CGACCGTGGC GCAGGCGGCG
      361 CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC
      421 ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT
      481 CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC
      541 GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA
      601 CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC
      661 ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG
      721 GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA
      781 CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGACGCC TGTAGCAATG
      841 GCAACAACGT TGCGCAAACT ATTAACTGGC ACTAGTGGCA GCGGCGGCGG CAGCGGCGGC
      901 GGCAGCGGTT CTGCTAATCG AGCTAGCGTG ATCATTCTGG CGGTGGGGCC CGGCCTGCTG
      961 CTCCTGGGGA TCCACAAACT GGCAGTTAAT GTTCAGCTGC CTGCCGAAGC AGCAGCGAAA
     1021 GAAGCCGCTG CCAAAGAAGC GGCAGCGAAA GAGGCTGCCG CGAAAGAGGC AGCAGCTAAA
     1081 GCCGCAAGCA AAGGTGgAGA ACTGTTTACC GGTGTTGTTC CGATTCTGGT TGAACTGGAT
     1141 GGTGATGTTA ATGGCCACAA ATTCTCAGTT CGTGGTGAAG GCGAAGGTGA TGCAACCAAT
     1201 GGTAAACTGA CCCTGAAATT TATCTGTACC ACCGGCAAAC TGCCGGTTCC GTGGCCGACC
     1261 CTGGTTACCA CCCTGACCTA TGGTGTTCAG TGTTTCAGCC GTTATCCGGA TCATATGAAA
     1321 CAGCACGATT TCTTCAAATC TGCAATGCCG GAAGGTTATG TTCAAGAACG TACCATCTCC
     1381 TTTAAAGATG ATGGCACCTA TAAGACCCGT GCCGAAGTTA AATTTGAAGG TGATACCCTG
     1441 GTGAATCGCA TTGAACTGAA AGGCATCGAT TTCAAAGAAG ATGGTAATAT CCTGGGCCAT
     1501 AAACTGGAAT ATAATTTCAA CAGCCACAAC GTGTATATCA CCGCAGATAA ACAGAAGAAT
     1561 GGCATCAAAG CCAACTTTAA GATCCGCCAT AATGTTGAAG ATGGCAGCGT TCAGCTGGCA
     1621 GATCATTATC AGCAGAATAC ACCGATTGGT GATGGTCCGG TTCTGCTGCC GGATAATCAT
     1681 TATCTGAGCA CCCAGAGCGT TCTGAGCAAA GATCCGAATG AGAAACGTGA TCACATGGTG
     1741 CTGCTGGAAT TTGTTACCGC AGCAGGTATT ACCCATGGTA TGGATGAACT GTATAAAGGC
     1801 GATTATAAAG ATCACGACGG CTAACTCGAG CACCACCACC ACCACCACTG AGATCCGGCT
     1861 GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA
     1921 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGACCGATGC CCTTGAGAGC
     1981 CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTATCGTCG CCGCACTTAT
     2041 GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT GGGTCATTTT
     2101 CGGCGAGGAC CGCTTTCGCT GGAGCGCGAC GATGATCGGC CTGTCGCTTG CGGTATTCGG
     2161 AATCTTGCAC GCCCTCGCTC AAGCCTTCGT CACTGGTCCC GCCACCAAAC GTTTCGGCGA
     2221 GAAGCAGGCC ATTATCGCCG GCATGGCGGC CGACGCGCTG GGCTACGTCT TGCTGGCGTT
     2281 CGCGACGCGA GGCTGGATGG CCTTCCCCAT TATGATTCTT CTCGCTTCCG GCGGCATCGG
     2341 GATGCCCGCG TTGCAGGCCA TGCTGTCCAG GCAGGTAGAT GACGACCATC AGGGACAGCT
     2401 TCAAGGATCG CTCGCGGCTC TTACCAGCCT AACTTCGATC ACTGGACCGC TGATCGTCAC
     2461 GGCGATTTAT GCCGCCTCGG CGAGCACATG GAACGGGTTG GCATGGATTG TAGGCGCCGC
     2521 CCTATACCTT GTCTGCCTCC CCGCGTTGCG TCGCGGTGCA TGGAGCCGGG CCACCTCGAC
     2581 CTGAATGGAA GCCGGCGGCA CCTCGCTAAC GGATTCACCA CTCCAAGAAT TGGAGCCAAT
     2641 CAATTCTTGC GGAGAACTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG
     2701 TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC CTGGCCACGG
     2761 GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG GCTGGCGGGG TTGCCTTACT
     2821 GGTTAGCAGA ATGAATCACC GATACGCGAG CGAACGTGAA GCGACTGCTG CTGCAAAACG
     2881 TCTGCGACCT GAGCAACAAC ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA
     2941 ACGCGGAAGT CCCCTACGTG CTGCTGAAGT TGCCCGCAAC AGAGAGTGGA ACCAACCGGT
     3001 GATACCACGA TACTATGACT GAGAGTCAAC GCCATGAGCG GCCTCATTTC TTATTCTGAG
     3061 TTACAACAGT CCGCACCGCT GTCCGGTAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC
     3121 GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG
     3181 CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCCCTGC
     3241 ACCATTATGT TCCGGATCTG CATCGCAGGA TGCTGCTGGC TACCCTGTGG AACACCTACA
     3301 TCTGTATTAA CGAAGCGCTA ACCGTTTTTA TCAGGCTCTG GGAGGCAGAA TAAATGATCA
     3361 TATCGTCAAT TATTACCTCC ACGGGGAGAG CCTGAGCAAA CTGGCCTCAG GCATTTGAGA
     3421 AGCACACGGT CACACTGCTT CCGGTAGTCA ATAAACCGGT AAACCAGCAA TAGACATAAG
     3481 CGGCTATTTA ACGACCCTGC CCTGAACCGA CGACCGGGTC GAATTTGCTT TCGAATTTCT
     3541 GCCATTCATC CGCTTATTAT CACTTATTCA GGCGTAGCAC CAGGCGTTTA AGGGCACCAA
     3601 TAACTGCCTT AAAAAAATTA CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA
     3661 TTAAGCATTC TGCCGACATG GAAGCCATCA CAGACGGCAT GATGAACCTG AATCGCCAGC
     3721 GGCATCAGCA CCTTGTCGCC TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG
     3781 AAGTTGTCCA TATTGGCCAC GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT
     3841 GAGACGAAAA ACATATTCTC AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA
     3901 CACGCCACAT CTTGCGAATA TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC
     3961 CAGAGCGATG AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA
     4021 TCCCATATCA CCAGCTCACC GTCTTTCATT GCCATACGGA ATTCCGGATG AGCATTCATC
     4081 AGGCGGGCAA GAATGTGAAT AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC
     4141 TTTAAAAAGG CCGTAATATC CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC
     4201 TGAAATGCCT CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA
     4261 GTGATTTTTT TCTCCATTTT AGCTTCCTTA GCTCCTGAAA ATCTCGATAA CTCAAAAAAT
     4321 ACGCCCGGTA GTGATCTTAT TTCATTATGG TGAAAGTTGG AACCTCTTAC GTGCCGATCA
     4381 ACGTCTCATT TTCGCCAAAA GTTGGCCCAG GGCTTCCCGG TATCAACAGG GACACCAGGA
     4441 TTTATTTATT CTGCGAAGTG ATCTTCCGTC ACAGGTATTT ATTCGGCGCA AAGTGCGTCG
     4501 GGTGATGCTG CCAACTTACT GATTTAGTGT ATGATGGTGT TTTTGAGGTG CTCCAGTGGC
     4561 TTCTGTTTCT ATCAGCTGTC CCTCCTGTTC AGCTACTGAC GGGGTGGTGC GTAACGGCAA
     4621 AAGCACCGCC GGACATCAGC GCTGGCGGAG TGTATACTGG CTTACTATGT TGGCACTGAT
     4681 GAGGGTGTCA GTGAAGTGCT TCATGTGGCA GGAGAAAAAA GGCTGCACCG GTGCGTCAGC
     4741 AGAATATGTG ATACAGGATA TATTCCGCTT CCTCGCTCAC TGACTCGCTA CGCTCGGTCG
     4801 TTCGACTGCG GCGAGCGGAA ATGGCTTACG AACGGGGCGG AGATTTCCTG GAAGATGCCA
     4861 GGAAGATACT TAACAGGGAA GTGAGAGGGC CGCGGCAAAG CCGTTTTTCC ATAGGCTCCG
     4921 CCCCCCTGAC AAGCATCACG AAATCTGACG CTCAAATCAG TGGTGGCGAA ACCCGACAGG
     4981 ACTATAAAGA TACCAGGCGT TTCCCCCTGG CGGCTCCCTC GTGCGCTCTC CTGTTCCTGC
     5041 CTTTCGGTTT ACCGGTGTCA TTCCGCTGTT ATGGCCGCGT TTGTCTCATT CCACGCCTGA
     5101 CACTCAGTTC CGGGTAGGCA GTTCGCTCCA AGCTGGACTG TATGCACGAA CCCCCCGTTC
     5161 AGTCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GAAAGACATG
     5221 CAAAAGCACC ACTGGCAGCA GCCACTGGTA ATTGATTTAG AGGAGTTAGT CTTGAAGTCA
     5281 TGCGCCGGTT AAGGCTAAAC TGAAAGGACA AGTTTTGGTG ACTGCGCTCC TCCAAGCCAG
     5341 TTACCTCGGT TCAAAGAGTT GGTAGCTCAG AGAACCTTCG AAAAACCGCC CTGCAAGGCG
     5401 GTTTTTTCGT TTTCAGAGCA AGAGATTACG CGCAGACCAA AACGATCTCA AGAAGATCAT
     5461 CTTATTAATC AGATAAAATA TTTCTAGATT TCAGTGCAAT TTATCTCTTC AAATGTAGCA
     5521 CCTGAAGTCA GCCCCATACG ATATAAGTTG TAATTCTCAT GTTTGACAGC TTATCAATCG
     5581 ATAAGAAACC AATTGTCCAT ATTGCATCAG
//
LOCUS       480_N-BLA_1.1(pToxRVII_N-BLA(PelB_AN)M180T-sfGFP)_p15a_corrected      5607 bp    DNA     circular UNA 
DEFINITION  araBAD-Promoter anti aIIb
FEATURES             Location/Qualifiers
     Vector          join(1857..5607,1..141)
                     /origin="pToxRVII (5.952bp circular)Target vector: from
                     XhoI cut at 2201^2202 to MluI cut at 141^142,
                     3.892bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     Region          10..25
                     /note="Match: AAACCAATTGTCCATA"
                     /note="Length of match: 16"
                     /note="Accuracy (%): 100"
                     /note="Description: 2639_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_O2_operator
     Primer_binding_ 103..122
                     /note="Primer: GCATTCTGTAACAAAGCGGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 103..122"
                     /label="57pToxRV_fwd(ToxSeqUp) (mismatches: 0)"
     Insertion       142..1856
                     /origin="pToxRV_N-BLA(PelB)M180T-sfGFP (5.505bp
                     circular)Fragment: from MluI cut at 4744^4745 to XhoI cut
