LOCUS       pPSH34_pGal1-GPA1_604_( 8655 bp ds-DNA     circular     23-FEB-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    5316..5384
                     /label="LacZ α"
     misc_feature    4040..4042
                     /label="venus"
     primer_bind     complement(5202..5229)
                     /label="T7"
     misc_feature    4818..5158
                     /label="TRP1 3' UTR"
     primer_bind     8588..8608
                     /label="M13-rev"
     primer_bind     complement(5228..5245)
                     /label="M13-fwd"
     misc_feature    929..1844
                     /label="TRP1 ORF"
     misc_feature    7538..8220
                     /label="Col E1 Origin"
     CDS             5316..5384
                     /label="LacZ alpha"
     misc_feature    complement(2162..2178)
                     /label="Gal4p-Binding Site"
     misc_binding    8560..8582
                     /label="LacO"
     misc_feature    complement(2181..2197)
                     /label="Gal4p-Binding Site"
     misc_feature    1947..2614
                     /label="pGal1"
     primer_bind     8626..8645
                     /label="T3"
     misc_feature    2162..2178
                     /label="Gal4p-Binding Site"
     misc_feature    complement(2199..2215)
                     /label="Gal4p-Binding Site"
     misc_feature    5230..5245
                     /label="M13-Fwd"
     misc_feature    complement(7676..7692)
                     /label="Gal4p-Binding Site"
     misc_feature    4111..4805
                     /label="Adh1T"
     rep_origin      7538..8220
                     /label="ColE1 origin"
     CDS             6781..7440
                     /label="AmpR"
     misc_feature    2181..2197
                     /label="Gal4p-Binding Site"
     promoter        8625..8643
                     /label="T3 promoter"
     misc_feature    38..433
                     /label="TRP1 5' UTR"
     misc_feature    2199..2215
                     /label="Gal4p-Binding Site"
     misc_feature    8588..8608
                     /label="M13-Rev"
     misc_feature    5390..5830
                     /label="F1 ori"
     misc_feature    7676..7692
                     /label="Gal4p-Binding Site"
     misc_feature    6781..7439
                     /label="AmpR"
     rep_origin      5402..5708
                     /label="F1 ori"
     misc_feature    436..1931
                     /label="TRP Promoter/ORF/Terminator"
     misc_feature    5202..5229
                     /label="T7"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccATA TTTCTACCAA TCTCTCAACA
       61 CTGAGTAATG GTAGTTATAA GAAAGAGACC GAGTTAGGGA CAGTTAGAGG CGGTGGAGAT
      121 ATTCCTTATG GCATGTCTGG CGATGATAAA ACTTTTCAAA CGGCAGCCCC GATCTAAAAG
      181 AGCTGACAGG GAAATGGTCA GAAAAAGAAA CGTGCACCCG CCCGTCTGGA CGCGCCGCTC
      241 ACCCGCACGG CAGAGACCAA TCAGTAAAAA TCAACGGTTA ACGACATTAC TATATATATA
      301 ATATAGGAAG CATTTAATAG AACAGCATCG TAATATATGT GTACTTTGCA GTTATGACGC
      361 CAGATGGCAG TAGTGGAAGA TATTCTTTAT TGAAAAATAG CTTGTCACCT TACGTACAAT
      421 CTTGATCCGG AGCCCGTCGC TTTGAGAGAA ATCAGAAGAT TCCAAAAGTC TACTGAACTT
      481 TTGATCAGAA AGTTGCCTTT CCAAAGACTA GTCAGAGAAA TCGCCCAAGA TTTCAAGACC
      541 GATCTAAGAT TCCAATCCTC TGCCATCGGT GCTCTACAGG AATCCGTTGA AGCTTACTTG
