LOCUS       pBzCas13b               8098 bp ds-DNA     circular     09-MAR-2017
DEFINITION  .
KEYWORDS    "accession:addgene_65823_111984"                                   
FEATURES             Location/Qualifiers
     misc_feature    1653..1682
                     /label="Gibson homology EcoRV-pACYC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    5442..5477
                     /label="BZ DR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     RBS             1713..1718
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    5479..5483
                     /label="BsaI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    5500..5535
                     /label="BZ DR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5493..5498
                     /label="BsaI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    5536..5565
                     /label="Gibson homology R EcoRV pACYC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        5402..5436
                     /label="J23119 promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        1683..1712
                     /label="Lac promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1727..5398
                     /label="bzcas13b"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT
       61 GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT
      121 ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA
      181 TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA
      241 AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT
      301 GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC AGGGCTTCCC
      361 GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG TGATCTTCCG TCACAGGTAT
      421 TTATTCGGCG CAAAGTGCGT CGGGTGATGC TGCCAACTTA CTGATTTAGT GTATGATGGT
      481 GTTTTTGAGG TGCTCCAGTG GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG
      541 ACGGGGTGGT GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT
      601 GGCTTACTAT GTTGGCACTG ATGAGGGTGT CAGTGAAGTG CTTCATGTGG CAGGAGAAAA
      661 AAGGCTGCAC CGGTGCGTCA GCAGAATATG TGATACAGGA TATATTCCGC TTCCTCGCTC
      721 ACTGACTCGC TACGCTCGGT CGTTCGACTG CGGCGAGCGG AAATGGCTTA CGAACGGGGC
      781 GGAGATTTCC TGGAAGATGC CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA
      841 AGCCGTTTTT CCATAGGCTC CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC
      901 AGTGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGCGGCTCCC
      961 TCGTGCGCTC TCCTGTTCCT GCCTTTCGGT TTACCGGTGT CATTCCGCTG TTATGGCCGC
     1021 GTTTGTCTCA TTCCACGCCT GACACTCAGT TCCGGGTAGG CAGTTCGCTC CAAGCTGGAC
     1081 TGTATGCACG AACCCCCCGT TCAGTCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     1141 GAGTCCAACC CGGAAAGACA TGCAAAAGCA CCACTGGCAG CAGCCACTGG TAATTGATTT
     1201 AGAGGAGTTA GTCTTGAAGT CATGCGCCGG TTAAGGCTAA ACTGAAAGGA CAAGTTTTGG
     1261 TGACTGCGCT CCTCCAAGCC AGTTACCTCG GTTCAAAGAG TTGGTAGCTC AGAGAACCTT
