LOCUS pBW390_pCAG_NLS_ 5859 bp ds-DNA circular 06-MAR-2017 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 3845..3854 /label=mcherry primer annealing /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4574..4708 /label=SV40 ori /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3817..4353 /label=Rabbit Beta Globin pA /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2753..3808 /label=NLS-iCre /ApEinfo_fwdcolor=#ff3985 /ApEinfo_revcolor=#ff3985 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 862..966 /label=AmpR Promoter /ApEinfo_fwdcolor=#333333 /ApEinfo_revcolor=#333333 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1011..2717 /label=CAG Promoter /ApEinfo_fwdcolor=#008080 /ApEinfo_revcolor=#008080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4728..4862 /label=SV40pA /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2744..2752 /label=Kozak /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1..861 /label=AmpR /ApEinfo_fwdcolor=#999999 /ApEinfo_revcolor=#999999 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5101..5689 /label=ori /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT 61 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC 121 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA 181 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 241 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 301 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT 361 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC 421 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT 481 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 541 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 601 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT 661 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC 721 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG 781 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 841 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 901 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 961 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGGgTCGACA TTGATTATTG ACTAGTTATT 1021 AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT 1081 AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA 1141 TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG 1201 AgTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC 1261 CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT 1321 TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTCG 1381 AGGTGAGCCC CACGTTCTGC TTCACTCTCC CCATCTCCCC CCCCTCCCCA CCCCCAATTT 1441 TGTATTTATT TATTTTTTAA TTATTTTGTG CAGCGATGGG GGCGGGGGGG GGGGGGGCGC 1501 GCGCCAGGCG GGGCGGGGCG GGGCGAGGGG CGGGGCGGGG CGAGGCGGAa AGGTGCGGCG 1561 GCAGCCAATC AGAGCGGCGC GCTCCGAAAG TTTCCTTTTA TGGCGAGGCG GCGGCGGCGG 1621 CGGCCCTATA AAAAGCGAAG CGCGCGGCGG GCGGGAGTCG CTGCGTTGCC TTCGCCCCGT 1681 GCCCCGCTCC GCGCCGCCTC GCGCCGCCCG CCCCGGCTCT GACTGACCGC GTTACTCCCA 1741 CAGGTGAGCG GGCGGGACGG CCCTTCTCCT CCGGGCTGTA ATTAGCGCTT GGTTTAATGA 1801 CGGCTCGTTT CTTTTCTGTG GCTGCGTGAA AGCCTTAAAG GGCTCCGGGA GGGCCCTTTG 1861 TGCGGGGGGG AGCGGCTCGG GGGGTGCGTG CGTGTGTGTG TGCGTGGGGA GCGCCGCGTG 1921 CGGCtCCGCG CTGCCCGGCG GCTGTGAGCG CTGCGGGCGC GGCGCGGGGC TTTGTGCGCT 1981 CCGCagTGTG CGCGAGGGGA GCGCGGCCGG GGGCGGTGCC CCGCGGTGCG gGGGGGGCTG 2041 CGAGGGGAAC AAAGGCTGCG TGCGGGGTGT GTGCGTGGGG GGGTGAGCAG GGGGTGTGGG 2101 CGCGGCGGTC GGGCTGTAAC CCCCCCCTGC ACCCCCCTCC CCGAGTTGCT GAGCACGGCC 2161 CGGCTTCGGG TGCGGGGCTC CGTaCGGGGC GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG 2221 GGTGGCGGCA GGTGGGGGTG CCGGGCGGGG CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG 2281 GGGAGGGGCG CGGCGGCCCC cGGAGCGCCG GCGGCTGTCG AGGCGCGGCG AGCCGCAGCC 2341 ATTGCCTTTT ATGGTAATCG TGCGAGAGGG CGCAGGGACT TCCTTTGTCC CAAATCTGtG 2401 CGGAGCCGAA ATCTGGGAGG CGCCGCCGCA CCCCCTCTAG CGGGCGCGGG gCGAAGCGGT 2461 GCGGCGCCGG CAGGAAGGAA