LOCUS       Exported                6743 bp ds-DNA     circular SYN 16-MAR-2017
DEFINITION  synthetic circular DNA
ACCESSION   N-StrepII-3607
VERSION     .
KEYWORDS    pPJ219
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 16, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..288
                     /note="f1_origin"
     CDS             complement(513..1328)
                     /codon_start=1
                     /note="KanR2"
                     /note="ORF frame 3"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1434..2053
                     /note="pBR322_origin"
     misc_feature    complement(3468..4559)
                     /note="lacI"
     promoter        4937..4955
                     /note="T7_promoter"
     misc_feature    4955..4982
                     /note="lacO"
     misc_feature    5000..5016
                     /note="T7_transl_en_RBS"
     primer_bind     complement(5029..5072)
                     /note="j5_00251_(pet24_n-strep_bb)_reverse"
     misc_feature    5034..5057
                     /note="strep tag"
     primer_bind     5043..5089
                     /note="j5_00252_(linker)_(kuste3607)_forward"
     misc_feature    5058..5063
                     /note="linker"
     gene            5064..6533
                     /note="kuste3607"
     primer_bind     complement(6504..6546)
                     /note="j5_00237_(3607)_reverse"
     primer_bind     6518..6555
                     /note="j5_00248_(pet24_n-strep_bb)_forward"
     misc_feature    6540..6557
                     /note="6xHis"
     terminator      6568..6696
                     /note="T7_terminator"
     misc_feature    complement(6610..6628)
                     /note="T7_Terminal_primer"
     rep_origin      6725..6743
                     /note="f1_origin"
ORIGIN
        1 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
       61 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      121 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
      181 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
      241 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
      301 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
      361 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
      421 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      481 attcaaatat gtatccgctc atgaattaat tcttagaaaa actcatcgag catcaaatga
      541 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
      601 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
      661 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
      721 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta
      781 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
      841 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
      901 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
      961 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     1021 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     1081 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     1141 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     1201 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     1261 aaatcagcat ccatgttgga atttaatcgc ggcctagagc aagacgtttc ccgttgaata
     1321 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgac
     1381 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1441 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1501 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1561 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1621 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1681 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1741 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1801 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1861 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1921 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1981 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2041 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2101 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2161 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2221 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     2281 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     2341 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     2401 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2461 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     2521 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     2581 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     2641 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     2701 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     2761 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     2821 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     2881 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     2941 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     3001 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     3061 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     3121 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
     3181 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
     3241 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
     3301 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
     3361 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
     3421 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt
     3481 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3541 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
     3601 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
     3661 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct
     3721 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta
     3781 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg
     3841 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct
     3901 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga
     3961 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc
     4021 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg
     4081 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct
     4141 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt
     4201 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc
     4261 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc
     4321 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc
     4381 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact
     4441 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga
     4501 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc
     4561 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg
     4621 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag
     4681 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
     4741 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
     4801 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
     4861 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
     4921 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
     4981 cctctagaaa taattttgtt taactttaag aaggagatat acatatggcg tcgtggtctc
     5041 atccgcagtt tgaaaaaggc gccatgagta gcaccaaacc aattaaacca catattcaaa
     5101 gtaccaagat tgacacgctg tatccgagtt acgatgtgat tgttattggt gccggtatcg
     5161 ccggtctgat ttgtgccgcc ttcctggcca aaagtggtaa gaaagccctg ctgattgagc
     5221 aacatttcat tccaggtggc tactgtacca gctttaaacg taagggtttc ctgtttgatg
     5281 cggcggttca tcatattggc ggttgcggta aatggagcgt ggtgggtcgt tgtttcaaaa
     5341 tgctgggcat cgaaatggac ttttaccatc tggacccaat ggataatctg atcttcccaa
     5401 cgtttagcat tgaaattcca gccgatctgg atgaatacct gctgcgcctg caaaagcgtt
     5461 acccacatga aaaggataac attaagtggt tttttcaaga ctttatccgc ctgtaccgcg
     5521 ccaccttcca taatgagaaa agtcaaatca tcgagaaata caaagatatg acgtacggca
     5581 tgatgctgga tcatttcttt gatgatagtg aactgaaaat ggttctgagc ggccagtggg
     5641 gttacgtggg tctggccccg agcaaggcca gcgccattgc catgagccaa atgatgatta
     5701 actatctgaa agatggtgcc ttttttccaa agggtggtac ccaagaattt gccaatgcca
     5761 tttttcgtaa gtttatcgat ttcggcggtc atgttatgct gagtagtaaa gtgaagaaaa
     5821 tcctgaagaa gggcccaagt gttaatggtg tgaaactgaa tgacgataag gaaattcata
     5881 gcagtgttgt ggtgagcaat atcgatgcgc gtcagacctt ttatcatctg ctgaacgacg
     5941 gtaatgatgt ggatgccgtt tacctgaaaa acattgaggg tatgaaagaa agttgtagtt
     6001 tttttctgct gtacctgggt atcggtaaag gcattgacct gaatacgctg aagcgcggct
     6061 tctacctgag tggtagcaat ttcgacagca tcgacagtga atggatgtat attagtgtgc
     6121 caaccagtat ttgtcaagcg ctgagtccac aaaataagca aattatcagt atcgtggtgt
     6181 acaagaaaga agagttctac aaaaatgtga ccgattggga actgctgaaa cagaatattg
     6241 tgagcagtac gctggagcgc ctggaaagtt acctgccgga tattaagaaa catattgaga
     6301 tcattgaagt tgccacccca aaaaccctgg aacgctacac cctgaacacg aacggtgccg
     6361 cgtatggttg ggccgtgagt gttgaccaaa tctgggaaca gcgtctgagc ccaacgacgc
     6421 cgattagtgg tctgtatctg gcgggtcatt ggacgcgccc aggtccaggt atctgcgccg
     6481 tggttagtag tggttggagt gtggccaacc tgattctgaa aaattggcgt taactcgagc
     6541 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     6601 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     6661 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
     6721 cggcgcatta agcgcggcgg gtg
//
