LOCUS       pLL3.7m-psRaf1          8014 bp    DNA     circular UNA 26-FEB-2017
DEFINITION  pLLM-Arc-SMASh nHA cAU1 lenti transfer vector.
ACCESSION   urn.local...79kdigz-r
VERSION     urn.local...79kdigz-r
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        1..564
                     /created_by="User"
                     /label="cmv immearly promoter"
     misc_feature    1..6
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="SpeI, AhlI, BcuI"
                     /note="Geneious type: restriction site"
     enhancer        67..354
                     /created_by="User"
                     /label="CAG enhancer"
     lenti           584..764
                     /created_by="User"
                     /label="LTR"
     misc_feature    767..772
                     /Recognition_pattern="GG^CGCC"
                     /annotation_group="GGCGCC"
                     /label="NarI, Mly113I"
                     /note="Geneious type: restriction site"
     misc_feature    767..772
                     /Recognition_pattern="GGCGC^C"
                     /annotation_group="GGCGCC"
                     /label="BbeI"
                     /note="Geneious type: restriction site"
     misc_feature    767..772
                     /Recognition_pattern="GGC^GCC"
                     /annotation_group="GGCGCC"
                     /label="DinI, EgeI, EheI, SfoI"
                     /note="Geneious type: restriction site"
     misc_feature    767..772
                     /Recognition_pattern="G^GCGCC"
                     /annotation_group="GGCGCC"
                     /label="KasI, SspDI"
                     /note="Geneious type: restriction site"
     misc_feature    841..846
                     /Recognition_pattern="G^CGCGC"
                     /annotation_group="GCGCGC"
                     /label="BsePI, BssHII, PauI, PteI"
                     /note="Geneious type: restriction site"
     misc_feature    875..919
                     /created_by="User"
                     /label="PSI"
                     /note="Geneious type: lenti pkg element"
     misc_feature    complement(1237..1243)
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /annotation_group="GCTCTTC"
                     /label="SapI, BspQI, LguI, PciSI"
                     /note="Geneious type: restriction site"
     misc_feature    1275..1282
                     /Recognition_pattern="GGCCGG^CC"
                     /annotation_group="GGCCGGCC"
                     /label="FseI, RigI"
                     /note="Geneious type: restriction site"
     misc_feature    1298..1308
                     /Recognition_pattern="CCTNN^NNNAGG"
                     /annotation_group="CCTNNNNNAGG"
                     /label="EcoNI, BstENI, XagI"
                     /note="Geneious type: restriction site"
     misc_feature    1321..1326
                     /Recognition_pattern="C^AATTG"
                     /annotation_group="CAATTG"
                     /label="MfeI, MunI"
                     /note="Geneious type: restriction site"
     lenti           1435..1668
                     /created_by="User"
                     /label="RRE (rev responsive element"
     misc_feature    1705..1714
                     /Recognition_pattern="CACNN^NNGTG"
                     /annotation_group="CACNNNNGTG"
                     /label="OliI, AleI"
                     /note="Geneious type: restriction site"
     misc_feature    2065..2071
                     /Recognition_pattern="GG^GWCCC"
                     /annotation_group="GGGWCCC"
                     /label="KflI"
                     /note="Geneious type: restriction site"
     misc_feature    2357..2362
                     /Recognition_pattern="G^GGCCC"
                     /annotation_group="GGGCCC"
                     /label="Bsp120I, PspOMI"
                     /note="Geneious type: restriction site"
     misc_feature    2357..2362
                     /Recognition_pattern="GGGCC^C"
                     /annotation_group="GGGCCC"
                     /label="ApaI"
                     /note="Geneious type: restriction site"
     misc_feature    2365..2370
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    2427..2439
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /annotation_group="GAAGNNNNNNTAC"
                     /label="BarI"
                     /note="Geneious type: restriction site"
     misc_feature    2698..2705
                     /Recognition_pattern="VC^TCGAGB"
                     /annotation_group="VCTCGAGB"
                     /label="PspXI"
                     /note="Geneious type: restriction site"
     misc_feature    2698..2705
