LOCUS       pUC19_SOX2_T2A_2        6324 bp ds-DNA     circular     07-APR-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     2380..2397
                     /locus_tag="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5000..5593
                     /locus_tag="Puro"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6101..6121)
                     /locus_tag="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3506..4928
                     /locus_tag="TdTomato"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(165..847)
                     /locus_tag="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3446..3469
                     /locus_tag="NLS"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(2241..2309)
                     /locus_tag="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3476..3499
                     /locus_tag="NLS(1)"
                     /ApEinfo_label="NLS"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(6127..6149)
                     /locus_tag="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(945..1604)
                     /locus_tag="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5609..6086
                     /locus_tag="SOX2 3' homology arm"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3377..3439
                     /locus_tag="T2A"
                     /ApEinfo_fwdcolor="#f4160b"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4946..4999
                     /locus_tag="F2A"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2420..3370
                     /locus_tag="hSOX2"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1921 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     1981 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2041 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2101 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     2161 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     2221 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     2281 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     2341 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     2401 ttggaTCcac agcgcccgca tgtacaacat gatggagacg gagctgaagc cgccgggccc
     2461 gcagcaaact tcggggggcg gcggcggcaa ctccaccgcg gcggcggccg gcggcaacca
     2521 gaaaaacagc ccggaccgcg tcaagcggcc catgaatgcc ttcatggtgt ggtcccgcgg
     2581 gcagcggcgc aagatggccc aggagaaccc caagatgcac aactcggaga tcagcaagcg
     2641 cctgggcgcc gagtggaaac ttttgtcgga gacggagaag cggccgttca tcgacgaggc
     2701 taagcggctg cgagcgctgc acatgaagga gcacccggat tataaatacc ggccccggcg
     2761 gaaaaccaag acgctcatga agaaggataa gtacacgctg cccggcgggc tgctggcccc
     2821 cggcggcaat agcatggcga gcggggtcgg ggtgggcgcc ggcctgggcg cgggcgtgaa
     2881 ccagcgcatg gacagttacg cgcacatgaa cggctggagc aacggcagct acagcatgat
     2941 gcaggaccag ctgggctacc cgcagcaccc gggcctcaat gcgcacggcg cagcgcagat
     3001 gcagcccatg caccgctacg acgtgagcgc cctgcagtac aactccatga ccagctcgca
     3061 gacctacatg aacggctcgc ccacctacag catgtcctac tcgcagcagg gcacccctgg
     3121 catggctctt ggctccatgg gttcggtggt caagtccgag gccagctcca gcccccctgt
     3181 ggttacctct tcctcccact ccagggcgcc ctgccaggcc ggggacctcc gggacatgat
     3241 cagcatgtat ctccccggcg ccgaggtgcc ggaacccgcc gcccccagca gacttcacat
     3301 gtcccagcac taccagagcg gcccggtgcc cggcacggcc attaacggca cactgcccct
     3361 ctcacacatg GTCGACggct ccggagaggg cagaggaagt ctgctaacat gcggtgacgt
     3421 cgaggagaat cctggcccaA TCGATGGTcc aaaaaagaag agaaaggtaT TCGATGGTcc
     3481 aaaaaagaag agaaaggtaT TCGATatggt gagcaagggc gaggaggtca tcaaagagtt
     3541 catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg
     3601 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     3661 cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc
     3721 gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt
     3781 caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc
     3841 ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc
     3901 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
     3961 cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg
     4021 ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg
     4081 ctactactac gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt
     4141 ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg gcaccggcag
     4201 caccggcagc ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa
     4261 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
     4321 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     4381 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
     4441 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
     4501 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
     4561 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     4621 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     4681 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     4741 cggcggccGc tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     4801 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     4861 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga
     4921 cgagctgtAC AAGGGATCCC AGCTGCTGAA TTTTGACCTT CTTAAGCTTG CGGGAGACGT
     4981 CGAGTCCAAC CCTGGGCCCA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA
     5041 CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA
     5101 CACCGTCGAT CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC
     5161 GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT
     5221 CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT
     5281 GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC
     5341 GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA
     5401 GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG
     5461 GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG
     5521 CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG
     5581 CAAGCCCGGT GCCGTCGACT AGGAATTCCA CCCACAGCAA ATGACAGCTG CAAAAGAGAA
     5641 CACCAATCCC ATCCACACTC ACGCAAAAAC CGCGATGCCG ACAAGAAAAC TTTTATGAGA
     5701 GAGATCCTGG ACTTCTTTTT GGGGGACTAT TTTTGTACAG AGAAAACCTG GGGAGGGTGG
     5761 GGAGGGCGGG GGAATGGACC TTGTATAGAT CTGGAGGAAA GAAAGCTACG AAAAACTTTT
     5821 TAAAAGTTCT AGTGGTACGG TAGGAGCTTT GCAGGAAGTT TGCAAAAGTC TTTACCAATA
     5881 ATATTTAGAG CTAGTCTCCA AGCGACGAAA AAAATGTTTT AATATTTGCA AGCAACTTTT
     5941 GTACAGTATT TATCGAGATA AACATGGCAA TCAAAATGTC CATTGTTTAT AAGCTGAGAA
     6001 TTTGCCAATA TTTTTCAAGG AGAGGCTTCT TGCTGAATTT TGATTCTGCA GCTGAAATTT
     6061 AGGACAGTTG CAAACGTGAA AAGAAGAAGc ttggcgtaat catggtcata gctgtttcct
     6121 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6181 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6241 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6301 agaggcggtt tgcgtattgg gcgc   
//
