LOCUS       pCMV_dCas9_MC_eG        8873 bp ds-DNA     circular     22-MAR-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        5640..6082
                     /vntifkey="29"
                     /label=SFFV
     misc_feature    918..5018
                     /label=dCas9
     misc_feature    5019..5126
                     /label=LFL
     rep_origin      8188..8870
                     /label=ColE1 origin
     misc_feature    5475..5495
                     /label=SV40 NLS
     misc_feature    7233..8093
                     /label=AmpR
     CDS             6148..6863
                     /vntifkey="4"
                     /label=eGFP
     misc_feature    888..911
                     /label=FLAG tag
     misc_feature    828..887
                     /label=Nucleoplasmin NLS
     promoter        177..793
                     /label=CMV promoter
     misc_feature    5127..5468
                     /label=M.SssI [273-386]
ORIGIN
        1 ttacggttcc tggccttttg ctggcctttt gctcacatgt ctggtgaatt caagtcgagg
       61 tcgacggtat cgataagctc gcttcacgag attccagcag gtcgagggac ctaataactt
      121 cgtatagcat acattatacg aagttatatt aagggttcca agcttaagtC GCGTTGACAT
      181 TGATTATTGA CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT
      241 ATGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC
      301 CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC
      361 CATTGACGTC AATGGGTGGA gTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG
      421 TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT
      481 TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC
      541 ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT
      601 GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC
      661 CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC
      721 GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC
      781 ACTGCTTACT GGCAtcccag tgtggtggta cgggaattGA CGTCggcatg CCAgtgaaga
      841 gaccTgctgc caccaagaaa gctggccagg ccaaaaagaa gaaactgGAC TACAAAGACG
      901 ATGACGACAA GACGCGTGAT AAGAAATACA GCATAGGACT GgccATTGGA ACAAATTCTG
      961 TCGGCTGGGC GGTTATTACC GATGAATATA AGGTTCCGTC CAAGAAATTC AAGGTTCTAG
     1021 GCAATACAGA CCGCCACAGT ATTAAAAAGA ATTTGATAGG TGCCCTTCTG TTTGACAGTG
     1081 GCGAGACAGC GGAAGCGACC AGACTCAAAC GAACAGCCAG GAGACGTTAT ACAAGACGAA
     1141 AGAATAGGAT CTGTTATCTT CAAGAAATCT TTAGCAATGA GATGGCGAAA GTGGATGATA
     1201 GTTTCTTTCA TCGCCTTGAA GAGTCCTTTC TGGTAGAAGA AGACAAGAAG CATGAAAGAC
     1261 ATCCCATCTT TGGCAATATA GTGGATGAAG TTGCCTATCA TGAGAAATAT CCAACCATCT
     1321 ACCATCTACG CAAGAAACTG GTGGATTCCA CCGATAAAGC GGATCTGCGC CTGATCTATC
     1381 TAGCACTAGC GCACATGATC AAGTTTAGAG GGCATTTTCT GATCGAGGGC GATCTGAATC
     1441 CCGATAATAG TGATGTAGAC AAACTTTTCA TTCAACTGGT GCAGACTTAC AATCAGCTGT
     1501 TTGAAGAAAA CCCCATCAAC GCTAGTGGCG TGGATGCCAA AGCGATCCTT TCCGCTCGCC
     1561 TGAGTAAAAG CAGACGCCTG GAAAATCTCA TCGCCCAACT CCCGGGGGAG AAGAAAAATG
     1621 GACTGTTTGG TAACCTCATC GCCCTGAGCC TGGGGCTGAC TCCCAATTTT AAAAGCAATT
     1681 TTGATCTGGC TGAAGATGCC AAACTGCAAC TTAGCAAAGA TACCTACGAT GATGATCTGG
     1741 ATAATCTGCT GGCGCAGATC GGCGATCAGT ATGCCGATCT GTTTCTGGCT GCCAAGAATC
     1801 TGAGCGATGC CATCCTGCTT AGCGATATTT TGAGAGTGAA TACCGAAATA ACCAAGGCCC
     1861 CGTTGAGCGC CAGCATGATC AAACGCTACG ATGAACATCA TCAGGACCTG ACCCTTCTGA
     1921 AAGCCCTGGT TCGCCAGCAG CTTCCTGAAA AGTATAAAGA GATTTTCTTT GATCAGAGCA
     1981 AGAACGGCTA TGCTGGGTAT ATCGATGGTG GCGCCTCTCA GGAAGAGTTC TATAAGTTCA
     2041 TTAAACCAAT CCTGGAAAAG ATGGATGGGA CCGAGGAACT GCTGGTAAAA TTGAATAGGG
     2101 AGGATCTGCT ACGCAAGCAG CGAACTTTTG ACAACGGGTC CATCCCGCAT CAGATCCACC
     2161 TGGGGGAGCT ACATGCCATC CTGAGAAGAC AGGAAGACTT TTATCCATTT CTGAAAGACA
     2221 ATCGGGAGAA GATCGAAAAG ATTCTGACCT TTCGCATCCC CTATTATGTT GGGCCTCTGG
     2281 CGAGAGGAAA CAGTAGGTTT GCTTGGATGA CCCGAAAGTC CGAAGAAACA ATCACTCCTT
