LOCUS       BB1_L_23_syn_BsaI_1      1966 bp    DNA     circular UNA 07-Jan-2015
DEFINITION  old name: BB1_linker_FS2_BsaI_FS3
FEATURES             Location/Qualifiers
     primer          complement(17..33)
                     /label=Seq_BB1_BW
     CDS             216..1034
                     /label=KanR
     oriT            1080..1695
                     /label="ColE1 origin, high copy number"
     primer          1722..1739
                     /label=Seq_BB1_FW
     Site            1849..1915
                     /site_type="cleavage site"
                     /label="Linker FS2 FS3"
     FS              1865..1868
                     /label=FS2
     FS              1897..1900
                     /label=FS3
ORIGIN
        1 ATCTAGTCAG CTTGATGTCA TAGCTGTTTC CTGAGGCTCA ATACTGACCA TTTAAATCAT
       61 ACCTGACCTC CATAGCAGAA AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGATCGGC
      121 ACGTAAGAGG TTCCAACTTT CACCATAATG AAATAAGATC ACTACCGGGC GTATTTTTTG
      181 AGTTATCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATGAG CCATATTCAA CGGGAAACGT
      241 CTTGCTTGAA GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG
      301 CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG ATTGTATGGG AAGCCCGATG
      361 CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA
      421 TGGTCAGGCT AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC
      481 GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC AGGGAAAACA GCATTCCAGG
      541 TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC
      601 GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA CGGCGATCGC GTATTTCGTC
      661 TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGG TGCGAGTGAT TTTGATGACG
      721 AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTC TTGCCATTCT
      781 CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG
      841 GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC
      901 TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC
      961 AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCACTTG ATGCTCGATG
     1021 AGTTTTTCTA ATGAGGACCT AAATGTAATC ACCTGGCTCA CCTTCGGGTG GGCCTTTCTG
     1081 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGATGCT
     1141 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA
     1201 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC
     1261 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT
     1321 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG
     1381 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG
     1441 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT
     1501 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC
     1561 TGAAGCCAGT TACCTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     1621 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     1681 AGAAGATCCT TTGATTTTCT ACCGAAGAAA GGCCCACCCG TGTAAAACGA CGGCCAGTTT
     1741 ATCTAGTCAG CTTGATTCTA GCTGATCGTG GACCGGAAGG TGAGCCAGTG AGTTGATTGC
     1801 AGTCCAGTTA CGCTGGAGTC TGAGGCTCGT CCTGAATGAT ATGCGGCCTC GAATTCGAAG
     1861 ACGCCATGTG AGACCGGATC CATCTCGAGG GTCTCAGCTT GCGTCTTCGA ATTCGCGCGT
     1921 GATCTTACGG CATTATACGT ATGATCGGTC CACGATCAGC TAGATT
//