                     at 930^931, 1.691bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label=Insert
     Region          167..178
                     /note="Match: CGGCAGAAAAGT"
                     /note="Length of match: 12"
                     /note="Accuracy (%): 100"
                     /note="Description: 1540_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_O1_operator
     Promoter        194..203
                     /label="araBAD promoter region, cAMP-RP binding residues, (Nennenga 2011)"
     Region          209..222
                     /note="Match: TTGCTATGCCATAG"
                     /note="Length of match: 14"
                     /note="Accuracy (%): 100"
                     /note="Description: 1D6E_checksum."
                     /note="Found at strand: Positive"
                     /label=CAP_binding_site
     Primer_binding_ 214..233
                     /note="Primer: ATGCCATAGCATTTTTATCC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 214..233"
                     /label="primer_GATC-pBAD-FP (mismatches: 0)"
     Region          254..282
                     /note="Match: CTGACGCTTTTTATCGCAACTCTCTACTG"
                     /note="Length of match: 29"
                     /note="Accuracy (%): 100"
                     /note="Description: 7B4_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_promoter
     Primer_binding_ 304..331
                     /note="Primer: GGGCTCTAGATAACGAGGGCAAAAAATG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 304..331"
                     /label="101MT028 (mismatches: 0)"
     Primer_binding_ complement(304..361)
                     /note="Primer:
                     tcCaGCGGCtGCcgtagGCAaTaggTAtTtCATTTTTTGCCCTCGTTATCTAGAGCCC
                     "
                     /note="Number of mismatches: 15"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(304..361)"
                     /label="137pelBrev (mismatches: 15)"
     Primer_binding_ complement(304..347)
                     /note="Primer:
                     gatGgtGgTgATgATGCATTTTTTGCCCTCGTTATCTAGAGCCC"
                     /note="Number of mismatches: 8"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(304..347)"
                     /label="79MT023 (mismatches: 8)"
     Primer_binding_ complement(307..361)
                     /note="Primer:
                     taaacCaGCCagcgcCAGCcaaaTcTttTtCATTTTTTGCCCTCGTTATCTAGAG"
                     /note="Number of mismatches: 19"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(307..361)"
                     /label="292DsbASPrev (mismatches: 19)"
     Primer_binding_ complement(309..400)
                     /note="Primer:
                     gtgagCaaaaacAGgaaggcaaaatgcCGCaAaaaagGgaataagGGCgaCAcggAaa
                     tGTTgAatActCATTTTTTGCCCTCGTTATCTAG"
                     /note="Number of mismatches: 50"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(309..400)"
                     /label="438_SP-BLA_rev (mismatches: 50)"
     Primer_binding_ complement(309..394)
                     /note="Primer:
                     cGccgcctgcgcCACgGtcgcaaagcccgcCagcGCCaCcGCaatcgcaAtCgcggTc
                     TttTtCATTTTTTGCCCTCGTTATCTAG"
                     /note="Number of mismatches: 46"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(309..394)"
                     /label="439_OmpA_rev (mismatches: 46)"
     Primer_binding_ complement(309..397)
                     /note="Primer:
                     gTggGccatcGCcggCtggGCagcgaggAGcagCagacCaGCaGCaGCggtCgGCAGc
                     aggTAtTtCATTTTTTGCCCTCGTTATCTAG"
                     /note="Number of mismatches: 41"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(309..397)"
                     /label="437_PelB-v_rev (mismatches: 41)"
     Primer_binding_ complement(309..331)
                     /note="Primer: CATTTTTTGCCCTCGTTATCTAG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(309..331)"
                     /label="299BLA_delta_SP_rev (mismatches: 0)"
     Primer_binding_ 312..402
                     /note="Primer:
                     GATAACGAGGGCAAAAAATGCATTATAAACTGCTGTTTGCGGCCGCGGCCGCGAGCCT
                     GGCGAGCGCGGTGAGCGCTCACCCAGAAACGCT"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 312..402"
                     /label="42BLA_PelB_s (mismatches: 0)"
     Primer_binding_ complement(312..402)
                     /note="Primer:
                     AGCGTTTCTGGGTGAGCGCTCACCGCGCTCGCCAGGCTCGCGGCCGCGGCCGCAAACA
                     GCAGTTTATAATGCATTTTTTGCCCTCGTTATC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(312..402)"
                     /label="43BLA_PelB_as (mismatches: 0)"
     Region          329..388
                     /note="Match:
                     ATGCATTATAAACTGCTGTTTGCGGCCGCGGCCGCGAGCCTGGCGAGCGCGGTGAGCG
                     CT"
                     /note="Length of match: 60"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label="PelB signal peptide"
     ORF             329..1855
                     /note="Length: 1503"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Primer_binding_ complement(368..391)
                     /note="Primer: cgcAGCGCTCACCGCGCTCGCCAG"
                     /note="Number of mismatches: 3"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(368..391)"
                     /label="442_PelB-AN_H-A_rev (mismatches: 3)"
     Primer_binding_ complement(375..397)
                     /note="Primer: cagcaGGTGAGCGCTCACCGCGC"
                     /note="Number of mismatches: 5"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(375..397)"
                     /label="435_PelB+LL  (mismatches: 5)"
     Primer_binding_ complement(375..391)
                     /note="Primer: GTGAGCGCTCACCGCGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(375..391)"
                     /label="52BLA_PelB+H_rev (mismatches: 0)"
     Region          389..904
                     /note="Match:
                     ATGCATTATAAACTGCTGTTTGCGGCCGCGGCCGCGAGCCTGGCGAGCGCGGTGAGCG
                     CTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGT
                     GGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
                     GAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCC
                     GTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTT
                     GGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
                     TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAA
                     CGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAAC
                     TCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
                     ACCACGACGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCACTAG"
                     /note="Length of match: 578"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=N-BLa_M180T
     Primer_binding_ 392..412
                     /note="Primer: CCAGAAACGCTGGTGAAAGTA"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 392..412"
                     /label="298BLA_delta_SP_fwd (mismatches: 0)"
     Primer_binding_ 398..420
                     /note="Primer: ACGCTGGTGAAAGTAAAAGATGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 398..420"
                     /label="436_N-BLA_fwd  (mismatches: 0)"
     Primer_binding_ 838..879
                     /note="Primer: CGACGAGCGTGACACCACGACGCCTGTAGCAATGGCAACAAC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 838..879"
                     /label="44BLA_M180T_s (mismatches: 0)"
     Primer_binding_ complement(838..879)
                     /note="Primer: GTTGTTGCCATTGCTACAGGCGTCGTGGTGTCACGCTCGTCG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(838..879)"
                     /label="45BLA_M180T_as (mismatches: 0)"
     Primer_binding_ 878..958
                     /note="Primer:
