LOCUS       pLenti-PRSx8-eAr        9769 bp    DNA     circular     03-MAR-2017
SOURCE
  ORGANISM
KEYWORDS
COMMENT     This file is created by Vector NTI















            http://www.invitrogen.com/















            VNTDATE|617807446|















            VNTDBDATE|617807446|















            LSOWNER|















            VNTNAME|FCK (1.3)-eArchT 3.0-EYFP-W|















            VNTAUTHORNAME|Charu Ramakrishnan|















            VNTOAUTHORNAME|Feng Zhang|
FEATURES             Location/Qualifiers
     LTR             5574..5754
                     /label="3' LTR"
     misc_feature    4451..5059
                     /label="WPRE"
     LTR             835..1015
                     /label="5'LTR"
     misc_binding    1691..1895
                     /label="RRE"
     enhancer        3636..3695
                     /label="TS"
     enhancer        4410..4430
                     /label="ER export"
     misc_feature    3696..4409
                     /label="EYFP"
     misc_feature    2883..3626
                     /label="Arch-T"
     misc_feature    2598..2867
                     /label="PRSx8"
     -10_signal      2656..2858
                     /label="Seq. #1"
     -10_signal      1771..2791
                     /label="Seq. #2"
     -10_signal      4436..5410
                     /label="Seq. #3"
     misc_binding    5755..5760
                     /label="ApaI"
     misc_binding    4437..4442
                     /label="EcoRI"
     misc_binding    5183..5188
                     /label="KpnI"
     misc_binding    4402..4407
                     /label="BsrGI"
     misc_binding    2856..2861
                     /label="XbaI"
     misc_binding    15..20
                     /label="BglII"
     misc_binding    854..859
                     /label="BglII"
     misc_binding    5219..5224
                     /label="BglII"
     misc_binding    5593..5598
                     /label="BglII"
     misc_binding    5065..5070
                     /label="XhoI"
     misc_binding    2862..2867
                     /label="AgeI"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 
     2581 agatccagtt tggttaataa ttaaaaacgc gtataagctt ccgctagaca aatgtgatta 
     2641 cccccgctag acaaatgtga ttacccgcgc tagacaaatg tgattacccc gctagacaaa 
     2701 tgtgattacc ccccgctaga caaatgtgat tacccccgct agacaaatgt gattacccgc 
     2761 gctagacaaa tgtgattacc ccgctagaca aatgtgatta cccccgacca gggcataaat 
     2821 ggccaggtgg gaccagagag ctcaccccag ccgactctag aaccggtggg ggatccgcca 
     2881 ccatggaccc aattgcactg caggcggggt atgacctcct cggtgacggg aggcccgaga 
     2941 cactctggct gggcatcggg acgcttctca tgctcatcgg aacgttttac ttcatcgtca 
     3001 aaggatgggg tgtaacagac aaggaagcgc gcgagtacta ctcgatcact attcttgtgc 
     3061 cgggcatcgc ctcagcggcg tatttgagca tgttctttgg catcgggctg accgaagtga 
     3121 cggtagcggg agaggtcttg gatatctact atgcgcgcta cgcagactgg ctgtttacaa 
     3181 ctccgctcct tttgcttgat cttgctctgt tggcaaaagt agatagggtg tcgattggga 
     3241 cgctggtggg agtagatgcc cttatgattg tcacgggatt gattggggca ttgtcgcaca 
     3301 cgcctctcgc ccgatattcg tggtggttgt tttccaccat ctgcatgatc gtggtcctct 
     3361 acttcttggc gacgagcctt cgggctgcgg cgaaggagag aggacccgag gtcgcgtcca 
     3421 cattcaacac cttgactgcc ctggtgctgg tgctctggac cgcgtatccc atcttgtgga 
     3481 ttatcggaac tgaaggtgcc ggggtcgtcg gtctgggaat cgaaacactc ctcttcatgg 
     3541 tgttggacgt aacagccaag gtcggatttg gtttcatctt gctgcggtca agagcaattc 
     3601 ttggggatac ggaagccccg gagcctgcgg ccgccaagag caggatcacc agcgagggcg 
     3661 agtacatccc cctggaccag atcgacatca acgtggtgag caagggcgag gagctgttca 
     3721 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 
     3781 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 
     3841 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc 
     3901 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 
     3961 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 
     4021 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 
     4081 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 
     4141 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 
     4201 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 
     4261 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca 
     4321 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 
     4381 tcactctcgg catggacgag ctgtacaagt tctgctacga gaacgaggtg taatgagaat 
     4441 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg 
     4501 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 
     4561 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 
     4621 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 
     4681 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 
     4741 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 
     4801 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat 
     4861 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 
     4921 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 
     4981 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 
     5041 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta 
     5101 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg 
     5161 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag 
     5221 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 
     5281 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga 
     5341 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag 
     5401 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac 
     5461 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg 
     5521 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc 
     5581 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
     5641 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
     5701 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc 
     5761 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc 
     5821 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 
     5881 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 
     5941 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg 
     6001 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg 
     6061 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 
     6121 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 
     6181 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 
     6241 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 
     6301 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 
     6361 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 
     6421 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 
     6481 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 
     6541 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 
     6601 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 
     6661 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 
     6721 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat 
     6781 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag 
     6841 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta 
     6901 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc 
     6961 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct 
     7021 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac 
     7081 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg 
     7141 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg 
     7201 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc 
     7261 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt 
     7321 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 
     7381 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 
     7441 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 
     7501 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 
     7561 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 
     7621 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 
     7681 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 
     7741 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 
     7801 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 
     7861 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
     7921 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
     7981 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
     8041 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
     8101 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
     8161 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 
     8221 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     8281 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     8341 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     8401 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 
     8461 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
     8521 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
     8581 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
     8641 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
     8701 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
     8761 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 
     8821 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 
     8881 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 
     8941 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 
     9001 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
     9061 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 
     9121 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 
     9181 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 
     9241 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 
     9301 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
     9361 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 
     9421 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 
     9481 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 
     9541 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 
     9601 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
     9661 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 
     9721 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac 
//