LOCUS       KX638424                 711 bp    DNA     linear   SYN 03-MAR-2017
DEFINITION  Synthetic construct LSSmCherry gene, complete cds.
ACCESSION   KX638424
VERSION     KX638424.1
KEYWORDS    .
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Shen,Y., Chen,Y., Wu,J., Shaner,N.C. and Campbell,R.E.
  TITLE     Engineering of mCherry variants with long Stokes shift, red-shifted
            fluorescence, and low cytotoxicity
  JOURNAL   PLoS ONE 12 (2), E0171257 (2017)
   PUBMED   28241009
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  AUTHORS   Shen,Y., Shaner,N.C. and Campbell,R.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2016) Chemistry, University of Alberta, 11227
            Saskatchewan Drive, Edmonton, Alberta T6G2G2, Canada
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /db_xref="taxon:32630"
     CDS             1..711
                     /note="long stokes-shift variant of mCherry"
                     /codon_start=1
                     /transl_table=11
                     /product="LSSmCherry"
                     /protein_id="AQV08518.1"
                     /translation="MVSKGEEDNMTIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGLEASTERMYPEDG
                     ALKGESKERLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQ
                     YERSEGRHSTGGMDELYK"
ORIGIN      
        1 atggtgagca agggcgagga ggataacatg accatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa ttgggagcgc
      301 gtgatgaact ttgaggacgg cggtgtggtg accgtgaccc aggactcctc cctgcaggac
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctctga cggccccgta
      421 atgcagtgtc gtaccatggg cttggaggcc agtactgagc ggatgtaccc cgaggacggc
      481 gccctgaagg gcgagagtaa ggagaggctg aagctgaagg acggcggcca ctacgacgct
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      601 gacatcaagt tggacatcct ttcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgctccgagg gccgccactc caccggcggc atggacgagc tgtacaagta a
//

