LOCUS       pFS462.xdna    7503 bp      DNA     circular   2/16/2017
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=pFS108
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    5489..6349
                     /label=AmpR
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4739..4899
                     /label=LacZ
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4717..4736
                     /label=T7
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    782..801
                     /label=T3
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4197..4214
                     /label=M13F(-21)
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4517..4540
                     /label=M13F(-47 -41)
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    7289..7381
                     /label=Lac I
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    759..770
                     /label=Lac Z frag
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6729..6751
                     /label=MO205 F1 seq primer
                     /SerialCloner_Color=&h00FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1661..1681
                     /label=MO206 F seq primer
                     /SerialCloner_Color=&h00FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    860..2706
                     /label=His genomic locus
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1000..2253
                     /label=His7 CDS
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2254..2413
                     /label=His7 3' UTR
                     /SerialCloner_Color=&h804040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    928..999
                     /label=His7 5' UTR
                     /SerialCloner_Color=&h804040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2707..3405
                     /label=gal1 promoter with Zif268 bs insertion
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2933..3002
                     /label=Zif268 bs
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3406..4146
                     /label=GFP-SV40 NLS
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4147..4196
                     /label=His7 genomic locus
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4147..4446
                     /label=leu1 post-TAA C-term 300nt
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4447..4532
                     /label=His genomic locus
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 CCAGTGGCTG CTGCCAGGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 
       61 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGTTCG TGCACACAGC CCAGCTTGGA 
      121 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 
      181 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 
      241 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 
      301 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 
      361 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT 
      421 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 
      481 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 
      541 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 
      601 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT 
      661 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT 
      721 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTCGA 
      781 AATTAACCCT CACTAAAGGG AACAAAAGCT GGAGCTCCAC CGCGGTGGCG GCCGCTCTAG 
      841 AACTAGTGGA TCCCCCGGGG ATCTCTTTTG AGTCAGAAAA TCTGACGCAT TGTAACCTGG 
      901 TTACCTAAAG GTGATTTATG ATTGTTACTT GGGGCGATGT GAATCTTTCG TTTGCCAAAC 
      961 AGAAAAGTAG TTAGAGTTTT AGGGGTATTT GACTTACAAA TGGCTCTTTT ACCATTTTTT 
     1021 GATCTTACGA ACTTTGAGAG TGATGCTTCT GAAGAGTTAG GATGGCTTAA GTACGTCGGC 
     1081 AGAGTGCAAA CAAGAGTGTT TCCTCAGCAT TTCAAAGATA ATCTCGAAAA AGTTCGCAAA 
