LOCUS       pLL3.7m-psMEK2          8248 bp    DNA     circular UNA 26-FEB-2017
DEFINITION  pLLM-Arc-SMASh nHA cAU1 lenti transfer vector.
ACCESSION   urn.local...79kdoxp-1y
VERSION     urn.local...79kdoxp-1y
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        1..564
                     /created_by="User"
                     /label="cmv immearly promoter"
     misc_feature    1..6
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="SpeI, AhlI, BcuI"
                     /note="Geneious type: restriction site"
     enhancer        67..354
                     /created_by="User"
                     /label="CAG enhancer"
     lenti           584..764
                     /created_by="User"
                     /label="LTR"
     misc_feature    767..772
                     /Recognition_pattern="GG^CGCC"
                     /annotation_group="GGCGCC"
                     /label="NarI, Mly113I"
                     /note="Geneious type: restriction site"
     misc_feature    767..772
                     /Recognition_pattern="GGCGC^C"
                     /annotation_group="GGCGCC"
                     /label="BbeI"
                     /note="Geneious type: restriction site"
     misc_feature    767..772
                     /Recognition_pattern="GGC^GCC"
                     /annotation_group="GGCGCC"
                     /label="DinI, EgeI, EheI, SfoI"
                     /note="Geneious type: restriction site"
     misc_feature    767..772
                     /Recognition_pattern="G^GCGCC"
                     /annotation_group="GGCGCC"
                     /label="KasI, SspDI"
                     /note="Geneious type: restriction site"
     misc_feature    841..846
                     /Recognition_pattern="G^CGCGC"
                     /annotation_group="GCGCGC"
                     /label="BsePI, BssHII, PauI, PteI"
                     /note="Geneious type: restriction site"
     misc_feature    875..919
                     /created_by="User"
                     /label="PSI"
                     /note="Geneious type: lenti pkg element"
     misc_feature    complement(1237..1243)
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /annotation_group="GCTCTTC"
                     /label="SapI, BspQI, LguI, PciSI"
                     /note="Geneious type: restriction site"
     misc_feature    1275..1282
                     /Recognition_pattern="GGCCGG^CC"
                     /annotation_group="GGCCGGCC"
                     /label="FseI, RigI"
                     /note="Geneious type: restriction site"
     misc_feature    1298..1308
                     /Recognition_pattern="CCTNN^NNNAGG"
                     /annotation_group="CCTNNNNNAGG"
                     /label="EcoNI, BstENI, XagI"
                     /note="Geneious type: restriction site"
     misc_feature    1321..1326
                     /Recognition_pattern="C^AATTG"
                     /annotation_group="CAATTG"
                     /label="MfeI, MunI"
                     /note="Geneious type: restriction site"
     lenti           1435..1668
                     /created_by="User"
                     /label="RRE (rev responsive element"
     misc_feature    1705..1714
                     /Recognition_pattern="CACNN^NNGTG"
                     /annotation_group="CACNNNNGTG"
                     /label="OliI, AleI"
                     /note="Geneious type: restriction site"
     misc_feature    2065..2071
                     /Recognition_pattern="GG^GWCCC"
                     /annotation_group="GGGWCCC"
                     /label="KflI"
                     /note="Geneious type: restriction site"
     misc_feature    2357..2362
                     /Recognition_pattern="G^GGCCC"
                     /annotation_group="GGGCCC"
                     /label="Bsp120I, PspOMI"
                     /note="Geneious type: restriction site"
     misc_feature    2357..2362
                     /Recognition_pattern="GGGCC^C"
                     /annotation_group="GGGCCC"
                     /label="ApaI"
                     /note="Geneious type: restriction site"
     misc_feature    2365..2370
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    2427..2439
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /annotation_group="GAAGNNNNNNTAC"
                     /label="BarI"
                     /note="Geneious type: restriction site"
     misc_feature    2698..2705
                     /Recognition_pattern="VC^TCGAGB"
                     /annotation_group="VCTCGAGB"
                     /label="PspXI"
                     /note="Geneious type: restriction site"
     misc_feature    2698..2705
                     /Recognition_pattern="CC^TCGAGG"
                     /annotation_group="CCTCGAGG"