      601 GTCTCTTTGT TCGAAGACAC CAACTTGGCC GCTATCCACG CTAAGCGTGT TACCATCCAA
      661 AAGAAGGATA TCAAGTTGGC TAGAAGATTG AGAGGTGAAA GATCCTAAGC ACACAGGGAT
      721 TGTTGTACAA TTACTTTTTA TGATTTTAGA TACACACAAA TCTTTTCTAC GATTACCTTT
      781 CATTTATTTT CGTTTCATGT TTTATTAATC ATTCTTTTTG TATATTTCAC ATCGGGTGGG
      841 GTTTTTATAT TAAATTTTAA TAACATAATC TTTTAAAGTT TCTACTTTCT ATCCTGTATA
      901 TTATTTCCAT ATCTATATCT ATATTTTCAA GCTTGTTCAC ATCCTTATCG GAATTGTTCC
      961 AGCGTTTTTG TACAGAAAAT GATGTTATGG TAACGAGCTA TATCAGACCT ACTAGTCTTA
     1021 TAGAGCTGTA CAGAGTCTTG GAAACATAAC CAACACACCA ACAAGATGTC ATTTGATTCG
     1081 TTACTCGACA AGAATGATAA GCTGGTAAAA GTTTGCGGGA TTCAAACCGT CGAGGCTGCC
     1141 GAAACTGCGC TTCAAGCAGG CGCTGATTTG ATAGGGATCA TATGTGTCCC CAACAGGAAG
     1201 CGGACTATCG AGAGCGCTGT GGCTCGTGAA ATATCCAAAT TGATTCACAA ATCAGGTACT
     1261 ACAAAGCTGG TGGGGGTGTT CAGGAATCAA TCTGTTGAGG ACGTACATCG GCTTTCTGAG
     1321 GAATATGACC TTGACATAAT CCAATTACAT GGTGATGAAT CATGGCCAGA GTACTATAAC
     1381 GTCATTAAGA AACCAATAAT CAAAAGAGTC ATATTCCCTA GAGATGTCGA TGTTGTAACA
     1441 CAAGTGTGTC AAAGAAAACC CTTGGTATGT CTACCATTGT TCGACTCCGA GGCAGGCGGT
     1501 ACAGGTGAAA AGCTGGACTG GTCCAGTATT TCCTCCTGGG CAAGCGAACA GAATAACGTA
     1561 TTTTATATAC TAGCGGGTGG ACTAACTGCA GAAAACGTCA TGGAGGCGGT GGGGTTGCCT
     1621 GGTGTAATTG GTGTTGACGT CTCAGGTGGT GTAGAGACTG ACGGCGTCAA AGACAATGAC
     1681 AAGATAATTA AATATGTACA AAATGCAAAG AAACAATGAA TTACGAAGAG AAGTCTCAAT
     1741 GAGGTTTTAC AAACAATCCT ATAATACATA TATGCCACTT AGATATACTT TGAATACTGA
     1801 ATATACTGAC TCATTATATT CTTTAATTGT AGTTTCTCAA AAATATTCAT ACAGGTTAGC
     1861 TATGAATTCA TGCTATAATT TCCATTTTGT TCATGTAAGA TACACATAGG GATAAAATGT
     1921 GATAACTAAT CAGCGGTACC GGGCCCTTAT ATTGAATTTT CAAAAATTCT TACTTTTTTT
     1981 TTGGATGGAC GCAAAGAAGT TTAATAATCA TATTACATGG CATTACCACC ATATACATAT
     2041 CCATATCTAA TCTTACTTAT ATGTTGTGGA AATGTAAAGA GCCCCATTAT CTTAGCCTAA
     2101 AAAAACCTTC TCTTTGGAAC TTTCAGTAAT ACGCTTAACT GCTCATTGCT ATATTGAAGT
     2161 ACGGATTAGA AGCCGCCGAG CGGGCGACAG CCCTCCGACG GAAGACTCTC CTCCGTGCGT
     2221 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
     2281 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
     2341 CTGGCCCCAC AAACCTTCAA ATTAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
     2401 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
     2461 TAACAGATAT ATAAATGGAA AAGCTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
     2521 AGTTTGTATT ACTTCTTATT CAAATGTCAT AAAAGTATCA ACAAAAAATT GTTAATATAC
     2581 CTCTATACTT TAACGTCAAG GAGAAAAAAC TATACTCGAG ATGGGGTGTA CAGTGAGTAC
     2641 GCAAACAATA GGAGACGAAA GTGATCCTTT TCTACAGAAC AAAAGAGCCA ATGATGTCAT
     2701 CGAGCAATCG TTGCAGCTGG AGAAACAACG TGACAAGAAT GAAATAAAAC TGTTACTATT
     2761 AGGTGCCGGT GAGTCAGGTA AATCAACGGT TTTAAAACAA TTAAAATTAT TACATCAAGG
     2821 CGGTTTCTCC CATCAAGAAA GGTTACAGTA TGCTCAAGTG ATATGGGCAG ATGCCATACA
     2881 ATCAATGAAA ATTTTGATTA TTCAGGCCAG AAAACTAGGT ATTCAACTTG ACTGTGATGA
     2941 TCCGATCAAC AATAAAGATT TGTTTGCATG CAAGAGAATA CTGCTAAAGG CTAAAGCTTT
     3001 AGATTATATC AACGCCAGTG TTGCCGGTGG TTCTGATTTT CTAAATGATT ATGTACTGAA
     3061 GTACTCAGAA AGGTATGAAA CTAGGAGGCG TGTTCAGAGT ACCGGACGAG CAAAAGCTGC
     3121 TTTCGATGAA GACGGAAATA TTTCTAATGT CAAAAGTGAC ACTGACAGAG ATGCTGAAAC
     3181 GGTGACGCAA AATGAGGATG CTGATAGAAA CAACAGTAGT AGAATTAACC TACAGGATAT