     1321 CGAAAAACCG CCCTGCAAGG CGGTTTTTTC GTTTTCAGAG CAAGAGATTA CGCGCAGACC
     1381 AAAACGATCT CAAGAAGATC ATCTTATTAA TCAGATAAAA TATTTCTAGA TTTCAGTGCA
     1441 ATTTATCTCT TCAAATGTAG CACCTGAAGT CAGCCCCATA CGATATAAGT TGTAATTCTC
     1501 ATGTTTGACA GCTTATCATC GATAAGCTTT AATGCGGTAG TTTATCACAG TTAAATTGCT
     1561 AACGCAGTCA GGCACCGTGT ATGAAATCTA ACAATGCGCT CATCGTCATC CTCGGCACCG
     1621 TCACCCTGGA TGCTGTAGGC ATAGGCTTGG TTATGCCGGT ACTGCCGGGC CTCTTGCGGG
     1681 ATTTTACACT TTATGCTTCC GGCTCGTATG TTAGGAGGTC TTTATCATGG AAAACAAAAC
     1741 ATCTTTGGGG AATAATATTT ATTACAACCC TTTTAAACCA CAAGATAAAT CGTATTTTGC
     1801 GGGTTATTTC AATGCAGCAA TGGAAAATAC AGATAGTGTA TTTAGAGAAT TGGGAAAAAG
     1861 ATTAAAAGGT AAAGAGTACA CTTCGGAGAA TTTTTTTGAT GCTATCTTTA AGGAAAATAT
     1921 TTCATTAGTG GAATACGAAA GGTATGTGAA ATTGCTTTCT GATTATTTCC CTATGGCTCG
     1981 TCTTTTGGAT AAAAAAGAAG TTCCTATAAA AGAACGAAAA GAAAATTTTA AAAAGAATTT
     2041 TAAAGGCATT ATAAAAGCCG TAAGAGATTT AAGAAACTTT TATACGCATA AGGAACACGG
     2101 AGAGGTAGAA ATTACAGATG AAATATTTGG CGTATTGGAT GAGATGCTGA AAAGCACGGT
     2161 TTTAACGGTA AAAAAGAAAA AAGTAAAAAC CGATAAAACA AAAGAAATCC TGAAAAAAAG
     2221 CATAGAAAAA CAATTGGATA TTTTATGCCA AAAGAAATTA GAATATCTAA GAGACACAGC
     2281 AAGAAAAATA GAGGAGAAGC GTAGAAATCA AAGAGAAAGA GGAGAGAAAG AATTGGTAGC
     2341 TCCATTCAAA TACAGTGATA AAAGGGATGA TTTAATAGCA GCGATTTATA ATGATGCTTT
     2401 TGATGTTTAT ATCGATAAGA AAAAAGATAG CCTAAAAGAA TCCAGCAAAG CAAAGTACAA
     2461 TACCAAAAGT GATCCTCAGC AAGAAGAAGG CGATTTGAAA ATTCCAATTT CGAAAAACGG
     2521 AGTAGTCTTC CTGCTTTCTT TGTTTCTCAC CAAACAAGAA ATACACGCAT TCAAATCTAA
     2581 AATAGCAGGG TTTAAGGCTA CGGTTATCGA TGAAGCAACG GTATCTGAAG CAACGGTGAG
     2641 CCATGGAAAA AACAGCATTT GTTTTATGGC TACGCACGAA ATTTTCTCGC ATTTGGCTTA
     2701 CAAAAAATTG AAACGAAAAG TGAGAACGGC TGAAATCAAC TATGGTGAGG CAGAAAATGC
     2761 TGAACAGCTT TCCGTTTATG CCAAAGAAAC TCTAATGATG CAGATGCTTG ATGAATTGAG
     2821 CAAAGTACCC GATGTGGTCT ATCAGAATTT GAGCGAAGAT GTACAAAAAA CTTTCATCGA
     2881 AGATTGGAAT GAATACCTAA AAGAGAACAA TGGCGATGTA GGGACAATGG AAGAAGAGCA
     2941 GGTAATTCAT CCAGTGATTC GCAAGCGTTA CGAAGATAAA TTCAATTATT TTGCCATTCG
     3001 ATTTTTAGAT GAGTTTGCTC AGTTCCCTAC GCTTCGTTTT CAAGTGCATT TAGGGAATTA
     3061 TCTTCACGAT TCTCGCCCTA AAGAAAATCT GATTTCTGAC CGAAGAATAA AAGAAAAAAT
     3121 CACCGTTTTC GGAAGGCTTT CGGAATTGGA ACATAAAAAA GCATTGTTTA TAAAAAATAC
     3181 CGAAACCAAT GAAGATAGAG AACACTATTG GGAAATTTTT CCTAACCCCA ATTATGATTT
     3241 TCCTAAGGAA AATATTTCCG TTAATGATAA AGATTTTCCT ATCGCGGGTA GTATTTTGGA
     3301 CAGAGAGAAA CAGCCTGTCG CAGGGAAAAT TGGTATAAAA GTGAAATTGC TTAACCAACA
     3361 ATATGTTTCA GAAGTAGATA AAGCCGTAAA AGCCCATCAG CTCAAGCAAA GAAAAGCCAG
     3421 CAAGCCGTCT ATCCAGAACA TTATAGAAGA AATAGTGCCA ATCAATGAAA GCAATCCAAA
     3481 AGAAGCTATT GTTTTCGGTG GGCAACCCAC GGCTTATCTG AGTATGAATG ACATTCATTC
     3541 CATTTTGTAT GAGTTTTTCG ATAAATGGGA GAAAAAGAAA GAAAAGTTAG AAAAGAAAGG
