LOCUS Exported 8794 bp ds-DNA circular SYN 28-FEB-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCaMKII_mCh_thrombin_GluA1_CIBN_MKp391 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8794) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Feb 28, 2017 from SnapGene 3.1.3 http://www.snapgene.com COMMENT Cloned by SacII/BamHI-Got lucky making this clone-there were twoBamHI sites! Insertion and orientation confirmed by sequencing andfunctional studies FEATURES Location/Qualifiers source 1..8794 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 10..1301 /label=a-CaMKII(1.3) /note="a-CaMKII(1.3)" misc_feature 1355..1456 /label=Signal pep./linker /note="Signal pep./linker" misc_feature 1457..2161 /label=mCherry /note="mCherry" misc_feature 2222..4882 /label=ORF /note="ORF" misc_feature 4913..4939 /label=linker sequence /note="linker sequence" misc_feature 4940..5452 /label=CIBN(1) /note="CIBN(1)" misc_feature 4961..5430 /label=CIBN /note="CIBN" misc_feature 5431..5458 /label=cibn_Cterm /note="cibn_Cterm" ORIGIN 1 tagttattat aacattatgg ccttaggtca cttcatctcc atggggttct tcttctgatt 61 ttctagaaaa tgagatgggg gtgcagagag cttcctcagt gacctgccca gggtcacatc 121 agaaatgtca gagctagaac ttgaactcag attactaatc ttaaattcca tgccttgggg 181 gcatgcaagt acgatataca gaaggagtga actcattagg gcagatgacc aatgagttta 241 ggaaagaaga gtccagggca gggtacatct acaccacccg cccagccctg ggtgagtcca 301 gccacgttca cctcattata gttgcctctc tccagtccta ccttgacggg aagcacaagc 361 agaaactggg acaggagccc caggagacca aatcttcatg gtccctctgg gaggatgggt 421 ggggagagct gtggcagagg cctcaggagg ggccctgctg ctcagtggtg acagataggg 481 gtgagaaagc agacagagtc attccgtcag cattctgggt ctgtttggta cttcttctca 541 cgctaaggtg gcggtgtgat atgcacaatg gctaaaaagc agggagagct ggaaagaaac 601 aaggacagag acagaggcca agtcaaccag accaattccc agaggaagca aagaaaccat 661 tacagagact acaaggggga agggaaggag agatgaatta gcttcccctg taaaccttag 721 aacccagctg ttgccagggc aacggggcaa tacctgtctc ttcagaggag atgaagttgc 781 cagggtaact acatcctgtc tttctcaagg accatcccag aatgtggcac ccactagccg 841 ttaccatagc aactgcctct ttgccccact taatcccatc ccgtctgtta aaagggccct 901 atagttggag gtgggggagg taggaagagc gatgatcact tgtggactaa gtttgttcgc 961 atccccttct ccaaccccct cagtacatca ccctggggga acagggtcca cttgctcctg 1021 ggcccacaca gtcctgcagt attgtgtata taaggccagg gcaaagagga gcaggtttta 1081 aagtgaaagg caggcaggtg ttggggaggc agttaccggg gcaacgggaa cagggcgttt 1141 cggaggtggt tgccatgggg acctggatgc tgacgaaggc tcgcgaggct gtgagcagcc 1201 acagtgccct gctcagaagc cccaagctcg tcagtcaagc cggttctccg tttgcactca 1261 ggagcacggg caggcgagtg gcccctagtt ctgggggcag cgggggatcc ccgggtaccg 1321 ggtacaagtc cggactcaga tctcgagctc caccatgccg tacatctttg cctttttctg 1381 caccggtttt ctaggtgcgg ttgtgggtgc caatgcgcga ggtggaggcg gtgggggagg 1441 gggtggagga acgcgtgtga gcaagggcga ggaggataac atggccatca tcaaggagtt 1501 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg 1561 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg 1621 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc 1681 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt 1741 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc 1801 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc 1861 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta 1921 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg 1981 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg 2041 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt 2101 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa 2161 gctggttccg cgtggatcca cgcgtggagg tggagggggc ggaggtggcg ggggttcgcg 2221 cttccccaac aatatccaga taggggggtt atttccaaac caacaatcac aggaacatgc 2281 ggcttttagg tttgctttgt cacaactcac ggagcccccc aagctgcttc cccagatcga 2341 tattgtgaac atcagcgaca gctttgagat gacttaccgt ttctgttccc agttctccaa 2401 aggagtctat gccatctttg gattttatga acgaaggact gtcaacatgc tgacctcctt 2461 ctgtggggcc ctccatgtgt gcttcattac tccaagtttt cctgttgaca catccaatca 2521 atttgtcctt cagctacgcc cggaactaca ggaagctctc attagcatta tcgaccatta 2581 caagtggcaa acctttgtct acatttatga tgctgaccgg ggcctgtcag tcctgcagag 2641 agtcttggat acagccgcag agaagaactg gcaggtaacg gctgtcaaca ttctgacaac 2701 caccgaggaa ggataccgga tgctctttca ggacctggag aagaaaaagg agaggctggt 2761 ggtggttgac tgtgaatcag aacgcctcaa cgccatcctg ggccagatcg tgaagctaga 2821 aaagaatggc atcgggtacc actacatcct cgccaatctg ggcttcatgg acattgactt 2881 aaataagttc aaggagagcg gagccaatgt gacaggtttc cagctggtga actacacaga 2941 cacgatccca gccagaatca tgcagcaatg gaggacaagt gactcccgag accataccag 3001 ggtggactgg aagaggccaa agtacacttc tgctctcacc tatgatggtg tcaaggtgat 3061 ggctgaggcc ttccaaagcc tgcggaggca gaggattgac atatcccgcc gggggaatgc 3121 tggggactgt ctggctaacc cagctgtgcc ctggggtcaa gggatcgaca tccagagagc 3181 cctgcagcag gtgcgcttcg aaggtttgac aggaaatgtg cagttcaacg agaaagggcg 3241 ccggaccaat tacaccctcc acgtgatcga aatgaaacat gatggaatcc gaaagattgg 3301 ttactggaat gaagacgata aatttgtccc cgcagccacc gacgctcagg ctggagggga 3361 caactcaagc gtccagaata ggacctacat cgtcactact atcctcgaag atccttacgt 3421 gatgcttaaa aagaatgcca accagtttga gggcaatgac cgctatgagg gctactgtgt 3481 ggagctggct gcagagatcg ccaagcacgt gggctactcc taccgacttg agattgtcag 3541 cgacggcaaa tatggagccc gggatcccga cacaaaggct tggaatggca tggtgggaga 3601 actggtctat ggaagagcag acgtggctgt ggctcccttg accataacct tggtccggga 3661 ggaagtcatc gacttctcca agccattcat gagtttggga atctccatta tgattaagaa 3721 gccacagaag tccaagccag gtgtcttctc