                     ggtggcgGCAgtggcggtggTGGCAgcgGcGGCgGtGGTTCTGCTAATCGAGCTAGC"
                     /note="Number of mismatches: 23"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 878..934"
                     /label="49Ia5GP_Spacer15x_fwd (mismatches: 23)"
     Primer_binding_ complement(880..901)
                     /note="Primer: GCCAGTTAATAGTTTGCGCAAC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(880..901)"
                     /label="50BLA_194G_rev (mismatches: 0)"
     Primer_binding_ complement(894..949)
                     /note="Primer: gcTAcCgccACCGCTGCCACTAGTGCCAGTTA"
                     /note="Number of mismatches: 6"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(894..925)"
                     /label="199N-sBLA_Spacer_GGGS_rev (mismatches: 6)"
     Primer_binding_ complement(894..937)
                     /note="Primer: GCTGCCACTAGTGCCAGTTA"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(894..913)"
                     /label="208N-BLA_Spacer_rev (mismatches: 0)"
     Primer_binding_ complement(895..961)
                     /note="Primer:
                     gctGCcgcCgCcgcTgcCgccgCCGCTGCCACTAGTGCCAGTT"
                     /note="Number of mismatches: 16"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(895..937)"
                     /label="207N-BLA_2xGGGS_rev (mismatches: 16)"
     Primer_binding_ 902..958
                     /note="Primer: ggTgGcGGtAGCGGTTCTGCTAATCGAGCTAGC"
                     /note="Number of mismatches: 5"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 902..934"
                     /label="198sBLA_Spacer_GGGS_fwd (mismatches: 5)"
     Region          905..943
                     /label="GGS linker"
     Primer_binding_ 929..958
                     /note="Primer: gGTGGCgGtGGTTCTGCTAATCGAGCTAGC"
                     /note="Number of mismatches: 3"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 905..934"
                     /label="212BLA_Spacer_+3_fwd (mismatches: 3)"
     Primer_binding_ 932..958
                     /note="Primer: GGtgGCGGTTCTGCTAATCGAGCTAGC"
                     /note="Number of mismatches: 2"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 908..934"
                     /label="211BLA_Spacer_+2_fwd (mismatches: 2)"
     Primer_binding_ 935..958
                     /note="Primer: gGtGGTTCTGCTAATCGAGCTAGC"
                     /note="Number of mismatches: 2"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 911..934"
                     /label="210BLA_Spacer_+1_fwd (mismatches: 2)"
     Primer_binding_ 938..958
                     /note="Primer: GGTTCTGCTAATCGAGCTAGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 914..934"
                     /label="197sBLA_Spacer_fwd (mismatches: 0)"
     Primer_binding_ complement(947..976)
                     /note="Primer: gcCCagCgcAcacAcCACGCTAGCTCGATT"
                     /note="Number of mismatches: 10"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(923..952)"
                     /label="304P01909-0_Q5-rev_02 (mismatches: 10)"
     Region          959..997
                     /label="ApaI-containing TMD"
     Region-detail   959..1000
                     /note="Match: GTGATCATTCTGGCGGTGGGGCCCGGCCTGCTGCTCCTGGGG"
                     /note="Length of match: 42"
                     /note="Accuracy (%): 100"
                     /note="Found at strand: Positive"
                     /label=alphaVmut-TMD
     Primer_binding_ complement(983..1057)
                     /note="Primer:
                     atCcgggcTCGgcagcGgTTtcaCAtcgctCgGgcttTTtttaatCAGgcgGcGGATC
                     CCCAGGAGCAGCAGGCC"
                     /note="Number of mismatches: 36"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(959..1033)"
                     /label="258Cterm_GpA- (mismatches: 36)"
     Primer_binding_ 989..1016
                     /note="Primer: gcagcggcaGGGATCCACAAACTGGCAG"
                     /note="Number of mismatches: 9"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 965..992"
                     /label="129GpAelongate+3A+ (mismatches: 9)"
     Primer_binding_ complement(991..1067)
                     /note="Primer:
                     CccgttgAatatggctacTaGtKcYKcYKcYKcYkcykcykcykcYkcYkcYKcYkcy
                     gaTcaGGATCCCCAGGAGC"
                     /note="Number of mismatches: 45"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(967..1043)"
                     /label="33IaGP-NC_12x_as (mismatches: 45)"
     Primer_binding_ 992..1016
                     /note="Primer: gcggcaGGGATCCACAAACTGGCAG"
                     /note="Number of mismatches: 6"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 968..992"
                     /label="128GpAelongate+2A+ (mismatches: 6)"
     Primer_binding_ 995..1016
                     /note="Primer: gcaGGGATCCACAAACTGGCAG"
                     /note="Number of mismatches: 3"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 971..992"
                     /label="127GpAelongate+1A+ (mismatches: 3)"
     Region          1007..1117
                     /label="rigid linker"
     Primer_binding_ 1010..1030
                     /note="Primer: CTGGCAGTTAATGTTCAGCTG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 986..1006"
                     /label="300BLA_delta_TM_fwd (mismatches: 0)"
     Primer_binding_ complement(1010..1033)
                     /note="Primer: AGGCAGCTGAACATTAACTGCCAG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(986..1009)"
                     /label="266deltaLinkerrev (mismatches: 0)"
     Primer_binding_ complement(1016..1037)
                     /note="Primer: CGGCAGGCAGCTGAACATTAAC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(992..1013)"
                     /label="92sfGFP-linker_rev (mismatches: 0)"
     Primer_binding_ 1019..1051
                     /note="Primer: cgctggaAagTGaagGCCGAAGCAGCAGCGAAA"
                     /note="Number of mismatches: 12"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 995..1027"
                     /label="132QSOX2nativeC-term+ (mismatches: 12)"
     Primer_binding_ 1061..1142
                     /note="Primer:
                     aCCgAtGtgcCGctgagcAgcGtGGaaattGaaAAcccGGaAaCcagcgAtcagGCAA
                     GCAAAGGTGAAGAACTGTTTACCG"
                     /note="Number of mismatches: 36"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1037..1118"
                     /label="257Cterm_GpA+ (mismatches: 36)"
     Primer_binding_ complement(1093..1129)
                     /note="Primer: gctACtgaaatccaCGGCTTTAGCTGCTGCCTCTTTC"
                     /note="Number of mismatches: 12"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1069..1105)"
                     /label="131QSOX2nativeN-term- (mismatches: 12)"
     Primer_binding_ 1096..1139
                     /note="Primer:
                     tttaagAaggagatAtaCCatgAGCAAAGGTGAAGAACTGTTTA"
                     /note="Number of mismatches: 18"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1072..1115"
                     /label="318LIC16_GFP_fwd (mismatches: 18)"
     Primer_binding_ 1097..1142
                     /note="Primer:
                     tgGGCtagcaggAggatgGttAGCAAgGGcGAgGAgCTGTTTACCG"
                     /note="Number of mismatches: 20"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1073..1118"
                     /label="100MT027 (mismatches: 20)"
     Primer_binding_ 1106..1142
                     /note="Primer: agTcgtcatatgAGCAAAGGTGAAGAACTGTTTACCG"
                     /note="Number of mismatches: 11"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1082..1118"
                     /label="113MT033_fwd_sfGFP (mismatches: 11)"
     Primer_binding_ 1112..1142
                     /note="Primer: catatgAGCAAAGGTGAAGAACTGTTTACCG"
                     /note="Number of mismatches: 6"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1088..1118"
