LOCUS       Exported                7134 bp ds-DNA     circular SYN 21-FEB-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    3T-4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2017 from SnapGene 3.3.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     27..49
                     /label=T7 R0085
                     /note="T7 R0085"
     primer_bind     27..46
                     /label=T7
                     /note="T7"
     misc_feature    27..46
                     /label=pT7
                     /note="pT7"
     misc_feature    65..1440
                     /label=xba/Bam csy1
                     /note="xba/Bam csy1"
     misc_feature    65..70
                     /label=XbaI
                     /note="XbaI"
     misc_feature    87..106
                     /label=Lic_v2_For(2)
                     /note="Lic_v2_For(2)"
     RBS             93..98
                     /label=RBS
     misc_feature    111..121
                     /label=Start of Csy1
                     /note="Start of Csy1"
     misc_feature    1017..1022
                     /label=PstI
                     /note="PstI"
     RBS             1383..1388
                     /label=RBS(1)
                     /note="RBS(1)"
     misc_feature    1420..1434
                     /label=Lic_v2_rev(2)
                     /note="Lic_v2_rev(2)"
     misc_feature    1435..1440
                     /label=BamHI
                     /note="BamHI"
     primer_bind     1459..1476
                     /label=M13-fwd
                     /note="M13-fwd"
     misc_feature    1477..1482
                     /label=EcoRI
                     /note="EcoRI"
     misc_feature    1483..2553
                     /label=psp/asi csy2
                     /note="psp/asi csy2"
     misc_feature    1484..1489
                     /label=XhoI
                     /note="XhoI"
     misc_feature    1491..1496
                     /label=XbaI(1)
                     /note="XbaI(1)"
     misc_feature    1513..1532
                     /label=Lic_v2_For(1)
                     /note="Lic_v2_For(1)"
     RBS             1519..1524
                     /label=RBS(2)
                     /note="RBS(2)"
     misc_feature    1690..1695
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    1740..1745
                     /label=EcoRV(1)
                     /note="EcoRV(1)"
     misc_feature    2525..2539
                     /label=Lic_v2_rev(1)
                     /note="Lic_v2_rev(1)"
     misc_feature    2540..2545
                     /label=BamHI(1)
                     /note="BamHI(1)"
     misc_feature    2574..3759
                     /label=sbf/asc his6 csy3
                     /note="sbf/asc his6 csy3"
     misc_feature    2575..2580
                     /label=PstI(1)
                     /note="PstI(1)"
     misc_feature    2583..2588
                     /label=XhoI(1)
                     /note="XhoI(1)"
     misc_feature    2590..2595
                     /label=XbaI(2)
                     /note="XbaI(2)"
     RBS             2618..2623
                     /label=RBS(3)
                     /note="RBS(3)"
     misc_feature    2644..2661
                     /label=6His tag
                     /note="6His tag"
     misc_feature    2662..2688
                     /label=TEV protease site
                     /note="TEV protease site"
     misc_feature    2671..2685
                     /label=Lic_v1_For
                     /note="Lic_v1_For"
     misc_feature    2911..2916
                     /label=PstI(2)
                     /note="PstI(2)"
     misc_feature    3722..3736
                     /label=Lic_v1_rev
                     /note="Lic_v1_rev"
     misc_feature    3738..3743
                     /label=BamHI(2)
                     /note="BamHI(2)"
     misc_feature    3779..4461
                     /label=not/dra csy4
                     /note="not/dra csy4"
     misc_feature    3779..3786
                     /label=NotI
                     /note="NotI"
     misc_feature    3788..3793
                     /label=PstI(3)
                     /note="PstI(3)"
     misc_feature    3796..3801
                     /label=XhoI(2)
                     /note="XhoI(2)"
     misc_feature    3803..3808
                     /label=XbaI(3)
                     /note="XbaI(3)"
     misc_feature    3825..3844
                     /label=Lic_v2_For
                     /note="Lic_v2_For"
     RBS             3831..3836
                     /label=RBS(4)
                     /note="RBS(4)"
     misc_feature    3849..3866
                     /label=Csy4 starting seq
                     /note="Csy4 starting seq"
     RBS             4354..4359
                     /label=RBS(5)
                     /note="RBS(5)"
     RBS             4384..4389
                     /label=RBS(6)
                     /note="RBS(6)"
     misc_feature    4417..4431
                     /label=Lic_v2_rev
                     /note="Lic_v2_rev"
     misc_feature    4432..4437
                     /label=BamHI(3)
                     /note="BamHI(3)"
     misc_feature    4480..4501
                     /label=Cassette 5
                     /note="Cassette 5"
     misc_feature    4508..4513
                     /label=XhoI(3)
                     /note="XhoI(3)"
     misc_feature    4578..4625
                     /label=b0016
                     /note="b0016"
     RBS             4630..4635
                     /label=RBS(7)
                     /note="RBS(7)"
     misc_feature    4648..4653
                     /label=SpeI
                     /note="SpeI"
     misc_feature    4784..5644
                     /label=B-Lactamase
                     /note="B-Lactamase"
     RBS             complement(5662..5667)
                     /label=RBS(8)
                     /note="RBS(8)"
     misc_feature    complement(5754..5863)
                     /label=RNA1-for
                     /note="RNA1-for"
     misc_feature    complement(6302..6311)
                     /label=ColE1 ORI
                     /note="ColE1 ORI"
     RBS             complement(6508..6513)
                     /label=RBS(9)
                     /note="RBS(9)"
     misc_feature    complement(6732..6957)
                     /label=ROP/ROM
                     /note="ROP/ROM"
     misc_feature    6733..6924
                     /label=ROP
                     /note="ROP"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctctaga aataattttg tttaacttta agaaggagat atagatcatg acctctcccc
      121 tcccaacgcc tacgtggcag gagcttcgcc agttcatcga atccttcatc caggagcgcc
      181 tccagggcaa gctggacaaa ctccaacccg acgaagacga caagcgccag acattgctgg
      241 ccacccaccg gcgggaggcc tggctggccg atgccgcccg gcgggttggc cagttgcagt
      301 tggtgaccca cacgctcaag ccgatccatc ccgacgcccg cggcagcaac ctgcacagcc
      361 tgccgcaagc acccggccaa ccgggcctcg ccggttccca tgagctaggt gacaggctgg
      421 tcagcgatgt ggtgggcaat gccgcggcgc tggacgtatt caagtttctc agtctccagt
      481 atcagggtaa aaatcttctg aactggctga cagaagacag tgccgaggca ttacaggcgc
      541 tgtccgataa cgccgaacag gctcgcgaat ggcggcaagc gttcatcggc atcacgaccg
      601 tcaaaggcgc tcccgcgtcc cacagcctgg ccaagcagtt gtactttccc ctgcccggtt
      661 ccggctacca cctgctagca ccgctgtttc ccaccagtct ggtgcatcac gtccacgctc
      721 tgctccgcga agcgcgcttc ggcgacgcgg ccaaggcggc acgcgaagcg cgcagccgcc
      781 aggagtcatg gccccacgga ttcagcgagt accccaacct ggcgatccag aagttcggcg
      841 gtaccaagcc gcagaacatc agtcagttga acaacgagcg ccgtggcgag aactggttgc
