LOCUS       pPSH25_pGAL1_MSG5_604_( 8703 bp ds-DNA     circular     04-JAN-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    8636..8656
                     /label="M13-Rev"
     misc_feature    2621..4090
                     /label="MSG5"
     misc_feature    5250..5277
                     /label="T7"
     misc_feature    5364..5432
                     /label="LacZ α"
     misc_feature    2181..2197
                     /label="Gal4p-Binding Site"
     misc_feature    7586..8268
                     /label="Col E1 Origin"
     misc_feature    4159..4853
                     /label="Adh1T"
     misc_feature    2162..2178
                     /label="Gal4p-Binding Site"
     misc_binding    8608..8630
                     /label="LacO"
     misc_feature    436..1931
                     /label="TRP Promoter/ORF/Terminator"
     rep_origin      7586..8268
                     /label="ColE1 origin"
     primer_bind     complement(5250..5277)
                     /label="T7"
     misc_feature    5278..5293
                     /label="M13-Fwd"
     misc_feature    1947..2614
                     /label="pGal1"
     rep_origin      5450..5756
                     /label="F1 ori"
     CDS             6829..7488
                     /label="AmpR"
     misc_feature    complement(2199..2215)
                     /label="Gal4p-Binding Site"
     CDS             5364..5432
                     /label="LacZ alpha"
     primer_bind     8674..8693
                     /label="T3"
     misc_feature    2199..2215
                     /label="Gal4p-Binding Site"
     primer_bind     complement(5276..5293)
                     /label="M13-fwd"
     misc_feature    complement(2162..2178)
                     /label="Gal4p-Binding Site"
     misc_feature    complement(7724..7740)
                     /label="Gal4p-Binding Site"
     misc_feature    929..1844
                     /label="TRP1 ORF"
     primer_bind     8636..8656
                     /label="M13-rev"
     misc_feature    4866..5206
                     /label="TRP1 3' UTR"
     misc_feature    complement(2181..2197)
                     /label="Gal4p-Binding Site"
     misc_feature    38..433
                     /label="TRP1 5' UTR"
     misc_feature    5438..5878
                     /label="F1 ori"
     misc_feature    7724..7740
                     /label="Gal4p-Binding Site"
     misc_feature    6829..7487
                     /label="AmpR"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccATA TTTCTACCAA TCTCTCAACA
       61 CTGAGTAATG GTAGTTATAA GAAAGAGACC GAGTTAGGGA CAGTTAGAGG CGGTGGAGAT
      121 ATTCCTTATG GCATGTCTGG CGATGATAAA ACTTTTCAAA CGGCAGCCCC GATCTAAAAG
      181 AGCTGACAGG GAAATGGTCA GAAAAAGAAA CGTGCACCCG CCCGTCTGGA CGCGCCGCTC
      241 ACCCGCACGG CAGAGACCAA TCAGTAAAAA TCAACGGTTA ACGACATTAC TATATATATA
      301 ATATAGGAAG CATTTAATAG AACAGCATCG TAATATATGT GTACTTTGCA GTTATGACGC
      361 CAGATGGCAG TAGTGGAAGA TATTCTTTAT TGAAAAATAG CTTGTCACCT TACGTACAAT
      421 CTTGATCCGG AGCCCGTCGC TTTGAGAGAA ATCAGAAGAT TCCAAAAGTC TACTGAACTT
      481 TTGATCAGAA AGTTGCCTTT CCAAAGACTA GTCAGAGAAA TCGCCCAAGA TTTCAAGACC
      541 GATCTAAGAT TCCAATCCTC TGCCATCGGT GCTCTACAGG AATCCGTTGA AGCTTACTTG
      601 GTCTCTTTGT TCGAAGACAC CAACTTGGCC GCTATCCACG CTAAGCGTGT TACCATCCAA
