LOCUS       Exported                9272 bp ds-DNA     circular SYN 26-MAY-2017
DEFINITION  humanized LbCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3.1-CMV-NLS-hLbCpf1(RR)-NLS-3xHA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Linyi Gao
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 26, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9272
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             923..973
                     /codon_start=1
                     /label=NLS-SV40
                     /note="NLS-SV40"
                     /translation="MAPKKKRKVGIHGVPAA"
     CDS             974..4654
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /note="LbCpf1"
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMRGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPRVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     misc_feature    2564..2566
                     /note="G532R"
     misc_feature    2753..2755
                     /note="K595R"
     CDS             4655..4702
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4709..4789
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /note="3xHA"
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     polyA_signal    4869..5093
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5139..5567
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5581..5910
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      5761..5896
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             5977..6771
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6945..7066
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7115..7131)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7139..7155
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7163..7193)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7208..7229
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7517..8105)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8276..9136)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9137..9241)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatggcccc aaagaagaag cggaaggtcg gtatccacgg
      961 agtcccagca gccagcaagc tggagaagtt tacaaactgc tactccctgt ctaagaccct
     1021 gaggttcaag gccatccctg tgggcaagac ccaggagaac atcgacaata agcggctgct
     1081 ggtggaggac gagaagagag ccgaggatta taagggcgtg aagaagctgc tggatcgcta
     1141 ctatctgtct tttatcaacg acgtgctgca cagcatcaag ctgaagaatc tgaacaatta
     1201 catcagcctg ttccggaaga aaaccagaac cgagaaggag aataaggagc tggagaacct
     1261 ggagatcaat ctgcggaagg agatcgccaa ggccttcaag ggcaacgagg gctacaagtc
     1321 cctgtttaag aaggatatca tcgagacaat cctgccagag ttcctggacg ataaggacga
     1381 gatcgccctg gtgaacagct tcaatggctt taccacagcc ttcaccggct tctttgataa
     1441 cagagagaat atgttttccg aggaggccaa gagcacatcc atcgccttca ggtgtatcaa
     1501 cgagaatctg acccgctaca tctctaatat ggacatcttc gagaaggtgg acgccatctt
     1561 tgataagcac gaggtgcagg agatcaagga gaagatcctg aacagcgact atgatgtgga
     1621 ggatttcttt gagggcgagt tctttaactt tgtgctgaca caggagggca tcgacgtgta
     1681 taacgccatc atcggcggct tcgtgaccga gagcggcgag aagatcaagg gcctgaacga
     1741 gtacatcaac ctgtataatc agaaaaccaa gcagaagctg cctaagttta agccactgta
     1801 taagcaggtg ctgagcgatc gggagtctct gagcttctac ggcgagggct atacatccga
     1861 tgaggaggtg ctggaggtgt ttagaaacac cctgaacaag aacagcgaga tcttcagctc
     1921 catcaagaag ctggagaagc tgttcaagaa ttttgacgag tactctagcg ccggcatctt
     1981 tgtgaagaac ggccccgcca tcagcacaat ctccaaggat atcttcggcg agtggaacgt
     2041 gatccgggac aagtggaatg ccgagtatga cgatatccac ctgaagaaga aggccgtggt
     2101 gaccgagaag tacgaggacg atcggagaaa gtccttcaag aagatcggct ccttttctct
     2161 ggagcagctg caggagtacg ccgacgccga tctgtctgtg gtggagaagc tgaaggagat
     2221 catcatccag aaggtggatg agatctacaa ggtgtatggc tcctctgaga agctgttcga
     2281 cgccgatttt gtgctggaga agagcctgaa gaagaacgac gccgtggtgg ccatcatgaa
     2341 ggacctgctg gattctgtga agagcttcga gaattacatc aaggccttct ttggcgaggg
     2401 caaggagaca aacagggacg agtccttcta tggcgatttt gtgctggcct acgacatcct
     2461 gctgaaggtg gaccacatct acgatgccat ccgcaattat gtgacccaga agccctactc
     2521 taaggataag ttcaagctgt attttcagaa ccctcagttc atgcgcggct gggacaagga
     2581 taaggagaca gactatcggg ccaccatcct gagatacggc tccaagtact atctggccat
     2641 catggataag aagtacgcca agtgcctgca gaagatcgac aaggacgatg tgaacggcaa
     2701 ttacgagaag atcaactata agctgctgcc cggccctaat aagatgctgc caagggtgtt
     2761 cttttctaag aagtggatgg cctactataa ccccagcgag gacatccaga agatctacaa
     2821 gaatggcaca ttcaagaagg gcgatatgtt taacctgaat gactgtcaca agctgatcga
     2881 cttctttaag gatagcatct cccggtatcc aaagtggtcc aatgcctacg atttcaactt
     2941 ttctgagaca gagaagtata aggacatcgc cggcttttac agagaggtgg aggagcaggg
     3001 ctataaggtg agcttcgagt ctgccagcaa gaaggaggtg gataagctgg tggaggaggg
     3061 caagctgtat atgttccaga tctataacaa ggacttttcc gataagtctc acggcacacc
     3121 caatctgcac accatgtact tcaagctgct gtttgacgag aacaatcacg gacagatcag
     3181 gctgagcgga ggagcagagc tgttcatgag gcgcgcctcc ctgaagaagg aggagctggt
     3241 ggtgcaccca gccaactccc ctatcgccaa caagaatcca gataatccca agaaaaccac
     3301 aaccctgtcc tacgacgtgt ataaggataa gaggttttct gaggaccagt acgagctgca
     3361 catcccaatc gccatcaata agtgccccaa gaacatcttc aagatcaata cagaggtgcg
     3421 cgtgctgctg aagcacgacg ataaccccta tgtgatcggc atcgataggg gcgagcgcaa
     3481 tctgctgtat atcgtggtgg tggacggcaa gggcaacatc gtggagcagt attccctgaa
     3541 cgagatcatc aacaacttca acggcatcag gatcaagaca gattaccact ctctgctgga
     3601 caagaaggag aaggagaggt tcgaggcccg ccagaactgg acctccatcg agaatatcaa
     3661 ggagctgaag gccggctata tctctcaggt ggtgcacaag atctgcgagc tggtggagaa
     3721 gtacgatgcc gtgatcgccc tggaggacct gaactctggc tttaagaata gccgcgtgaa
