LOCUS       Exported                7918 bp ds-DNA     circular SYN 16-MAR-2017
DEFINITION  synthetic circular DNA
ACCESSION   N-8xHis-StrepII-MBP-3607
VERSION     .
KEYWORDS    pPJ217
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 16, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(1..3)
                     /note="lacO"
     misc_feature    complement(21..37)
                     /note="RBS"
     misc_feature    48..71
                     /note="8xHis"
     misc_feature    81..104
                     /note="StrepII"
     misc_feature    114..1211
                     /note="MBP"
     misc_feature    516..534
                     /note="beast seq1"
     misc_feature    1093..1112
                     /note="MBP seq for"
     misc_feature    1230..1250
                     /note="TEV site"
     primer_bind     complement(1240..1272)
                     /note="j5_00233_(beast_bb)_reverse"
     primer_bind     1247..1285
                     /note="j5_00234_(3607)_forward"
     gene            1260..2729
                     /note="kuste3607"
     misc_feature    1285..1304
                     /note="3607beast seq2"
     misc_feature    2058..2077
                     /note="3607beast seq3"
     primer_bind     complement(2700..2742)
                     /note="j5_00237_(3607)_reverse"
     primer_bind     2717..2751
                     /note="j5_00236_(beast_tag)_forward"
     terminator      complement(2764..2892)
                     /note="T7 terminator"
     misc_feature    complement(2806..2824)
                     /note="T7 term primer"
     CDS             complement(3452..4267)
                     /codon_start=1
                     /note="KanR"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(4373..4992)
                     /direction=LEFT
                     /note="pBR322 ori"
     misc_feature    complement(6407..7498)
                     /note="lacI"
     misc_feature    7876..7895
                     /note="T7 for primer"
     promoter        complement(7876..7894)
                     /note="T7 promoter"
     misc_feature    complement(7894..7918)
                     /note="lacO"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgcat caccaccacc
       61 atcaccacca tggttctgga tggagccatc cgcagtttga aaaaggatca ggtaaaatcg
      121 aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt ctcgctgaag
      181 tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat ccggataaac
      241 tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt atcttctggg
      301 cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc accccggaca
      361 aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac aacggcaagc
      421 tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa gatctgctgc
      481 cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg aaagcgaaag
      541 gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg ctgattgctg
      601 ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa gacgtgggcg
      661 tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt aaaaacaaac
      721 acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa ggcgaaacag
      781 cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa gtgaattatg
      841 gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga
      901 gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc ctcgaaaact
      961 atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag
     1021 cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc actatggaaa
     1081 acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc tggtatgccg
     1141 tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag
     1201 acgcgcagac tgggtctgga tcaggctctg aaaaccttta cttccagggc ggatccggta
     1261 tgagtagcac caaaccaatt aaaccacata ttcaaagtac caagattgac acgctgtatc
     1321 cgagttacga tgtgattgtt attggtgccg gtatcgccgg tctgatttgt gccgccttcc
     1381 tggccaaaag tggtaagaaa gccctgctga ttgagcaaca tttcattcca ggtggctact
     1441 gtaccagctt taaacgtaag ggtttcctgt ttgatgcggc ggttcatcat attggcggtt
     1501 gcggtaaatg gagcgtggtg ggtcgttgtt tcaaaatgct gggcatcgaa atggactttt
     1561 accatctgga cccaatggat aatctgatct tcccaacgtt tagcattgaa attccagccg
     1621 atctggatga atacctgctg cgcctgcaaa agcgttaccc acatgaaaag gataacatta
     1681 agtggttttt tcaagacttt atccgcctgt accgcgccac cttccataat gagaaaagtc
     1741 aaatcatcga gaaatacaaa gatatgacgt acggcatgat gctggatcat ttctttgatg
     1801 atagtgaact gaaaatggtt ctgagcggcc agtggggtta cgtgggtctg gccccgagca
     1861 aggccagcgc cattgccatg agccaaatga tgattaacta tctgaaagat ggtgcctttt
     1921 ttccaaaggg tggtacccaa gaatttgcca atgccatttt tcgtaagttt atcgatttcg
     1981 gcggtcatgt tatgctgagt agtaaagtga agaaaatcct gaagaagggc ccaagtgtta
     2041 atggtgtgaa actgaatgac gataaggaaa ttcatagcag tgttgtggtg agcaatatcg
     2101 atgcgcgtca gaccttttat catctgctga acgacggtaa tgatgtggat gccgtttacc
     2161 tgaaaaacat tgagggtatg aaagaaagtt gtagtttttt tctgctgtac ctgggtatcg
     2221 gtaaaggcat tgacctgaat acgctgaagc gcggcttcta cctgagtggt agcaatttcg
     2281 acagcatcga cagtgaatgg atgtatatta gtgtgccaac cagtatttgt caagcgctga
     2341 gtccacaaaa taagcaaatt atcagtatcg tggtgtacaa gaaagaagag ttctacaaaa
     2401 atgtgaccga ttgggaactg ctgaaacaga atattgtgag cagtacgctg gagcgcctgg
     2461 aaagttacct gccggatatt aagaaacata ttgagatcat tgaagttgcc accccaaaaa
     2521 ccctggaacg ctacaccctg aacacgaacg gtgccgcgta tggttgggcc gtgagtgttg
     2581 accaaatctg ggaacagcgt ctgagcccaa cgacgccgat tagtggtctg tatctggcgg
     2641 gtcattggac gcgcccaggt ccaggtatct gcgccgtggt tagtagtggt tggagtgtgg
     2701 ccaacctgat tctgaaaaat tggcgttaac tcgagcacca ccaccaccac cactgagatc
     2761 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
     2821 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
     2881 ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     2941 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3001 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3061 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     3121 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3181 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3241 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     3301 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc
     3361 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3421 ttcaaatatg tatccgctca tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa
     3481 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     3541 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     3601 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     3661 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat
     3721 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     3781 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     3841 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     3901 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     3961 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     4021 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     4081 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     4141 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     4201 aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat
     4261 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgacc
     4321 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4381 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4441 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4501 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     4561 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4621 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4681 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4741 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4801 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4861 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4921 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4981 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     5041 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     5101 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     5161 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt
     5221 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     5281 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     5341 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     5401 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
     5461 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
     5521 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
     5581 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat
     5641 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
     5701 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
     5761 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag
     5821 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
     5881 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
     5941 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
     6001 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
     6061 ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg
     6121 accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc
     6181 gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg
     6241 cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat
     6301 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
     6361 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
     6421 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6481 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
     6541 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
     6601 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
     6661 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa
     6721 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
     6781 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
     6841 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
     6901 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
     6961 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
     7021 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
     7081 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
     7141 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
     7201 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
     7261 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
     7321 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
     7381 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
     7441 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
     7501 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
     7561 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
     7621 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
     7681 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     7741 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     7801 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc
     7861 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattc
//