      661 AAGAAGGATA TCAAGTTGGC TAGAAGATTG AGAGGTGAAA GATCCTAAGC ACACAGGGAT
      721 TGTTGTACAA TTACTTTTTA TGATTTTAGA TACACACAAA TCTTTTCTAC GATTACCTTT
      781 CATTTATTTT CGTTTCATGT TTTATTAATC ATTCTTTTTG TATATTTCAC ATCGGGTGGG
      841 GTTTTTATAT TAAATTTTAA TAACATAATC TTTTAAAGTT TCTACTTTCT ATCCTGTATA
      901 TTATTTCCAT ATCTATATCT ATATTTTCAA GCTTGTTCAC ATCCTTATCG GAATTGTTCC
      961 AGCGTTTTTG TACAGAAAAT GATGTTATGG TAACGAGCTA TATCAGACCT ACTAGTCTTA
     1021 TAGAGCTGTA CAGAGTCTTG GAAACATAAC CAACACACCA ACAAGATGTC ATTTGATTCG
     1081 TTACTCGACA AGAATGATAA GCTGGTAAAA GTTTGCGGGA TTCAAACCGT CGAGGCTGCC
     1141 GAAACTGCGC TTCAAGCAGG CGCTGATTTG ATAGGGATCA TATGTGTCCC CAACAGGAAG
     1201 CGGACTATCG AGAGCGCTGT GGCTCGTGAA ATATCCAAAT TGATTCACAA ATCAGGTACT
     1261 ACAAAGCTGG TGGGGGTGTT CAGGAATCAA TCTGTTGAGG ACGTACATCG GCTTTCTGAG
     1321 GAATATGACC TTGACATAAT CCAATTACAT GGTGATGAAT CATGGCCAGA GTACTATAAC
     1381 GTCATTAAGA AACCAATAAT CAAAAGAGTC ATATTCCCTA GAGATGTCGA TGTTGTAACA
     1441 CAAGTGTGTC AAAGAAAACC CTTGGTATGT CTACCATTGT TCGACTCCGA GGCAGGCGGT
     1501 ACAGGTGAAA AGCTGGACTG GTCCAGTATT TCCTCCTGGG CAAGCGAACA GAATAACGTA
     1561 TTTTATATAC TAGCGGGTGG ACTAACTGCA GAAAACGTCA TGGAGGCGGT GGGGTTGCCT
     1621 GGTGTAATTG GTGTTGACGT CTCAGGTGGT GTAGAGACTG ACGGCGTCAA AGACAATGAC
     1681 AAGATAATTA AATATGTACA AAATGCAAAG AAACAATGAA TTACGAAGAG AAGTCTCAAT
     1741 GAGGTTTTAC AAACAATCCT ATAATACATA TATGCCACTT AGATATACTT TGAATACTGA
     1801 ATATACTGAC TCATTATATT CTTTAATTGT AGTTTCTCAA AAATATTCAT ACAGGTTAGC
     1861 TATGAATTCA TGCTATAATT TCCATTTTGT TCATGTAAGA TACACATAGG GATAAAATGT
     1921 GATAACTAAT CAGCGGTACC GGGCCCTTAT ATTGAATTTT CAAAAATTCT TACTTTTTTT
     1981 TTGGATGGAC GCAAAGAAGT TTAATAATCA TATTACATGG CATTACCACC ATATACATAT
     2041 CCATATCTAA TCTTACTTAT ATGTTGTGGA AATGTAAAGA GCCCCATTAT CTTAGCCTAA
     2101 AAAAACCTTC TCTTTGGAAC TTTCAGTAAT ACGCTTAACT GCTCATTGCT ATATTGAAGT
     2161 ACGGATTAGA AGCCGCCGAG CGGGCGACAG CCCTCCGACG GAAGACTCTC CTCCGTGCGT
     2221 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
     2281 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
     2341 CTGGCCCCAC AAACCTTCAA ATTAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
     2401 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
     2461 TAACAGATAT ATAAATGGAA AAGCTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
     2521 AGTTTGTATT ACTTCTTATT CAAATGTCAT AAAAGTATCA ACAAAAAATT GTTAATATAC
     2581 CTCTATACTT TAACGTCAAG GAGAAAAAAC TATACTCGAG ATGCAATTTC ACTCAGATAA
     2641 GCAGCATTTG GACAGTAAAA CCGACATCGA TTTCAAGCCA AACTCACCGC GTTCCTTACA
     2701 AAATAGGAAT ACCAAAAATT TATCTTTAGA TATAGCAGCA CTCCATCCAT TAATGGAATT
     2761 CTCATCGCCA AGCCAAGATG TGCCAGGTTC AGTAAAATTC CCATCGCCGA CACCTTTGAA
     2821 TCTATTTATG AAGCCCAAAC CTATTGTGTT GGAGAAATGT CCACCAAAAG TAAGTCCAAG
     2881 GCCAACGCCA CCATCGCTGT CGATGAGGCG AAGCGAGGCC TCTATATACA CACTACCAAC
     2941 ATCTTTGAAG AACCGAACTG TTTCTCCAAG CGTGTATACA AAATCATCCA CAGTATCGTC
     3001 TATCAGTAAG CTGTCATCAT CATCACCGTT ATCGTCATTT TCAGAAAAAC CTCATCTGAA
     3061 TAGAGTCCAT TCATTATCCG TGAAAACTAA AGACTTGAAG TTGAAGGGAA TTAGAGGACG
     3121 TTCTCAAACG ATCTCAGGGT TAGAGACATC CACGCCAATT TCTAGTACTC GTGAAGGTAC
     3181 TTTAGATAGT ACGGATGTCA ACAGATTTTC TAACCAAAAG AATATGCAAA CAACATTGAT
