LOCUS       pPbCas13b               7808 bp ds-DNA     circular     09-MAR-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        1683..1712
                     /label="Lac promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5152..5187
                     /label="DR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5210..5245
                     /label="DR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     gene            1727..5110
                     /label="pbcas13b"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             1713..1718
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    5203..5208
                     /label="BsaI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        5112..5146
                     /label="J23119 promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    5189..5193
                     /label="BsaI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT
       61 GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT
      121 ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA
      181 TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA
      241 AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT
      301 GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC AGGGCTTCCC
      361 GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG TGATCTTCCG TCACAGGTAT
      421 TTATTCGGCG CAAAGTGCGT CGGGTGATGC TGCCAACTTA CTGATTTAGT GTATGATGGT
      481 GTTTTTGAGG TGCTCCAGTG GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG
      541 ACGGGGTGGT GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT
      601 GGCTTACTAT GTTGGCACTG ATGAGGGTGT CAGTGAAGTG CTTCATGTGG CAGGAGAAAA
      661 AAGGCTGCAC CGGTGCGTCA GCAGAATATG TGATACAGGA TATATTCCGC TTCCTCGCTC
      721 ACTGACTCGC TACGCTCGGT CGTTCGACTG CGGCGAGCGG AAATGGCTTA CGAACGGGGC
      781 GGAGATTTCC TGGAAGATGC CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA
      841 AGCCGTTTTT CCATAGGCTC CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC
      901 AGTGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGCGGCTCCC
      961 TCGTGCGCTC TCCTGTTCCT GCCTTTCGGT TTACCGGTGT CATTCCGCTG TTATGGCCGC
     1021 GTTTGTCTCA TTCCACGCCT GACACTCAGT TCCGGGTAGG CAGTTCGCTC CAAGCTGGAC
     1081 TGTATGCACG AACCCCCCGT TCAGTCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     1141 GAGTCCAACC CGGAAAGACA TGCAAAAGCA CCACTGGCAG CAGCCACTGG TAATTGATTT
     1201 AGAGGAGTTA GTCTTGAAGT CATGCGCCGG TTAAGGCTAA ACTGAAAGGA CAAGTTTTGG
     1261 TGACTGCGCT CCTCCAAGCC AGTTACCTCG GTTCAAAGAG TTGGTAGCTC AGAGAACCTT
     1321 CGAAAAACCG CCCTGCAAGG CGGTTTTTTC GTTTTCAGAG CAAGAGATTA CGCGCAGACC
     1381 AAAACGATCT CAAGAAGATC ATCTTATTAA TCAGATAAAA TATTTCTAGA TTTCAGTGCA
     1441 ATTTATCTCT TCAAATGTAG CACCTGAAGT CAGCCCCATA CGATATAAGT TGTAATTCTC
     1501 ATGTTTGACA GCTTATCATC GATAAGCTTT AATGCGGTAG TTTATCACAG TTAAATTGCT
     1561 AACGCAGTCA GGCACCGTGT ATGAAATCTA ACAATGCGCT CATCGTCATC CTCGGCACCG
     1621 TCACCCTGGA TGCTGTAGGC ATAGGCTTGG TTATGCCGGT ACTGCCGGGC CTCTTGCGGG
     1681 ATTTTACACT TTATGCTTCC GGCTCGTATG TTAGGAGGTC TTTATCATGC AAAAACAAGA
     1741 TAAATTATTT GTAGATAGAA AGAAAAATGC TATATTTGCC TTTCCTAAAT ATATAACAAT
     1801 AATGGAAAAC AAAGAGAAAC CTGAACCAAT TTATTATGAA TTGACAGATA AGCATTTCTG
     1861 GGCTGCCTTT CTTAACTTGG CGCGCCACAA TGTCTATACC ACCATTAATC ACATCAACAG
     1921 ACGTTTGGAA ATAGCAGAGC TAAAAGACGA TGGATATATG ATGGGCATTA AAGGCAGCTG
     1981 GAACGAGCAA GCGAAAAAAC TCGACAAAAA AGTTCGGCTG AGAGACTTGA TCATGAAACA
     2041 CTTTCCTTTT CTGGAAGCTG CAGCTTATGA AATGACAAAC TCTAAAAGTC CCAACAATAA
     2101 GGAGCAACGG GAAAAGGAAC AATCCGAAGC CCTATCACTT AACAACTTGA AGAATGTTCT
     2161 ATTCATTTTC TTGGAAAAAC TTCAGGTTCT CAGGAACTAC TACTCCCATT ACAAGTATTC
     2221 TGAAGAATCC CCAAAACCGA TATTTGAAAC AAGCCTTTTG AAAAACATGT ATAAGGTTTT
     2281 CGATGCCAAT GTGCGGTTAG TAAAGAGAGA CTATATGCAT CATGAGAATA TAGACATGCA
     2341 AAGAGATTTT ACACATCTCA ACAGGAAAAA ACAAGTGGGA CGAACAAAAA ACATCATAGA
     2401 CAGTCCTAAC TTTCATTACC ATTTTGCAGA TAAAGAAGGC AATATGACCA TAGCAGGCTT
     2461 ACTGTTCTTC GTCTCTTTAT TTCTTGATAA AAAAGACGCT ATTTGGATGC AGAAAAAGCT
     2521 AAAGGGATTT AAAGATGGCA GAAACTTGCG TGAGCAAATG ACAAACGAAG TATTTTGCAG
     2581 AAGCCGTATA TCTTTGCCTA AGCTAAAATT AGAGAATGTA CAGACAAAAG ACTGGATGCA
     2641 ACTTGATATG CTTAACGAAT TGGTTCGCTG TCCCAAATCG CTGTATGAAC GGTTACGAGA
     2701 AAAAGATAGG GAAAGTTTTA AAGTACCTTT CGATATTTTC TCTGACGACT ATAATGCTGA
     2761 AGAAGAACCA TTTAAGAACA CGCTTGTAAG GCATCAAGAC CGCTTTCCAT ATTTTGTCTT
     2821 GAGATATTTC GATTTAAATG AGATATTCGA ACAACTCCGC TTCCAAATAG ATTTAGGTAC
     2881 CTATCATTTC TCTATCTATA ACAAACGTAT CGGTGACGAA GATGAAGTTC GCCATCTCAC
     2941 ACATCATCTA TATGGCTTTG CGCGCATTCA GGACTTTGCA CCACAAAACC AACCAGAAGA
     3001 ATGGAGAAAA TTAGTGAAAG ACTTAGATCA TTTTGAGACA TCGCAAGAGC CTTACATATC
     3061 TAAGACTGCT CCACATTACC ATTTAGAGAA TGAAAAGATA GGCATTAAGT TTTGCAGTGC
     3121 CCACAACAAC CTCTTTCCAT CTCTTCAGAC CGACAAAACA TGCAATGGAA GAAGTAAATT
     3181 TAATCTCGGA ACACAATTTA CAGCAGAAGC ATTTTTAAGT GTCCACGAAT TGTTGCCGAT
     3241 GATGTTTTAT TATCTGTTAT TGACCAAGGA TTACTCCAGA AAAGAAAGCG CAGACAAAGT
     3301 AGAGGGAATT ATAAGAAAAG AAATAAGCAA CATATACGCT ATCTACGATG CTTTTGCCAA
     3361 TAATGAGATA AATAGCATTG CAGACTTAAC CCGTCGGCTT CAAAATACAA ATATACTACA
     3421 AGGCCATTTG CCAAAACAAA TGATTTCCAT ATTGAAAGGC AGACAAAAAG ATATGGGAAA
     3481 AGAGGCTGAG CGCAAAATTG GGGAAATGAT AGACGACACC CAAAGAAGAC TTGACTTGCT