      901 tgccatcgct tccgccgaac tggcaaaggc agaatgtaaa cgcgccgatg cggcactctt
      961 cggtcttcga gcatgacttc ggaagaactc ctgaagtatc ccgcctaacc cgtaccctgc
     1021 agcgttttct tgccaagaca gtccataaca acctcgccat ccgccagcgg cgtgcccaat
     1081 tggtggcgca aatctgcgac gaagccctgc aatacgccgc ccgtctgcgc gaactggagc
     1141 ccggctggag cgcaaccccc gggtgccaac tgcatgacgc ggagcagctc tggctcgatc
     1201 cgttgcgcgc acagaccgat gagacgttct tgcagcgccg actacgaggt gactggcctg
     1261 ccgaggtcgg aaatcgcttc gccaactggc tgaaccgggc ggtcagcagc gacagtcaga
     1321 tactgggtag cccggaagcc gcccaatgga gccaggagct gagcaaggaa ctgacgatgt
     1381 tcaaggagat actcgaagat gagcgtgact gataataaga tcccaactcc ataaggatcc
     1441 gggccctgta caagatcctg taaaacgacg gccagtgaat tcactcgagt tctagaaata
     1501 attttgttta actttaagaa ggagatatag atcatgagcg tgactgatcc cgaggcgctg
     1561 ctgttgctgc cacgcctgtc catccagaac gccaacgcca tttccagccc cttgacctgg
     1621 ggtttccctt cgcccggcgc tttcaccgga ttcgtccatg ccttgcagcg tcgagtcgga
     1681 atctccctcg atatcgaact ggacggtgtc ggcatcgtct gtcaccgctt cgaagcgcag
     1741 atatcgcaac cggccggcaa gcgcaccaag gtcttcaacc tgacccgcaa tccgctgaac
     1801 cgcgacggct ccaccgcagc catcgtcgaa gaaggtcgcg cccatctgga ggtcagtctg
     1861 ctgctcggag tgcatggcga tggtctggac gatcaccccg cacaggaaat cgccaggcag
     1921 gtacaggagc aggctggcgc catgcgcctc gccggtggca gcatcctgcc ctggtgcaat
     1981 gagcgcttcc cggctccgaa cgccgaactg ctgatgctgg gtggcagcga cgaacagcgg
     2041 cgcaagaacc agcgccggct tacccgccgc ctgctccccg gcttcgccct ggtgagtcgc
     2101 gaggcgttgt tgcaacaaca tctggaaacc ttgcgcacca cgctccccga agccaccacg
     2161 ctcgatgcac tgctcgatct ttgtcgaatc aacttcgagc ctcctgcaac ctcgtccgaa
     2221 gaagaagcat cgccgcccga tgcagcctgg caagtgcgcg acaagcccgg ctggctggtg
     2281 ccgatcccgg ctggctacaa cgccctgtca cccctgtacc tccccggcga agtgcgaaat
     2341 gcccgtgata gagaaacacc gctgcgcttc gtcgaaaacc tcttcggtct gggcgaatgg
     2401 ctcagccccc atcgcgtcgc cgcgttatcg gacctgcttt ggtatcacca cgccgagcct
     2461 gacaaagggc tctaccgctg gagcacaccc cgtttcgtcg aacacgccat cgcataataa
     2521 taagatccca actccataag gatccgcgat cgcagcggat aacaatttca catcctgcag
     2581 gactcgagtt ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgaaatct
     2641 tctcaccatc accatcacca tgaaaacctg tacttccaat ccaatgcatc caagccaata
     2701 ctgagcaccg cttccgtcct cgccttcgaa cgtaagctcg acccttccga cgcccttatg
     2761 agcgctggcg cctgggcgca acgcgacgcc tcgcaggaat ggccggccgt gaccgtgcgc
     2821 gagaagtccg tacgcggcac catctccaac cgcctcaaga ccaaggaccg tgacccggcc
     2881 aagctggacg cctcgatcca gtcgcccaac ctgcagacgg tggacgtggc caacctgccg
     2941 agcgacgccg acaccctcaa ggtccgcttc accctccgtg tgctcggcgg cgccggaaca
     3001 ccgtctgcct gcaacgacgc ggcctaccgt gacaagctgc tgcaaacggt cgccacctac
     3061 gtgaacgatc agggcttcgc cgagctggct cgtcgttatg cgcacaacct ggccaacgcc
     3121 cgcttcctgt ggcgcaaccg ggtgggcgcg gaagcggtgg aagtccgtat caaccatatc
     3181 cgccagggcg aggtggctcg cgcctggcgc ttcgacgccc tggccatcgg cttgcgcgac
     3241 ttcaaggccg acgccgaact ggacgcgctc gccgaactga tcgccagcgg tctctcaggc
     3301 agtgggcatg tcctgctcga agtggtcgcc ttcgcccgta tcggcgacgg ccaggaagtc
     3361 ttcccctccc aggaactgat cctcgacaaa ggcgacaaga aaggccagaa gagcaagacc
     3421 ctgtacagcg tacgcgatgc cgcggccatc cactcgcaga agatcggcaa tgccctgcgc
     3481 accatcgata cgtggtatcc cgacgaagat ggcctcggcc ccatcgccgt ggagccctac
     3541 ggctccgtca catcccaggg caaagcctat cgccagccca agcagaagct ggacttctat
     3601 acgctgctcg acaactgggt actgcgcgac gaggcgcccg ccgtggagca acagcattat
     3661 gtgatcgcca acctgatccg tggcggcgtg ttcggtgaag ccgaagagaa gtaataataa
     3721 cattggaagt ggataacgga tccgcgatcg cggcgcgccc gccagggttt tcccagtcgc
     3781 ggccgccctg caggactcga gttctagaaa taattttgtt taactttaag aaggagatat
     3841 agatcatgga ccactacctc gacattcgct tgcgaccgga cccggaattt cccccggcgc
     3901 aactcatgag cgtgctcttc ggcaagctcc accaggccct ggtggcacag ggcggggaca
     3961 ggatcggcgt gagcttcccc gacctcgacg aaagccgctc ccggctgggc gagcgcctgc
     4021 gcattcatgc ctcggcggac gaccttcgtg ccctgctcgc ccggccctgg ctggaagggt
     4081 tgcgggacca tctgcaattc ggagaaccgg cagtcgtgcc tcaccccaca ccgtaccgtc
     4141 aggtcagtcg ggttcaggcg aaaagcaatc cggaacgcct gcggcggcgg ctcatgcgcc
     4201 ggcacgatct gagtgaggag gaggctcgga aacgcattcc cgatacggtc gcgagagcct
     4261 tggacctgcc cttcgtcacg ctacgcagcc agagcaccgg acagcacttc cgtctcttca
     4321 tccgccacgg gccgttgcag gtgacggcag aggaaggagg attcacctgt tacgggttga
     4381 gcaaaggagg tttcgttccc tggttctgat aataagatcc caactccata aggatccgcg
     4441 atcgcggcgc gccacctggt gccatcgccc tgatagacgt taattaaagt actggccggc
     4501 cacgcgtctc gagagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
     4561 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
     4621 ttttgctgaa aggaggaact atatccgact agtagcccgc ctaatgagcg ggcttttttt
     4681 taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4741 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4801 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4861 aacgctcgtg aaagtaaaag acgcagagga ccaattgggg gcacgagtgg gatacataga
     4921 actggacttg aatagcggta aaatccttga gagttttcgc cctgaagagc gttttccaat
     4981 gatgagcact ttcaaagttc tgctatgtgg agcagtatta tcccgtgtag atgcggggca
     5041 agagcaactc ggacgacgaa tacactattc gcagaatgac ttggttgaat actccccagt
     5101 gacagaaaag caccttacgg acggaatgac ggtaagagaa ttatgtagtg ccgccataac
     5161 gatgagtgat aacactgcgg cgaacttact tctgacaacc atcggtggac cgaaggaatt
     5221 aaccgctttt ttgcacaata tgggagacca tgtaactcgc cttgaccgtt gggaaccaga
     5281 actgaatgaa gccataccaa acgacgagcg agacaccaca atgcctgcgg caatggcaac
     5341 aacattacgc aaactattaa ctggcgaact acttactctg gcttcacggc aacaattaat
     5401 agactggctt gaagcggata aagttgcagg accactactg cgttcggcac ttcctgctgg
     5461 ctggtttatt gctgataaat ctggggcagg agagcgtggt tcacggggta tcattgccgc
     5521 acttggacca gatggtaagc cttcccgtat cgtagttatc tacacgacgg gtagtcaggc
     5581 aactatggac gaacgaaata gacagattgc tgaaataggg gcttcactga ttaagcattg
     5641 gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacgg accggtagaa
     5701 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5761 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5821 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     5881 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5941 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6001 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6061 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6121 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6181 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6241 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6301 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6361 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6421 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6481 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     6541 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     6601 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     6661 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     6721 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta
     6781 aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag
     6841 ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag
     6901 ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg
     6961 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat
     7021 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag
     7081 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgtt
//
