LOCUS       BB2_L_EF_syn_BbsI_10_corrected_sequence_from_addgene      2215 bp    DNA     circular UNA 
COMMENT     Note: the plasmid backbone was PCR amplified, only the linker sequence was checked by sequencing! (compare Seq folder)
FEATURES             Location/Qualifiers
     Vector          join(244..2215,1..139)
                     /origin="PCRproductNewBB2 (2.125bp circular)Target
                     vector: from AgeI cut at 5^6 to SpeI cut at 2116^2117,
                     2.111bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     primer_bind     13..30
                     /label=pSTBlue_Seq_FW
     primer_bind     82..99
                     /label=BB2_seq_fw
     primer_bind     complement(122..138)
                     /label=BB2_BW
     primer          140..184
                     /label=1_B1007_TT_BsaI
     primer          complement(144..191)
                     /label=2_B1007_TT_BsaI
     primer          185..214
                     /label=3_BsaI_FsD_Bam
     Region          185..190
                     /label=BsaI
     primer          complement(192..221)
                     /label=4_BsaI_FsD_BamXho
     FS              192..195
                     /label="FS E"
     FS              196..199
                     /label="FS 1"
     primer          215..243
                     /label=5_BsaI_FsE_Xho
     primer          complement(222..247)
                     /label=6_BsaI_FsE
     FS              228..231
                     /label="FS 4"
     FS              232..235
                     /label="FS F"
     Region          complement(237..242)
                     /label=BsaI
     primer_bind     249..264
                     /label=BB2_FW
     Region          complement(<249..265)
                     /label="f1 origin"
     primer_bind     complement(340..359)
                     /label=BB2_seq_bw
     CDS             397..1254
                     /label=AmpicillinR
     misc_feature    1105..1121
                     /feature_type="Primer Region"
                     /label=BB2_BsaIMut_FW
     misc_feature    complement(1105..1121)
                     /feature_type="Primer Region"
                     /label=BB2_BsaIMut_BW
     primer          1113
                     /label="G changed to C"
     Region          2070..2127
                     /label="pUC origin"
ORIGIN
        1 ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT
       61 CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA
      121 GCGGGCAGTG AGCGCAACAC TAGTcgcaaa aaaccccgcc cctgacaggg cggggttttt
      181 tcgcGGTCTC CAAGCAGCGG CGTCTTCCTC GAGGGATCCG AAGACGCCTC CCTCCGGAGA
      241 CCACCGGTGC CGCTACAGGG CGCGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC
      301 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC
      361 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC
      421 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG
      481 GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT
      541 CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC
      601 ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA
      661 CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA
      721 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT
      781 GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT
      841 TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT
      901 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG
      961 CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG
     1021 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT
     1081 ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGCTCTCGCG GTATCATTGC AGCACTGGGG
     1141 CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG
     1201 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG
     1261 TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA
     1321 AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGAACA ATAAAACTGT CTGCTTACAT
     1381 AAACAGTAAT ACAAGGGGTG TAGATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT
     1441 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT
     1501 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC
     1561 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC
     1621 AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC
     1681 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC
     1741 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG
     1801 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA
     1861 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA
     1921 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC
     1981 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG
     2041 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT
     2101 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT
     2161 GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACG
//