                     /Recognition_pattern="CC^TCGAGG"
                     /annotation_group="CCTCGAGG"
                     /label="AbsI"
                     /note="Geneious type: restriction site"
     misc_feature    2714..2719
                     /Recognition_pattern="AT^CGAT"
                     /annotation_group="ATCGAT"
                     /label="ClaI, BanIII, Bsa29I, BseCI, BshVI, BspDI, Bsu15I,
                     BsuTUI"
                     /note="Geneious type: restriction site"
     misc            2759..2792
                     /created_by="User"
                     /label="loxP"
     misc_feature    2813..2820
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI, CciNI"
                     /note="Geneious type: restriction site"
     misc_feature    2842..2847
                     /Recognition_pattern="ATGCA^T"
                     /annotation_group="ATGCAT"
                     /label="EcoT22I, Mph1103I, NsiI, Zsp2I"
                     /note="Geneious type: restriction site"
     promoter        2850..3357
                     /created_by="User"
                     /label="CMV prom"
     enhancer        2854..3141
                     /created_by="User"
                     /label="CAG enhancer"
     misc_feature    3452..3457
                     /Recognition_pattern="C^CTAGG"
                     /annotation_group="CCTAGG"
                     /label="AvrII, AspA2I, BlnI, XmaJI"
                     /note="Geneious type: restriction site"
     misc_feature    3458..3463
                     /Recognition_pattern="CCGC^GG"
                     /annotation_group="CCGCGG"
                     /label="SacII, Cfr42I, KspI, Sfr303I, SgrBI, SstII"
                     /note="Geneious type: restriction site"
     misc_feature    3463^3464
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    3465
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    3536..3541
                     /Recognition_pattern="T^GTACA"
                     /annotation_group="TGTACA"
                     /label="Bsp1407I, BsrGI, BstAUI"
                     /note="Geneious type: restriction site"
     misc_feature    3542..3547
                     /Original_Bases
                     /modified_by="User"
                     /label="kozak"
                     /note="Geneious type: signaling peptide"
     misc_feature    3541^3542
                     /Original_Bases="TATCCCTACGATGTGCCCGATTACGCT"
                     /label="TATCCCTACGATGTGCCCGATTACGCT"
                     /note="Geneious type: Editing History Deletion"
     tag             3551..3577
                     /Original_Bases
                     /modified_by="User"
                     /label="HA tag"
     CDS_2           3578..4246
                     /created_by="User"
                     /modified_by="User"
                     /label="pdDronpa1"
     cds             4262..4900
                     /created_by="User"
                     /label="Raf-Nterm"
     CDS_2           4907..>5578
                     /created_by="User"
                     /modified_by="User"
                     /label="pdDronpa1 145K"
     mutation        5024..5026
                     /created_by="User"
                     /label="M40I"
     mutation        5084..5086
                     /created_by="User"
                     /label="V60A"
     mutation        5267..5269
                     /created_by="User"
                     /label="D121R"
     mutation        5273..5275
                     /created_by="User"
                     /label="V123T"
     mutation        5339..>5341
                     /created_by="User"
                     /modified_by="User"
                     /label="145K"
     misc_feature    5378..5379
                     /Original_Bases="AA"
                     /label="AA"
                     /note="Geneious type: Editing History Replacement"
     mutation        5378..5380
                     /created_by="User"
                     /label="N158V"
     cds             5585..5872
                     /created_by="User"
                     /label="Raf-C-term"
     cds             5888..5947
                     /created_by="User"
                     /label="CAAX"
     misc_feature    <5951..>3541
                     /label="Digestion of pLLM_Arc_SMASh (modified) - Fragment
                     2 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    5951..5956
                     /Recognition_pattern="A^CGCGT"
                     /annotation_group="ACGCGT"
                     /label="MluI"
                     /note="Geneious type: restriction site"
     lenti           6020..6200
