LOCUS       Exported                7305 bp ds-DNA     circular SYN 22-FEB-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pET151-Vps4p(101-437. R325D)-(GGGGS)3GGG-Hcp1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Feb 22, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
COMMENT     CPH 2484 made by Han Han / Binita Shakya
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     209..228
                     /note="T7"
     misc_feature    228..252
                     /note="lacO"
     misc_binding    228..250
                     /note="LacO"
     CDS             300..317
                     /note="6His"
     CDS             318..359
                     /note="V5 tag"
     CDS             360..380
                     /note="TEV site"
     CDS             360..379
                     /note="TEV site"
     CDS             381..1391
                     /codon_start=1
                     /note="Vps4p(101-437) R325D"
                     /translation="QEEGEDNGGEDNKKLRGALSSAILSEKPNVKWEDVAGLEGAKEAL
                     KEAVILPVKFPHLFKGNRKPTSGILLYGPPGTGKSYLAKAVATEANSTFFSVSSSDLVS
                     KWMGESEKLVKQLFAMARENKPSIIFIDEVDALTGTRGEGESEASRRIKTELLVQMNGV
                     GNDSQGVLVLGATNIPWQLDSAIRRRFERRIYIPLPDLAARTTMFEINVGDTPCVLTKE
                     DYDTLGAMTEGYSGSDIAVVVKDALMQPIRKIQSATHFKDVSTEDDETRKLTPCSPGDD
                     GAIEMSWTDIEADELKEPDLTIKDFLKAIKSTRPTVNEDDLLKQEQFTRDFGQEGN"
     misc_feature    1446..1934
                     /note="Hcp1"
                     /note="note=""direct strand 
                     translation""translation=""MAVDMFIKIGDVKGESKDKTHAEEIDVLAWSW
                     GMSQSGSMHMGGGGGAGKVNVQDLSFTKYIDKSTPNLMMACSSGKHYPQAKLTIRKAGG
                     ENQVEYLIITLKEVLVSSVSTGGSGGEDRLTENVTLNFAQVQVDYQPQKADGAKDGGPV
                     KYGWNIRQNVQAG"""
     promoter        2423..2451
                     /note="Amp prom"
     CDS             2691..3350
                     /note="AmpR"
     rep_origin      3502..4130
                     /direction=RIGHT
                     /note="ColE1 origin"
     misc_feature    complement(6045..7136)
                     /note="lacI"
ORIGIN
        1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
       61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
      181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg
      241 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat atacatatgc
      301 atcatcacca tcaccatggt aagcctatcc ctaaccctct cctcggtctc gattctacgg
      361 aaaacctgta ttttcaggga caggaagagg gtgaggataa tggtggagaa gacaacaaaa
      421 agctaagagg tgccttatcg agtgctattt tgtctgagaa gcccaatgtt aagtgggaag
      481 acgtggctgg actggaagga gccaaagagg cccttaaaga agccgttatt ttacctgtca
      541 agttcccaca tttgttcaaa ggtaaccgta agcccacttc gggaatctta ttgtacggac
      601 caccaggtac aggtaaatca tatttggcaa aagcagtggc caccgaggcc aattctacct
      661 ttttcagtgt tagttccagt gatttggttt ctaagtggat gggtgaatct gaaaaacttg
      721 tcaaacagtt atttgcgatg gcaagagaaa ataaaccttc tattattttt atagatgaag
      781 tggatgcgct aacaggtacc agaggagaag gagagagcga agcaagtaga agaattaaaa
      841 cagaattatt ggtacaaatg aatggtgttg ggaatgactc ccaaggtgtt ttggtccttg
      901 gcgcaacaaa tatcccatgg caactggaca gtgccatcag gagaagattt gaaagaagaa
      961 tatatatacc attaccagat ttggcggcaa gaaccaccat gttcgagatt aacgtcggcg
     1021 acactccatg cgtattgacc aaggaagatt atgatacttt aggtgcaatg actgaaggtt
     1081 attccgggag tgatattgct gtagtggtga aagatgcgct aatgcaaccg ataagaaaaa
     1141 ttcaaagcgc gacacatttc aaagatgtct ctacggagga cgacgaaaca agaaaactaa
     1201 caccatgctc tccaggtgat gatggcgcca tcgagatgag ttggacggat attgaagctg
     1261 atgaattaaa agaaccagat ctcaccataa aggatttctt aaaggctatc aaatcgacga
     1321 gacctaccgt gaatgaggat gacttgctga agcaagaaca gttcactaga gattttggtc
     1381 aagaaggtaa cggtggtggc gggtctggag gtggaggcag tggcggaggt gggagcggag
     1441 gcgggatggc tgttgatatg ttcatcaaga tcggcgacgt caagggtgag tccaaggaca
     1501 agactcacgc cgaggaaatc gacgtgctgg catggagctg gggcatgtcc cagtccgggt
     1561 cgatgcacat gggcggtggc ggcggcgccg gcaaggtcaa cgtgcaggac ctgtcgttca
     1621 ccaagtacat cgacaagtcc acgcccaacc tgatgatggc ctgctccagc ggcaagcact
     1681 atccgcaggc gaagctgacc atccgcaagg ccggcggcga gaaccaggtc gagtacctga
     1741 tcatcaccct gaaggaagtc ctggtgtcct cggtgagcac cggcggcagc ggtggcgagg
     1801 atcgcctgac cgagaacgtg accctgaact tcgcccaggt ccaggtcgac taccagccgc
     1861 agaaggcgga tggcgcgaag gacggcggtc cggtcaagta cggctggaac atccgccaga
     1921 acgtgcaggc cggataataa aagggcgagc tcagatccgg ctgctaacaa agcccgaaag
     1981 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
     2041 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tcccgcaaga
     2101 ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga
     2161 ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta
     2221 actgtgataa actaccgcat taaagctagc ttatcgatga taagctgtca aacatgagaa
     2281 ttaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     2341 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2401 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2461 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2521 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2581 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2641 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2701 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     2761 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2821 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2881 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2941 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3001 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca
     3061 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3121 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3181 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3241 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3301 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3361 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3421 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3481 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3541 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3601 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3661 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3721 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3781 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3841 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3901 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3961 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4021 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4081 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4141 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4201 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4261 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     4321 cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga
     4381 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     4441 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4501 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4561 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca
     4621 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
     4681 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
     4741 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
     4801 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
     4861 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
     4921 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
     4981 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
     5041 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
     5101 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
     5161 cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc
     5221 gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg
     5281 ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg
     5341 aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac
     5401 cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc
     5461 cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg
     5521 aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta
     5581 cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa
     5641 tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg
     5701 cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac
     5761 cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga
     5821 tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca
     5881 cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc
     5941 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
     6001 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc
     6061 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6121 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga
     6181 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
     6241 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg
     6301 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg
     6361 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg
     6421 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc
     6481 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc
     6541 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat
     6601 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg
     6661 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc
     6721 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc
     6781 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac
     6841 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac
     6901 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc
     6961 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt
     7021 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa
     7081 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg
     7141 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg
     7201 gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
     7261 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatg
//
