LOCUS       pPSH23_pGAl1_DIG1_604_( 8592 bp ds-DNA     circular     04-JAN-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(2162..2178)
                     /label="Gal4p-Binding Site"
     misc_feature    2621..3979
                     /label="DIG1"
     primer_bind     complement(5165..5182)
                     /label="M13-fwd"
     misc_feature    2162..2178
                     /label="Gal4p-Binding Site"
     misc_feature    7475..8157
                     /label="Col E1 Origin"
     rep_origin      5339..5645
                     /label="F1 ori"
     misc_feature    1947..2614
                     /label="pGal1"
     misc_feature    complement(2199..2215)
                     /label="Gal4p-Binding Site"
     misc_feature    38..433
                     /label="TRP1 5' UTR"
     misc_feature    2181..2197
                     /label="Gal4p-Binding Site"
     misc_binding    8497..8519
                     /label="LacO"
     misc_feature    2199..2215
                     /label="Gal4p-Binding Site"
     primer_bind     8563..8582
                     /label="T3"
     misc_feature    4048..4742
                     /label="Adh1T"
     misc_feature    436..1931
                     /label="TRP Promoter/ORF/Terminator"
     CDS             6718..7377
                     /label="AmpR"
     primer_bind     8525..8545
                     /label="M13-rev"
     misc_feature    5139..5166
                     /label="T7"
     misc_feature    6718..7376
                     /label="AmpR"
     misc_feature    8525..8545
                     /label="M13-Rev"
     rep_origin      7475..8157
                     /label="ColE1 origin"
     CDS             5253..5321
                     /label="LacZ alpha"
     misc_feature    7613..7629
                     /label="Gal4p-Binding Site"
     misc_feature    complement(2181..2197)
                     /label="Gal4p-Binding Site"
     primer_bind     complement(5139..5166)
                     /label="T7"
     misc_feature    5167..5182
                     /label="M13-Fwd"
     misc_feature    complement(7613..7629)
                     /label="Gal4p-Binding Site"
     misc_feature    4755..5095
                     /label="TRP1 3' UTR"
     misc_feature    929..1844
                     /label="TRP1 ORF"
     misc_feature    5253..5321
                     /label="LacZ α"
     misc_feature    5327..5767
                     /label="F1 ori"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccATA TTTCTACCAA TCTCTCAACA
       61 CTGAGTAATG GTAGTTATAA GAAAGAGACC GAGTTAGGGA CAGTTAGAGG CGGTGGAGAT
      121 ATTCCTTATG GCATGTCTGG CGATGATAAA ACTTTTCAAA CGGCAGCCCC GATCTAAAAG
      181 AGCTGACAGG GAAATGGTCA GAAAAAGAAA CGTGCACCCG CCCGTCTGGA CGCGCCGCTC
      241 ACCCGCACGG CAGAGACCAA TCAGTAAAAA TCAACGGTTA ACGACATTAC TATATATATA
      301 ATATAGGAAG CATTTAATAG AACAGCATCG TAATATATGT GTACTTTGCA GTTATGACGC
      361 CAGATGGCAG TAGTGGAAGA TATTCTTTAT TGAAAAATAG CTTGTCACCT TACGTACAAT
      421 CTTGATCCGG AGCCCGTCGC TTTGAGAGAA ATCAGAAGAT TCCAAAAGTC TACTGAACTT
      481 TTGATCAGAA AGTTGCCTTT CCAAAGACTA GTCAGAGAAA TCGCCCAAGA TTTCAAGACC
      541 GATCTAAGAT TCCAATCCTC TGCCATCGGT GCTCTACAGG AATCCGTTGA AGCTTACTTG
      601 GTCTCTTTGT TCGAAGACAC CAACTTGGCC GCTATCCACG CTAAGCGTGT TACCATCCAA
