LOCUS       BB2_L_AB_syn_BbsI_6      2215 bp    DNA     circular UNA 
COMMENT     Note: the plasmid backbone was PCR amplified, only the linker sequence was checked by sequencing! (compare Seq folder)
FEATURES             Location/Qualifiers
     Vector          join(285..2215,1..184)
                     /origin="PCRproductNewBB2 (2.125bp circular)Target
                     vector: from AgeI cut at 5^6 to SpeI cut at 2116^2117,
                     2.111bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     primer_bind     59..76
                     /label=pSTBlue_Seq_FW
     primer_bind     128..145
                     /label=BB2_seq_fw
     primer_bind     complement(168..184)
                     /label=BB2_BW
     FS              238..241
                     /label="FS A"
     FS              242..245
                     /label="FS 1"
     FS              274..277
                     /label="FS 4"
     FS              278..281
                     /label="FS B"
     primer          complement(285..288)
                     /label=6_BsaI_FsB
     primer_bind     295..310
                     /label=BB2_FW
     Region          complement(<295..311)
                     /label="f1 origin"
     primer_bind     complement(386..405)
                     /label=BB2_seq_bw
     CDS             443..1300
                     /label=AmpicillinR
     misc_feature    1151..1167
                     /feature_type="Primer Region"
                     /label=BB2_BsaIMut_FW
     misc_feature    complement(1151..1167)
                     /feature_type="Primer Region"
                     /label=BB2_BsaIMut_BW
     primer          1159
                     /label="G changed to C"
     Region          2116..2173
                     /label="pUC origin"
ORIGIN
        1 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
       61 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG
      121 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
      181 CAACACTAGT CGCAAAAAAC CCCGCCCCTG ACAGGGCGGG GTTTTTTCGC GGTCTCCGAT
      241 CGGAGGCGTC TTCGGATCCC TCGAGGAAGA CGCCGCTCCG GGGAGACCAC CGGTGCCGCT
      301 ACAGGGCGCG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT
      361 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA
      421 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT
      481 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA
      541 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA
      601 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT
      661 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT
      721 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA
      781 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC
      841 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT
      901 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA
      961 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC
     1021 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA
     1081 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC
     1141 TGGAGCCGGT GAGCGTGGCT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC
     1201 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG
     1261 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA
     1321 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA
     1381 GATCCTTTTT GATAATCTCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA
     1441 GGGGTGTAGA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG
     1501 ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT
     1561 GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC
     1621 CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC
     1681 TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG
     1741 CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT
     1801 TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT
     1861 GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC
     1921 TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA
     1981 GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA
     2041 GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG
     2101 GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT
     2161 GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCG
//