     3541 CTGCAAGCAG ACAAATCAGA AGATAAGGAT TGGCAAAAGG AATGCCGGTC TGCTAAAATC
     3601 GGGAAAAATT GCAGATTGGT TGGTAAATGA CATGATGCGC TTCCAACCTG TTCAAAAAGA
     3661 CCAAAATAAC ATTCCAATAA ACAATAGCAA GGCTAACAGC ACCGAATATC GAATGCTTCA
     3721 GCGTGCGCTT GCCCTTTTCG GCAGTGAGAA CTTCCGCCTT AAGGCATATT TTAATCAAAT
     3781 GAATCTTGTG GGGAATGACA ATCCGCATCC TTTCCTTGCA GAAACACAAT GGGAACATCA
     3841 AACTAACATT CTGTCTTTCT ACCGAAACTA TTTGGAAGCG AGGAAAAAGT ATCTTAAGGG
     3901 CTTAAAACCA CAAAACTGGA AACAATATCA GCATTTTCTC ATACTTAAAG TACAGAAGAC
     3961 CAACCGCAAC ACATTAGTTA CAGGATGGAA AAACAGTTTC AATCTCCCCC GTGGAATATT
     4021 TACCCAACCA ATAAGGGAAT GGTTTGAGAA ACATAATAAT TCAAAAAGAA TATACGACCA
     4081 AATACTATCT TTTGACCGAG TAGGGTTTGT TGCAAAAGCT ATTCCCTTAT ACTTTGCAGA
     4141 AGAATATAAA GACAATGTAC AGCCTTTCTA CGATTATCCG TTCAATATCG GCAACAGACT
     4201 TAAACCAAAA AAAAGGCAAT TCTTAGATAA AAAAGAGCGT GTCGAATTGT GGCAAAAGAA
     4261 TAAAGAGCTA TTCAAAAATT ATCCGTCTGA AAAGAAAAAG ACAGACTTAG CTTATCTTGA
     4321 TTTTTTATCA TGGAAAAAGT TCGAACGCGA ACTGCGACTT ATCAAGAATC AGGATATCGT
     4381 CACATGGTTG ATGTTCAAAG AACTATTCAA TATGGCTACT GTGGAAGGAT TGAAGATTGG
     4441 AGAAATACAT CTGAGGGACA TCGATACAAA CACGGCAAAT GAGGAAAGCA ACAATATTCT
     4501 AAATAGAATC ATGCCCATGA AGTTGCCTGT TAAAACATAC GAAACAGACA ATAAAGGAAA
     4561 TATATTAAAG GAAAGACCTT TAGCAACCTT TTATATCGAA GAAACGGAAA CCAAAGTCCT
     4621 GAAACAGGGC AATTTCAAAG CATTGGTAAA AGACCGAAGA CTCAATGGGC TATTCTCATT
     4681 TGCAGAAACT ACAGACTTGA ACTTAGAGGA ACATCCTATC AGCAAATTGA GTGTAGACCT
     4741 TGAGCTTATA AAATATCAGA CTACAAGGAT TAGTATCTTT GAGATGACAT TAGGACTGGA
     4801 AAAGAAACTC ATAGACAAAT ATTCCACACT ACCTACAGAT AGCTTCAGAA ATATGCTTGA
     4861 AAGGTGGTTG CAGTGCAAAG CTAATAGACC GGAATTAAAG AATTACGTTA ATAGTTTGAT
     4921 TGCAGTGAGA AATGCTTTTT CCCACAACCA GTATCCCATG TATGATGCCA CACTCTTTGC
     4981 TGAAGTTAAA AAATTTACTC TTTTCCCTTC TGTCGATACA AAAAAGATTG AGCTAAATAT
     5041 AGCGCCACAG TTACTGGAAA TAGTAGGAAA AGCCATAAAA GAAATTGAAA AATCAGAAAA
     5101 TAAAAACTAA ATTGACAGCT AGCTCAGTCC TAGGTATAAT GCTAGCGCTG AGTTGCATCT
     5161 GCCTTCTTTT TGAAAGGTAA AAACAACGAG ACCATGGCGG AAGGTCTCAG TTGCATCTGC
     5221 CTTCTTTTTG AAAGGTAAAA ACAACATCGT CCATTCCGAC AGCATCGCCA GTCACTATGG
     5281 CGTGCTGCTA GCGCTATATG CGTTGATGCA ATTTCTATGC GCACCCGTTC TCGGAGCACT
     5341 GTCCGACCGC TTTGGCCGCC GCCCAGTCCT GCTCGCTTCG CTACTTGGAG CCACTATCGA
     5401 CTACGCGATC ATGGCGACCA CACCCGTCCT GTGGATCCTC TACGCCGGAC GCATCGTGGC
     5461 CGGCATCACC GGCGCCACAG GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG
     5521 GGAAGATCGG GCTCGCCACT TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC
     5581 AGGCCCCGTG GCCGGGGGAC TGTTGGGCGC CATCTCCTTG CATGCACCAT TCCTTGCGGC