     3601 TGAAAAAGAA TTAAGAAAGG AAATTGGAAA AGAGTTAGAA AAGAAAATCG TAGGAAAAAT
     3661 TCAAGCTCAA ATCCAGCAAA TCATAGATAA AGATACCAAT GCCAAGATAT TAAAACCTTA
     3721 TCAAGACGGA AATTCTACTG CTATTGATAA AGAGAAATTG ATAAAAGATT TGAAGCAAGA
     3781 GCAAAACATT CTACAAAAAT TAAAAGATGA ACAAACGGTT CGTGAAAAAG AATATAATGA
     3841 TTTTATTGCT TATCAAGATA AAAATAGGGA GATAAATAAA GTCAGAGATA GAAATCATAA
     3901 ACAATATTTA AAAGATAACC TTAAACGAAA GTACCCTGAA GCTCCTGCAC GAAAAGAAGT
     3961 TCTTTATTAT CGAGAAAAAG GAAAAGTAGC GGTTTGGTTG GCGAATGACA TCAAACGCTT
     4021 TATGCCTACC GATTTCAAAA ACGAATGGAA AGGGGAGCAA CACAGCTTGT TACAAAAATC
     4081 GTTGGCGTAT TATGAGCAAT GTAAAGAGGA GTTGAAAAAT CTTTTGCCTG AAAAAGTATT
     4141 TCAGCATTTA CCTTTTAAGT TGGGAGGATA TTTTCAACAA AAATATTTGT ACCAATTTTA
     4201 CACTTGTTAT TTAGATAAAC GGTTGGAATA CATCAGTGGG TTGGTGCAAC AAGCCGAAAA
     4261 CTTTAAATCC GAAAATAAAG TCTTCAAAAA GGTAGAAAAT GAGTGTTTTA AATTTTTGAA
     4321 AAAACAAAAT TATACTCACA AAGAATTAGA TGCTCGGGTT CAGTCTATAT TGGGGTATCC
     4381 TATCTTTTTA GAGCGGGGCT TTATGGATGA AAAACCTACG ATAATCAAAG GAAAAACTTT
     4441 TAAAGGAAAT GAAGCTCTTT TTGCCGATTG GTTTAGGTAT TACAAGGAAT ATCAGAATTT
     4501 TCAGACATTT TATGATACTG AAAATTATCC GCTTGTAGAA TTGGAAAAAA AACAAGCAGA
     4561 CCGAAAACGA AAAACTAAAA TCTACCAACA AAAGAAAAAC GATGTGTTTA CACTTTTGAT
     4621 GGCAAAACAT ATTTTCAAAT CGGTATTCAA GCAGGATAGC ATCGATCAGT TTTCGTTAGA
     4681 AGATTTGTAT CAATCTCGGG AGGAACGCTT AGGAAATCAG GAGAGAGCCA GACAAACGGG
     4741 CGAGCGAAAT ACTAACTATA TTTGGAACAA AACCGTTGAT TTAAAGTTGT GTGACGGAAA
     4801 AATTACGGTA GAAAATGTAA AGCTAAAAAA TGTAGGAGAT TTCATAAAAT ATGAATACGA
     4861 CCAAAGGGTT CAAGCTTTCT TAAAATATGA GGAAAATATA GAATGGCAGG CGTTTCTAAT
     4921 CAAAGAGAGT AAGGAAGAGG AAAATTATCC GTATGTGGTG GAAAGAGAAA TAGAGCAATA
     4981 TGAAAAAGTG AGAAGGGAAG AACTGTTGAA GGAAGTCCAC CTTATAGAGG AATATATTTT
     5041 GGAAAAGGTA AAAGATAAGG AAATATTAAA AAAAGGGGAT AATCAAAATT TCAAATACTA
     5101 CATTCTCAAC GGACTTTTAA AGCAACTTAA AAATGAAGAT GTGGAGAGCT ATAAGGTATT
     5161 CAACTTAAAT ACTGAACCTG AAGATGTGAA CATTAACCAA CTAAAACAAG AAGCAACCGA
     5221 TTTAGAACAA AAAGCTTTTG TTTTAACCTA TATTCGTAAT AAATTTGCAC ACAACCAATT
     5281 ACCTAAAAAA GAATTTTGGG ACTATTGTCA AGAAAAATAC GGCAAAATAG AAAAAGAAAA
     5341 AACCTATGCC GAGTATTTTG CTGAGGTATT TAAAAAGGAA AAAGAAGCAC TGATAAAGTA
     5401 ATTGACAGCT AGCTCAGTCC TAGGTATAAT GCTAGCGCTG AGTTGGAACT GCTCTCATTT
     5461 TGGAGGGTAA TCACAACGAG ACCATGGCGG AAGGTCTCAG TTGGAACTGC TCTCATTTTG
     5521 GAGGGTAATC ACAACATCGT CCATTCCGAC AGCATCGCCA GTCACTATGG CGTGCTGCTA
     5581 GCGCTATATG CGTTGATGCA ATTTCTATGC GCACCCGTTC TCGGAGCACT GTCCGACCGC
     5641 TTTGGCCGCC GCCCAGTCCT GCTCGCTTCG CTACTTGGAG CCACTATCGA CTACGCGATC
     5701 ATGGCGACCA CACCCGTCCT GTGGATCCTC TACGCCGGAC GCATCGTGGC CGGCATCACC
     5761 GGCGCCACAG GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG GGAAGATCGG
     5821 GCTCGCCACT TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC AGGCCCCGTG
