LOCUS       pFS461.xdna    6683 bp      DNA     circular   2/16/2017
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: . - [LOCUS]: pFS181 - [ACCESSION]: 
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    307..1422
                     /label=leu1
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2228..2980
                     /label=adh1 Pr
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3007..4404
                     /label=Z3EV
                     /SerialCloner_Color=&h80FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4421..4440
                     /label=T3 r primer
                     /SerialCloner_Color=&h80FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4462..4478
                     /label=M13 r primer
                     /SerialCloner_Color=&h80FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    29..51
                     /label=3GEX r primer
                     /SerialCloner_Color=&h80FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 
      181 ACCATACGAT ATCCCAATCT GTAGTATAAA AAGTGCAACA GTGTTGAGTT TCCCGAAACC 
      241 AGGCATTTTT GCGGTAGGGT CTGAAACTTT CACGAAACCA TTTCACAACA AAACTTCTTC 
      301 AACAACATGT GTGCAAAGAA AATCGTCGTC TTACCAGGAG ACCATATTGG CCCTGAAATT 
      361 GTTGCTTCTG CCTTGGAGGT TTTGAAAGTC GTTGAGAAGA AGCGACCTGA GTTAAAACTC 
      421 GAGTTTGAAG AACACAAGAT TGGAGGTGCC TCTATTGATG CCTATGGAAC CCCTTTGACT 
      481 GACGAGACTG TGAAGGCTTG TTTGGAAGCT GACGGTGTTC TTTTGGGTGC CGTTGGTGGT 
      541 CCTGAATGGA CCAACCCCAA TTGTCGTCCT GAGCAAGGTT TATTGAAGCT TCGTAAGAGT 
      601 ATGGGTGTTT GGGCCAACCT TCGACCTTGC AACTTTGCCA GCAAGTCTTT AGTCAAGTAC 
      661 AGCCCTTTGA AGCCTGAAAT CGTTGAAGGT GTCGATTTTT GTGTTGTACG AGAACTTACT 
      721 GGAGGTTGTT ACTTTGGTGA GCGCACTGAG GACAACGGAT CGGGTTATGC TATGGACACT 
      781 TGGCCTTACA GTTTGGAAGA AGTTTCTCGT ATTGCTCGTT TGGCTGCTTG GTTAGCTGAA 
      841 ACTTCCAACC CTCCTGCTCC CGTCACATTA CTCGACAAAG CTAATGTTTT GGCAACTTCT 
      901 CGTCTTTGGC GCAAGACTGT AGCTAAGATT TTTAAGGAGG AATATCCTCA CCTTACCTTA 
      961 AAAAACCAGC TCATTGACTC CGCAGCCATG CTTTTGGTCA AGAGCCCTCG TACACTTAAC 
     1021 GGTGTTGTTT TGACTGACAA CTTGTTTGGT GACATTATTT CAGATGAGGC TTCTGTCATT 
     1081 CCTGGTAGCT TGGGCCTTTT GCCTTCTGCC TCCCTTTCCG GTGTGGTAGG AAAATCAGAA 
     1141 GAAAAGGTTC ATTGTTTGGT TGAGCCCATT CACGGTAGCG CTCCCGATAT CGCTGGTAAG 
     1201 GGCATTGTTA ATCCTGTTGG TACAATTTTA TCTGCTTCCC TTCTCCTTCG TTATGGTTTG 
     1261 AATGCTCCTA AGGAGGCTGA AGCTATCGAA GCCGCCGTAC GCAAGGTCTT GGATGATACT 
     1321 TCAATTGGTG GACGTGGTCT TTATACTCGC GATTTGGGAG GTGAGGCTTC TACCGCTGAT 
     1381 ATTACTAAGG CTGTTGTTGA AGAACTTGAA AAAATTTTGT AATAGGGTTT AATGTAGAAT 
     1441 AAATTCATAT GATTTGATTT GACCTAATTT TTATTGATTA ATTGTGTCGC ATATTTATTA 
     1501 TCGTTAAAAA TGCAATTGAC AAAAGCGTAA ATTTTAAGGC TCTAATGTTT TTTCTTTTGG 
     1561 TATGTTATAG TCAAGCAAAG ATGTATTAAG TTGCAAAAGC ATGGAATTGA TTTACGACAT 
     1621 CCGTTCAACT TTATGACCGA GTTGTCGCTG TGTACTTACT CTTTTTTTAC TATGAAAAGT 
     1681 TTCAAACATA ATATCATTAA TTATTTCTTT TTAAACGAAG TAACCTGTAA CATAAATAAT 
     1741 AGGGGAAAAG AAGAAAAAAA CCATAAAAAT TCGAGAATCG TAACAATTAT TATTGTAACG 
     1801 ATGACAACCC GATTCTTTGA GAGAGCCAGA GACATGACTT TTGCATTACC GTAATTGGTT 
     1861 TCTACTTATG AATTGTAAAC TTGTGTAGGG AATTTCATCC ACCAGCTATC GCGGGCTGCT 
     1921 CTCTGCATCA GTGTTAGCAC ATTTTATATT AATGAAGATT TTGCTATGCA AAATACATTG 
     1981 ACAAGGAGGA GGGCACCACA CAAAAAGTTA GGTGTAACAG AAAATCATGA AACTATGATT 
     2041 CCTAATTTAT ATATTGGAGG ATTTTCTCTA AAAAAAAAAA AATACAACAA ATAAAAAACA 
     2101 CTCAATGACC TGACCATTTG ATGGAGTTTA AGTCAATACC TTCTTGAACC ATTTCCCATA 
     2161 ATGGTGAAAG TTCCCTCAAG AATTTTACTC TGTCAGAAAC GGCCTTAACG ACGTAGTCGA 
     2221 CAAGCTtgCA TGCCCTACAA CAACTAAGAA AATGGCTATC ATGCGGAAGC TGGTGAGAAG 
     2281 AACAGCATCG GGACAAGGGA AGGAAGAACA AAGACAAAGA AAACAAAAGA AAGCAATTGA 
     2341 AAACAAAACA AAACAATTTT CATTCCTTCT CTTATCATTC CTTTTCTTTT CTTTTCTCTC 
     2401 ATTCAACGCA CTCCATCGTA TCCGTATTCC TCTTATTTTT TCTCTTTCTC TATATCCATT 
     2461 TCTTTCTCTC TAGGTGTGTC CTCTCTCTCT CTTCAATTTC TCTACTCCGC ATTCCAACGC 
     2521 ATCCTTCCCC CAACCTCCCA TTTCCTCCTT ACGGCCCGAT AGCGATCGTC TTTCCCTCGC 
     2581 TATCACTCGC TACCGGCCCC TCCTCTGCAC CGTAACCTCC TACGTATTTA CCATATCATA 
     2641 AAGTTTTTTC CGACGCTTAT CGCTGACCCC CTGTCGCCCT CCTATTGGCT TCCGGATTAT 
     2701 CTTCTTGTCC ATAAGGTGAT CCATGCTTCC TGAAGATTCC CGAAATGTGT CCACTTTGGC 
     2761 GGGGAATCAT TCCATCCACT TCTTTCTCTC TCGCTTTCCT CATTCGGCGC TCCCCTTCCG 
     2821 CGTCTCATTG GTCTTCCGCT CCGTTTTTGC TTTGCCGATG TTACTTGGGG AGAGGTGCGA 
