LOCUS       pBZcsx27                3336 bp ds-DNA     circular     09-MAR-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    961..965
                     /label="RBS"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    866..878
                     /label="CAP protein binding site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    972..974
                     /label="Start codon"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     -35_signal      898..903
                     /label="Plac promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     -10_signal      922..927
                     /label="Plac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             2291..3151
                     /label="bla"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     -10_signal      complement(141..146)
                     /label="RNAI promoter (clockwise); GCTACA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     -35_signal      complement(163..168)
                     /label="RNAI promoter (clockwise); TTGAAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     source          complement(2091..2090)
                     /label="pUC19"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        1025..1054
                     /label="Lac promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     RBS             1055..1060
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     -35_signal      2221..2226
                     /label="bla promoter (counter-clockwise); TTCAAA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_RNA        22..574
                     /label="RNAII transcript (complementary strand)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     -35_signal      3322..3327
                     /label="RNAII promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    935..954
                     /label="lac repressor binding site"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     -10_signal      2242..2247
                     /label="bla promoter (counter-clockwise); GAGACA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(2558..2584)
                     /label="New AGAAW target 2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      3322..574
                     /label="pMB1 origin of replication"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             1069..1674
                     /label="bzcsx27"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_RNA        complement(25..132)
                     /label="RNAI transcript"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     -10_signal      7..12
                     /label="RNAII promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
       61 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
      121 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
      181 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
      241 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
      301 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
      361 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
      421 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
      481 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
      541 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
      601 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      661 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
      721 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
      781 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
      841 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
      901 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      961 aggaaacagc tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg actctagagg
     1021 atccTTTACA CTTTATGCTT CCGGCTCGTA TGTTAGGAGG TCTTTATCat gattgagctg
     1081 ataaattttt cagcggaata tgtttttggg aaagccccac tcattacagc aggaaacctt
     1141 agcgacggtc tgttgttttt tgccattgcc ctatttatag gatgcttgat tcatatcatc
     1201 acattttggt tgataaagag aaagtggtat aagaaattgg cttgtacgcc tatggacaaa
     1261 atgaaaaatg acgagtacat cactgaaatt cttcctgatt tgaaagaaat ttacagaaca
     1321 aataaaggaa tttcggtaac agaaaatatc agtaatggaa gtgtctttga tacagcgtat
     1381 tattatttag aggcacaagg caaaatatcc caagcaaaaa attttcagag tatttatttc
     1441 ttgtttcgga acatcgtaac gctttcgtta tttgtacttc ccgtgagtgt tatcttttta
     1501 ttagcatcct tttttatgaa tgattgttcg ctgagtgaga aaatcattac aattattata
     1561 ggaaccttcg ttctaggtgg attatcatcg gttatagccc aatggtttcg ggtaaaaatg
     1621 acagacagaa ttttcggact ttactatgca gaattaacac acaataaaaa ataaccgggt
     1681 accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     1741 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     1801 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     1861 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     1921 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     1981 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2041 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     2101 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     2161 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2221 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2281 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2341 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2401 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2461 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2521 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2581 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2641 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2701 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2761 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2821 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2881 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2941 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3001 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3061 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3121 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3181 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3241 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3301 agaaaagatc aaaggatctt cttgagatcc tttttt
//