                     /label="AbsI"
                     /note="Geneious type: restriction site"
     misc_feature    2714..2719
                     /Recognition_pattern="AT^CGAT"
                     /annotation_group="ATCGAT"
                     /label="ClaI, BanIII, Bsa29I, BseCI, BshVI, BspDI, Bsu15I,
                     BsuTUI"
                     /note="Geneious type: restriction site"
     misc            2759..2792
                     /created_by="User"
                     /label="loxP"
     misc_feature    2813..2820
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI, CciNI"
                     /note="Geneious type: restriction site"
     misc_feature    2842..2847
                     /Recognition_pattern="ATGCA^T"
                     /annotation_group="ATGCAT"
                     /label="EcoT22I, Mph1103I, NsiI, Zsp2I"
                     /note="Geneious type: restriction site"
     promoter        2850..3357
                     /created_by="User"
                     /label="CMV prom"
     enhancer        2854..3141
                     /created_by="User"
                     /label="CAG enhancer"
     misc_feature    3452..3457
                     /Recognition_pattern="C^CTAGG"
                     /annotation_group="CCTAGG"
                     /label="AvrII, AspA2I, BlnI, XmaJI"
                     /note="Geneious type: restriction site"
     misc_feature    3458..3463
                     /Recognition_pattern="CCGC^GG"
                     /annotation_group="CCGCGG"
                     /label="SacII, Cfr42I, KspI, Sfr303I, SgrBI, SstII"
                     /note="Geneious type: restriction site"
     misc_feature    3463^3464
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    3465
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     sequencing      3466..3485
                     /created_by="User"
                     /label="oQE31-III"
     misc_feature    3536..3541
                     /Recognition_pattern="T^GTACA"
                     /annotation_group="TGTACA"
                     /label="Bsp1407I, BsrGI, BstAUI"
                     /note="Geneious type: restriction site"
     misc_feature    3542..3547
                     /Original_Bases
                     /modified_by="User"
                     /label="kozak"
                     /note="Geneious type: signaling peptide"
     tag             3551..3577
                     /Original_Bases
                     /modified_by="User"
                     /label="HA tag"
     cds             3578..3766
                     /created_by="User"
                     /label="NTD"
     linker          3773..3799
                     /created_by="User"
                     /label="GSS3"
     CDS_2           3800..4468
                     /created_by="User"
                     /modified_by="User"
                     /label="pdDronpa1.2"
     primer          4229..4231
                     /created_by="User"
                     /label=145
     cds             4490..5233
                     /created_by="User"
                     /label="MEK2 64-462"
     CDS_2           5240..>5911
                     /created_by="User"
                     /modified_by="User"
                     /label="pdDronpa1.2"
     mutation        5357..5359
                     /created_by="User"
                     /label="M40I"
     mutation        5417..5419
                     /created_by="User"
                     /label="V60A"
     mutation        5600..5602
                     /created_by="User"
                     /label="D121R"
     mutation        5606..5608
                     /created_by="User"
                     /label="V123T"
     mutation        5672..>5673
                     /created_by="User"
                     /modified_by="User"
                     /label="145N"
     mutation        5711..5713
                     /created_by="User"
                     /modified_by="User"
                     /label="N158I"
     cds             5918..6181
                     /created_by="User"
                     /modified_by="User"
                     /label="MEK2 ct"
     misc_feature    <6185..>3541
                     /label="Digestion of pLLM_Arc_SMASh (modified) - Fragment
                     2 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    6185..6190
                     /Recognition_pattern="A^CGCGT"
                     /annotation_group="ACGCGT"
                     /label="MluI"
                     /note="Geneious type: restriction site"
     lenti           6254..6434
                     /created_by="User"
                     /modified_by="User"
                     /label="LTR"
     sequencing      complement(6463..6482)
                     /created_by="User"