                     /label="106MT030_fwd_sfGFP (mismatches: 6)"
     Primer_binding_ 1115..1140
                     /note="Primer: GCAAGCAAAGGTGAAGAACTGTTTAC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1091..1116"
                     /label="265deltaLinkerfrw (mismatches: 0)"
     Region          1118..1828
                     /note="Match:
                     GATCCACAAACTGGCAGTTAATGTTCAGCTGCCTGCCGAAGCAGCAGCGAAAGAAGCC
                     GCTGCCAAAGAAGCGGCAGCGAAAGAGGCTGCCGCGAAAGAGGCAGCAGCTAAAGCCG
                     CAAGCAAAGGTGAAGAACTGTTTACCGGTGTTGTTCCGATTCTGGTTGAACTGGATGG
                     TGATGTTAATGGCCACAAATTCTCAGTTCGTGGTGAAGGCGAAGGTGATGCAACCAAT
                     GGTAAACTGACCCTGAAATTTATCTGTACCACCGGCAAACTGCCGGTTCCGTGGCCGA
                     CCCTGGTTACCACCCTGACCTATGGTGTTCAGTGTTTCAGCCGTTATCCGGATCATAT
                     GAAACAGCACGATTTCTTCAAATCTGCAATGCCGGAAGGTTATGTTCAAGAACGTACC
                     ATCTCCTTTAAAGATGATGGCACCTATAAGACCCGTGCCGAAGTTAAATTTGAAGGTG
                     ATACCCTGGTGAATCGCATTGAACTGAAAGGCATCGATTTCAAAGAAGATGGTAATAT
                     CCTGGGCCATAAACTGGAATATAATTTCAACAGCCACAACGTGTATATCACCGCAGAT
                     AAACAGAAGAATGGCATCAAAGCCAACTTTAAGATCCGCCATAATGTTGAAGATGGCA
                     GCGTTCAGCTGGCAGATCATTATCAGCAGAATACACCGATTGGTGATGGTCCGGTTCT
                     GCTGCCGGATAATCATTATCTGAGCACCCAGAGCGTTCTGAGCAAAGATCCGAATGAG
                     AAACGTGATCACATGGTGCTGCTGGAATTTGTTACCGCAGCAGGTATTACCCATGGTA
                     TGGATGAACTGTAT"
                     /note="Length of match: 826"
                     /note="Accuracy (%): 100"
                     /note="Description: super folded GFP with linker region"
                     /note="Found at strand: Positive"
                     /label=sfGFP
     Region-detail   1130..1132
                     /note="Match: GAAAGAGGCAGCAGCTAAAGCCGCAAGCAAAGGTGA"
                     /note="Length of match: 36"
                     /note="Accuracy (%): 100"
                     /note="Description: Glu->Gly PCR induced mut (SKGGEL not
                     SKGEEL), GFP function unaffected, Patrick linker cloning"
                     /note="Found at strand: Positive"
                     /label="Glu->Gly PCR induced mut (SKGGEL not SKGEEL), GFP function unaffected"
     Primer_binding_ 1135..1160
                     /note="Primer: GTTTACCGGTGTTGTTCCGATTCTGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1111..1136"
                     /label="201MT036_fwd_cfSGFP2 (mismatches: 0)"
     Primer_binding_ 1145..1178
                     /note="Primer: GTatgTggcAagCTctgcGtttctGATGGTGATG"
                     /note="Number of mismatches: 17"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1121..1154"
                     /label="166Q6ZRP7L10A+ (mismatches: 17)"
     Primer_binding_ complement(1291..1310)
                     /note="Primer: gGGTCAGGGTGGTAACCAGG"
                     /note="Number of mismatches: 1"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1267..1286)"
                     /label="96BFP_Y66H_rev (mismatches: 1)"
     Primer_binding_ 1298..1336
                     /note="Primer: ACCACCCTGACCTATGcTGTTCAGTGTTTCAGCCGTTAT"
                     /note="Number of mismatches: 1"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1274..1312"
                     /label="120sfGFPG67A+ (mismatches: 1)"
     Primer_binding_ complement(1298..1336)
                     /note="Primer: ATAACGGCTGAAACACTGAACAgCATAGGTCAGGGTGGT"
                     /note="Number of mismatches: 1"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1274..1312)"
                     /label="121sfGFPG67A- (mismatches: 1)"
     Primer_binding_ 1311..1331
                     /note="Primer: ATGGTGTTCAGTGTTTCAGCC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 1287..1307"
                     /label="95BFP_Y66H_fwd (mismatches: 0)"
     Primer_binding_ complement(1332..1358)
                     /note="Primer: CGTGCTGTTTCATgTGATCCGGATAAC"
                     /note="Number of mismatches: 1"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1308..1334)"
                     /label="111sfGFP_PDHMKQ_rev (mismatches: 1)"
     Primer_binding_ complement(1661..1711)
                     /note="Primer:
                     gTGATTATCCGGCAGCAGAACCGGACCATCACCAATCGGgGTATTCTGCTG"
                     /note="Number of mismatches: 2"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 1"
                     /note="Primer binding region: complement(1637..1687)"
                     /label="302cfSGFP2_fix_indel_rev (mismatches: 2)"
     Region          1832..1852
                     /note="Match: GGCGATTATAAAGATCACGACGGC"
                     /note="Length of match: 24"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=FLAG-Tag
     Primer_binding_ complement(1832..1881)
                     /note="Primer:
                     gcGgccgcGTcGactTaaaGCTtactagtGCCGTCGTGATCTTTATAATC"
                     /note="Number of mismatches: 21"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1808..1857)"
                     /label="107MT031_rev_sfGFP (mismatches: 21)"
     Primer_binding_ complement(1832..1852)
                     /note="Primer: GCCGTCGTGATCTTTATAATC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1808..1828)"
                     /label="97FLAG_rev (mismatches: 0)"
     Primer_binding_ complement(1862..1912)
                     /note="Primer:
                     gatCagcgGGttTaaacggGCcctCtagaCTCAcacGgccgGGTGGTGGTG"
                     /note="Number of mismatches: 29"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 1"
                     /note="Primer binding region: complement(1838..1888)"
                     /label="262QSOX2_pcDNA3.1rev (mismatches: 29)"
     Primer_binding_ complement(1868..1918)
                     /note="Primer:
                     gatcagcgggTTTaaacgggcccTctagaCtcacaCggccgGGTGGTGGTG"
                     /note="Number of mismatches: 35"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 1"
                     /note="Primer binding region: complement(1844..1894)"
                     /label="262QSOX2_pcDNA3.1rev (mismatches: 35)"
     Primer_binding_ complement(1932..1950)
                     /note="Primer: GCTAGTTATTGCTCAGCGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1908..1926)"
                     /label="primer_Seq-T7-Seqdn (mismatches: 0)"
     Primer_binding_ complement(1932..1949)
                     /note="Primer: CTAGTTATTGCTCAGCGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1908..1925)"
                     /label="primer_GATC-pET-RP (mismatches: 0)"
     Primer_binding_ complement(1934..1952)
                     /note="Primer: ATGCTAGTTATTGCTCAGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1910..1928)"
                     /label="primer_GATC-pRSET-RP (mismatches: 0)"
     Primer_binding_ complement(1935..1957)
                     /note="Primer: GGGTTATGCTAGTTATTGCTCAG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1911..1933)"
                     /label="primer_GATC-pET-24a (mismatches: 0)"
     Primer_binding_ complement(1935..1957)
                     /note="Primer: GGGTTATGCTAGTTATTGCTCAG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1911..1933)"
                     /label="110T7_terminator_rev (mismatches: 0)"
     Primer_binding_ complement(1939..1957)
                     /note="Primer: GGGTTATGCTAGTTATTGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1915..1933)"
                     /label="primer_GATC-pRSET-RPnew (mismatches: 0)"
     Region          1951..1994
                     /note="Match:
                     ATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGA"
                     /note="Length of match: 44"
                     /note="Accuracy (%): 100"
                     /note="Description: F04_checksum."