      661 AAGAAGGATA TCAAGTTGGC TAGAAGATTG AGAGGTGAAA GATCCTAAGC ACACAGGGAT
      721 TGTTGTACAA TTACTTTTTA TGATTTTAGA TACACACAAA TCTTTTCTAC GATTACCTTT
      781 CATTTATTTT CGTTTCATGT TTTATTAATC ATTCTTTTTG TATATTTCAC ATCGGGTGGG
      841 GTTTTTATAT TAAATTTTAA TAACATAATC TTTTAAAGTT TCTACTTTCT ATCCTGTATA
      901 TTATTTCCAT ATCTATATCT ATATTTTCAA GCTTGTTCAC ATCCTTATCG GAATTGTTCC
      961 AGCGTTTTTG TACAGAAAAT GATGTTATGG TAACGAGCTA TATCAGACCT ACTAGTCTTA
     1021 TAGAGCTGTA CAGAGTCTTG GAAACATAAC CAACACACCA ACAAGATGTC ATTTGATTCG
     1081 TTACTCGACA AGAATGATAA GCTGGTAAAA GTTTGCGGGA TTCAAACCGT CGAGGCTGCC
     1141 GAAACTGCGC TTCAAGCAGG CGCTGATTTG ATAGGGATCA TATGTGTCCC CAACAGGAAG
     1201 CGGACTATCG AGAGCGCTGT GGCTCGTGAA ATATCCAAAT TGATTCACAA ATCAGGTACT
     1261 ACAAAGCTGG TGGGGGTGTT CAGGAATCAA TCTGTTGAGG ACGTACATCG GCTTTCTGAG
     1321 GAATATGACC TTGACATAAT CCAATTACAT GGTGATGAAT CATGGCCAGA GTACTATAAC
     1381 GTCATTAAGA AACCAATAAT CAAAAGAGTC ATATTCCCTA GAGATGTCGA TGTTGTAACA
     1441 CAAGTGTGTC AAAGAAAACC CTTGGTATGT CTACCATTGT TCGACTCCGA GGCAGGCGGT
     1501 ACAGGTGAAA AGCTGGACTG GTCCAGTATT TCCTCCTGGG CAAGCGAACA GAATAACGTA
     1561 TTTTATATAC TAGCGGGTGG ACTAACTGCA GAAAACGTCA TGGAGGCGGT GGGGTTGCCT
     1621 GGTGTAATTG GTGTTGACGT CTCAGGTGGT GTAGAGACTG ACGGCGTCAA AGACAATGAC
     1681 AAGATAATTA AATATGTACA AAATGCAAAG AAACAATGAA TTACGAAGAG AAGTCTCAAT
     1741 GAGGTTTTAC AAACAATCCT ATAATACATA TATGCCACTT AGATATACTT TGAATACTGA
     1801 ATATACTGAC TCATTATATT CTTTAATTGT AGTTTCTCAA AAATATTCAT ACAGGTTAGC
     1861 TATGAATTCA TGCTATAATT TCCATTTTGT TCATGTAAGA TACACATAGG GATAAAATGT
     1921 GATAACTAAT CAGCGGTACC GGGCCCTTAT ATTGAATTTT CAAAAATTCT TACTTTTTTT
     1981 TTGGATGGAC GCAAAGAAGT TTAATAATCA TATTACATGG CATTACCACC ATATACATAT
     2041 CCATATCTAA TCTTACTTAT ATGTTGTGGA AATGTAAAGA GCCCCATTAT CTTAGCCTAA
     2101 AAAAACCTTC TCTTTGGAAC TTTCAGTAAT ACGCTTAACT GCTCATTGCT ATATTGAAGT
     2161 ACGGATTAGA AGCCGCCGAG CGGGCGACAG CCCTCCGACG GAAGACTCTC CTCCGTGCGT
     2221 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
     2281 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
     2341 CTGGCCCCAC AAACCTTCAA ATTAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
     2401 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
     2461 TAACAGATAT ATAAATGGAA AAGCTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
     2521 AGTTTGTATT ACTTCTTATT CAAATGTCAT AAAAGTATCA ACAAAAAATT GTTAATATAC
     2581 CTCTATACTT TAACGTCAAG GAGAAAAAAC TATACTCGAG ATGGCCGTAT CAGCCCGTTT
     2641 GAGAACGACT GCCGAGGATA CATCCATTGC TAAATCAACA CAAGATCCAA TTGGTGATAC
     2701 AGAAATCAGT GTAGCAAATG CTAAGGGCAG CAGCGATAGC AACATTAAGA ATTCGCCAGG
     2761 CGGAAACAGC GTTGGTCAGG AGTCGGAGCT AGAGCATGTC CCTGAGGAGG ATGACTCTGG
     2821 TGACAAGGAA GCAGATCATG AGGATTCTGA GACAGCCACT GCGAAGAAGA GGAAAGCCCA
     2881 ACCATTGAAG AATCCGAAGA AATCATTGAA GAGGGGCAGA GTCCCGGCGC CTTTGAATTT
     2941 ATCGGATAGC AACACTAATA CACACGGTGG TAATATTAAG GACGGCAACT TGGCTTCGTC
     3001 TAACTCTGCA CATTTTCCTC CTGTTGCTAA TCAAAACGTG AAAAGCGCGC CCGCACAAGT
     3061 TACTCAGCAT TCCAAGTTCC AGCCCCGTGT CCAATACTTG GGAAAGGCCA GTTCTAGACA
     3121 ATCTATACAA GTGAATAATA GCAGCAATAG TTATGGGAAA CCACACATGC CCTCGGCGGG
     3181 CATCATGAGC GCCATGAACC CTTACATGCC CATGAATCGC TACATAATGT CACCATATTA
