LOCUS       Exported                8794 bp ds-DNA     circular SYN 28-FEB-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCaMKII_mCh_thrombin_GluA1_CIBN_MKp391
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 28, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
COMMENT     Cloned by SacII/BamHI-Got lucky making this clone-there were 
            twoBamHI sites! Insertion and orientation confirmed by sequencing 
            andfunctional studies
FEATURES             Location/Qualifiers
     source          1..8794
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    10..1301
                     /label=a-CaMKII(1.3)
                     /note="a-CaMKII(1.3)"
     misc_feature    1355..1456
                     /label=Signal pep./linker
                     /note="Signal pep./linker"
     misc_feature    1457..2161
                     /label=mCherry
                     /note="mCherry"
     misc_feature    2222..4882
                     /label=ORF
                     /note="ORF"
     misc_feature    4913..4939
                     /label=linker sequence
                     /note="linker sequence"
     misc_feature    4940..5452
                     /label=CIBN(1)
                     /note="CIBN(1)"
     misc_feature    4961..5430
                     /label=CIBN
                     /note="CIBN"
     misc_feature    5431..5458
                     /label=cibn_Cterm
                     /note="cibn_Cterm"
ORIGIN
        1 tagttattat aacattatgg ccttaggtca cttcatctcc atggggttct tcttctgatt
       61 ttctagaaaa tgagatgggg gtgcagagag cttcctcagt gacctgccca gggtcacatc
      121 agaaatgtca gagctagaac ttgaactcag attactaatc ttaaattcca tgccttgggg
      181 gcatgcaagt acgatataca gaaggagtga actcattagg gcagatgacc aatgagttta
      241 ggaaagaaga gtccagggca gggtacatct acaccacccg cccagccctg ggtgagtcca
      301 gccacgttca cctcattata gttgcctctc tccagtccta ccttgacggg aagcacaagc
      361 agaaactggg acaggagccc caggagacca aatcttcatg gtccctctgg gaggatgggt
      421 ggggagagct gtggcagagg cctcaggagg ggccctgctg ctcagtggtg acagataggg
      481 gtgagaaagc agacagagtc attccgtcag cattctgggt ctgtttggta cttcttctca
      541 cgctaaggtg gcggtgtgat atgcacaatg gctaaaaagc agggagagct ggaaagaaac
      601 aaggacagag acagaggcca agtcaaccag accaattccc agaggaagca aagaaaccat
      661 tacagagact acaaggggga agggaaggag agatgaatta gcttcccctg taaaccttag
      721 aacccagctg ttgccagggc aacggggcaa tacctgtctc ttcagaggag atgaagttgc
      781 cagggtaact acatcctgtc tttctcaagg accatcccag aatgtggcac ccactagccg
      841 ttaccatagc aactgcctct ttgccccact taatcccatc ccgtctgtta aaagggccct
      901 atagttggag gtgggggagg taggaagagc gatgatcact tgtggactaa gtttgttcgc
      961 atccccttct ccaaccccct cagtacatca ccctggggga acagggtcca cttgctcctg
     1021 ggcccacaca gtcctgcagt attgtgtata taaggccagg gcaaagagga gcaggtttta
     1081 aagtgaaagg caggcaggtg ttggggaggc agttaccggg gcaacgggaa cagggcgttt
     1141 cggaggtggt tgccatgggg acctggatgc tgacgaaggc tcgcgaggct gtgagcagcc
     1201 acagtgccct gctcagaagc cccaagctcg tcagtcaagc cggttctccg tttgcactca
     1261 ggagcacggg caggcgagtg gcccctagtt ctgggggcag cgggggatcc ccgggtaccg
     1321 ggtacaagtc cggactcaga tctcgagctc caccatgccg tacatctttg cctttttctg
     1381 caccggtttt ctaggtgcgg ttgtgggtgc caatgcgcga ggtggaggcg gtgggggagg
     1441 gggtggagga acgcgtgtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     1501 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     1561 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1621 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     1681 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     1741 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     1801 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     1861 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     1921 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     1981 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     2041 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     2101 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     2161 gctggttccg cgtggatcca cgcgtggagg tggagggggc ggaggtggcg ggggttcgcg
     2221 cttccccaac aatatccaga taggggggtt atttccaaac caacaatcac aggaacatgc
     2281 ggcttttagg tttgctttgt cacaactcac ggagcccccc aagctgcttc cccagatcga
     2341 tattgtgaac atcagcgaca gctttgagat gacttaccgt ttctgttccc agttctccaa
     2401 aggagtctat gccatctttg gattttatga acgaaggact gtcaacatgc tgacctcctt
     2461 ctgtggggcc ctccatgtgt gcttcattac tccaagtttt cctgttgaca catccaatca
     2521 atttgtcctt cagctacgcc cggaactaca ggaagctctc attagcatta tcgaccatta
     2581 caagtggcaa acctttgtct acatttatga tgctgaccgg ggcctgtcag tcctgcagag
     2641 agtcttggat acagccgcag agaagaactg gcaggtaacg gctgtcaaca ttctgacaac
     2701 caccgaggaa ggataccgga tgctctttca ggacctggag aagaaaaagg agaggctggt
     2761 ggtggttgac tgtgaatcag aacgcctcaa cgccatcctg ggccagatcg tgaagctaga
     2821 aaagaatggc atcgggtacc actacatcct cgccaatctg ggcttcatgg acattgactt
     2881 aaataagttc aaggagagcg gagccaatgt gacaggtttc cagctggtga actacacaga
     2941 cacgatccca gccagaatca tgcagcaatg gaggacaagt gactcccgag accataccag
     3001 ggtggactgg aagaggccaa agtacacttc tgctctcacc tatgatggtg tcaaggtgat
     3061 ggctgaggcc ttccaaagcc tgcggaggca gaggattgac atatcccgcc gggggaatgc
     3121 tggggactgt ctggctaacc cagctgtgcc ctggggtcaa gggatcgaca tccagagagc
     3181 cctgcagcag gtgcgcttcg aaggtttgac aggaaatgtg cagttcaacg agaaagggcg
     3241 ccggaccaat tacaccctcc acgtgatcga aatgaaacat gatggaatcc gaaagattgg
     3301 ttactggaat gaagacgata aatttgtccc cgcagccacc gacgctcagg ctggagggga
     3361 caactcaagc gtccagaata ggacctacat cgtcactact atcctcgaag atccttacgt
     3421 gatgcttaaa aagaatgcca accagtttga gggcaatgac cgctatgagg gctactgtgt
     3481 ggagctggct gcagagatcg ccaagcacgt gggctactcc taccgacttg agattgtcag
