LOCUS       pPSH33_pZ3_STE4_603_(HI 8689 bp ds-DNA     circular     23-FEB-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    221..229
                     /label="pZ3 Binding Site"
     primer_bind     6733..6752
                     /label="T3"
     misc_feature    261..269
                     /label="pZ3 Binding Site"
     misc_feature    251..259
                     /label="pZ3 Binding Site"
     primer_bind     complement(3309..3336)
                     /label="T7"
     misc_feature    340..352
                     /label="New Feature"
     CDS             4888..5547
                     /label="AmpR"
     terminator      2674..2728
                     /label="C Albicans Tadh1"
     primer_bind     6695..6715
                     /label="M13-rev"
     misc_feature    231..239
                     /label="pZ3 Binding Site"
     misc_feature    2730..3264
                     /label="HIS 3UTR"
     misc_feature    694..1965
                     /label="STE4"
     misc_feature    6800..7263
                     /label="HIS3 5UTR"
     misc_feature    339..352
                     /label="New Feature"
     CDS             3423..3491
                     /label="LacZ alpha"
     rep_origin      5645..6327
                     /label="ColE1 origin"
     misc_feature    271..279
                     /label="pZ3 Binding Site"
     rep_origin      complement(3497..3937)
                     /label="F1 ori"
     misc_feature    2946..3264
                     /label="HIS3 3UTR"
     terminator      2401..2667
                     /label="C Albicans Tadh1"
     misc_feature    7264..8677
                     /label="CG HIS3 w promoter and terminator"
     misc_feature    336..338
                     /label="New Feature"
     misc_binding    6667..6689
                     /label="LacO"
     primer_bind     complement(3335..3352)
                     /label="M13-fwd"
     terminator      2034..2394
                     /label="C Albicans Tadh1"
     misc_feature    241..249
                     /label="pZ3 Binding Site"
     misc_feature    1..687
                     /label="pZ3QC"
ORIGIN
        1 TTATATTGAA TTTTCAAAAA TTCTTACTTT TTTTTTGGAT GGACGCAAAG AAGTTTAATA
       61 ATCATATTAC ATGGCATTAC CACCATATAC ATATCCATAT CTAATCTTAC TTATATGTTG
      121 TGGAAATGTA AAGAGCCCCA TTATCTTAGC CTAAAAAAAC CTTCTCTTTG GAACTTTCAG
      181 TAATACGCTT AACTGCTCAT TGCTATATTG AAGTGCGGCC GCGTGGGCGT GCGTGGGCGG
      241 GCGTGGGCGT GCGTGGGCGG GCGTGGGCGT GCGTGGGCGT CTAGACCGTG CGTCCTCGTC
      301 TTCACCGGTC GCGTTCCTGA AACGCAGATG TGCCTAATGC CGCACTGCTC CGAACAATAA
      361 AGATTCTACA ATACTAGCTT TTATGGTTAT GAAGAGGAAA AATTGGCAGT AACCTGGCCC
      421 CACAAACCTT CAAATTAACG AATCAAATTA ACAACCATAG GATGATAATG CGATTAGTTT
      481 TTTAGCCTTA TTTCTGGGGT AATTAATCAG CGAAGCGATG ATTTTTGATC TATTAACAGA
      541 TATATAAATG GAAAAGCTGC ATAACCACTT TAACTAATAC TTTCAACATT TTCAGTTTGT
      601 ATTACTTCTT ATTCAAATGT CATAAAAGTA TCAACAAAAA ATTGTTAATA TACCTCTATA
      661 CTTTAACGTC AAGGAGAAAA AACTATACTC GAGATGGCAG CACATCAGAT GGACTCGATA
      721 ACGTATTCTA ATAATGTCAC CCAACAGTAT ATACAACCAC AAAGTCTACA GGATATCTCT
      781 GCAGTGGAGG ATGAAATTCA AAATAAAATA GAGGCCGCCA GACAAGAGAG TAAACAGCTT