     3241 TTTCCCGGAG GAGGACTCGG ATCTGAATAT TGATATGGTG CATGCAGAGA TTTATCAACG
     3301 AACGGTTTAT TTAGATGGAC CATTGCTGGT ACTGCCGCCT AATTTGTATC TATATTCAGA
     3361 ACCCAAACTA GAAGATATAT TATCGTTTGA TTTAGTCATT AATGTTGCCA AAGAAATACC
     3421 GAACCTGGAG TTTTTAATAC CGCCGGAAAT GGCACATAAA ATAAAATATT ACCATATTGA
     3481 ATGGACACAC ACATCCAAGA TCGTCAAGGA CTTATCCCGA TTGACACGCA TTATACATAC
     3541 CGCTCATTCG CAAGGCAAGA AAATACTCGT ACACTGTCAG TGTGGAGTAT CAAGATCGGC
     3601 GTCATTGATT GTGGCGTATA TCATGCGATA TTATGGCTTG AGTTTAAATG ATGCATACAA
     3661 TAAGCTGAAA GGTGTTGCTA AGGATATAAG TCCAAACATG GGTCTCATCT TCCAACTTAT
     3721 GGAATGGGGA ACCATGTTGT CCAAGAACTC ACCGGGCGAA GAAGGAGAGA CTGTTCACAT
     3781 GCCTGAGGAA GATGACATCG GAAACAACGA AGTTTCCTCG ACTACGAAGT CCTACTCTTC
     3841 TGCGTCCTTT AGAAGTTTTC CCATGGTAAC GAATCTATCA TCGTCGCCGA ATGACAGTTC
     3901 TGTCAATTCT TCGGAAGTAA CGCCAAGAAC TCCTGCTACG TTGACTGGAG CAAGGACCGC
     3961 ACTGGCCACA GAACGCGGGG AAGATGATGA GCACTGTAAA AGTTTGTCTC AACCCGCAGA
     4021 TTCACTGGAA GCTTCTGTGG ACAACGAATC AATATCTACT GCCCCGGAAC AGATGATGTT
     4081 TCTTCCTTAA GGATCCACTA GTTCTAGAca cctgcaacat gcgTGAGTAA ATAAGCGGCC
     4141 GCCACCGCGG TGGAGCTCTA AGCAAATAGC TAAATTATAT ACGAATTAAT ATTATGATTA
     4201 AGTGTTTACG TGAGTGCGAT ATTTTTATTA CTATCTTATA CAGTTGTATA TACTCTATAA
     4261 AATGAGTTGT CTATTAATTA ACGCGATGAA TGCTTTCTGG GTTTACCTCT CCAACAACTC
     4321 TAGTTTACTT CTCAATACAT TCAATTGTAT TTGATTTGTC AATACTTCAT CATTAATCAA
     4381 TTCTATAGTT TTGTTTTTCT CGTTTATTTC CAAATTTAAT GCATCAATTT TATTATTCAA
     4441 TTTGTCGTTG ATTTTGGTTA ATGATTTTAT GGTTTGATCT CTGGCATTGA TTGTTTGTGT
     4501 TAGTTTTTCA TTATTGATAa ttaaaTTATT TAAGTTAGTT ATCAACTCGG TGTTTTCAAG
     4561 TTTCAAGTTT TCAATTTCTT TAGAGTTTAT TAGATTTGTC AAAGTTTCTG AATTGCTTGA
     4621 TTGGTCCTGT AGAAGAGTAT TTGTTGTTGT GGATAATTGA TTCAATTTTT GAGACAATTG
     4681 CTGGAAGGCG TTGAAATATC TAGCATCAAT CTCATGGTTT TTTTCCCGAG AGTCTCGTAG
     4741 ATTCAATTGT TTTAATATAT CTTGGGACCA CTCTTGATTT GAACTCATGG AAattaaaCT
     4801 GGGTGTTGTG TTGTGGTGTA ATGATTGTAC CCCCTTTGCT TATAATTGTG TGGTTCCGAT
     4861 GCTGACTTaC TGGGTATTAT ATGTGTGCCC AATAGAAAGA GAACAATTGA CCCGGTTATT
     4921 GCAAGGAAAA TTTCAAGTCT TGTAAAAGCA TATAAAAATA GTTCAGGCAC TCCGAAATAC
     4981 TTGGTTGGCG TGTTTCGTAA TCAACCTAAG GAGGATGTTT TGGCTCTGGT CAATGATTAC
     5041 GGCATTGATA TCGTCCAACT GCATGGAGAT GAGTCGTGGC gAGAATACCA AGAGTTCCTC
     5101 GGTTTGCCAG TTATTAAAAG ACTCGTATTT CCAAAAGACT GCAACATACT ACTCAGTGCA
     5161 GCTTCACAGA AACCTCATTC GTTTATTCCC TTGTTTGATT CAGAAggccg gccatttaaa
     5221 tgtttaaacg gccacgctgg ccCAATTCGC CCTATAGTGA GTCGTATTAC AATTCACTGG
     5281 CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG
     5341 CAGCACATCC CCCtTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT
     5401 CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGACGCGCCC TGTAGCGGCG CATTAAGCGC
     5461 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC
     5521 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT
     5581 AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA
     5641 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC
     5701 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT
     5761 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG
     5821 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGcT
     5881 TACAATTTCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCatA
     5941 GGGTAATAac tgatataatt aaattgaagc tctaatttgt gagtttagta ttaggatctc
     6001 tacccttggc gaaaagtcct ctgccaacaa tgatgatatc tgatccacca cttacaactt
     6061 cgtcgacggt tctgtactgc tgacccaata tgcgtatata taccaatcta agtctgtgct
     6121 ccttccttcg ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt caaggaaacc
     6181 gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa aaggtggtAT GGTGCACTCT
     6241 CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC
     6301 TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT
     6361 CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA
     6421 GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAgac
     6481 gtcggcgcgc cGAGGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT
     6541 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA
     6601 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT
     6661 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA
     6721 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC
     6781 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT
     6841 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT
     6901 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT
     6961 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC
     7021 ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTg
     7081 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC
     7141 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA
     7201 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG
     7261 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT
     7321 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT
     7381 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGgA GTCAGGCAAC TATGGATGAA
     7441 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC
     7501 CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC
     7561 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC
     7621 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG
     7681 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG
     7741 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA
     7801 AATACTGTtC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG
     7861 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG
     7921 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA
     7981 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC
     8041 CTACAGCGTG AGCtaTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT
     8101 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGaAACGCC
     8161 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA
     8221 TGCTCGTCAG GGGGGCgGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC
     8281 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG
     8341 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG
     8401 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC
     8461 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC
     8521 AGTGAGCGCA ACGCAATTAA TGTGAGTTAg CTCACTCATT AGGCACCCCA GGCTTTACAC
     8581 TTTATGCTTC CGGCTCCTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA
     8641 AACAGCTATG ACCATGATTA CGCCAAGCTC GGAATTAACC CTCACTAAAG GGAACAAAAG
     8701 CTG
//