     5881 GCCGGGGGAC TGTTGGGCGC CATCTCCTTG CATGCACCAT TCCTTGCGGC GGCGGTGCTC
     5941 AACGGCCTCA ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA GGGAGAGCGT
     6001 CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG
     6061 ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG
     6121 GCAGCGCTCT GGGTCATTTT CGGCGAGGAC CGCTTTCGCT GGAGCGCGAC GATGATCGGC
     6181 CTGTCGCTTG CGGTATTCGG AATCTTGCAC GCCCTCGCTC AAGCCTTCGT CACTGGTCCC
     6241 GCCACCAAAC GTTTCGGCGA GAAGCAGGCC ATTATCGCCG GCATGGCGGC CGACGCGCTG
     6301 GGCTACGTCT TGCTGGCGTT CGCGACGCGA GGCTGGATGG CCTTCCCCAT TATGATTCTT
     6361 CTCGCTTCCG GCGGCATCGG GATGCCCGCG TTGCAGGCCA TGCTGTCCAG GCAGGTAGAT
     6421 GACGACCATC AGGGACAGCT TCAAGGATCG CTCGCGGCTC TTACCAGCCT AACTTCGATC
     6481 ATTGGACCGC TGATCGTCAC GGCGATTTAT GCCGCCTCGG CGAGCACATG GAACGGGTTG
     6541 GCATGGATTG TAGGCGCCGC CCTATACCTT GTCTGCCTCC CCGCGTTGCG TCGCGGTGCA
     6601 TGGAGCCGGG CCACCTCGAC CTGAATGGAA GCCGGCGGCA CCTCGCTAAC GGATTCACCA
     6661 CTCCAAGAAT TGGAGCCAAT CAATTCTTGC GGAGAACTGT GAATGCGCAA ACCAACCCTT
     6721 GGCAGAACAT ATCCATCGCG TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA
     6781 GCGTTGGGTC CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG
     6841 GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG CGAACGTGAA
     6901 GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC ATGAATGGTC TTCGGTTTCC
     6961 GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT CCCCTACGTG CTGCTGAAGT TGCCCGCAAC
     7021 AGAGAGTGGA ACCAACCGGT GATACCACGA TACTATGACT GAGAGTCAAC GCCATGAGCG
     7081 GCCTCATTTC TTATTCTGAG TTACAACAGT CCGCACCGCT GTCCGGTAGC TCCTTCCGGT
     7141 GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG
     7201 TAGGACAGGT GCCGGCAGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA
     7261 CGCCGAAACA AGCGCCCTGC ACCATTATGT TCCGGATCTG CATCGCAGGA TGCTGCTGGC
     7321 TACCCTGTGG AACACCTACA TCTGTATTAA CGAAGCGCTA ACCGTTTTTA TCAGGCTCTG
     7381 GGAGGCAGAA TAAATGATCA TATCGTCAAT TATTACCTCC ACGGGGAGAG CCTGAGCAAA
     7441 CTGGCCTCAG GCATTTGAGA AGCACACGGT CACACTGCTT CCGGTAGTCA ATAAACCGGT
     7501 AAACCAGCAA TAGACATAAG CGGCTATTTA ACGACCCTGC CCTGAACCGA CGACCGGGTC
     7561 GAATTTGCTT TCGAATTTCT GCCATTCATC CGCTTATTAT CACTTATTCA GGCGTAGCAC
     7621 CAGGCGTTTA AGGGCACCAA TAACTGCCTT AAAAAAATTA CGCCCCGCCC TGCCACTCAT
     7681 CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA CAGACGGCAT
     7741 GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA TATTTGCCCA
     7801 TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA AAACTGGTGA
     7861 AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT TTAGGGAAAT
     7921 AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA AACTGCCGGA
     7981 AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA TGGAAAACGG
     8041 TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT GCCATACG
//