ATGGGCGGGG AGGGCCTTCG TGCGTCGCCG CGCCGCCGTC 2521 CCCTTCTCCc TCTCCAGCCT CGGGGCTGtC CGCgGGGGGA CGGCTGCCTT CGGGGGGGAC 2581 GGGGCAGGGC GGGGTTCGGC TTCTGGCGTG TGACCGGCGG CTCTAGAGCC TCTGCTAACC 2641 ATGTTCATGC CTTCTTCTTT TTCCTACAGC TCCTGGGCAA CGTGCTGGTT ATTGTGCTGT 2701 CTCATCATTT TGGCAAAGAA TTGATTAATT CGAGCGAACG CGTgccgcca ccatggtgcc 2761 caagaagaag aggaaagtct ccaacctgct gactgtgcac caaaacctgc ctgccctccc 2821 tgtggatgcc acctctgatg aagtcaggaa gaacctgatg gacatgttca gggacaggca 2881 ggccttctct gaacacacct ggaagatgct cctgtctgtg tgcagatcct gggctgcctg 2941 gtgcaagctg aacaacagga aatggttccc tgctgaacct gaggatgtga gggactacct 3001 cctgtacctg caagccagag gcctggctgt gaagaccatc caacagcacc tgggccagct 3061 caacatgctg cacaggagat ctggcctgcc tcgcccttct gactccaatg ctgtgtccct 3121 ggtgatgagg agaatcagaa aggagaatgt ggatgctggg gagagagcca agcaggccct 3181 ggcctttgaa cgcactgact ttgaccaagt cagatccctg atggagaact ctgacagatg 3241 ccaggacatc aggaacctgg ccttcctggg cattgcctac aacaccctgc tgcgcattgc 3301 cgaaattgcc agaatcagag tgaaggacat ctcccgcacc gatggtggga gaatgctgat 3361 ccacattggc aggaccaaga ccctggtgtc cacagctggt gtggagaagg ccctgtccct 3421 gggggttacc aagctggtgg agagatggat ctctgtgtct ggtgtggctg atgaccccaa 3481 caactacctg ttctgccggg tcagaaagaa tggtgtggct gccccttctg ccacctccca 3541 actgtccacc cgggccctgg aagggatctt tgaggccacc caccgcctga tctatggtgc 3601 caaggatgac tctgggcaga gatacctggc ctggtctggc cactctgcca gagtgggtgc 3661 tgccagggac atggccaggg ctggtgtgtc catccctgaa atcatgcagg ctggtggctg 3721 gaccaatgtg aacattgtga tgaactacat cagaaacctg gactctgaga ctggggccat 3781 ggtgaggctg ctcgaggatg gggactgaGC GGCCGCACTC CTCAGGTGCA GGCTGCCTAT 3841 CAGAAGGTGG TGGCTGGTGT GGCCAATGCC CTGGCTCACA AATACCACTG AGATCTTTTT 3901 CCCTCTGCCA AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC TTCTGGCTAA 3961 TAAAGGAAAT TTATTTTCAT TGCAATAGTG TGTTGGAATT TTTTGTGTCT CTCACTCGGA 4021 AGGACATATG GGAGGGCAAA TCATTTAAAA CATCAGAATG AGTATTTGGT TTAGAGTTTG 4081 GCAACATATG CCCATATGCT GGCTGCCATG AACAAAGGTT GGCTATAAAG AGGTCATCAG 4141 TATATGAAAC AGCCCCCTGC TGTCCATTCC TTATTCCATA GAAAAGCCTT GACTTGAGGT 4201 TAGATTTTTT TTATATTTTG TTTTGTGTTA TTTTTTTCTT TAACATCCCT AAAATTTTCC 4261 TTACATGTTT TACTAGCCAG ATTTTTCCTC CTCTCCTGAC TACTCCCAGT CATAGCTGTC 4321 CCTCTTCTCT TATGGAGATC CCTCGACCTG CAGCCCAAGC TTGGCGTAAT CATGGTCATA 4381 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG 4441 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG 4501 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CGGATCCGCA TCTCAATTAG 4561 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 4621 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 4681 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTAAC TTGTTTATTG 4741 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 4801 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA 4861 TCCGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC 4921 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC 4981 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 5041 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 5101 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 5161 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 5221 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 5281 CGTGGCGCTT TCTCAtaGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 5341 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 5401 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 5461 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 5521 TAACTACGGC TACACTAGAA GaACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 5581 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 5641 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 5701 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 5761 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 5821 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAG //