LOCUS       Exported                2843 bp ds-DNA     circular SYN 21-FEB-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    plasmid_36
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2017 from SnapGene 3.3.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     292..309
                     /label=M13-fwd
                     /note="M13-fwd"
     misc_feature    320..339
                     /label=pT7
                     /note="pT7"
     misc_feature    370..375
                     /label=SacI
                     /note="SacI"
     misc_feature    397..416
                     /label=pT7(1)
                     /note="pT7(1)"
     repeat_region   416..443
                     /note="PaDR"
     repeat_region   476..503
                     /note="PaDR(1)"
     repeat_region   536..563
                     /note="PaDR(2)"
     repeat_region   596..623
                     /note="PaDR(3)"
     repeat_region   656..683
                     /note="PaDR(4)"
     repeat_region   716..743
                     /note="PaDR(5)"
     repeat_region   776..803
                     /note="PaDR(6)"
     repeat_region   836..863
                     /note="PaDR(7)"
     misc_feature    917..922
                     /label=XhoI
                     /note="XhoI"
     misc_feature    929..934
                     /label=EcoRI
                     /note="EcoRI"
     rep_origin      complement(1141..1823)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ColE1 origin"
     misc_feature    2150..2155
                     /label=NcoI
                     /note="NcoI"
     misc_feature    2165..2170
                     /label=BglII
                     /note="BglII"
     misc_feature    2533..2538
                     /label=PstI
                     /note="PstI"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatg agctcgcgac tcctgcatta ggaaattaat acgactcact atagggttca
      421 ctgccgtgta ggcagctaag aaattcacgg cgggcttgat gtccgcgtct acctggttca
      481 ctgccgtgta ggcagctaag aaattcacgg cgggcttgat gtccgcgtct acctggttca
      541 ctgccgtgta ggcagctaag aaattcacgg cgggcttgat gtccgcgtct acctggttca
      601 ctgccgtgta ggcagctaag aaattcacgg cgggcttgat gtccgcgtct acctggttca
      661 ctgccgtgta ggcagctaag aaattcacgg cgggcttgat gtccgcgtct acctggttca
      721 ctgccgtgta ggcagctaag aaattcacgg cgggcttgat gtccgcgtct acctggttca
      781 ctgccgtgta ggcagctaag aaattcacgg cgggcttgat gtccgcgtct acctggttca
      841 ctgccgtgta ggcagctaag aaatagcata accccttggg gcctctaaac gggtcttgag
      901 gggttttttg ggtaccctcg agtctggtga attcctgggc ctcatgggcc ttcctttcac
      961 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaacatggt catagctgtt
     1021 tccttgcgta ttgggcgctc tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1081 gggtaaagcc tggggtgcct aatgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1141 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1201 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1261 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1321 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1381 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1441 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1501 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1561 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1621 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1681 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1741 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1801 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1861 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1921 ttagaaaaat tcatccagca gacgataaaa cgcaatacgc tggctatccg gtgccgcaat
     1981 gccatacagc accagaaaac gatccgccca ttcgccgccc agttcttccg caatatcacg
     2041 ggtggccagc gcaatatcct gataacgatc cgccacgccc agacggccgc aatcaataaa
     2101 gccgctaaaa cggccatttt ccaccataat gttcggcagg cacgcatcac catgggtcac
     2161 caccagatct tcgccatccg gcatgctcgc tttcagacgc gcaaacagct ctgccggtgc
     2221 caggccctga tgttcttcat ccagatcatc ctgatccacc aggcccgctt ccatacgggt
     2281 acgcgcacgt tcaatacgat gtttcgcctg atgatcaaac ggacaggtcg ccgggtccag
     2341 ggtatgcaga cgacgcatgg catccgccat aatgctcact ttttctgccg gcgccagatg
     2401 gctagacagc agatcctgac ccggcacttc gcccagcagc agccaatcac ggcccgcttc
     2461 ggtcaccaca tccagcaccg ccgcacacgg aacaccggtg gtggccagcc agctcagacg
     2521 cgccgcttca tcctgcagct cgttcagcgc accgctcaga tcggttttca caaacagcac
     2581 cggacgaccc tgcgcgctca gacgaaacac cgccgcatca gagcagccaa tggtctgctg
     2641 cgcccaatca tagccaaaca gacgttccac ccacgctgcc gggctacccg catgcaggcc
     2701 atcctgttca atcatactct tcctttttca atattattga agcatttatc agggttattg
     2761 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     2821 cacatttccc cgaaaagtgc cac
//
