LOCUS       pLL3.7m-psMEK1ti        8200 bp    DNA     circular UNA 26-FEB-2017
DEFINITION  pLLM-Arc-SMASh nHA cAU1 lenti transfer vector.
ACCESSION   urn.local...79kdmds-1b
VERSION     urn.local...79kdmds-1b
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        1..564
                     /created_by="User"
                     /label="cmv immearly promoter"
     misc_feature    1..6
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="SpeI, AhlI, BcuI"
                     /note="Geneious type: restriction site"
     enhancer        67..354
                     /created_by="User"
                     /label="CAG enhancer"
     lenti           584..764
                     /created_by="User"
                     /label="LTR"
     misc_feature    767..772
                     /Recognition_pattern="GG^CGCC"
                     /annotation_group="GGCGCC"
                     /label="NarI, Mly113I"
                     /note="Geneious type: restriction site"
     misc_feature    767..772
                     /Recognition_pattern="GGCGC^C"
                     /annotation_group="GGCGCC"
                     /label="BbeI"
                     /note="Geneious type: restriction site"
     misc_feature    767..772
                     /Recognition_pattern="GGC^GCC"
                     /annotation_group="GGCGCC"
                     /label="DinI, EgeI, EheI, SfoI"
                     /note="Geneious type: restriction site"
     misc_feature    767..772
                     /Recognition_pattern="G^GCGCC"
                     /annotation_group="GGCGCC"
                     /label="KasI, SspDI"
                     /note="Geneious type: restriction site"
     misc_feature    841..846
                     /Recognition_pattern="G^CGCGC"
                     /annotation_group="GCGCGC"
                     /label="BsePI, BssHII, PauI, PteI"
                     /note="Geneious type: restriction site"
     misc_feature    875..919
                     /created_by="User"
                     /label="PSI"
                     /note="Geneious type: lenti pkg element"
     misc_feature    complement(1237..1243)
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /annotation_group="GCTCTTC"
                     /label="SapI, BspQI, LguI, PciSI"
                     /note="Geneious type: restriction site"
     misc_feature    1275..1282
                     /Recognition_pattern="GGCCGG^CC"
                     /annotation_group="GGCCGGCC"
                     /label="FseI, RigI"
                     /note="Geneious type: restriction site"
     misc_feature    1298..1308
                     /Recognition_pattern="CCTNN^NNNAGG"
                     /annotation_group="CCTNNNNNAGG"
                     /label="EcoNI, BstENI, XagI"
                     /note="Geneious type: restriction site"
     misc_feature    1321..1326
                     /Recognition_pattern="C^AATTG"
                     /annotation_group="CAATTG"
                     /label="MfeI, MunI"
                     /note="Geneious type: restriction site"
     lenti           1435..1668
                     /created_by="User"
                     /label="RRE (rev responsive element"
     misc_feature    1705..1714
                     /Recognition_pattern="CACNN^NNGTG"
                     /annotation_group="CACNNNNGTG"
                     /label="OliI, AleI"
                     /note="Geneious type: restriction site"
     misc_feature    2065..2071
                     /Recognition_pattern="GG^GWCCC"
                     /annotation_group="GGGWCCC"
                     /label="KflI"
                     /note="Geneious type: restriction site"
     misc_feature    2357..2362
                     /Recognition_pattern="G^GGCCC"
                     /annotation_group="GGGCCC"
                     /label="Bsp120I, PspOMI"
                     /note="Geneious type: restriction site"
     misc_feature    2357..2362
                     /Recognition_pattern="GGGCC^C"
                     /annotation_group="GGGCCC"
                     /label="ApaI"
                     /note="Geneious type: restriction site"
     misc_feature    2365..2370
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    2427..2439
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /annotation_group="GAAGNNNNNNTAC"
                     /label="BarI"
                     /note="Geneious type: restriction site"
     misc_feature    2698..2705
                     /Recognition_pattern="VC^TCGAGB"
                     /annotation_group="VCTCGAGB"
                     /label="PspXI"
                     /note="Geneious type: restriction site"
     misc_feature    2698..2705
                     /Recognition_pattern="CC^TCGAGG"
                     /annotation_group="CCTCGAGG"
                     /label="AbsI"