                     /created_by="User"
                     /modified_by="User"
                     /label="LTR"
     CDS             complement(7021..7881)
                     /created_by="User"
                     /label="AmpR"
     misc_feature    7089..7099
                     /Recognition_pattern="GACNNN^NNGTC"
                     /annotation_group="GACNNNNNGTC"
                     /label="Eam1105I, AhdI, AspEI, BmeRI, DriI"
                     /note="Geneious type: restriction site"
     misc_feature    7314..7319
                     /Recognition_pattern="TGC^GCA"
                     /annotation_group="TGCGCA"
                     /label="Acc16I, AviII, FspI, NsbI"
                     /note="Geneious type: restriction site"
     misc_feature    7572..7577
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI, AssI, BmcAI, ZrmI"
                     /note="Geneious type: restriction site"
     promoter        complement(7923..7951)
                     /created_by="User"
                     /label="Amp promoter"
ORIGIN      
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      541 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
      601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
      781 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
      841 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
      901 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
      961 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     1021 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     1081 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     1141 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     1201 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     1261 accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag atatgaggga
     1321 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1381 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1441 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1501 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1561 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1621 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1681 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1741 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1801 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1861 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1921 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1981 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2041 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2101 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2161 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2221 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2281 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2341 cagtttggtt agtaccgggc ccgctctaga gatccgacgc cgccatctct aggcccgcgc
     2401 cggccccctc gcacagactt gtgggagaag ctcggctact cccctgcccc ggttaatttg
     2461 catataatat ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca gataatctgt
     2521 tctttttaat actagctaca ttttacatga taggcttgga tttctataag agatacaaat
     2581 actaaattat tattttaaaa aacagcacaa aaggaaactc accctaactg taaagtaatt
     2641 gtgtgttttg agactataaa tatcccttgg agaaaagcct tgttaacgcg cggtgaccct
     2701 cgaggtcgac ggtatcgata agctcgcttc acgagattcc agcaggtcga gggacctaat
     2761 aacttcgtat agcatacatt atacgaagtt atattaaggg ttccaagctt aagcggccgc
     2821 gtggataacc gtattaccgc catgcatgtc gttacataac ttacggtaaa tggcccgcct
     2881 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     2941 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     3001 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     3061 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     3121 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     3181 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     3241 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     3301 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     3361 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     3421 caccgggacc gatccagcct ccggactcta gcctaggccg cgggacggat aacaatttca
     3481 cacaggaaac agctatgacc attaggccta tttaggtgac actatagaac aagtttgtac
     3541 agccaccatg tatccctacg atgtgcccga ttacgctagc gtgatcaagc ccgacatgaa
     3601 aatcaagctg aggatggagg gtgccgtgaa cggtcacccg tttgcgatcg agggcgtggg
     3661 actgggcaag ccgttcgagg gaaagcaaag catcgacctc aaggtgaagg agggcggacc
     3721 gctgcccttc gcctacgaca ttctgaccac tgccttctgc tacggtaatc gcgtcttcgc
     3781 aaagtatccc gaaaacatcg tggattactt taagcagagc ttcccggagg gttactcctg
     3841 ggagcgatcc atgaactacg aggacggagg gatctgcaac gcgacgaacg atataacctt
