LOCUS       PRSX8ChR2EYFP           9865 bp    DNA     circular     03-MAR-2017
SOURCE
  ORGANISM
KEYWORDS
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              (CStringList) 23265040 23265904 (CObList)))
FEATURES             Location/Qualifiers
     LTR             5670..5850
                     /label="3' LTR"
     misc_feature    4547..5155
                     /label="WPRE"
     LTR             835..1015
                     /label="5'LTR"
     misc_binding    1691..1895
                     /label="RRE"
     exon            3816..4532
                     /label="EYFP"
     exon            2871..3800
                     /label="hChR2"
     misc_binding    6905..6910
                     /label="StuI"
     misc_binding    591..596
                     /label="SnaBI"
     misc_binding    252..257
                     /label="SpeI"
     misc_binding    5857..5864
                     /label="PmeI"
     misc_binding    2594..2601
                     /label="PacI"
     misc_binding    4533..4538
                     /label="EcoRI"
     misc_binding    5279..5284
                     /label="KpnI"
     misc_binding    4522..4527
                     /label="BsrGI"
     misc_binding    2853..2858
                     /label="XbaI"
     misc_binding    9312..9317
                     /label="PvuI"
     misc_binding    8052..8057
                     /label="PciI"
     misc_binding    9165..9170
                     /label="FspI"
     misc_binding    2859..2864
                     /label="AgeI"
     promoter        2618..2834
                     /label="PRSX8"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 
     2581 agatccagtt tggttaatta aaaacgcgta taagcttccg ctagacaaat gtgattaccc 
     2641 ccgctagaca aatgtgatta cccgcgctag acaaatgtga ttaccccgct agacaaatgt 
     2701 gattaccccc cgctagacaa atgtgattac ccccgctaga caaatgtgat tacccgcgct 
     2761 agacaaatgt gattaccccg ctagacaaat gtgattaccc ccgaccaggg cataaatggc 
     2821 caggtgggac cagagagctc accccagccg actctagaac cggtgccacc atggactatg 
     2881 gcggcgcttt gtctgccgtc ggacgcgaac ttttgttcgt tactaatcct gtggtggtga 
     2941 acgggtccgt cctggtccct gaggatcaat gttactgtgc cggatggatt gaatctcgcg 
     3001 gcacgaacgg cgctcagacc gcgtcaaatg tcctgcagtg gcttgcagca ggattcagca 
     3061 ttttgctgct gatgttctat gcctaccaaa cctggaaatc tacatgcggc tgggaggaga 
     3121 tctatgtgtg cgccattgaa atggttaagg tgattctcga gttctttttt gagtttaaga 
     3181 atccctctat gctctacctt gccacaggac accgggtgca gtggctgcgc tatgcagagt 
     3241 ggctgctcac ttgtcctgtc atccttatcc acctgagcaa cctcaccggc ctgagcaacg 
     3301 actacagcag gagaaccatg ggactccttg tctcagacat cgggactatc gtgtgggggg 
     3361 ctaccagcgc catggcaacc ggctatgtta aagtcatctt cttttgtctt ggattgtgct 
     3421 atggcgcgaa cacatttttt cacgccgcca aagcatatat cgagggttat catactgtgc 
     3481 caaagggtcg gtgccgccag gtcgtgaccg gcatggcatg gctgtttttc gtgagctggg 
     3541 gtatgttccc aattctcttc attttggggc ccgaaggttt tggcgtcctg agcgtctatg 
     3601 gctccaccgt aggtcacacg attattgatc tgatgagtaa aaattgttgg gggttgttgg 
     3661 gacactacct gcgcgtcctg atccacgagc acatattgat tcacggagat atccgcaaaa 
     3721 ccaccaaact gaacatcggc ggaacggaga tcgaggtcga gactctcgtc gaagacgaag 
     3781 ccgaggccgg agccgtgcca gcggccgcca ccatggtgag caagggcgag gagctgttca 
     3841 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 
     3901 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 
     3961 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc 
     4021 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 
     4081 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 
     4141 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 
     4201 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 
     4261 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 
     4321 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 
     4381 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca 
     4441 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 
     4501 tcactctcgg catggacgag ctgtacaagt aagaattcga tatcaagctt atcgataatc 
     4561 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 
     4621 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 
     4681 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 
     4741 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 
     4801 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 
     4861 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 
     4921 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct 
     4981 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 
     5041 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 
     5101 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac 
     5161 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg 
     5221 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg 
     5281 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 
     5341 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga 
     5401 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca 
     5461 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag 
     5521 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg 
     5581 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca 
     5641 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct 
     5701 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 
     5761 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 
     5821 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg 
     5881 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 
     5941 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 
     6001 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 
     6061 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 
     6121 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 
     6181 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 
     6241 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 
     6301 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 
     6361 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 
     6421 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 
     6481 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 
     6541 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc 
     6601 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 
     6661 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 
     6721 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 
     6781 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     6841 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt 
     6901 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg 
     6961 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa 
     7021 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga 
     7081 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga 
     7141 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga 
     7201 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta 
     7261 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac 
     7321 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg 
     7381 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc 
     7441 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 
     7501 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 
     7561 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 
     7621 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc 
     7681 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 
     7741 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 
     7801 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 
     7861 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 
     7921 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 
     7981 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 
     8041 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 
     8101 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 
     8161 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 
     8221 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 
     8281 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 
     8341 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 
     8401 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 
     8461 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 
     8521 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 
     8581 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 
     8641 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 
     8701 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 
     8761 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 
     8821 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 
     8881 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 
     8941 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 
     9001 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 
     9061 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 
     9121 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 
     9181 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 
     9241 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 
     9301 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 
     9361 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 
     9421 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 
     9481 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 
     9541 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 
     9601 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 
     9661 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 
     9721 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 
     9781 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 
     9841 atttccccga aaagtgccac ctgac 
//