     3241 TTGCAAGGAC TTGAACCAAG AAGGCGATGA CCAGATGTTT GTTAGAAAAA CATCAAGGGA
     3301 AATTCAAGGA CAAAATAGAC GAAATCTTAT TCACGAAGAC ATTGCTAAGG CAATAAAGCA
     3361 ACTTTGGAAT AACGACAAAG GTATAAAGCA GTGTTTTGCA CGTTCTAATG AGTTTCAATT
     3421 GGAGGGCTCA GCTGCATACT ACTTTGATAA CATTGAGAAA TTTGCTAGTC CGAATTATGT
     3481 CTGTACGGAT GAAGACATTT TGAAGGGCCG TATAAAGACT ACAGGCATTA CAGAAACCGA
     3541 ATTTAACATC GGCTCGTCCA AATTCAAGGT TCTCGACGCT GGTGGGCAGC GTTCTGAACG
     3601 TAAGAAGTGG ATTCATTGTT TCGAAGGAAT TACAGCAGTT TTATTTGTTT TAGCAATGAG
     3661 TGAATACGAC CAGATGTTGT TTGAGGATGA AAGAGTGAAC AGAATGCATG AATCAATAAT
     3721 GCTATTTGAC ACGTTATTGA ACTCTAAGTG GTTCAAAGAT ACACCGTTTA TTTTGTTTTT
     3781 AAATAAAATT GATTTGTTCG AGGAAAAGGT AAAAAGCATG CCCATAAGAA AGTACTTTCC
     3841 TGATTACCAG GGACGTGTCG GCGATGCAGA AGCGGGTCTA AAATATTTTG AGAAGATATT
     3901 TTTGAGCTTG AATAAGACAA ACAAACCAAT CTACGTGAAA CGAACCTGCG CTACCGATAC
     3961 CCAAACTATG AAGTTCGTAT TGAGTGCAGT CACCGATCTA ATCATCCAGC AAAACCTTAA
     4021 AAAAATTGGT ATTATATGAt aaGGATCCAC TAGTTCTAGA cacctgcaac atgcgTGAGT
     4081 AAATAAGCGG CCGCCACCGC GGTGGAGCTC TAAGCAAATA GCTAAATTAT ATACGAATTA
     4141 ATATTATGAT TAAGTGTTTA CGTGAGTGCG ATATTTTTAT TACTATCTTA TACAGTTGTA
     4201 TATACTCTAT AAAATGAGTT GTCTATTAAT TAACGCGATG AATGCTTTCT GGGTTTACCT
     4261 CTCCAACAAC TCTAGTTTAC TTCTCAATAC ATTCAATTGT ATTTGATTTG TCAATACTTC
     4321 ATCATTAATC AATTCTATAG TTTTGTTTTT CTCGTTTATT TCCAAATTTA ATGCATCAAT
     4381 TTTATTATTC AATTTGTCGT TGATTTTGGT TAATGATTTT ATGGTTTGAT CTCTGGCATT
     4441 GATTGTTTGT GTTAGTTTTT CATTATTGAT AattaaaTTA TTTAAGTTAG TTATCAACTC
     4501 GGTGTTTTCA AGTTTCAAGT TTTCAATTTC TTTAGAGTTT ATTAGATTTG TCAAAGTTTC
     4561 TGAATTGCTT GATTGGTCCT GTAGAAGAGT ATTTGTTGTT GTGGATAATT GATTCAATTT
     4621 TTGAGACAAT TGCTGGAAGG CGTTGAAATA TCTAGCATCA ATCTCATGGT TTTTTTCCCG
     4681 AGAGTCTCGT AGATTCAATT GTTTTAATAT ATCTTGGGAC CACTCTTGAT TTGAACTCAT
     4741 GGAAattaaa CTGGGTGTTG TGTTGTGGTG TAATGATTGT ACCCCCTTTG CTTATAATTG
     4801 TGTGGTTCCG ATGCTGACTT aCTGGGTATT ATATGTGTGC CCAATAGAAA GAGAACAATT
     4861 GACCCGGTTA TTGCAAGGAA AATTTCAAGT CTTGTAAAAG CATATAAAAA TAGTTCAGGC
     4921 ACTCCGAAAT ACTTGGTTGG CGTGTTTCGT AATCAACCTA AGGAGGATGT TTTGGCTCTG
     4981 GTCAATGATT ACGGCATTGA TATCGTCCAA CTGCATGGAG ATGAGTCGTG GCgAGAATAC
     5041 CAAGAGTTCC TCGGTTTGCC AGTTATTAAA AGACTCGTAT TTCCAAAAGA CTGCAACATA
     5101 CTACTCAGTG CAGCTTCACA GAAACCTCAT TCGTTTATTC CCTTGTTTGA TTCAGAAggc
     5161 cggccattta aatgtttaaa cggccacgct ggccCAATTC GCCCTATAGT GAGTCGTATT
     5221 ACAATTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC
     5281 TTAATCGCCT TGCAGCACAT CCCCCtTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA
     5341 CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGACGCGC CCTGTAGCGG
     5401 CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC
     5461 CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC
     5521 CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT
     5581 CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC
     5641 GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC
     5701 TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT
     5761 TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA
     5821 AATATTAACG cTTACAATTT CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT
     5881 TCACACCGCa tAGGGTAATA actgatataa ttaaattgaa gctctaattt gtgagtttag
     5941 tattaggatc tctacccttg gcgaaaagtc ctctgccaac aatgatgata tctgatccac
     6001 cacttacaac ttcgtcgacg gttctgtact gctgacccaa tatgcgtata tataccaatc
     6061 taagtctgtg ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat
     6121 ttcaaggaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aaaaggtggt
     6181 ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC
     6241 GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA
     6301 AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG
     6361 CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT
     6421 GGTTTCTTAg acgtcggcgc gccGAGGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC
     6481 GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA
     6541 TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC
     6601 CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA
     6661 ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA
     6721 CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG
     6781 ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA
     6841 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC
     6901 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC
     6961 ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA
     7021 ACCGCTTTTT TgCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG
     7081 CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA
     7141 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA
     7201 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC
     7261 TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA
     7321 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG gAGTCAGGCA
     7381 ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG
     7441 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA
     7501 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT
     7561 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT
     7621 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG
     7681 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA
     7741 GCGCAGATAC CAAATACTGT tCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC
     7801 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT
     7861 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG
     7921 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC
     7981 GAACTGAGAT ACCTACAGCG TGAGCtaTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG
     8041 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA
     8101 GGGGGaAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT
     8161 CGATTTTTGT GATGCTCGTC AGGGGGGCgG AGCCTATGGA AAAACGCCAG CAACGCGGCC
     8221 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC
     8281 CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC
     8341 CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA
     8401 CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC
     8461 TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AgCTCACTCA TTAGGCACCC
     8521 CAGGCTTTAC ACTTTATGCT TCCGGCTCCT ATGTTGTGTG GAATTGTGAG CGGATAACAA
     8581 TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC TCGGAATTAA CCCTCACTAA
     8641 AGGGAACAAA AGCTG
//