     5641 GGCGGTGCTC AACGGCCTCA ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA
     5701 GGGAGAGCGT CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC
     5761 GCGGGGCATG ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG
     5821 ACAGGTGCCG GCAGCGCTCT GGGTCATTTT CGGCGAGGAC CGCTTTCGCT GGAGCGCGAC
     5881 GATGATCGGC CTGTCGCTTG CGGTATTCGG AATCTTGCAC GCCCTCGCTC AAGCCTTCGT
     5941 CACTGGTCCC GCCACCAAAC GTTTCGGCGA GAAGCAGGCC ATTATCGCCG GCATGGCGGC
     6001 CGACGCGCTG GGCTACGTCT TGCTGGCGTT CGCGACGCGA GGCTGGATGG CCTTCCCCAT
     6061 TATGATTCTT CTCGCTTCCG GCGGCATCGG GATGCCCGCG TTGCAGGCCA TGCTGTCCAG
     6121 GCAGGTAGAT GACGACCATC AGGGACAGCT TCAAGGATCG CTCGCGGCTC TTACCAGCCT
     6181 AACTTCGATC ATTGGACCGC TGATCGTCAC GGCGATTTAT GCCGCCTCGG CGAGCACATG
     6241 GAACGGGTTG GCATGGATTG TAGGCGCCGC CCTATACCTT GTCTGCCTCC CCGCGTTGCG
     6301 TCGCGGTGCA TGGAGCCGGG CCACCTCGAC CTGAATGGAA GCCGGCGGCA CCTCGCTAAC
     6361 GGATTCACCA CTCCAAGAAT TGGAGCCAAT CAATTCTTGC GGAGAACTGT GAATGCGCAA
     6421 ACCAACCCTT GGCAGAACAT ATCCATCGCG TCCGCCATCT CCAGCAGCCG CACGCGGCGC
     6481 ATCTCGGGCA GCGTTGGGTC CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG
     6541 ACCCGGCTAG GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG
     6601 CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC ATGAATGGTC
     6661 TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT CCCCTACGTG CTGCTGAAGT
     6721 TGCCCGCAAC AGAGAGTGGA ACCAACCGGT GATACCACGA TACTATGACT GAGAGTCAAC
     6781 GCCATGAGCG GCCTCATTTC TTATTCTGAG TTACAACAGT CCGCACCGCT GTCCGGTAGC
     6841 TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC
     6901 ATGCAACTCG TAGGACAGGT GCCGGCAGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC
     6961 ACCATACCCA CGCCGAAACA AGCGCCCTGC ACCATTATGT TCCGGATCTG CATCGCAGGA
     7021 TGCTGCTGGC TACCCTGTGG AACACCTACA TCTGTATTAA CGAAGCGCTA ACCGTTTTTA
     7081 TCAGGCTCTG GGAGGCAGAA TAAATGATCA TATCGTCAAT TATTACCTCC ACGGGGAGAG
     7141 CCTGAGCAAA CTGGCCTCAG GCATTTGAGA AGCACACGGT CACACTGCTT CCGGTAGTCA
     7201 ATAAACCGGT AAACCAGCAA TAGACATAAG CGGCTATTTA ACGACCCTGC CCTGAACCGA
     7261 CGACCGGGTC GAATTTGCTT TCGAATTTCT GCCATTCATC CGCTTATTAT CACTTATTCA
     7321 GGCGTAGCAC CAGGCGTTTA AGGGCACCAA TAACTGCCTT AAAAAAATTA CGCCCCGCCC
     7381 TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA
     7441 CAGACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA
     7501 TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA
     7561 AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT
     7621 TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA
     7681 AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA
     7741 TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT
     7801 GCCATACG
//