LOCUS       BB2_L_HI_syn_BbsI_13      2214 bp    DNA     circular UNA 
COMMENT     Note: the plasmid backbone was PCR amplified, only the linker sequence was checked by sequencing! (compare Seq folder)
FEATURES             Location/Qualifiers
     Vector          join(268..2214,1..164)
                     /origin="PCRproductNewBB2 (2.125bp circular)Target
                     vector: from AgeI cut at 5^6 to SpeI cut at 2116^2117,
                     2.111bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     primer_bind     38..55
                     /label=pSTBlue_Seq_FW
     primer_bind     107..124
                     /label=BB2_seq_fw
     primer_bind     complement(147..163)
                     /label=BB2_BW
     primer          165..209
                     /label=1_B1007_TT_BsaI
     primer          complement(169..216)
                     /label=2_B1007_TT_BsaI
     primer          210..239
                     /label=3_BsaI_FsF_Bam
     Region          210..215
                     /label=BsaI
     primer          complement(217..246)
                     /label=4_BsaI_FsF_BamXho
     FS              217..220
                     /label="FS H"
     FS              221..224
                     /label="FS 1"
     primer          240..267
                     /label=5_BsaI_FsG_Xho
     primer          complement(247..271)
                     /label=6_BsaI_FsG
     FS              253..256
                     /label="FS 4"
     FS              256..259
                     /label="FS I"
     Region          complement(261..266)
                     /label=BsaI
     primer_bind     273..288
                     /label=BB2_FW
     Region          complement(<273..289)
                     /label="f1 origin"
     primer_bind     complement(364..383)
                     /label=BB2_seq_bw
     CDS             421..1278
                     /label=AmpicillinR
     misc_feature    1129..1145
                     /feature_type="Primer Region"
                     /label=BB2_BsaIMut_FW
     misc_feature    complement(1129..1145)
                     /feature_type="Primer Region"
                     /label=BB2_BsaIMut_BW
     primer          1137
                     /label="G changed to C"
     Region          2094..2151
                     /label="pUC origin"
ORIGIN
        1 TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA
       61 AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG
      121 GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACACTAGTc gcaaaaaacc
      181 ccgcccctga cagggcgggg ttttttcgcG GTCTCCCAAC GGAGGCGTCT TCGGATCCCT
      241 CGAGGAAGAC GCCGCTGGTG GAGACCACCG GTGCCGCTAC AGGGCGCGTC AGGTGGCACT
      301 TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG
      361 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT
      421 ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT
      481 GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA
      541 CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC
      601 GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC
      661 CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG
      721 GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA
      781 TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC
      841 GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT
      901 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG
      961 CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT
     1021 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC
     1081 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGCTCT
     1141 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC
     1201 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC
     1261 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT
     1321 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG
     1381 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTAGATC TCATGACCAA
     1441 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG
     1501 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC
     1561 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC
     1621 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA
     1681 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT
     1741 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC
     1801 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG
     1861 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC
     1921 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC
     1981 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT
     2041 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC
     2101 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT
     2161 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGC
//