     3901 ggacggcgac tgctacattt acgaaattcg gttccgcggc accaatttcc cagcgaatgg
     3961 ccctgtaatg caaaaacgca cggtaaagtg ggagcccagc accgagaacc tgtacgtccg
     4021 cgacggagtc ctgaagggag acgtggtcat ggcgctttca ttggaaggtg gcgggcacta
     4081 cagatgcgat ttcaagacga cctataaggc gaaaaaggtg gtccaactgc cggactacca
     4141 tttcgtcgac caccacatcg agatcaagag ccacgacaaa gactatagca acgtgaatct
     4201 ccacgagcat gccgaggccc acagcgagct gcccaggcag gccaagggcc ttaagggatc
     4261 ctattattgg gaaatagaag ccagtgaagt gatgctgtcc actcggattg ggtcaggctc
     4321 ttttggaact gtttataagg gtaaatggca cggagatgtt gcagtaaaga tcctaaaggt
     4381 tgtcgaccca accccagagc aattccaggc cttcaggaat gaggtggctg ttctgcgcaa
     4441 aacacggcat gtgaacattc tgcttttcat ggggtacatg acaaaggaca acctggcaat
     4501 tgtgacccag tggtgcgagg gcagcagcct ctacaaacac ctgcatgtcc aggagaccaa
     4561 gtttcagatg ttccagctaa ttgacattgc ccggcagacg gctcagggaa tggactattt
     4621 gcatgcaaag aacatcatcc atagagacat gaaatccaac aatatatttc tccatgaagg
     4681 cttaacagtg aaaattggag attttggttt ggcaacagta aagtcacgct ggagtggttc
     4741 tcagcaggtt gaacaaccta ctggctctgt cctctggatg gccccagagg tgatccgaat
     4801 gcaggataac aacccattca gtttccagtc ggatgtctac tcctatggca tcgtattgta
     4861 tgaactgatg acgggggagc ttccttattc tcacatcaac accggtggca gtgtgattaa
     4921 accagacatg aagatcaagc tgcgtatgga aggcgctgta aatggacacc cgttcgcgat
     4981 tgaaggagtt ggccttggga agcctttcga gggaaaacag agtattgacc ttaaagtcaa
     5041 agaaggcgga cctctgcctt tcgcctatga catcttgaca actgctttct gttacggcaa
     5101 cagggtattc gccaaatacc cagaaaatat agtagactat ttcaagcagt cgtttcctga
     5161 gggctactct tgggaacgaa gcatgaatta cgaagacggg ggcatttgta acgcgacaaa
     5221 cgacataacc ctggatggtg actgttatat ctatgaaatt cgatttagag gtacgaactt
     5281 tcctgccaat ggtccagtta tgcagaagag gactgtgaaa tgggagccat ccactgagaa
     5341 attgtatgtg cgtgatggag tgctgaaggg tgatgttgtc atggctctgt cgcttgaagg
     5401 aggtggccat taccgatgtg acttcaaaac tacttataaa gctaagaagg ttgtccagtt
     5461 gccagactat cactttgtgg accaccacat tgagattaaa agccacgaca aagattacag
     5521 taatgttaat ctgcatgagc acgccgaagc gcattctgag ctgccgaggc aggccaagcg
     5581 tacgaaccga gatcagatca tcttcatggt gggccgagga tatgcctccc cagatcttag
     5641 taagctatat aagaactgcc ccaaagcaat gaagaggctg gtagctgact gtgtgaagaa
     5701 agtaaaggag gagaggcctc tttttcccca gatcctgtct tccattgagc tgctccaaca
     5761 ctctctaccg aagatcaacc ggagcgcttc cgagccatcc ttgcatcggg cagcccacac
     5821 tgaggatatc aatgcttgca cgctgaccac gtccccgagg ctgcctgtct tcgctagcga
     5881 attcggcaag atgagcaagg acggcaagaa gaaaaagaag aagtccaaga ctaagtgcgt
     5941 gatcatgtga acgcgtgatt ttaaaagaaa aggggggact ggaagggcta attcactccc
     6001 actgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6061 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6121 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6181 agtgtggaaa atctctagca gcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6241 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6301 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6361 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6421 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6481 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6541 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6601 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6661 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6721 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6781 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6841 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6901 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6961 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7021 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7081 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7141 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7201 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7261 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7321 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7381 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7441 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7501 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7561 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7621 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7681 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7741 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7801 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7861 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7921 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7981 tccgcgcaca tttccccgaa aagtgccacc tgac
//