                     /note="Found at strand: Positive"
                     /label=T7_terminator
     Primer_binding_ complement(1965..1984)
                     /note="Primer: CCTCAAGACCCGTTTAGAGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1941..1960)"
                     /label="432_pT7_dNgoMIV_rev  (mismatches: 0)"
     Primer_binding_ complement(1993..2010)
                     /note="Primer: CcgTCAAGGGCATCGGTC"
                     /note="Number of mismatches: 2"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1969..1986)"
                     /label="primer_Seq-BiTermin800rev_Jana (mismatches: 2)"
     Region          1998..2612
                     /note="Match:
                     ATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTA
                     TCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGC
                     AGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGC
                     CTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTC
                     CCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGC
                     GCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATG
                     ATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGC
                     AGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCT
                     AACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACA
                     TGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGT
                     TGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACC"
                     /note="Length of match: 615"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label="TetR (apparrently deactivated by Lindner)"
     ORF             1998..2615
                     /note="Length: 618"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Primer_binding_ complement(2012..2029)
                     /note="Primer: GAGCTGACTGGGTTGAAG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(1988..2005)"
                     /label="primer_GATC-pGL3-1 (mismatches: 0)"
     Region          2854..3352
                     /label="p15a ori possible region"
     Primer_binding_ 3229..3247
                     /note="Primer: CACGGGGCCTGCCACCATA"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 3205..3223"
                     /label="431_pT7_dNgoMIV_fwd  (mismatches: 0)"
     ORF             complement(3649..4308)
                     /note="Length: 660"
                     /note="Found at strand: negative"
                     /note="Start codon: ATG"
     Region          complement(3652..4308)
                     /note="Match:
                     ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAG
                     AACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCT
                     GGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCC
                     TTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGA
                     AAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGA
                     GCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTT
                     CTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTA
                     AAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAG
                     TTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGC
                     AAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATG
                     CCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGA
                     TGAGTGGCAGGGCGGGGCG"
                     /note="Length of match: 657"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Negative"
                     /label=CAT(CmR)
     Primer_binding_ 3658..3673
                     /note="Primer: GCCCTGCCACTCATCG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 3634..3649"
                     /label="primer_GATC-pTeSp-2 (mismatches: 0)"
     Primer_binding_ complement(4768..4818)
                     /note="Primer:
                     gtgaTtAtccgGCagcagAaCcGgAccatcaCcaatcggggTATTCTGCTG"
                     /note="Number of mismatches: 32"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 1"
                     /note="Primer binding region: complement(4744..4794)"
                     /label="302cfSGFP2_fix_indel_rev (mismatches: 32)"
     Primer_binding_ 4854..4918
                     /note="Primer:
                     ccCgTtgaAtatGGctcataAcacCCcttgtatTaCggtaccGATACTTAACAGGGAA
                     GTGAGAG"
                     /note="Number of mismatches: 32"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 4830..4894"
                     /label="3p15a_fwd_KpnI_Ecol1 (mismatches: 32)"
     Primer_binding_ 4893..4918
                     /note="Primer: tcctAggCTTAACAGGGAAGTGAGAG"
                     /note="Number of mismatches: 6"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 4869..4894"
                     /label="9p15a_fwd_AvrII (mismatches: 6)"
     Primer_binding_ 5067..5084
                     /note="Primer: CCTGCCTTTCGGTTTACC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 5043..5060"
                     /label="5p15a_int_fwd (mismatches: 0)"
     Primer_binding_ complement(5367..5383)
                     /note="Primer: GAACCGAGGTAACTGGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(5343..5359)"
                     /label="6p15a_int_rev (mismatches: 0)"
     Primer_binding_ complement(5462..5520)
                     /note="Primer:
                     cagggGAtAacgcaggaaaGAacAtgtgagcGAatTcCTTGAGATCGTTTTGGTCTGC
                     G"
                     /note="Number of mismatches: 28"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(5438..5496)"
                     /label="4p15a_rev_EcoRI_Ecol1 (mismatches: 28)"
     Primer_binding_ complement(5464..5503)
                     /note="Primer: ggaATTccatAtggaATtccCTTGAGATCGTTTTGGTCTG"
                     /note="Number of mismatches: 14"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(5440..5479)"
                     /label="10p15a_rev_NdeI (mismatches: 14)"
     Primer_binding_ complement(5480..5504)
                     /note="Primer: TagaAaagATcAaAgGATCTTCTTG"
                     /note="Number of mismatches: 9"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(5456..5480)"
                     /label="268ColEI-pcDNArev (mismatches: 9)"
     Primer_binding_ complement(5510..5534)
                     /note="Primer: TAAATTGCACTGAAATCTAGAAATA"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: complement(5486..5510)"
                     /label="primer_GATC-TET-1_RP-1 (mismatches: 0)"
     Primer_binding_ 5544..5564
                     /note="Primer: ccGTAGCACCTGAAGTCAGCC"
                     /note="Number of mismatches: 2"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 5520..5540"
                     /label="primer_Seq-BiPromotor700fwd_Jana (mismatches: 2)"
     Primer_binding_ 5546..5566
                     /note="Primer: GTAGCACCTGAAGTCAGCCCC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     pToxRVII__N-BLA(PelB_AN)M180T-sfGFP: 0"
                     /note="Primer binding region: 5522..5542"
                     /label="primer_Seq-pEL_Isequ_Up (mismatches: 0)"
ORIGIN
        1 ATCGATAAGA AACCAATTGT CCATATTGCA TCAGACATTG CCGTCACTGC GTCTTTTACT
       61 GGCTCTTCTC GCTAACCAAA CCGGTAACCC CGCTTATTAA AAGCATTCTG TAACAAAGCG
      121 GGACCAAAGC CATGACAAAA ACGCGTAACA AAAGTGTCTA TAATCACGGC AGAAAAGTCC
      181 ACATTGATTA TTTGCACGGC GTCACACTTT GCTATGCCAT AGCATTTTTA TCCATAAGAT
      241 TAGCGGATGC TACCTGACGC TTTTTATCGC AACTCTCTAC TGTTTCTCCA TACCCGTTTT
      301 TTTGGGCTCT AGATAACGAG GGCAAAAAAT GCATTATAAA CTGCTGTTTG CGGCCGCGGC
      361 CGCGAGCCTG GCGAGCGCGG TGAGCGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC
      421 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT
      481 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT
      541 ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA
      601 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG
      661 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA
      721 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG
      781 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA
      841 CGAGCGTGAC ACCACGACGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG
      901 CACTAGTGGC AGCGGCGGCG GCAGCGGCGG CGGCAGCGGT TCTGCTAATC GAGCTAGCGT
      961 GATCATTCTG GCGGTGGGGC CCGGCCTGCT GCTCCTGGGG ATCCACAAAC TGGCAGTTAA
     1021 TGTTCAGCTG CCTGCCGAAG CAGCAGCGAA AGAAGCCGCT GCCAAAGAAG CGGCAGCGAA
     1081 AGAGGCTGCC GCGAAAGAGG CAGCAGCTAA AGCCGCAAGC AAAGGTGgAG AACTGTTTAC
     1141 CGGTGTTGTT CCGATTCTGG TTGAACTGGA TGGTGATGTT AATGGCCACA AATTCTCAGT
     1201 TCGTGGTGAA GGCGAAGGTG ATGCAACCAA TGGTAAACTG ACCCTGAAAT TTATCTGTAC
     1261 CACCGGCAAA CTGCCGGTTC CGTGGCCGAC CCTGGTTACC ACCCTGACCT ATGGTGTTCA
     1321 GTGTTTCAGC CGTTATCCGG ATCATATGAA ACAGCACGAT TTCTTCAAAT CTGCAATGCC
     1381 GGAAGGTTAT GTTCAAGAAC GTACCATCTC CTTTAAAGAT GATGGCACCT ATAAGACCCG
     1441 TGCCGAAGTT AAATTTGAAG GTGATACCCT GGTGAATCGC ATTGAACTGA AAGGCATCGA
     1501 TTTCAAAGAA GATGGTAATA TCCTGGGCCA TAAACTGGAA TATAATTTCA ACAGCCACAA
     1561 CGTGTATATC ACCGCAGATA AACAGAAGAA TGGCATCAAA GCCAACTTTA AGATCCGCCA
     1621 TAATGTTGAA GATGGCAGCG TTCAGCTGGC AGATCATTAT CAGCAGAATA CACCGATTGG