                     /label="Temptest1"
     CDS             complement(7255..8115)
                     /created_by="User"
                     /label="AmpR"
     misc_feature    7323..7333
                     /Recognition_pattern="GACNNN^NNGTC"
                     /annotation_group="GACNNNNNGTC"
                     /label="Eam1105I, AhdI, AspEI, BmeRI, DriI"
                     /note="Geneious type: restriction site"
     misc_feature    7548..7553
                     /Recognition_pattern="TGC^GCA"
                     /annotation_group="TGCGCA"
                     /label="Acc16I, AviII, FspI, NsbI"
                     /note="Geneious type: restriction site"
     misc_feature    7806..7811
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI, AssI, BmcAI, ZrmI"
                     /note="Geneious type: restriction site"
     promoter        complement(8157..8185)
                     /created_by="User"
                     /label="Amp promoter"
ORIGIN      
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      541 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
      601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
      781 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
      841 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
      901 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
      961 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     1021 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     1081 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     1141 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     1201 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     1261 accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag atatgaggga
     1321 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1381 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1441 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1501 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1561 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1621 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1681 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1741 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1801 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1861 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1921 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1981 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2041 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2101 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2161 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2221 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2281 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2341 cagtttggtt agtaccgggc ccgctctaga gatccgacgc cgccatctct aggcccgcgc
     2401 cggccccctc gcacagactt gtgggagaag ctcggctact cccctgcccc ggttaatttg
     2461 catataatat ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca gataatctgt
     2521 tctttttaat actagctaca ttttacatga taggcttgga tttctataag agatacaaat
     2581 actaaattat tattttaaaa aacagcacaa aaggaaactc accctaactg taaagtaatt
     2641 gtgtgttttg agactataaa tatcccttgg agaaaagcct tgttaacgcg cggtgaccct
     2701 cgaggtcgac ggtatcgata agctcgcttc acgagattcc agcaggtcga gggacctaat
     2761 aacttcgtat agcatacatt atacgaagtt atattaaggg ttccaagctt aagcggccgc
     2821 gtggataacc gtattaccgc catgcatgtc gttacataac ttacggtaaa tggcccgcct
     2881 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     2941 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     3001 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     3061 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     3121 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     3181 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     3241 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     3301 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     3361 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     3421 caccgggacc gatccagcct ccggactcta gcctaggccg cgggacggat aacaatttca
     3481 cacaggaaac agctatgacc attaggccta tttaggtgac actatagaac aagtttgtac
     3541 agccaccatg tatccctacg atgtgcccga ttacgctctt gccaggagga agcctgtgct
     3601 gcccgctctt acaattaatc ctaccattgc cgagggacca agccctacca gcgaaggcgc
     3661 atccgaagct aatctggtgg acctgcagaa gaaacttgaa gaacttgagc ttgatgagca
     3721 gcagaaaaag aggctggagg cgttcctcac acagaaggca aaagtgggat ccggcagtag
     3781 cggaagcagt ggttcttcaa gcgtgatcaa gcccgacatg aaaatcaagc tgaggatgga
     3841 gggtgccgtg aacggtcacc cgtttgcgat cgagggcgtg ggactgggca agccgttcga
     3901 gggaaagcaa agcatcgacc tcaaggtgaa ggagggcgga ccgctgccct tcgcctacga
     3961 cattctgacc actgccttct gctacggtaa tcgcgtcttc gcaaagtatc ccgaaaacat