ctttcttgac cctttggcct atgagatctg 3781 gatgtgtata gtgtttgcct acattggagt gagcgtcgtc ctcttcctgg tcagccgttt 3841 cagcccctac gaatggcaca gcgaagagtt tgaagaggga cgagaccaga caaccagtga 3901 ccagtcaaat gagtttggca tattcaacag cctgtggttc tccctggggg ccttcatgca 3961 gcaaggatgt gacatttccc ccaggtccct gtccggacgc atcgtcggcg gcgtctggtg 4021 gttcttcact ttgatcatca tctcctcgta cacagccaac ctggctgcct tcctgactgt 4081 ggagaggatg gtgtctccca ttgagagtgc agaggacctg gcaaagcaga cggaaattgc 4141 ttatgggaca ttggaagcag gctccactaa ggagttcttc aggagatcta aaatcgctgt 4201 gtttgagaag atgtggacat acatgaagtc tgcagaacca tccgtgtttg ttcggaccac 4261 agaggaaggc atgatcagag tgagaaaatc taaaggcaaa tacgcctacc tcctggagtc 4321 caccatgaat gagtatattg agcaacgaaa gccctgtgac accatgaaag tgggaggtaa 4381 cttggattcc aaaggctatg gcattgcgac acccaagggg tccgccctga gaaatccagt 4441 aaacctggca gtgttaaaac tgaacgagca ggggcttttg gacaaattga aaaacaaatg 4501 gtggtacgac aagggcgagt gcggcagcgg gggaggtgac tccaaggaca agaccagcgc 4561 tttgagcctc agcaatgtgg caggcgtgtt ctacatcctg attggagggc tgggactggc 4621 catgctggtt gccttaatcg agttctgcta caaatcccgt agcgagtcga agcggatgaa 4681 gggtttctgt ttgatcccac agcaatccat caatgaagcc atacggacat cgaccctccc 4741 ccggaacagt ggggcaggag ccagcggagg aggcggcagt ggagagaatg gccgggtggt 4801 cagccaggac ttccccaagt ccatgcaatc cattccctgc atgagtcaca gttcagggat 4861 gcccttggga gccacaggat tggggaattc tgcagtcgac ggtaccgcgg atagtgctgg 4921 tagtgctggt agtgctggta atggagctat aggaggtgac cttttgctca attttcctga 4981 catgtcggtc ctagagcgcc aaagggctca cctcaagtac ctcaatccca cctttgattc 5041 tcctctcgcc ggcttctttg ccgattcttc aatgattacc ggcggcgaga tggacagcta 5101 tctttcgact gccggtttga atcttccgat gatgtacggt gagacgacgg tggaaggtga 5161 ttcaagactc tcaatttcgc cggaaacgac gcttgggact ggaaatttca aggcagcgaa 5221 gtttgataca gagactaagg attgtaatga ggcggcgaag aagatgacga tgaacagaga 5281 tgacctagta gaagaaggag aagaagagaa gtcgaaaata acagagcaaa acaatgggag 5341 cacaaaaagc atcaagaaga tgaaacacaa agccaagaaa gaagagaaca atttctctaa 5401 tgattcatct aaagtgacga aggaattgga gaaaacggat tatattcatt aaggatccac 5461 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt 5521 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt 5581 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 5641 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 5701 ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa 5761 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat 5821 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg 5881 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac 5941 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta 6001 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag 6061 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg 6121 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc 6181 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 6241 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg 6301 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 6361 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 6421 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 6481 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 6541 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 6601 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg 6661 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt 6721 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 6781 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 6841 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg 6901 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 6961 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 7021 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 7081 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 7141 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 7201 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 7261 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 7321 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 7381 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 7441 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 7501 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 7561 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 7621 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg 7681 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac 7741 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt 7801 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc 7861 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc 7921 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt 7981 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 8041 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 8101 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 8161 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 8221 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 8281 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 8341 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 8401 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 8461 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 8521 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 8581 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 8641 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 8701 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 8761 tgattctgtg gataaccgta ttaccgccat gcat //