      841 CATGCTCAAA TAAATAAAGC AAAACACAAG ATACAAGATG CAAGCTTATT CCAGATGGCC
      901 AACAAAGTTA CTTCGTTGAC CAAAAATAAG ATCAACTTAA AGCCAAATAT CGTGTTGAAA
      961 GGCCATAATA ATAAAATCTC AGATTTTCGG TGGAGTCGAG ATTCAAAACG TATTTTGAGT
     1021 GCAAGTCAAG ATGGCTTTAT GCTTATATGG GACAGTGCTT CAGGTTTAAA ACAGAACGCT
     1081 ATTCCATTAG ATTCTCAATG GGTTCTTTCC TGCGCTATTT CGCCATCGAG TACTTTGGTA
     1141 GCAAGCGCAG GATTAAACAA TAACTGTACC ATTTATAGAG TTTCGAAAGA AAACAGAGTA
     1201 GCGCAAAACG TTGCGTCAAT TTTCAAAGGA CATACTTGCT ATATTTCTGA CATTGAATTT
     1261 ACAGATAACG CACATATATT GACAGCAAGT GGGGATATGA CATGTGCCTT GTGGGATATA
     1321 CCGAAAGCAA AGAGGGTGAG AGAATATTCT GACCATTTAG GTGATGTTTT GGCATTAGCT
     1381 ATTCCTGAAG AGCCAAACTC AGAAAATTCT TCGAACACAT TCGCTAGCTG TGGATCAGAC
     1441 GGGTATACTT ACATATGGGA TAGCAGATCT CCGTCCGCTG TACAAAGCTT TTACGTTAAC
     1501 GATAGTGATA TTAATGCACT TCGTTTTTTC AAAGACGGGA TGTCGATTGT TGCAGGAAGT
     1561 GACAATGGTG CGATAAATAT GTATGATTTA AGGTCGGACT GTTCTATTGC TACTTTTTCT
     1621 CTTTTTCGAG GTTATGAAGA ACGTACCCCT ACCCCTACTT ATATGGCAGC TAACATGGAG
     1681 TACAATACCG CGCAATCGCC ACAAACTTTA AAATCAACAA GCTCAAGCTA TCTAGACAAC
     1741 CAAGGCGTTG TTTCTTTAGA TTTTAGTGCA TCTGGAAGAT TGATGTACTC ATGCTATACA
     1801 GACATTGGTT GTGTTGTGTG GGATGTATTA AAAGGAGAGA TTGTTGGAAA ATTAGAAGGT
     1861 CATGGTGGCA GAGTCACTGG TGTGCGCTCG AGTCCAGATG GGTTAGCTGT ATGTACAGGT
     1921 TCATGGGACT CAACCATGAA AATATGGTCT CCAGGTTATC AATAGGGATC CACTAGTTCT
     1981 AGAcacctgc aacatgcgTG AGTAAATAAG CGGCCGCCAC CGCGGTGGAG CTCTAAGCAA
     2041 ATAGCTAAAT TATATACGAA TTAATATTAT GATTAAGTGT TTACGTGAGT GCGATATTTT
     2101 TATTACTATC TTATACAGTT GTATATACTC TATAAAATGA GTTGTCTATT AATTAACGCG
     2161 ATGAATGCTT TCTGGGTTTA CCTCTCCAAC AACTCTAGTT TACTTCTCAA TACATTCAAT
     2221 TGTATTTGAT TTGTCAATAC TTCATCATTA ATCAATTCTA TAGTTTTGTT TTTCTCGTTT
     2281 ATTTCCAAAT TTAATGCATC AATTTTATTA TTCAATTTGT CGTTGATTTT GGTTAATGAT
     2341 TTTATGGTTT GATCTCTGGC ATTGATTGTT TGTGTTAGTT TTTCATTATT GATAattaaa
     2401 TTATTTAAGT TAGTTATCAA CTCGGTGTTT TCAAGTTTCA AGTTTTCAAT TTCTTTAGAG
     2461 TTTATTAGAT TTGTCAAAGT TTCTGAATTG CTTGATTGGT CCTGTAGAAG AGTATTTGTT
     2521 GTTGTGGATA ATTGATTCAA TTTTTGAGAC AATTGCTGGA AGGCGTTGAA ATATCTAGCA
     2581 TCAATCTCAT GGTTTTTTTC CCGAGAGTCT CGTAGATTCA ATTGTTTTAA TATATCTTGG
     2641 GACCACTCTT GATTTGAACT CATGGAAatt aaaCTGGGTG TTGTGTTGTG GTGTAATGAT
     2701 TGTACCCCCT TTGCTTATAA TTGTGTGGtG ACACCGATTA TTTAAAGCTG CAGCATACGA
     2761 TATATATACA TGTGTATATA TGTATACCTA TGAATGTCAG TAAGTATGTA TACGAACAGT
     2821 ATGATACTGA AGATGACAAG GTAATGCATC ATTCTATACG TGTCATTCTG AACGAGGCGC
     2881 GCTTTCCTTT TTTCTTTTTG CTTTTTCTTT TTTTTTCTCT TGAACTCcAG AAAAAAAATA
     2941 TAAAAGAGAT GGAGGAACGG GAAAAAGTTA GTTGTGGTGA TAGGTGGCAA GTGGTATTCC
     3001 GTAAGAACAA CAAGAAAAGC ATTTCATATT ATGGCTGAAC TGAGCGAACA AGTGCAAAAT
     3061 TTAAGCATCA ACGACAACAA CGAGAATGGT TATGTTCCTC CTCACTTAAG AGGAAAACCA
     3121 AGAAGTGCCA GAAATAACAG TAGCAACTAC AATAACAACA ACGGCGGCTA CAACGGTGGC
     3181 CGTGGCGGTG GCAGCTTCTT TAGCAACAAC CGTCGTGGTG GTTACGGCAA CGGTGGTTTC
     3241 TTCGGTGGAA ACAACGGTGG CAGCggccgg ccatttaaat gtttaaacgg ccacgctggc
     3301 cCAATTCGCC CTATAGTGAG TCGTATTACA ATTCACTGGC CGTCGTTTTA CAACGTCGTG