     1141 ATTTCGGAAA CGATTGATGT GATCGTTGAC ACGACTGCTG AACTTGGACC AGAGGCCTGC 
     1201 GTCAACCTTT TGAACGCTGG TGCACTCGCC ATACTGGTTA ATGAAGAAAT GCTAAACGAG 
     1261 TTGGCAGACA TCTCTCCGAA TCGTTTGGTT TTGAAGACAG ACACTACTGA TATTGGAAAA 
     1321 ATTGAAAAAC TTTCACAAGT TGCTGGTTCT ATTCAATGGA TCGGCAGTGC TGAGAATTAT 
     1381 CCCCCTGACT TTTTTGAAAG AGCCAGCAAG ATCATTCACA AAGCTGTAAT GCCTGAGGGT 
     1441 GGAGGCCGAA CTTTATATTT GGAGTTCCCC GAACAACCAT CTATGGAGGT ACTGAAATCT 
     1501 TTTTCTGTTC ACTCTGTCGT TCCCGTTCTG TCAAGTTCTT TCTTGACAGT GAAGCCTGCC 
     1561 GAAGAGCCCA AGAAGTTATC ATTAGCTGAT CTCATTTTGA TATCTGCCAA TACTGATCGT 
     1621 GAGGATGGAC TATTTTCTAC TTTAGTCGTC AATGAGCTCG GTATTGCACT TGGTCTCGTG 
     1681 TATTCTAGCA AGGAAAGCGT TGCTGAGTCT TTGAAGACTG GCACTGGAGT TTATCAATCC 
     1741 CGCAAACGTG GCTTGTGGTA CAAGGGCGCA TCTTCTGGTG CTGTTCAACA CTTAATTCAT 
     1801 ATTGACGTCG ATTGTGATGA AGACTGCTTG CGCTTTGTCG TCTATCAAAC CGGTAAAGGT 
     1861 TTTTGTCATC TTGACACCTT GCATTGCTTT GGACAAGCTT CAGGACTTTG TCAGTTAGAA 
     1921 AAGACTTTAA TCGATAGGAA GAATAATGCT CCTGAGGGAT CTTATACTGC TCGTTTGTTC 
     1981 TCCGACCCAA AGTTATTGCG TGCAAAAATT ATGGAGGAAG CTGAAGAGTT GTGTGATGCT 
     2041 ACCACCAAAG AGAATGTTAT TTGGGAAATG GCTGATTTGA TGTACTTTGC CATAACCCGC 
     2101 TGCGTCGGTT CGGGTGTTAG CCTTAATGAC ATTAGTCGTC ATCTTGATTT GAAACATCGT 
     2161 AAAGTTACAA GAAGAAAGGG AGATGCTAAA GTTGCATGGC AAGAGAAACT TAAAGACAAA 
     2221 GGAGGAGTGG CAAACACGTC TTATACTGCC TGAATTTTTT AAATTTCTAC TCTTCATTCG 
     2281 CAGATGATAG CAGTCGGTCT AAGGTGTAGT CTACTAAAAC CTTGCTAATG CGGAAATTTT 
     2341 TACTTTGTGT AATTATTTAA TAATGAAAAA AAATTCATAT TTGCTGTTTG TACTAATATT 
     2401 ATTTGTTTTA TATTGTTTAA TTTGAGCGCT GGATAGATAA TAACTCAGAC TTCATTAGCC 
     2461 ACTGGCTGGT ATTTGTTGCT CGACTTATTC CTACCAAATA TCGACCTTGT CGCTGTGTAT 
     2521 ACCTTCTGTA ATACACTAGA ATTAGTTGAA CTCTCTGTTT CAACATCATC TGCTTCACTG 
     2581 AATATAAGGC TCTTAGGCTT TTTCCATCTC CTGAGAAGTA AGCTACTTGC AAGAACACTA 
     2641 AGAGAAGAAA ACATCATAGC AGCACTAGCC CACATAGGAT TTAAGTAGAT TCCCCATGGA 
     2701 AGGAAGTTAT ATTGAATTTT CAAAAATTCT TACTTTTTTT TTGGATGGAC GCAAAGAAGT 
     2761 TTAATAATCA TATTACATGG CATTACCACC ATATACATAT CCATATACAT ATCCATATCT 
     2821 AATCTTACTT ATATGTTGTG GAAATGTAAA GAGCCCCATT ATCTTAGCCT AAAAAAACCT 
     2881 TCTCTTTGGA ACTTTCAGTA ATACGCTTAA CTGCTCATTG CTATATTGAA GTGCGGCCGC 
     2941 GTGGGCGTGC GTGGGCGGGC GTGGGCGTGC GTGGGCGGGC GTGGGCGTGC GTGGGCGTCT 
     3001 AGACCGTGCG TCCTCGTCTT CACCGGTCGC GTTCCTGAAA CGCAGATGTG CCTCGCGCCG 
     3061 CACTGCTCCG AACAATAAAG ATTCTACAAT ACTAGCTTTT ATGGTTATGA AGAGGAAAAA 
     3121 TTGGCAGTAA CCTGGCCCCA CAAACCTTCA AATTAACGAA TCAAATTAAC AACCATAGGA 
     3181 TGATAATGCG ATTAGTTTTT TAGCCTTATT TCTGGGGTAA TTAATCAGCG AAGCGATGAT 
     3241 TTTTGATCTA TTAACAGATA TATAAATGGA AAAGCTGCAT AACCACTTTA ACTAATACTT 
     3301 TCAACATTTT CAGTTTGTAT TACTTCTTAT TCAAATGTCA TAAAAGTATC AACAAAAAAT 
     3361 TGTTAATATA CCTCTATACT TTAACGTCAA GGAGAAAAAA CTATAATGAG TAAAGGAGAA 
     3421 GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC 
     3481 AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA 
     3541 TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGCCAA CACTTGTCAC TACTCTCACT 
     3601 TATGGTGTTC AATGCTTTTC AAGATACCCA GATCACATGA AACAGCATGA CTTTTTCAAG 
     3661 AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT TTTTCAAAGA TGACGGGAAC 
     3721 TACAAGACAC GTGCTGAAGT CAAGTTTGAA GGTGATACCC TTGTTAATAG AATCGAGTTA 
     3781 AAAGGTATTG ATTTTAAAGA AGATGGAAAC ATTCTTGGAC ACAAATTGGA ATACAACTAT 
     3841 AACTCACACA ATGTATACAT CATGGCAGAC AAACAAAAGA ATGGAATCAA AGTTAACTTC 
     3901 AAAATTAGAC ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA TCAACAAAAT 
     3961 ACTCCAATTG GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC CACACAATCT 
     4021 GCCCTTTCGA AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA GTTTGTAACA 
     4081 GCTGCTGGGA TTACACATGG CATGGATGAA CTATACAAAG GGCCTAAGAA GAAGCGTAAG 
