LOCUS       BB1_L_34_syn_BsaI_3      1966 bp    DNA     circular UNA 07-Jan-2015
DEFINITION  
FEATURES             Location/Qualifiers
     primer          complement(41..57)
                     /label=Seq_BB1_BW
     CDS             240..1058
                     /label=KanR
     oriT            1104..1719
                     /label="ColE1 origin, high copy number"
     primer          1746..1763
                     /label=Seq_BB1_FW
     Site            1873..1939
                     /site_type="cleavage site"
                     /label="Linker FS3 FS4"
     FS              1889..1892
                     /label=FS3
     FS              1921..1924
                     /label=FS4
ORIGIN
        1 GATCGGTCCA CGATCAGCTA GATTATCTAG TCAGCTTGAT GTCATAGCTG TTTCCTGAGG
       61 CTCAATACTG ACCATTTAAA TCATACCTGA CCTCCATAGC AGAAAGTCAA AAGCCTCCGA
      121 CCGGAGGCTT TTGACTTGAT CGGCACGTAA GAGGTTCCAA CTTTCACCAT AATGAAATAA
      181 GATCACTACC GGGCGTATTT TTTGAGTTAT CGAGATTTTC AGGAGCTAAG GAAGCTAAAA
      241 TGAGCCATAT TCAACGGGAA ACGTCTTGCT TGAAGCCGCG ATTAAATTCC AACATGGATG
      301 CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT
      361 ATCGATTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG
      421 TTGCCAATGA TGTTACAGAT GAGATGGTCA GGCTAAACTG GCTGACGGAA TTTATGCCTC
      481 TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA
      541 TCCCAGGGAA AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG
      601 TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT
      661 TTAACGGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG
      721 TTGGTGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG
      781 AAATGCATAA ACTCTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC
      841 TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG
      901 GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC
      961 CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT
     1021 TGCAGTTTCA CTTGATGCTC GATGAGTTTT TCTAATGAGG ACCTAAATGT AATCACCTGG
     1081 CTCACCTTCG GGTGGGCCTT TCTGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
     1141 ACGAGCATCA CAAAAATCGA TGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
     1201 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
     1261 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
     1321 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
     1381 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
     1441 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
     1501 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA
     1561 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTC GGAAAAAGAG TTGGTAGCTC
     1621 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     1681 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATT TTCTACCGAA GAAAGGCCCA
     1741 CCCGTGTAAA ACGACGGCCA GTTTATCTAG TCAGCTTGAT TCTAGCTGAT CGTGGACCGG
     1801 AAGGTGAGCC AGTGAGTTGA TTGCAGTCCA GTTACGCTGG AGTCTGAGGC TCGTCCTGAA
     1861 TGATATGCGG CCTCGAATTC GAAGACGCGC TTTGAGACCG GATCCATCTC GAGGGTCTCA
     1921 CGCTGCGTCT TCGAATTCGC GCGTGATCTT ACGGCATTAT ACGTAT
//