     3361 ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCtTTCGCCA
     3421 GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA
     3481 ATGGCGAATG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG
     3541 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC
     3601 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG
     3661 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC
     3721 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
     3781 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC
     3841 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA
     3901 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGcTT ACAATTTCCT GATGCGGTAT
     3961 TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCatAG GGTAATAact gatataatta
     4021 aattgaagct ctaatttgtg agtttagtat taggatctct acccttggcg aaaagtcctc
     4081 tgccaacaat gatgatatct gatccaccac ttacaacttc gtcgacggtt ctgtactgct
     4141 gacccaatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct
     4201 gttcggagat taccgaatca aaaaaatttc aaggaaaccg aaatcaaaaa aaagaataaa
     4261 aaaaaaatga tgaattgaaa aggtggtATG GTGCACTCTC AGTACAATCT GCTCTGATGC
     4321 CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG
     4381 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA
     4441 GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT
     4501 TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAgacg tcggcgcgcc GAGGACGTCA
     4561 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT
     4621 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA
     4681 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT
     4741 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG
     4801 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT
     4861 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG
     4921 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG
     4981 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA
     5041 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG
     5101 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTgC ACAACATGGG GGATCATGTA
     5161 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC
     5221 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT
     5281 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA
     5341 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG
     5401 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA
     5461 GTTATCTACA CGACGGGgAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG
     5521 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT
     5581 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT
     5641 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA
     5701 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA
     5761 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT
     5821 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTtCT TCTAGTGTAG
     5881 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA
     5941 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA
     6001 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG
     6061 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCtaTGAGAA
     6121 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA
     6181 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGaAACGCCT GGTATCTTTA TAGTCCTGTC
     6241 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCgGAGC
     6301 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT
     6361 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT
     6421 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG
     6481 GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA
     6541 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT
     6601 GTGAGTTAgC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCCTATG
     6661 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC
     6721 GCCAAGCTCG GAATTAACCC TCACTAAAGG GAACAAAAGC TGggccggcc atttaaatgt
     6781 ttaaacggcc acgctggccG CTGCACGGTC CTGTTCCCTA GCATGTACGT GAGCGTATTT
     6841 CCTTTTAAAC CACGACGCTT TGTCTTCATT CAACGTTTCC CATTGTTTTT TTCTACTATT
     6901 GCTTTGCTGT GGGAAAAACT TATCGAAAGA TGACGACTTT TTCTTAATTC TCGTTTTAAG
     6961 AGCTTGGTGA GCGCTAGGAG TCACTGCCAG GTATCGTTTG AACACGGCAT TAGTCAGGGA
     7021 AGTCATAACA CAGTCCTTTC CCGCAATTTT CTTTTTCTAT TACTCTTGGC CTCCTCTAGT
     7081 ACACTCTATA TTTTTTTATG CCTCGGTAAT GATTTTCATT TTTTTTTTTC CACCTAGCGG
     7141 ATGACTCTTT TTTTTTCTTA GCGATTGGCA TTATCACATA ATGAATTATA CATTATATAA
     7201 AGTAATGTGA TTTCTTCGAA GAATATACTA AAAAATGAGC AGGCAAGATA AACGAAGGCA
     7261 AAGAGGCTCT ATATGTATAT AAAGCTGCGG ATGCCTTCTG ATTTAATCTA GTTCTAGTTT
     7321 ACTTCAGGTT TACTTAAAGT TGAGATAATT GAAGCAAAGA GAAGCATCGA GCAAAGGATG
     7381 GCGTTTGTTA AGAGGGTTAC GCAGGAGACG AATATACAGC TGGCGCTGGA TCTTGACGGT
     7441 GGGTCTGTTT CTGTACGGGA GAGCATACTG GGCAAGGAAT ATGCTAGTGG TGATGGGCAG
     7501 ACCATCCATG TGCACACTGG AGTTGGGTTT TTGGACCACA TGTTGACTGC GCTGGCGAAG
     7561 CATGGCGGGT GGTCTCTGAT CCTGGAGTGT ATAGGGGACT TGCACATCGA TGACCACCAC
     7621 ACTGTTGAGG ACTGTGGGAT TGCGCTGGGC CAAGCGTTCA AGGAGGCGCT TGGCTCCGTG
     7681 CGTGGTATCA AGAGGTTCGG GCATGGGTTT GCACCACTGG ACGAGGCGCT GAGCCGCGCT
     7741 GTGGTTGACT TCTCCAATAG GCCTTTCGCC GTGGTGGAGC TGGGCCTGAA GCGAGAACGC
     7801 ATAGGCCAGC TATCCACAGA GATGATCCCG CACTTCTTGG AGAGTTTCGC CACTGAGGCG
     7861 CGTATCACCA TGCATGTGGA CTGTCTGCGG GGCACCAACG ACCACCACCG CTCCGAATCA
     7921 GCTTTCAAGG CGCTCGCCAT CGCCATCAGA GAGGCAAGAA CACCTACGGG TCGCGATGAC
     7981 GTTCCATCCA CTAAGGGTGT CCTAGCATAG AACACAGCCC ACAGCTACCA CCATCAACAA
     8041 TATTTATATA GATAACGTAC ACATAGAAAT CACACAAACA GAGTATTTAT TCTTAACTAC
     8101 ATGAACTACC ATCAGACCGT CTGGGCCACT ATATAATGTG CTATTCATAA ACGTGATCAC
     8161 TCTACGTAGC AGGCGACCCC AGGTGAAAAT TTTTCAGCGA GCTGCCAGAT TGTCAGGTGA
     8221 AAAACTGAAA AAAACTTCTG GGCGATGAGC TTGTGGCGGG AAATTAAGTA TATAAAGCAG
     8281 TTAGTTTCTT CTGCTTCTTG TGGTTCTGGG TATTCTTGTA ATATTTGGAA TAATAGAAAA
     8341 CCAAGACTAC TGCCTTTCTT TTCATATTAT AGAGGAAGAA ATACGCACGA ACACGATATA
     8401 GAGGTAAATT TTTTGCAAAA AGATACCAAA CTATAAAAGA AATAGAAGAC TAAACTACAA
     8461 AAAATAAAGA AAGTAATACA TATATCCAAT ATGACTGAGT TAGCAGAGCA AGTTTCCATG
     8521 CCTGAAGGGC AACAAAGACG TGGTGGTTAC GTTCCACCTC ACATCCGTAA CGGTGGTGGC
     8581 AGAAGAGGCG ATTCTGCTCC TCGTGGTGGT TTCAACAACG GTGGCGGCCG TGGTGGTTTC
     8641 TTCAGAAACA ACGGTGACCG TGGTGGATTC GGCTTTGGGT ACCGGGCCC
//