     1681 TGATGGTCCG GTTCTGCTGC CGGATAATCA TTATCTGAGC ACCCAGAGCG TTCTGAGCAA
     1741 AGATCCGAAT GAGAAACGTG ATCACATGGT GCTGCTGGAA TTTGTTACCG CAGCAGGTAT
     1801 TACCCATGGT ATGGATGAAC TGTATAAAGG CGATTATAAA GATCACGACG GCTAACTCGA
     1861 GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT
     1921 GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT
     1981 GAGGGGTTTT TTGACCGATG CCCTTGAGAG CCTTCAACCC AGTCAGCTCC TTCCGGTGGG
     2041 CGCGGGGCAT GACTATCGTC GCCGCACTTA TGACTGTCTT CTTTATCATG CAACTCGTAG
     2101 GACAGGTGCC GGCAGCGCTC TGGGTCATTT TCGGCGAGGA CCGCTTTCGC TGGAGCGCGA
     2161 CGATGATCGG CCTGTCGCTT GCGGTATTCG GAATCTTGCA CGCCCTCGCT CAAGCCTTCG
     2221 TCACTGGTCC CGCCACCAAA CGTTTCGGCG AGAAGCAGGC CATTATCGCC GGCATGGCGG
     2281 CCGACGCGCT GGGCTACGTC TTGCTGGCGT TCGCGACGCG AGGCTGGATG GCCTTCCCCA
     2341 TTATGATTCT TCTCGCTTCC GGCGGCATCG GGATGCCCGC GTTGCAGGCC ATGCTGTCCA
     2401 GGCAGGTAGA TGACGACCAT CAGGGACAGC TTCAAGGATC GCTCGCGGCT CTTACCAGCC
     2461 TAACTTCGAT CACTGGACCG CTGATCGTCA CGGCGATTTA TGCCGCCTCG GCGAGCACAT
     2521 GGAACGGGTT GGCATGGATT GTAGGCGCCG CCCTATACCT TGTCTGCCTC CCCGCGTTGC
     2581 GTCGCGGTGC ATGGAGCCGG GCCACCTCGA CCTGAATGGA AGCCGGCGGC ACCTCGCTAA
     2641 CGGATTCACC ACTCCAAGAA TTGGAGCCAA TCAATTCTTG CGGAGAACTG TGAATGCGCA
     2701 AACCAACCCT TGGCAGAACA TATCCATCGC GTCCGCCATC TCCAGCAGCC GCACGCGGCG
     2761 CATCTCGGGC AGCGTTGGGT CCTGGCCACG GGTGCGCATG ATCGTGCTCC TGTCGTTGAG
     2821 GACCCGGCTA GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC CGATACGCGA
     2881 GCGAACGTGA AGCGACTGCT GCTGCAAAAC GTCTGCGACC TGAGCAACAA CATGAATGGT
     2941 CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA AACGCGGAAG TCCCCTACGT GCTGCTGAAG
     3001 TTGCCCGCAA CAGAGAGTGG AACCAACCGG TGATACCACG ATACTATGAC TGAGAGTCAA
     3061 CGCCATGAGC GGCCTCATTT CTTATTCTGA GTTACAACAG TCCGCACCGC TGTCCGGTAG
     3121 CTCCTTCCGG TGGGCGCGGG GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT
     3181 CATGCAACTC GTAGGACAGG TGCCGGCAGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC
     3241 CACCATACCC ACGCCGAAAC AAGCGCCCTG CACCATTATG TTCCGGATCT GCATCGCAGG
     3301 ATGCTGCTGG CTACCCTGTG GAACACCTAC ATCTGTATTA ACGAAGCGCT AACCGTTTTT
     3361 ATCAGGCTCT GGGAGGCAGA ATAAATGATC ATATCGTCAA TTATTACCTC CACGGGGAGA
     3421 GCCTGAGCAA ACTGGCCTCA GGCATTTGAG AAGCACACGG TCACACTGCT TCCGGTAGTC
     3481 AATAAACCGG TAAACCAGCA ATAGACATAA GCGGCTATTT AACGACCCTG CCCTGAACCG
     3541 ACGACCGGGT CGAATTTGCT TTCGAATTTC TGCCATTCAT CCGCTTATTA TCACTTATTC
     3601 AGGCGTAGCA CCAGGCGTTT AAGGGCACCA ATAACTGCCT TAAAAAAATT ACGCCCCGCC
     3661 CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC
     3721 ACAGACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA
     3781 ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC
     3841 AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC
     3901 TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG
     3961 AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC
     4021 ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT
     4081 TGCCATACGG AATTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG
     4141 ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC
     4201 GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG
     4261 CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATTT TAGCTTCCTT
     4321 AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT AGTGATCTTA TTTCATTATG
     4381 GTGAAAGTTG GAACCTCTTA CGTGCCGATC AACGTCTCAT TTTCGCCAAA AGTTGGCCCA
     4441 GGGCTTCCCG GTATCAACAG GGACACCAGG ATTTATTTAT TCTGCGAAGT GATCTTCCGT
     4501 CACAGGTATT TATTCGGCGC AAAGTGCGTC GGGTGATGCT GCCAACTTAC TGATTTAGTG
     4561 TATGATGGTG TTTTTGAGGT GCTCCAGTGG CTTCTGTTTC TATCAGCTGT CCCTCCTGTT
     4621 CAGCTACTGA CGGGGTGGTG CGTAACGGCA AAAGCACCGC CGGACATCAG CGCTGGCGGA
     4681 GTGTATACTG GCTTACTATG TTGGCACTGA TGAGGGTGTC AGTGAAGTGC TTCATGTGGC
     4741 AGGAGAAAAA AGGCTGCACC GGTGCGTCAG CAGAATATGT GATACAGGAT ATATTCCGCT
     4801 TCCTCGCTCA CTGACTCGCT ACGCTCGGTC GTTCGACTGC GGCGAGCGGA AATGGCTTAC
     4861 GAACGGGGCG GAGATTTCCT GGAAGATGCC AGGAAGATAC TTAACAGGGA AGTGAGAGGG
     4921 CCGCGGCAAA GCCGTTTTTC CATAGGCTCC GCCCCCCTGA CAAGCATCAC GAAATCTGAC
     4981 GCTCAAATCA GTGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
     5041 GCGGCTCCCT CGTGCGCTCT CCTGTTCCTG CCTTTCGGTT TACCGGTGTC ATTCCGCTGT
     5101 TATGGCCGCG TTTGTCTCAT TCCACGCCTG ACACTCAGTT CCGGGTAGGC AGTTCGCTCC
     5161 AAGCTGGACT GTATGCACGA ACCCCCCGTT CAGTCCGACC GCTGCGCCTT ATCCGGTAAC
     5221 TATCGTCTTG AGTCCAACCC GGAAAGACAT GCAAAAGCAC CACTGGCAGC AGCCACTGGT
     5281 AATTGATTTA GAGGAGTTAG TCTTGAAGTC ATGCGCCGGT TAAGGCTAAA CTGAAAGGAC
     5341 AAGTTTTGGT GACTGCGCTC CTCCAAGCCA GTTACCTCGG TTCAAAGAGT TGGTAGCTCA
     5401 GAGAACCTTC GAAAAACCGC CCTGCAAGGC GGTTTTTTCG TTTTCAGAGC AAGAGATTAC
     5461 GCGCAGACCA AAACGATCTC AAGAAGATCA TCTTATTAAT CAGATAAAAT ATTTCTAGAT
     5521 TTCAGTGCAA TTTATCTCTT CAAATGTAGC ACCTGAAGTC AGCCCCATAC GATATAAGTT
     5581 GTAATTCTCA TGTTTGACAG CTTATCA
//
LOCUS       481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1_corrected      5519 bp    DNA     circular UNA 
DEFINITION  (KanR)
FEATURES             Location/Qualifiers
     Region          41..52
                     /note="Match: CGGCAGAAAAGT"
                     /note="Length of match: 12"
                     /note="Accuracy (%): 100"
                     /note="Description: 1540_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_O1_operator
     Promoter        68..77
                     /label="araBAD promoter region, cAMP-RP binding residues, (Nennenga 2011)"
     Region          83..96
                     /note="Match: TTGCTATGCCATAG"
                     /note="Length of match: 14"
                     /note="Accuracy (%): 100"
                     /note="Description: 1D6E_checksum."
                     /note="Found at strand: Positive"
                     /label=CAP_binding_site
     Region          128..156
                     /note="Match: CTGACGCTTTTTATCGCAACTCTCTACTG"
                     /note="Length of match: 29"
                     /note="Accuracy (%): 100"
                     /note="Description: 7B4_checksum."
                     /note="Found at strand: Positive"
                     /label=arabinose_promoter
     Primer_binding_ 178..205
                     /note="Primer: GGGCTCTAGATAACGAGGGCAAAAAATG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: 178..205"
                     /label="101_MT028 (mismatches: 0)"
     Primer_binding_ complement(183..205)
                     /note="Primer: CATTTTTTGCCCTCGTTATCTAG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: complement(183..205)"
                     /label="299_BLA_delta_SP_rev (mismatches: 0)"
     ORF             203..1492
                     /note="Length: 1266"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Region          203..262
                     /note="Match:
                     ATGCATTATAAACTGCTGTTTGCGGCCGCGGCCGCGAGCCTGGCGAGCGCGGTGAGCG
                     CT"
                     /note="Length of match: 60"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label="PelB signal peptide"
     Primer_binding_ complement(249..265)
                     /note="Primer: GTGAGCGCTCACCGCGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: complement(249..265)"
                     /label="52_BLA_PelB+H_rev (mismatches: 0)"
     Region          266..538
                     /note="Match:
                     CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTG
                     CAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGG
                     AGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
                     TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATA
                     GACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGACTAGTGGC"
                     /note="Length of match: 282"
                     /note="Accuracy (%): 100"
                     /note="Description: C-terminal BLA fragment"
                     /note="Found at strand: Positive"
                     /label=C-BLA
     Primer_binding_ 266..286
                     /note="Primer: CTACTTACTCTAGCTTCCCGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: 266..286"
                     /label="53_BLA_196L_fwd (mismatches: 0)"
     Primer_binding_ 491..509
                     /note="Primer: CGAAATAGACAGATCGCTG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: 491..509"
                     /label="751_C-BLA_fwd (mismatches: 0)"
     Primer_binding_ 506..526
                     /note="Primer: GCTGAGATAGGTGCCTCACTG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: 506..526"
                     /label="746_746_ampR_5-prime_forw (mismatches: 0)"
     Primer_binding_ complement(526..547)
                     /note="Primer: GCCACTAGTCCAATGCTTAATC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: complement(526..547)"
                     /label="48_BLA_Spacer_rev (mismatches: 0)"
     Primer_binding_ complement(532..550)
                     /note="Primer: GCTGCCACTAGTCCAATGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: complement(532..550)"
                     /label="209_C-BLA_Spacer_rev (mismatches: 0)"
     Primer_binding_ complement(532..574)
                     /note="Primer:
                     GCTGCCGCCGCCGCTGCCGCCGCCGCTGCCACTAGTCCAATGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: complement(532..574)"