     3541 cgacggcaaa tatggagccc gggatcccga cacaaaggct tggaatggca tggtgggaga
     3601 actggtctat ggaagagcag acgtggctgt ggctcccttg accataacct tggtccggga
     3661 ggaagtcatc gacttctcca agccattcat gagtttggga atctccatta tgattaagaa
     3721 gccacagaag tccaagccag gtgtcttctc ctttcttgac cctttggcct atgagatctg
     3781 gatgtgtata gtgtttgcct acattggagt gagcgtcgtc ctcttcctgg tcagccgttt
     3841 cagcccctac gaatggcaca gcgaagagtt tgaagaggga cgagaccaga caaccagtga
     3901 ccagtcaaat gagtttggca tattcaacag cctgtggttc tccctggggg ccttcatgca
     3961 gcaaggatgt gacatttccc ccaggtccct gtccggacgc atcgtcggcg gcgtctggtg
     4021 gttcttcact ttgatcatca tctcctcgta cacagccaac ctggctgcct tcctgactgt
     4081 ggagaggatg gtgtctccca ttgagagtgc agaggacctg gcaaagcaga cggaaattgc
     4141 ttatgggaca ttggaagcag gctccactaa ggagttcttc aggagatcta aaatcgctgt
     4201 gtttgagaag atgtggacat acatgaagtc tgcagaacca tccgtgtttg ttcggaccac
     4261 agaggaaggc atgatcagag tgagaaaatc taaaggcaaa tacgcctacc tcctggagtc
     4321 caccatgaat gagtatattg agcaacgaaa gccctgtgac accatgaaag tgggaggtaa
     4381 cttggattcc aaaggctatg gcattgcgac acccaagggg tccgccctga gaaatccagt
     4441 aaacctggca gtgttaaaac tgaacgagca ggggcttttg gacaaattga aaaacaaatg
     4501 gtggtacgac aagggcgagt gcggcagcgg gggaggtgac tccaaggaca agaccagcgc
     4561 tttgagcctc agcaatgtgg caggcgtgtt ctacatcctg attggagggc tgggactggc
     4621 catgctggtt gccttaatcg agttctgcta caaatcccgt agcgagtcga agcggatgaa
     4681 gggtttctgt ttgatcccac agcaatccat caatgaagcc atacggacat cgaccctccc
     4741 ccggaacagt ggggcaggag ccagcggagg aggcggcagt ggagagaatg gccgggtggt
     4801 cagccaggac ttccccaagt ccatgcaatc cattccctgc atgagtcaca gttcagggat
     4861 gcccttggga gccacaggat tggggaattc tgcagtcgac ggtaccgcgg atagtgctgg
     4921 tagtgctggt agtgctggta atggagctat aggaggtgac cttttgctca attttcctga
     4981 catgtcggtc ctagagcgcc aaagggctca cctcaagtac ctcaatccca cctttgattc
     5041 tcctctcgcc ggcttctttg ccgattcttc aatgattacc ggcggcgaga tggacagcta
     5101 tctttcgact gccggtttga atcttccgat gatgtacggt gagacgacgg tggaaggtga
     5161 ttcaagactc tcaatttcgc cggaaacgac gcttgggact ggaaatttca aggcagcgaa
     5221 gtttgataca gagactaagg attgtaatga ggcggcgaag aagatgacga tgaacagaga
     5281 tgacctagta gaagaaggag aagaagagaa gtcgaaaata acagagcaaa acaatgggag
     5341 cacaaaaagc atcaagaaga tgaaacacaa agccaagaaa gaagagaaca atttctctaa
     5401 tgattcatct aaagtgacga aggaattgga gaaaacggat tatattcatt aaggatccac
     5461 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
     5521 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     5581 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5641 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5701 ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     5761 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     5821 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     5881 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     5941 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     6001 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     6061 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     6121 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     6181 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6241 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     6301 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     6361 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     6421 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6481 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6541 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     6601 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     6661 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     6721 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     6781 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     6841 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     6901 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     6961 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     7021 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     7081 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     7141 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     7201 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     7261 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     7321 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     7381 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     7441 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     7501 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     7561 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     7621 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     7681 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     7741 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     7801 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     7861 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     7921 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     7981 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8041 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8101 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8161 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8221 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8281 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8341 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8401 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8461 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8521 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8581 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8641 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8701 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8761 tgattctgtg gataaccgta ttaccgccat gcat
//