     4141 GTTTAATAGG GTTTAATGTA GAATAAATTC ATATGATTTG ATTTGACCTA ATTTTTATTG 
     4201 ATTAATTGTG TCGCATATTT ATTATCGTTA AAAATGCAAT TGACAAAAGC GTAAATTTTA 
     4261 AGGCTCTAAT GTTTTTTCTT TTGGTATGTT ATAGTCAAGC AAAGATGTAT TAAGTTGCAA 
     4321 AAGCATGGAA TTGATTTACG ACATCCGTTC AACTTTATGA CCGAGTTGTC GCTGTGTACT 
     4381 TACTCTTTTT TTACTATGAA AAGTTTCAAA CATAATATCA TTAATTATTT CTTTTTAAAC 
     4441 GAAGTAAAGC CCATTGCAAT AGGAATCATG ACAAAGTTAT ATATGCAAGC CCACACAAGG 
     4501 TTCATTTTAA TTCTTTTAAC GATAACTCTA GAGTCGACCT GCAGGCATGC AAGCTTGGCA 
     4561 CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC 
     4621 CTTGCAGCAC ATCCCCCTTT CGCCAGGGGC TGCAGGAATT CGATATCAAG CTTATCGATA 
     4681 CCGTCGACCT CGAGGGGGGG CCCGGTACCC AATTCGCCCT ATAGTGAGTC GTATTACAAT 
     4741 TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT 
     4801 CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT 
     4861 CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGA AATTGTAAGC GTTAATATTT 
     4921 TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA TAGGCCGAAA 
     4981 TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT GTTGTTCCAG 
     5041 TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG 
     5101 TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT TTGGGGTCGA 
     5161 GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG 
     5221 GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG 
     5281 CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC 
     5341 CGCTACAGGG CGCGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 
     5401 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 
     5461 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 
     5521 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 
     5581 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 
     5641 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 
     5701 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 
     5761 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 
     5821 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 
     5881 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 
     5941 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 
     6001 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 
     6061 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 
     6121 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 
     6181 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 
     6241 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 
     6301 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 
     6361 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 
     6421 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 
     6481 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 
     6541 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 
     6601 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 
     6661 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 
     6721 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 
     6781 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 
     6841 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 
     6901 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 
     6961 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 
     7021 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 
     7081 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 
     7141 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA 
     7201 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA 
     7261 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC 
     7321 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG 
     7381 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA 
     7441 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA 
     7501 GCT
//