     2881 TAATCCTTTC GCAAAAACTC GGTTTGACGC CTCCCATGGT ATAAATAGTG GGTGGTGGAC 
     2941 AGGTGCCTTC GCTTTTCTTT TCTTTAAGCA AGAGAATTcC tgcaggtcga ctctagagGA 
     3001 TCTCATatgg gtacccgccc atatgcttgc cctgtcgagt cctgcgatcg ccgcttttct 
     3061 cgctcggatg agcttacccg ccatatccgc atccataccg gtcagaagcc cttccagtgt 
     3121 cgaatctgca tgcgtaactt cagtcgtagt gaccacctta ccacccacat ccgcacccac 
     3181 acaggcgaga agccttttgc ctgtgacatt tgtgggagga agtttgccag gagtgatgaa 
     3241 cgcaagaggc ataccaaaat ccatacaggt ggcggaggca cacctgcagc tgcgtcgact 
     3301 ctagaggatc catctgctgg agacatgaga gctgccaacc tttggccaag cccgctcatg 
     3361 atcaaacgct ctaagaagaa cagcctggcc ttgtccctga cggccgacca gatggtcagt 
     3421 gccttgttgg atgctgagcc ccccatactc tattccgagt atgatcctac cagacccttc 
     3481 agtgaagctt cgatgatggg cttactgacc aacctggcag acagggagct ggttcacatg 
     3541 atcaactggg cgaagagggt gccaggcttt gtggatttga ccctccatga tcaggtccac 
     3601 cttctagaat gtgcctggct agagatcctg atgattggtc tcgtctggcg ctccatggag 
     3661 cacccagtga agctactgtt tgctcctaac ttgctcttgg acaggaacca gggaaaatgt 
     3721 gtagagggca tggtggagat cttcgacatg ctgctggcta catcatctcg gttccgcatg 
     3781 atgaatctgc agggagagga gtttgtgtgc ctcaaatcta ttattttgct taattctgga 
     3841 gtgtacacat ttctgtccag caccctgaag tctctggaag agaaggacca tatccaccga 
     3901 gtcctggaca agatcacaga cactttgatc cacctgatgg ccaaggcagg cctgaccctg 
     3961 cagcagcagc accagcggct ggcccagctc ctcctcatcc tctcccacat caggcacatg 
     4021 agtaacaaag gcatggagca tctgtacagc atgaagtgca agaacgtggt gcccctctat 
     4081 gacctgctgc tggagatgct ggacgcccac cgcctacatg cgcccactag ccgtggaggg 
     4141 gcatccgtgg aggagacgga ccaaagccac ttggccactg cgggctctac ttcatcggag 
     4201 ctccacttag acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat 
     4261 ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc 
     4321 ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt 
     4381 ggaattgacg agtacggtgg gtagGAGCTC CAGCTTTTGT TCCCTTTAGT GAGGGTTAAT 
     4441 TGCGCGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC 
     4501 AATTCCACAC AACATAGGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT 
     4561 GAGGTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC 
     4621 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 
     4681 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 
     4741 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4801 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4861 GTTTTTCCAT AGGCTCGGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4921 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTC CCCCCTGGAA GCTCCCTCGT 
     4981 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5041 AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5101 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5161 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5221 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5281 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5341 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5401 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5461 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5521 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5581 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 
     5641 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTGCCCGT 
     5701 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 
     5761 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 
     5821 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 
     5881 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 
     5941 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 
     6001 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGAAAAAAA GCGGTTAGCT CCTTCGGTCC 
     6061 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 
     6121 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 
     6181 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 
     6241 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 
     6301 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 
     6361 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 
     6421 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 
     6481 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 
     6541 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 
     6601 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG 
     6661 GCGTATCACG AGGCCCTTTC GTC
//