LOCUS       Exported                6697 bp ds-DNA     circular SYN 16-MAR-2017
DEFINITION  synthetic circular DNA
ACCESSION   C-His-3607
VERSION     .
KEYWORDS    pPJ187
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 16, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(505..1320)
                     /codon_start=1
                     /note="KanR2"
                     /note="ORF frame 3"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1426..2045
                     /note="pBR322_origin"
     misc_feature    complement(3460..4551)
                     /note="lacI"
     promoter        4929..4947
                     /note="T7_promoter"
     misc_feature    4947..4974
                     /note="lacO"
     misc_feature    4992..5008
                     /note="T7_transl_en_RBS"
     gene            5016..6482
                     /note="kuste3607"
     misc_feature    6486..6503
                     /note="6xHis"
     terminator      6514..6642
                     /note="T7_terminator"
     misc_feature    complement(6556..6574)
                     /note="T7_Terminal_primer"
     rep_origin      6671..280
                     /note="f1_origin"
ORIGIN
        1 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
       61 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      121 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
      181 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
      241 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
      301 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
      361 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcaggtggc
      421 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      481 atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa
      541 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
      601 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
      661 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
      721 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc
      781 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
      841 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa
      901 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
      961 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat
     1021 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     1081 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
     1141 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
     1201 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     1261 atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat
     1321 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg accaaaatcc
     1381 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1441 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1501 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1561 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     1621 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1681 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1741 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1801 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1861 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1921 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1981 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2041 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2101 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2161 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     2221 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct
     2281 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     2341 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     2401 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     2461 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
     2521 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
     2581 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     2641 tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg
     2701 atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg
     2761 gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact
     2821 cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag
     2881 catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga
     2941 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
     3001 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
     3061 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggg
     3121 gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg
     3181 acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc
     3241 gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt
     3301 cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc
     3361 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt
     3421 gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3481 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3541 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc
     3601 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag
     3661 gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc
     3721 gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg
     3781 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc
     3841 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc
     3901 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg
     3961 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
     4021 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
     4081 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
     4141 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
     4201 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
     4261 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
     4321 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
     4381 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
     4441 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac
     4501 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg
     4561 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc
     4621 cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     4681 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
     4741 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     4801 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     4861 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
     4921 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
     4981 aataattttg tttaacttta agaaggagat atacaatgag tagcaccaaa ccaattaaac
     5041 cacatattca aagtaccaag attgacacgc tgtatccgag ttacgatgtg attgttattg
     5101 gtgccggtat cgccggtctg atttgtgccg ccttcctggc caaaagtggt aagaaagccc
     5161 tgctgattga gcaacatttc attccaggtg gctactgtac cagctttaaa cgtaagggtt
     5221 tcctgtttga tgcggcggtt catcatattg gcggttgcgg taaatggagc gtggtgggtc
     5281 gttgtttcaa aatgctgggc atcgaaatgg acttttacca tctggaccca atggataatc
     5341 tgatcttccc aacgtttagc attgaaattc cagccgatct ggatgaatac ctgctgcgcc
     5401 tgcaaaagcg ttacccacat gaaaaggata acattaagtg gttttttcaa gactttatcc
     5461 gcctgtaccg cgccaccttc cataatgaga aaagtcaaat catcgagaaa tacaaagata
     5521 tgacgtacgg catgatgctg gatcatttct ttgatgatag tgaactgaaa atggttctga
     5581 gcggccagtg gggttacgtg ggtctggccc cgagcaaggc cagcgccatt gccatgagcc
     5641 aaatgatgat taactatctg aaagatggtg ccttttttcc aaagggtggt acccaagaat
     5701 ttgccaatgc catttttcgt aagtttatcg atttcggcgg tcatgttatg ctgagtagta
     5761 aagtgaagaa aatcctgaag aagggcccaa gtgttaatgg tgtgaaactg aatgacgata
     5821 aggaaattca tagcagtgtt gtggtgagca atatcgatgc gcgtcagacc ttttatcatc
     5881 tgctgaacga cggtaatgat gtggatgccg tttacctgaa aaacattgag ggtatgaaag
     5941 aaagttgtag tttttttctg ctgtacctgg gtatcggtaa aggcattgac ctgaatacgc
     6001 tgaagcgcgg cttctacctg agtggtagca atttcgacag catcgacagt gaatggatgt
     6061 atattagtgt gccaaccagt atttgtcaag cgctgagtcc acaaaataag caaattatca
     6121 gtatcgtggt gtacaagaaa gaagagttct acaaaaatgt gaccgattgg gaactgctga
     6181 aacagaatat tgtgagcagt acgctggagc gcctggaaag ttacctgccg gatattaaga
     6241 aacatattga gatcattgaa gttgccaccc caaaaaccct ggaacgctac accctgaaca
     6301 cgaacggtgc cgcgtatggt tgggccgtga gtgttgacca aatctgggaa cagcgtctga
     6361 gcccaacgac gccgattagt ggtctgtatc tggcgggtca ttggacgcgc ccaggtccag
     6421 gtatctgcgc cgtggttagt agtggttgga gtgtggccaa cctgattctg aaaaattggc
     6481 gtgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg
     6541 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     6601 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc
     6661 ctgtagcggc gcattaagcg cggcgggtgt ggtggtt
//