                     /label="206_C-BLA_2xGGGS_rev (mismatches: 0)"
     Region          542..580
                     /label="GGS linker"
     Primer_binding_ 575..595
                     /note="Primer: GGTTCTGCTAATCGAGCTAGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: 575..595"
                     /label="197_sBLA_Spacer_fwd (mismatches: 0)"
     Primer_binding_ complement(575..595)
                     /note="Primer: GCTAGCTCGATTAGCAGAACC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: complement(575..595)"
                     /label="588_588_N-juxta_rev (mismatches: 0)"
     Region          596..634
                     /label="ApaI-containing TMD"
     Region-detail   596..637
                     /note="Match: GTGATCATTCTGGCGGTGGGGCCCGGCCTGCTGCTCCTGGGG"
                     /note="Length of match: 42"
                     /note="Accuracy (%): 100"
                     /note="Found at strand: Positive"
                     /label=alphaVmut-TMD
     Primer_binding_ 635..655
                     /note="Primer: GGGATCCACAAACTGGCAGTT"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: 635..655"
                     /label="590_590_C-juxta_fwd (mismatches: 0)"
     Region          644..754
                     /label="rigid linker"
     Primer_binding_ 647..667
                     /note="Primer: CTGGCAGTTAATGTTCAGCTG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: 647..667"
                     /label="300_BLA_delta_TM_fwd (mismatches: 0)"
     Primer_binding_ complement(647..670)
                     /note="Primer: AGGCAGCTGAACATTAACTGCCAG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: complement(647..670)"
                     /label="266_delta_Linker_rev (mismatches: 0)"
     Primer_binding_ complement(653..674)
                     /note="Primer: CGGCAGGCAGCTGAACATTAAC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: complement(653..674)"
                     /label="92_sfGFP-linker_rev (mismatches: 0)"
     Region-detail   730..765
                     /note="Match: GAAAGAGGCAGCAGCTAAAGCCGCAAGCAAAGGTGA"
                     /note="Length of match: 36"
                     /note="Accuracy (%): 100"
                     /note="Description: Glu->Gly PCR induced mut (SKGGEL not
                     SKGEEL), GFP function unaffected, Patrick linker cloning"
                     /note="Found at strand: Positive"
                     /label="Glu->Gly PCR induced mut (SKGGEL not SKGEEL), GFP function unaffected"
     Primer_binding_ 752..777
                     /note="Primer: GCAAGCAAAGGTGAAGAACTGTTTAC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: 752..777"
                     /label="265_delta_Linker_frw (mismatches: 0)"
     Region          755..1465
                     /note="Match:
                     GATCCACAAACTGGCAGTTAATGTTCAGCTGCCTGCCGAAGCAGCAGCGAAAGAAGCC
                     GCTGCCAAAGAAGCGGCAGCGAAAGAGGCTGCCGCGAAAGAGGCAGCAGCTAAAGCCG
                     CAAGCAAAGGTGAAGAACTGTTTACCGGTGTTGTTCCGATTCTGGTTGAACTGGATGG
                     TGATGTTAATGGCCACAAATTCTCAGTTCGTGGTGAAGGCGAAGGTGATGCAACCAAT
                     GGTAAACTGACCCTGAAATTTATCTGTACCACCGGCAAACTGCCGGTTCCGTGGCCGA
                     CCCTGGTTACCACCCTGACCTATGGTGTTCAGTGTTTCAGCCGTTATCCGGATCATAT
                     GAAACAGCACGATTTCTTCAAATCTGCAATGCCGGAAGGTTATGTTCAAGAACGTACC
                     ATCTCCTTTAAAGATGATGGCACCTATAAGACCCGTGCCGAAGTTAAATTTGAAGGTG
                     ATACCCTGGTGAATCGCATTGAACTGAAAGGCATCGATTTCAAAGAAGATGGTAATAT
                     CCTGGGCCATAAACTGGAATATAATTTCAACAGCCACAACGTGTATATCACCGCAGAT
                     AAACAGAAGAATGGCATCAAAGCCAACTTTAAGATCCGCCATAATGTTGAAGATGGCA
                     GCGTTCAGCTGGCAGATCATTATCAGCAGAATACACCGATTGGTGATGGTCCGGTTCT
                     GCTGCCGGATAATCATTATCTGAGCACCCAGAGCGTTCTGAGCAAAGATCCGAATGAG
                     AAACGTGATCACATGGTGCTGCTGGAATTTGTTACCGCAGCAGGTATTACCCATGGTA
                     TGGATGAACTGTAT"
                     /note="Length of match: 826"
                     /note="Accuracy (%): 100"
                     /note="Description: super folded GFP with linker region"
                     /note="Found at strand: Positive"
                     /label=sfGFP
     Primer_binding_ 772..797
                     /note="Primer: GTTTACCGGTGTTGTTCCGATTCTGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: 772..797"
                     /label="201_MT036_fwd_cfSGFP2 (mismatches: 0)"
     Primer_binding_ 948..968
                     /note="Primer: ATGGTGTTCAGTGTTTCAGCC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: 948..968"
                     /label="95_BFP_Y66H_fwd (mismatches: 0)"
     Region          1469..1489
                     /note="Match: GGCGATTATAAAGATCACGACGGC"
                     /note="Length of match: 24"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=FLAG-Tag
     Primer_binding_ complement(1469..1501)
                     /note="Primer: GACCTCGAGTTAGCCGTCGTGATCTTTATAATC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: complement(1469..1501)"
                     /label="703_703_FLAG_XhoI_rev (mismatches: 0)"
     Primer_binding_ complement(1469..1489)
                     /note="Primer: GCCGTCGTGATCTTTATAATC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: complement(1469..1489)"
                     /label="97_FLAG_rev (mismatches: 0)"
     ORF             complement(1566..1892)
                     /note="Length: 327"
                     /note="Found at strand: negative"
                     /note="Start codon: ATG"
     Region          1605..1762
                     /note="Match:
                     TGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAA
                     ACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAG
                     GCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTT"
                     /note="Length of match: 158"
                     /note="Accuracy (%): 100"
                     /note="Description: D62_checksum."
                     /note="Found at strand: Positive"
                     /label=rrnB_terminator
     Region          1903..1930
                     /note="Match: AGAAGGCCATCCTGACGGATGGCCTTTT"
                     /note="Length of match: 28"
                     /note="Accuracy (%): 100"
                     /note="Description: 35_checksum."
                     /note="Found at strand: Positive"
                     /label=rrnB_T2_terminator
     ORF             2109..2924
                     /note="Length: 816"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Region          2109..2924
                     /note="Match:
                     ATGAGCCATATTCAACGGGAAACGTCTTGCTCCAGGCCGCGATTAAATTCCAACATGG
                     ATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGAC
                     AATCTATCGTTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAA
                     GGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAAT
                     TTATGCCTCTTCCGACCATCAAGCATTTTATCCGGACTCCTGATGAAGCATGGTTACT
                     CACCACTGCGATCCCCGGCAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCA
                     GGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTG
                     TTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACG
                     AATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCT
                     GTTGAACAAGTCTGGAAAGAAATGCACAAACTTTTGCCATTCTCACCGGATTCAGTCG
                     TCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGG
                     TTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTA
                     TGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATG
                     GTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTT
                     CTAA"
                     /note="Length of match: 816"
                     /note="Accuracy (%): 100"
                     /note="Description: Kanamycin resistance"
                     /note="Found at strand: Positive"
                     /label=KanR
     Primer_binding_ 2864..2892
                     /note="Primer: TATTGATAATCCTGATATGAATAAATTGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: 2864..2892"
                     /label="749_749_kanR_3-prime_forw (mismatches: 0)"
     Primer_binding_ 2986..3010
                     /note="Primer: TAGAAAAGATCAAAGGATCTTCTTG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: 2986..3010"
                     /label="268_ColEI-pcDNA_rev (mismatches: 0)"
     Primer_binding_ complement(3296..3314)
                     /note="Primer: CTGCGCCTTATCCGGTAAC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: complement(3296..3314)"
                     /label="745_745_pBR322-ORI_reverse (mismatches: 0)"
     Primer_binding_ 3365..3387
                     /note="Primer: GACCTACACCGAACTGAGATACC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: 3365..3387"
                     /label="744_744_pBR322-ORI_forward (mismatches: 0)"
     Primer_binding_ complement(3604..3628)
                     /note="Primer: AGGAACCGTAAAAAGGCCGCGTTGC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: complement(3604..3628)"
                     /label="267_pcDNA-ColEI_frw (mismatches: 0)"
     Region          complement(4024..4248)