     3241 TAATCCGTAC GGTATCCCTC CACCTCACAT GCTGAACAAG CCCATAATGA CACCCTACGT
     3301 GTCGTATCCA TATCCAATGG GACCGCGGAC CTCCATTCCT TATGCTATGC AAGGTGGCAA
     3361 CGCTAGGCCC TACGAAGAAA ATGAGTATAG TGCTAGCAAT TACAGAAACA AGAGGGTTAA
     3421 CGACTCATAT GATTCGCCTT TGAGTGGCAC CGCTTCCACT GGGAAAACCA GACGATCCGA
     3481 GGAAGGCTCA AGAAATTCTA GCGTAGGATC AAGTGCGAAT GCCGGTCCTA CGCAACAGCG
     3541 CGCGGATCTA CGCCCAGCAG ATATGATACC TGCTGAAGAA TACCACTTTG AACGGGATGC
     3601 ATTACTCTCG GCCAACACGA AAGCCAGAAG CGCAAGCACA AGCACAAGTA CAAGCACAAG
     3661 CACAAACCGG GACAGGAGTT CATGGCATGA GGCAGAACCC AATAAAGACG AAGAAGAGGG
     3721 TACGGACTTG GCCATCGAGG ACGGAGCGGT CCCCACTCCC ACTTTTACTA CGTTCCAGCG
     3781 GACCTCGCAG CCGCAGCAGC AATCACCTAG TCTTCTTCAA GGCGAAATCC GACTCTCATC
     3841 GCATATTTTT GCCTTCGAGT TCCCCCTGAG CTCTAGCAAT GTAGACAAGA AAATGTTTAT
     3901 GAGCATATGT AATAAAGTAT GGAATGAATC AAAGGAGCTG ACAAAAAAAT CATCATCACA
     3961 TCACAGAACC GGAAAATAGG GATCCACTAG TTCTAGAcac ctgcaacatg cgTGAGTAAA
     4021 TAAGCGGCCG CCACCGCGGT GGAGCTCTAA GCAAATAGCT AAATTATATA CGAATTAATA
     4081 TTATGATTAA GTGTTTACGT GAGTGCGATA TTTTTATTAC TATCTTATAC AGTTGTATAT
     4141 ACTCTATAAA ATGAGTTGTC TATTAATTAA CGCGATGAAT GCTTTCTGGG TTTACCTCTC
     4201 CAACAACTCT AGTTTACTTC TCAATACATT CAATTGTATT TGATTTGTCA ATACTTCATC
     4261 ATTAATCAAT TCTATAGTTT TGTTTTTCTC GTTTATTTCC AAATTTAATG CATCAATTTT
     4321 ATTATTCAAT TTGTCGTTGA TTTTGGTTAA TGATTTTATG GTTTGATCTC TGGCATTGAT
     4381 TGTTTGTGTT AGTTTTTCAT TATTGATAat taaaTTATTT AAGTTAGTTA TCAACTCGGT
     4441 GTTTTCAAGT TTCAAGTTTT CAATTTCTTT AGAGTTTATT AGATTTGTCA AAGTTTCTGA
     4501 ATTGCTTGAT TGGTCCTGTA GAAGAGTATT TGTTGTTGTG GATAATTGAT TCAATTTTTG
     4561 AGACAATTGC TGGAAGGCGT TGAAATATCT AGCATCAATC TCATGGTTTT TTTCCCGAGA
     4621 GTCTCGTAGA TTCAATTGTT TTAATATATC TTGGGACCAC TCTTGATTTG AACTCATGGA
     4681 AattaaaCTG GGTGTTGTGT TGTGGTGTAA TGATTGTACC CCCTTTGCTT ATAATTGTGT
     4741 GGTTCCGATG CTGACTTaCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC
     4801 CCGGTTATTG CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT
     4861 CCGAAATACT TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC
     4921 AATGATTACG GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCg AGAATACCAA
     4981 GAGTTCCTCG GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA
     5041 CTCAGTGCAG CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAggccgg
     5101 ccatttaaat gtttaaacgg ccacgctggc cCAATTCGCC CTATAGTGAG TCGTATTACA
     5161 ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA
     5221 ATCGCCTTGC AGCACATCCC CCtTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG
     5281 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GACGCGCCCT GTAGCGGCGC
     5341 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT
     5401 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG
     5461 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA
     5521 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT
     5581 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG
     5641 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC
     5701 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT
     5761 ATTAACGcTT ACAATTTCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA
     5821 CACCGCatAG GGTAATAact gatataatta aattgaagct ctaatttgtg agtttagtat
     5881 taggatctct acccttggcg aaaagtcctc tgccaacaat gatgatatct gatccaccac
     5941 ttacaacttc gtcgacggtt ctgtactgct gacccaatat gcgtatatat accaatctaa
     6001 gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc
     6061 aaggaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa aggtggtATG
     6121 GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC
     6181 AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC
     6241 TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC
     6301 GAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT
     6361 TTCTTAgacg tcggcgcgcc GAGGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA
     6421 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA
     6481 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT
     6541 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG
     6601 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG
     6661 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG
     6721 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG
     6781 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA
     6841 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG
     6901 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC
     6961 GCTTTTTTgC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG
     7021 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG
     7081 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC
     7141 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG
     7201 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG
     7261 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGgAG TCAGGCAACT
     7321 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA
     7381 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT
     7441 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG
     7501 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT
     7561 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT
     7621 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG
     7681 CAGATACCAA ATACTGTtCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT
     7741 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC
     7801 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG
     7861 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA
     7921 CTGAGATACC TACAGCGTGA GCtaTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG
     7981 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG
     8041 GGaAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA
     8101 TTTTTGTGAT GCTCGTCAGG GGGGCgGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT
     8161 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT
     8221 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA
     8281 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG
     8341 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG
     8401 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAgC TCACTCATTA GGCACCCCAG
     8461 GCTTTACACT TTATGCTTCC GGCTCCTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT
     8521 CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCG GAATTAACCC TCACTAAAGG
     8581 GAACAAAAGC TG
//