                     /note="Geneious type: restriction site"
     misc_feature    2714..2719
                     /Recognition_pattern="AT^CGAT"
                     /annotation_group="ATCGAT"
                     /label="ClaI, BanIII, Bsa29I, BseCI, BshVI, BspDI, Bsu15I,
                     BsuTUI"
                     /note="Geneious type: restriction site"
     misc            2759..2792
                     /created_by="User"
                     /label="loxP"
     misc_feature    2813..2820
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI, CciNI"
                     /note="Geneious type: restriction site"
     misc_feature    2842..2847
                     /Recognition_pattern="ATGCA^T"
                     /annotation_group="ATGCAT"
                     /label="EcoT22I, Mph1103I, NsiI, Zsp2I"
                     /note="Geneious type: restriction site"
     promoter        2850..3357
                     /created_by="User"
                     /label="CMV prom"
     enhancer        2854..3141
                     /created_by="User"
                     /label="CAG enhancer"
     misc_feature    3452..3457
                     /Recognition_pattern="C^CTAGG"
                     /annotation_group="CCTAGG"
                     /label="AvrII, AspA2I, BlnI, XmaJI"
                     /note="Geneious type: restriction site"
     misc_feature    3458..3463
                     /Recognition_pattern="CCGC^GG"
                     /annotation_group="CCGCGG"
                     /label="SacII, Cfr42I, KspI, Sfr303I, SgrBI, SstII"
                     /note="Geneious type: restriction site"
     misc_feature    3463^3464
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    3465
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    3536..3541
                     /Recognition_pattern="T^GTACA"
                     /annotation_group="TGTACA"
                     /label="Bsp1407I, BsrGI, BstAUI"
                     /note="Geneious type: restriction site"
     misc_feature    3542..3547
                     /Original_Bases
                     /modified_by="User"
                     /label="kozak"
                     /note="Geneious type: signaling peptide"
     misc_feature    3541^3542
                     /Original_Bases="TATCCCTACGATGTGCCCGATTACGCT"
                     /label="TATCCCTACGATGTGCCCGATTACGCT"
                     /note="Geneious type: Editing History Deletion"
     tag             3551..3577
                     /Original_Bases
                     /modified_by="User"
                     /label="HA tag"
     cds             3578..3754
                     /created_by="User"
                     /label="NTD"
     linker          3761..3787
                     /created_by="User"
                     /label="GSS3"
     CDS_2           3788..>4450
                     /created_by="User"
                     /modified_by="User"
                     /label="pdDronpa1.2"
     CDS             4451..5188
                     /created_by="User"
                     /label="MEK1"
     mutation        4922..4924
                     /created_by="User"
                     /modified_by="User"
                     /label="218D"
     mutation        4934..4936
                     /created_by="User"
                     /label="222D"
     CDS_2           5189..>5860
                     /created_by="User"
                     /modified_by="User"
                     /label="pdDronpa1.2"
     mutation        5306..5308
                     /created_by="User"
                     /label="M40I"
     mutation        5366..5368
                     /created_by="User"
                     /label="V60A"
     mutation        5549..5551
                     /created_by="User"
                     /label="D121R"
     mutation        5555..5557
                     /created_by="User"
                     /label="V123T"
     mutation        5621..>5623
                     /created_by="User"
                     /modified_by="User"
                     /label="145K"
     mutation        5660..5662
                     /created_by="User"
                     /modified_by="User"
                     /label="N158I"
     CDS             5864..6133
                     /created_by="User"
                     /label="MEK1"
     misc_feature    <6137..>3541
                     /label="Digestion of pLLM_Arc_SMASh (modified) - Fragment
                     2 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    6137..6142
                     /Recognition_pattern="A^CGCGT"
                     /annotation_group="ACGCGT"
                     /label="MluI"
                     /note="Geneious type: restriction site"
     lenti           6206..6386
                     /created_by="User"
                     /modified_by="User"