     2341 GGAACTTCGA AGAAGTTGTC GATAAAGGGG CCAGCGCCCA GAGCTTTATC GAACGCATGA
     2401 CAAACTTTGA TAAGAATCTT CCAAATGAAA AGGTGCTTCC TAAACATAGT CTGCTTTATG
     2461 AGTATTTTAC GGTTTATAAC GAACTGACAA AGGTCAAATA TGTTACCGAA GGAATGCGCA
     2521 AACCAGCTTT TCTTAGCGGG GAACAAAAGA AAGCAATCGT TGATCTGCTC TTCAAAACAA
     2581 ATCGCAAAGT GACTGTTAAG CAGCTGAAAG AAGATTATTT CAAGAAGATA GAATGTTTTG
     2641 ATAGTGTTGA AATCAGCGGC GTTGAAGATA GATTCAATGC CAGCCTGGGG ACTTACCATG
     2701 ATCTGTTGAA AATCATCAAG GATAAAGACT TCCTGGATAA CGAAGAAAAt GAGGATATTC
     2761 TGGAaGATAT CGTTCTGACA CTGACTCTGT TTGAAGATCG TGAGATGATC GAGGAAAGAC
     2821 TTAAAACTTA TGCCCACCTC TTTGATGATA AGGTAATGAA ACAACTTAAA AGGCGCAGAT
     2881 ATACCGGGTG GGGCAGACTG TCCCGCAAAC TGATCAATGG AATCCGTGAT AAGCAGTCCG
     2941 GAAAGACAAT ACTGGATTTT CTGAAAAGCG ATGGGTTTGC AAATCGCAAT TTTATGCAAC
     3001 TAATTCATGA TGATAGTCTG ACATTCAAAG AAGACATCCA GAAAGCTCAG GTATCCGGCC
     3061 AGGGAGATAG TCTGCATGAA CATATCGCTA ATCTGGCCGG GTCTCCCGCC ATCAAGAAAG
     3121 GAATCCTGCA AACCGTGAAA GTTGTTGATG AACTGGTCAA AGTGATGGGT CGACATAAGC
     3181 CAGAAAATAT TGTTATCGAA ATGGCTAGAG AAAACCAAAC AACCCAGAAG GGACAAAAGA
     3241 ACTCGCGCGA GAGGATGAAA CGCATTGAAG AAGGGATTAA AGAACTGGGC AGTCAAATCC
     3301 TTAAAGAGCA TCCCGTTGAA AATACCCAAC TGCAAAACGA AAAGCTCTAT CTCTATTATC
     3361 TCCAGAATGG CAGAGACATG TATGTAGACC AGGAACTGGA TATCAATAGG CTGAGTGATT
     3421 ATGATGTCgc cgctATCGTT CCACAGAGTT TCCTTAAAGA CGATAGCATA GACAATAAGG
     3481 TCCTGACGAG ATCCGATAAG AATAGAGGGA AATCGGATAA CGTTCCTAGT GAAGAAGTGG
     3541 TCAAGAAGAT GAAGAACTAT TGGAGACAGC TTTTGAACGC AAAGCTGATT ACCCAGAGGA
     3601 AGTTCGATAA TCTGACGAAA GCCGAAAGAG GCGGGCTGAG TGAACTTGAT AAAGCCGGGT
     3661 TTATTAAACG CCAGCTGGTT GAAACCCGCC AGATTACCAA GCATGTAGCT CAGATCCTGG
     3721 ATAGTCGCAT GAATACCAAA TACGATGAAA ATGATAAACT TATCCGCGAG GTTAAAGTAA
     3781 TCACTCTGAA ATCCAAACTG GTTTCCGACT TCCGCAAAGA TTTCCAGTTC TATAAAGTGA
     3841 GAGAGATCAA CAATTACCAT CATGCACATG ATGCGTATTT GAATGCAGTC GTTGGCACCG
     3901 CCCTGATCAA GAAGTATCCA AAACTTGAAT CGGAGTTTGT CTATGGGGAT TATAAAGTTT
     3961 ATGATGTTAG GAAAATGATC GCCAAGTCCG AGCAGGAAAT AGGAAAAGCT ACTGCTAAGT
     4021 ATTTCTTTTA CTCCAATATT ATGAACTTCT TCAAAACAGA AATCACACTT GCTAATGGCG
     4081 AGATCCGCAA ACGCCCCTTG ATTGAAACCA ATGGTGAAAC CGGCGAAATC GTCTGGGATA
     4141 AAGGTCGCGA TTTTGCAACA GTACGCAAAG TGCTGTCAAT GCCGCAGGTC AATATCGTCA
     4201 AGAAAACAGA AGTGCAAACA GGAGGCTTCT CAAAGGAGAG CATCCTGCCT AAACGGAACT
     4261 CGGACAAGCT TATCGCCAGG AAGAAAGACT GGGACCCAAA GAAATATGGA GGGTTTGATA
     4321 GTCCTACGGT GGCCTATAGC GTCCTTGTAG TTGCCAAGGT AGAGAAGGGT AAGTCGAAGA
     4381 AGCTGAAATC AGTTAAAGAG CTGCTTGGTA TTACAATCAT GGAAAGAAGT TCATTTGAAA
     4441 AGAATCCGAT CGACTTTCTG GAAGCCAAAG GCTATAAGGA AGTTAAGAAA GACCTGATTA
     4501 TCAAACTTCC CAAATATAGT CTTTTCGAGC TGGAAAACGG GAGGAAACGA ATGCTAGCCA
     4561 GTGCAGGCGA ACTGCAGAAA GGCAATGAGC TAGCCCTACC ATCTAAGTAT GTAAACTTCC
     4621 TGTATCTGGC CAGTCATTAT GAAAAGCTGA AGGGGAGTCC AGAAGATAAC GAACAGAAGC
     4681 AGCTGTTTGT AGAGCAACAT AAGCATTATC TGGATGAGAT CATCGAGCAG ATTAGTGAAT
     4741 TTTCCAAGAG GGTTATCCTG GCTGATGCAA ATCTGGATAA AGTTCTTAGT GCTTATAACA
     4801 AACATAGAGA CAAACCAATA AGGGAACAGG CTGAAAATAT CATCCATCTG TTTACGCTGA
     4861 CGAATCTTGG CGCCCCAGCC GCCTTCAAGT ATTTCGATAC AACAATCGAT AGGAAACGCT
     4921 ATACGTCCAC AAAAGAAGTT CTGGATGCAA CCCTTATTCA TCAGTCAATT ACCGGGCTTT
     4981 ATGAAACACG CATCGATCTG AGTCAACTTG GAGGGGACCC TGCTTCTGGC GGAACTTCAT
     5041 CTGATGGTGG CACgTCaGAC GGAGGGTCAa gCaacACaGG CGGTAGCTCt GAcGGaGGgA
     5101 GCtcagaagg cgaacctgcg CATGCACTGA AGTACAACCT GACTGAGTTC AAGAAGACCA
     5161 AATCAAACAT CAACAAGGCA AGCCTCATTG GGTATAGCAA GTTCAACTCA GAAGGCTACG
     5221 TGTACGACCC TGAGTTCACA GGACCAACCC TGACTGCAAG TGGAGCAAAC TCACGGATCA
     5281 AGATCAAGGA CGGATCTAAC ATTCGCAAGA TGAACTCAGA TGAAACGTTT CTGTACATGG
     5341 GCTTCGATTC ACAAGATGGA AAGAGGGTGA ACGAGATTGA GTTCCTGACT GAAAACCAGA
     5401 AGATCTTCGT GTGCGGAAAC TCAATCTCCG TGGAAGTTCT TGAAGCGATC ATCGACAAGA
     5461 TTGGAGGGAC CGGACCGAAG AAGAAACGGA AAGTGTAATC TAGATGATCa GAACGGTcag
     5521 cctgctacgt aaattccgCA TGCcactgat TTTctgcTat ctttcatAAT AAAggcagcc
     5581 tTGCCTGAAG TCGCAGGTTG ACTTcatttt taccgggacg atctcTCGaa gcGGATCCat
     5641 gaaagacccc acctgtaggt ttggcaagct agctgcagta acgccatttt gcaaggcatg
     5701 gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat gaaaatagct
     5761 aacgttgggc caaacaggat atctgcggtg agcagtttcg gccccggccc ggggccaaga
     5821 acagatggtc accgcagttt cggccccggc ccgaggccaa gaacagatgg tccccagata
     5881 tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc ccccaaggac
     5941 ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg cttctgttcg
     6001 cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg gcgcgccagt
     6061 cctccgattg actgagtcgc ccggATGCAT CTTCATCGGA CAGGTTAGAC CAGACTTATC
     6121 CGGATAAGTC TGGTCCCGCC ACCATGGtga gcaagggcga ggagctgttc accggggtgg
     6181 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     6241 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     6301 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     6361 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     6421 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     6481 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     6541 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     6601 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     6661 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     6721 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     6781 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     6841 gcatggacga gctgtacaag taactagtac gtgctacgag atttcgattc caccgccgcc
     6901 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6961 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     7021 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7081 tctagttgtg gtttgtcccg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7141 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7201 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7261 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7321 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7381 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7441 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7501 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7561 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7621 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7681 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7741 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7801 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7861 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7921 ctgataaatc tggagccggt gagcgtggaa gccgcggtat cattgcagca ctggggccag
     7981 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8041 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8101 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8161 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8221 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8281 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8341 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8401 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8461 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8521 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8581 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8641 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8701 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8761 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8821 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc ttt
//