     4021 cgtggattac tttaagcaga gcttcccgga gggttactcc tgggagcgat ccatgaacta
     4081 cgaggacgga gggatctgca acgcgacgaa cgatataacc ttggacggcg actgctacat
     4141 ttacgaaatt cggttccgcg gcaccaattt cccagcgaat ggccctgtaa tgcaaaaacg
     4201 cacggtaaag tgggagccca gcaccgagaa cctgtacgtc cgcgacggag tcctgaaggg
     4261 agacgtgatc atggcgcttt cattggaagg tggcgggcac tacagatgcg atttcaagac
     4321 gacctataag gcgaaaaagg tggtccaact gccggactac catttcgtcg accaccacat
     4381 cgagatcaag agccacgaca aagactatag caacgtgaat ctccacgagc atgccgaggc
     4441 ccacagcgag ctgcccaggc aggccaaggg ccttaagggt tccggtaccg gggagctgaa
     4501 agatgatgat tttgagagaa tatcagagct tggcgcaggt aatggcggcg tggtaactaa
     4561 ggtgcagcac cgcccatcag gactcattat ggctagaaag ctcattcacc ttgagataaa
     4621 gcctgctata cgcaaccaga taattcggga gctgcaggtc ctgcacgagt gtaattcacc
     4681 ctacattgtt ggcttctacg gggcatttta ctcagacgga gagatttcta tatgcatgga
     4741 gcacatggac ggaggaagcc tggaccaggt cctgaaggaa gcaaagagga ttcccgagga
     4801 gatcctgggc aaggtgagca ttgctgtgct gcgcgggttg gcatacctgc gggaaaagca
     4861 ccagatcatg catcgggacg tcaaacctag caacatcttg gtgaatagtc ggggcgaaat
     4921 taaactttgt gatttcggtg tgtccggcca gttgattgac gatatggcta atgatttcgt
     4981 cggcacccgc tcctacatgg ctccggaaag gctccaggga acgcactaca gcgtgcagtc
     5041 agacatctgg tccatggggc tgagcctcgt ggaattggcg gtaggcagat accctatacc
     5101 accacctgat gcaaaagagc tggaggccat cttcggcaga cccgtggttg acggggagga
     5161 gggtgaacca cactccatta gcccaagacc tcgaccacca gggaggcctg taagtgggca
     5221 cggaatggac agcaccggtg gcagtgtgat taaaccagac atgaagatca agctgcgtat
     5281 ggaaggcgct gtaaatggac acccgttcgc gattgaagga gttggccttg ggaagccttt
     5341 cgagggaaaa cagagtattg accttaaagt caaagaaggc ggacctctgc ctttcgccta
     5401 tgacatcttg acaactgctt tctgttacgg caacagggta ttcgccaaat acccagaaaa
     5461 tatagtagac tatttcaagc agtcgtttcc tgagggctac tcttgggaac gaagcatgaa
     5521 ttacgaagac gggggcattt gtaacgcgac aaacgacata accctggatg gtgactgtta
     5581 tatctatgaa attcgattta gaggtacgaa ctttcctgcc aatggtccag ttatgcagaa
     5641 gaggactgtg aaatgggagc catccactga gaacttgtat gtgcgtgatg gagtgctgaa
     5701 gggtgatgtt atcatggctc tgtcgcttga aggaggtggc cattaccgat gtgacttcaa
     5761 aactacttat aaagctaaga aggttgtcca gttgccagac tatcactttg tggaccacca
     5821 cattgagatt aaaagccacg acaaagatta cagtaatgtt aatctgcatg agcacgccga
     5881 agcgcattct gagctgccga ggcaggccaa gcgtacgagg cccgccatgg ctatctttga
     5941 actgctggat tacatcgtga acgagcctcc acccaagctg cccaacggtg ttttcacacc
     6001 cgacttccag gaattcgtca ataaatgcct gatcaaaaat ccagcagaga gagctgacct
     6061 caagatgctg acaaaccaca cgtttattaa aaggtccgaa gttgaggaag tcgatttcgc
     6121 tggctggctg tgcaagacgc tcaggctgaa tcagcccgga actcccacta gaacagcagt
     6181 ttgaacgcgt gattttaaaa gaaaaggggg gactggaagg gctaattcac tcccactgct
     6241 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6301 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6361 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6421 gaaaatctct agcagcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6481 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6541 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6601 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6661 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6721 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6781 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6841 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6901 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6961 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7021 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7081 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7141 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7201 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7261 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7321 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7381 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7441 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7501 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7561 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7621 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7681 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7741 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7801 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7861 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7921 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7981 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8041 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8101 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8161 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8221 cacatttccc cgaaaagtgc cacctgac
//