                     /label="LTR"
     primer          complement(6415..6434)
                     /created_by="User"
                     /label="Temptest1"
     CDS             complement(7207..8067)
                     /created_by="User"
                     /label="AmpR"
     misc_feature    7275..7285
                     /Recognition_pattern="GACNNN^NNGTC"
                     /annotation_group="GACNNNNNGTC"
                     /label="Eam1105I, AhdI, AspEI, BmeRI, DriI"
                     /note="Geneious type: restriction site"
     misc_feature    7500..7505
                     /Recognition_pattern="TGC^GCA"
                     /annotation_group="TGCGCA"
                     /label="Acc16I, AviII, FspI, NsbI"
                     /note="Geneious type: restriction site"
     misc_feature    7758..7763
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI, AssI, BmcAI, ZrmI"
                     /note="Geneious type: restriction site"
     promoter        complement(8109..8137)
                     /created_by="User"
                     /label="Amp promoter"
ORIGIN      
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      541 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
      601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
      781 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
      841 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
      901 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
      961 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     1021 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     1081 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     1141 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     1201 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     1261 accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag atatgaggga
     1321 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1381 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1441 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1501 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1561 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1621 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1681 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1741 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1801 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1861 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1921 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1981 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2041 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2101 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2161 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2221 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2281 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2341 cagtttggtt agtaccgggc ccgctctaga gatccgacgc cgccatctct aggcccgcgc
     2401 cggccccctc gcacagactt gtgggagaag ctcggctact cccctgcccc ggttaatttg
     2461 catataatat ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca gataatctgt
     2521 tctttttaat actagctaca ttttacatga taggcttgga tttctataag agatacaaat
     2581 actaaattat tattttaaaa aacagcacaa aaggaaactc accctaactg taaagtaatt
     2641 gtgtgttttg agactataaa tatcccttgg agaaaagcct tgttaacgcg cggtgaccct
     2701 cgaggtcgac ggtatcgata agctcgcttc acgagattcc agcaggtcga gggacctaat
     2761 aacttcgtat agcatacatt atacgaagtt atattaaggg ttccaagctt aagcggccgc
     2821 gtggataacc gtattaccgc catgcatgtc gttacataac ttacggtaaa tggcccgcct
     2881 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     2941 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     3001 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     3061 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     3121 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     3181 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     3241 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     3301 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     3361 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     3421 caccgggacc gatccagcct ccggactcta gcctaggccg cgggacggat aacaatttca
     3481 cacaggaaac agctatgacc attaggccta tttaggtgac actatagaac aagtttgtac
     3541 agccaccatg tatccctacg atgtgcccga ttacgctccc aagaagaagc cgacgcccat
     3601 ccagctgaac ccgacccccg atggctcggc ggttaacggg accagctcgg ccgagaccaa
     3661 cctggaggcc ttgcagaaga agctggaggg gctggagctt gatgagcagc agcggaagcg
     3721 ccttgaggcc tttctgaccc agaagcagaa ggtgggatcc ggcagtagcg gaagcagtgg
     3781 ttcttcaagc gtgatcaagc ccgacatgaa aatcaagctg aggatggagg gtgccgtgaa
     3841 cggtcacccg tttgcgatcg agggcgtggg actgggcaag ccgttcgagg gaaagcaaag
     3901 catcgacctc aaggtgaagg agggcggacc gctgcccttc gcctacgaca ttctgaccac