                     /label="pMB1 possible ori"
     Primer_binding_ complement(4384..4401)
                     /note="Primer: CGCTGGCATTGACCCTGA"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: complement(4384..4401)"
                     /label="77_pBAD322_rev (mismatches: 0)"
     Primer_binding_ 4402..4421
                     /note="Primer: CGATGCATAATGTGCCTGTC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: 4402..4421"
                     /label="76_pBAD322_fwd (mismatches: 0)"
     ORF             complement(4495..5424)
                     /note="Length: 930"
                     /note="Found at strand: negative"
                     /note="Start codon: ATG"
     Region          complement(4498..5424)
                     /note="Match:
                     ATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTG
                     AAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGG
                     TTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGA
                     ATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGG
                     GACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCA
                     TCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGT
                     CCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGT
                     TCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAT
                     TAACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAG
                     CAATTGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATA
                     ATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGA
                     TATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTC
                     CGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGG
                     CGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGG
                     TTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCG
                     AGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCA"
                     /note="Length of match: 927"
                     /note="Accuracy (%): 100"
                     /note="Found at strand: Negative"
                     /label=AraC
     Primer_binding_ 5496..5515
                     /note="Primer: GCATTCTGTAACAAAGCGGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on
                     481_C-BLA_1.1(pBAD322-K_D_C-BLA(PelB_AN)-sfGFP)_pMB1: 0"
                     /note="Primer binding region: 5496..5515"
                     /label="57_pToxRV_fwd(ToxSeqUp) (mismatches: 0)"
ORIGIN
        1 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG
       61 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG
      121 ATGCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG
      181 CTCTAGATAA CGAGGGCAAA AAATGCATTA TAAACTGCTG TTTGCGGCCG CGGCCGCGAG
      241 CCTGGCGAGC GCGGTGAGCG CTCACCTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA
      301 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG
      361 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT
      421 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC
      481 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGAC
      541 TAGTGGCAGC GGCGGCGGCA GCGGCGGCGG CAGCGGTTCT GCTAATCGAG CTAGCGTGAT
      601 CATTCTGGCG GTGGGGCCCG GCCTGCTGCT CCTGGGGATC CACAAACTGG CAGTTAATGT
      661 TCAGCTGCCT GCCGAAGCAG CAGCGAAAGA AGCCGCTGCC AAAGAAGCGG CAGCGAAAGA
      721 GGCTGCCGCG AAAGAGGCAG CAGCTAAAGC CGCAAGCAAA GGTGgAGAAC TGTTTACCGG
      781 TGTTGTTCCG ATTCTGGTTG AACTGGATGG TGATGTTAAT GGCCACAAAT TCTCAGTTCG
      841 TGGTGAAGGC GAAGGTGATG CAACCAATGG TAAACTGACC CTGAAATTTA TCTGTACCAC
      901 CGGCAAACTG CCGGTTCCGT GGCCGACCCT GGTTACCACC CTGACCTATG GTGTTCAGTG
      961 TTTCAGCCGT TATCCGGATC ATATGAAACA GCACGATTTC TTCAAATCTG CAATGCCGGA
     1021 AGGTTATGTT CAAGAACGTA CCATCTCCTT TAAAGATGAT GGCACCTATA AGACCCGTGC
     1081 CGAAGTTAAA TTTGAAGGTG ATACCCTGGT GAATCGCATT GAACTGAAAG GCATCGATTT
     1141 CAAAGAAGAT GGTAATATCC TGGGCCATAA ACTGGAATAT AATTTCAACA GCCACAACGT
     1201 GTATATCACC GCAGATAAAC AGAAGAATGG CATCAAAGCC AACTTTAAGA TCCGCCATAA
     1261 TGTTGAAGAT GGCAGCGTTC AGCTGGCAGA TCATTATCAG CAGAATACAC CGATTGGTGA
     1321 TGGTCCGGTT CTGCTGCCGG ATAATCATTA TCTGAGCACC CAGAGCGTTC TGAGCAAAGA
     1381 TCCGAATGAG AAACGTGATC ACATGGTGCT GCTGGAATTT GTTACCGCAG CAGGTATTAC
     1441 CCATGGTATG GATGAACTGT ATAAAGGCGA TTATAAAGAT CACGACGGCT AACTCGAGGT
     1501 CGACCTGCAG GCATGCAAGC TTGGCTGTTT TGGCGGATGA GAGAAGATTT TCAGCCTGAT
     1561 ACAGATTAAA TCAGAACGCA GAAGCGGTCT GATAAAACAG AATTTGCCTG GCGGCAGTAG
     1621 CGCGGTGGTC CCACCTGACC CCATGCCGAA CTCAGAAGTG AAACGCCGTA GCGCCGATGG
     1681 TAGTGTGGGG TCTCCCCATG CGAGAGTAGG GAACTGCCAG GCATCAAATA AAACGAAAGG
     1741 CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA
     1801 GTAGGACAAA TCCGCCGGGA GCGGATTTGA ACGTTGCGAA GCAACGGCCC GGAGGGTGGC
     1861 GGGCAGGACG CCCGCCATAA ACTGCCAGGC ATCAAATTAA GCAGAAGGCC ATCCTGACGG
     1921 ATGGCCTTTT TGCGTTTCTA CAAACTCTTT TGTTTATTTT TCTAAATACA TTCAAATATG
     1981 TATCCGCTCA TGGAGCTCAA AGCCACGTTG TGTCTCAAAA TCTCTGATGT TACATTGCAC
     2041 AAGATAAAAA TATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA
     2101 GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCTTGCTC CAGGCCGCGA TTAAATTCCA
     2161 ACATGGATGC TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG
     2221 CGACAATCTA TCGTTTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA
     2281 AAGGTAGCGT TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT
     2341 TTATGCCTCT TCCGACCATC AAGCATTTTA TCCGGACTCC TGATGAAGCA TGGTTACTCA
     2401 CCACTGCGAT CCCCGGCAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG
     2461 AAAATATTGT TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA
     2521 ATTGTCCTTT TAACAGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA
     2581 ACGGTTTGGT TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG
     2641 TCTGGAAAGA AATGCACAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG
     2701 ATTTCTCACT TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG
     2761 GACGAGTCGG AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG
     2821 AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA
     2881 TGAATAAATT GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAAGAGCTC TCCATGACCA
     2941 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG
     3001 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC
     3061 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA
     3121 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC
     3181 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG
     3241 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC
     3301 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC
     3361 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC
     3421 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA
     3481 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC
     3541 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG
     3601 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT
     3661 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA
     3721 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC
     3781 GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATGGTGCA
     3841 CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT
     3901 ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG
     3961 GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT
     4021 GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC
     4081 AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG
     4141 TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT
     4201 TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT
     4261 ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT
     4321 ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT
     4381 CACTCAGGGT CAATGCCAGC GCGATGCATA ATGTGCCTGT CAAATGGACG AAGCAGGGAT
     4441 TCTGCAAACC CTATGCTACT CCGTCAAGCC GTCAATTGTC TGATTCGTTA CCAATTATGA
     4501 CAACTTGACG GCTACATCAT TCACTTTTTC TTCACAACCG GCACGGAACT CGCTCGGGCT
     4561 GGCCCCGGTG CATTTTTTAA ATACCCGCGA GAAATAGAGT TGATCGTCAA AACCAACATT
     4621 GCGACCGACG GTGGCGATAG GCATCCGGGT GGTGCTCAAA AGCAGCTTCG CCTGGCTGAT
     4681 ACGTTGGTCC TCGCGCCAGC TTAAGACGCT AATCCCTAAC TGCTGGCGGA AAAGATGTGA
     4741 CAGACGCGAC GGCGACAAGC AAACATGCTG TGCGACGCTG GCGATATCAA AATTGCTGTC
     4801 TGCCAGGTGA TCGCTGATGT ACTGACAAGC CTCGCGTACC CGATTATCCA TCGGTGGATG
     4861 GAGCGACTCG TTAATCGCTT CCATGCGCCG CAGTAACAAT TGCTCAAGCA GATTTATCGC
     4921 CAGCAGCTCC GAATAGCGCC CTTCCCCTTG CCCGGCGTTA ATGATTTGCC CAAACAGGTC
     4981 GCTGAAATGC GGCTGGTGCG CTTCATCCGG GCGAAAGAAC CCCGTATTGG CAAATATTGA
     5041 CGGCCAGTTA AGCCATTCAT GCCAGTAGGC GCGCGGACGA AAGTAAACCC ACTGGTGATA
     5101 CCATTCGCGA GCCTCCGGAT GACGACCGTA GTGATGAATC TCTCCTGGCG GGAACAGCAA
     5161 AATATCACCC GGTCGGCAAA CAAATTCTCG TCCCTGATTT TTCACCACCC CCTGACCGCG
     5221 AATGGTGAGA TTGAGAATAT AACCTTTCAT TCCCAGCGGT CGGTCGATAA AAAAATCGAG
     5281 ATAACCGTTG GCCTCAATCG GCGTTAAACC CGCCACCAGA TGGGCATTAA ACGAGTATCC
     5341 CGGCAGCAGG GGATCATTTT GCGCTTCAGC CATACTTTTC ATACTCCCGC CATTCAGAGA
     5401 AGAAACCAAT TGTCCATATT GCATCAGACA TTGCCGTCAC TGCGTCTTTT ACTGGCTCTT
     5461 CTCGCTAACC AAACCGGTAA CCCCGCTTAT TAAAAGCATT CTGTAACAAA GCGGGACCA
//