     3781 ggtggagaag caggtgtatc agaagttcga gaagatgctg atcgataagc tgaactacat
     3841 ggtggacaag aagtctaatc cttgtgcaac aggcggcgcc ctgaagggct atcagatcac
     3901 caataagttc gagagcttta agtccatgtc tacccagaac ggcttcatct tttacatccc
     3961 tgcctggctg acatccaaga tcgatccatc taccggcttt gtgaacctgc tgaaaaccaa
     4021 gtataccagc atcgccgatt ccaagaagtt catcagctcc tttgacagga tcatgtacgt
     4081 gcccgaggag gatctgttcg agtttgccct ggactataag aacttctctc gcacagacgc
     4141 cgattacatc aagaagtgga agctgtactc ctacggcaac cggatcagaa tcttccggaa
     4201 tcctaagaag aacaacgtgt tcgactggga ggaggtgtgc ctgaccagcg cctataagga
     4261 gctgttcaac aagtacggca tcaattatca gcagggcgat atcagagccc tgctgtgcga
     4321 gcagtccgac aaggccttct actctagctt tatggccctg atgagcctga tgctgcagat
     4381 gcggaacagc atcacaggcc gcaccgacgt ggattttctg atcagccctg tgaagaactc
     4441 cgacggcatc ttctacgata gccggaacta tgaggcccag gagaatgcca tcctgccaaa
     4501 gaacgccgac gccaatggcg cctataacat cgccagaaag gtgctgtggg ccatcggcca
     4561 gttcaagaag gccgaggacg agaagctgga taaggtgaag atcgccatct ctaacaagga
     4621 gtggctggag tacgcccaga ccagcgtgaa gcacaaaagg ccggcggcca cgaaaaaggc
     4681 cggccaggca aaaaagaaaa agggatccta cccatacgat gttccagatt acgcttatcc
     4741 ctacgacgtg cctgattatg catacccata tgatgtcccc gactatgcct aagaattctg
     4801 cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc
     4861 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4921 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4981 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     5041 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     5101 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     5161 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5221 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5281 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5341 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5401 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5461 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5521 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     5581 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5641 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5701 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5761 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5821 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     5881 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     5941 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     6001 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     6061 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     6121 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     6181 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     6241 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     6301 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     6361 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     6421 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     6481 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     6541 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     6601 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     6661 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     6721 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     6781 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     6841 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     6901 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     6961 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7021 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     7081 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7141 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7201 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7261 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7321 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7381 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7441 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7501 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7561 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7621 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7681 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7741 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7801 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7861 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7921 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7981 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8041 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8101 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8161 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8221 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8281 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8341 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8401 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8461 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8521 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8581 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8641 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8701 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8761 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8821 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8881 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8941 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9001 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9061 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9121 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9181 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9241 gcacatttcc ccgaaaagtg ccacctgacg tc
//