     3961 tgccttctgc tacggtaatc gcgtcttcgc aaagtatccc gaaaacatcg tggattactt
     4021 taagcagagc ttcccggagg gttactcctg ggagcgatcc atgaactacg aggacggagg
     4081 gatctgcaac gcgacgaacg atataacctt ggacggcgac tgctacattt acgaaattcg
     4141 gttccgcggc accaatttcc cagcgaatgg ccctgtaatg caaaaacgca cggtaaagtg
     4201 ggagcccagc accgagaacc tgtacgtccg cgacggagtc ctgaagggag acgtgatcat
     4261 ggcgctttca ttggaaggtg gcgggcacta cagatgcgat ttcaagacga cctataaggc
     4321 gaaaaaggtg gtccaactgc cggactacca tttcgtcgac caccacatcg agatcaagag
     4381 ccacgacaaa gactatagca acgtgaatct ccacgagcat gccgaggccc acagcgagct
     4441 gcccaggcag ggagaactga aggatgatga ctttgagaag atcagtgagc tgggggccgg
     4501 caacggtgga gtggtattca aggtctccca taagccgtct ggcctagtta tggccaggaa
     4561 gctaattcat ctggagatca aacccgcaat ccggaaccag atcatccggg agctgcaggt
     4621 gctgcatgag tgcaactccc catacatcgt gggcttctat ggggctttct acagtgacgg
     4681 cgagatcagc atctgcatgg aacacatgga tggtgggtcc ttggatcaag ttctgaagaa
     4741 agctggaaga attcctgagc aaattttagg aaaagttagc attgctgtga taaaaggcct
     4801 gacatatctt cgggagaagc acaagattat gcacagagat gtcaagcctt ccaatattct
     4861 agtaaactcc cgtggagaaa tcaaactctg tgattttggg gtcagcgggc aactaattga
     4921 cgacatggcc aacgactttg taggcacaag atcatacatg tcgcccgaga gactccaggg
     4981 gactcactac tccgtgcagt cggacatctg gagcatgggg ctctctctgg tggagatggc
     5041 agttgggaga taccccatcc ctcctcctga tgccaaggaa ctggagctgc tgtttaccgg
     5101 tggatgccag gtggagggag atgcagccga aacgccaccc aggccgagga cccctgggag
     5161 gccccttagc tcatatggaa tggacagcgg cagtgtgatt aaaccagaca tgaagatcaa
     5221 gctgcgtatg gaaggcgctg taaatggaca cccgttcgcg attgaaggag ttggccttgg
     5281 gaagcctttc gagggaaaac agagtattga ccttaaagtc aaagaaggcg gacctctgcc
     5341 tttcgcctat gacatcttga caactgcttt ctgttacggc aacagggtat tcgccaaata
     5401 cccagaaaat atagtagact atttcaagca gtcgtttcct gagggctact cttgggaacg
     5461 aagcatgaat tacgaagacg ggggcatttg taacgcgaca aacgacataa ccctggatgg
     5521 tgactgttat atctatgaaa ttcgatttag aggtacgaac tttcctgcca atggtccagt
     5581 tatgcagaag aggactgtga aatgggagcc atccactgag aacttgtatg tgcgtgatgg
     5641 agtgctgaag ggtgatgtta tcatggctct gtcgcttgaa ggaggtggcc attaccgatg
     5701 tgacttcaaa actacttata aagctaagaa ggttgtccag ttgccagact atcactttgt
     5761 ggaccaccac attgagatta aaagccacga caaagattac agtaatgtta atctgcatga
     5821 gcacgccgaa gcgcattctg agctgccgag gcaggccaag cgacgtacgc ctcccatggc
     5881 aatttttgag ttgttggatt atatagtcaa tgagcctcct ccaaaattgc ccagtggagt
     5941 attcagtctg gagtttcaag attttgtgaa taaatgctta ataaagaacc ctgcagagag
     6001 agcagatcta aagcagcttc tggttcacgc tttcatcaag agatctgatg ccgaggaagt
     6061 agacttcgca ggttggctct gctccaccat tgggcttaac cagcccagca caccaaccca
     6121 tgccgctagc atctgaacgc gtgattttaa aagaaaaggg gggactggaa gggctaattc
     6181 actcccactg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     6241 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6301 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6361 ttagtcagtg tggaaaatct ctagcagcat gtgagcaaaa ggccagcaaa aggccaggaa
     6421 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6481 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6541 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6601 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6661 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6721 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6781 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6841 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6901 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6961 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7021 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7081 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7141 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7201 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7261 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7321 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7381 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7441 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7501 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7561 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7621 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7681 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7741 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7801 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7861 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7921 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7981 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8041 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8101 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8161 aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
