LOCUS       pLentiCRISPRv2.1       15327 bp    DNA     circular SYN  9-JAN-2015
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 04, 2014 from SnapGene Viewer 2.4.0 to Vector
            NTI(R) format
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|716828654|
COMMENT     VNTDBDATE|722432312|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLentiCRISPRv2.1_EFS-DD-Cas9-P2A-Ven|
COMMENT     VNTAUTHORNAME|s'erif|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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FEATURES             Location/Qualifiers
     LTR             828..1016
                     /vntifkey="19"
                     /label=5'LTR
                     /note="5' LTR (R and U5 portions U3 was replaced by the CMV promoter)"
     misc_feature    1067..1204
                     /vntifkey="21"
                     /label=Psi
                     /note="Psi"
     misc_feature    1124..1127
                     /vntifkey="21"
                     /label=SD
                     /note="SD splice donor"
     misc_feature    1171..1535
                     /vntifkey="21"
                     /label=gag
                     /note="gag"
     misc_feature    1681..1922
                     /vntifkey="21"
                     /label=RRE
                     /note="RRE"
     misc_feature    2440..2557
                     /vntifkey="21"
                     /label=CPPT
                     /note="CPPT central polypurine tract"
     misc_binding    2858..4737
                     /vntifkey="20"
                     /label=binding\site
                     /note="Filler (digest with BsmBI to replace filler with sgRNA oligos)"
     misc_feature    4738..4819
                     /vntifkey="21"
                     /label=chimeric\RNA\backbone\1\(BsmBI)
                     /note="chimeric RNA backbone 1 (BsmBI)"
     promoter        4832..5087
                     /vntifkey="30"
                     /label=EFS-NS
                     /note="EFS-NS"
     misc_feature    5439..9542
                     /vntifkey="21"
                     /label=hSpCas9
                     /note="hSpCas9"
     misc_feature    9543..9590
                     /vntifkey="21"
                     /label=NLS
                     /note="NLS"
     misc_feature    9591..9614
                     /vntifkey="21"
                     /label=FLAG
                     /note="FLAG"
     primer_bind     9609..9615
                     /vntifkey="28"
                     /label=primer\binding\site
     CDS             9615..9683
                     /codon_start=1
                     /vntifkey="4"
                     /label=P2A
     misc_feature    9615..9615
                     /vntifkey="21"
                     /label=P2A
                     /note="P2A"
     CDS             9684..10403
                     /codon_start=1
                     /vntifkey="4"
                     /label=Venus
     enhancer        10419..11007
                     /vntifkey="9"
                     /label=WPRE
     attenuator      11078..11313
                     /vntifkey="0"
                     /label=3'LTR
     primer_bind     11323..11332
                     /vntifkey="28"
                     /label=R1_W3L_PmeI
     rep_origin      13762..13987
                     /vntifkey="33"
                     /label=ColE1
                     /note="ColE1"
     misc_feature    14199..15005
                     /vntifkey="21"
                     /label=AmpR
                     /note="AmpR"
     CDS             5106..5432
                     /vntifkey="4"
                     /label=N-DD
BASE COUNT     4084 a      3797 c      3810 g      3636 t 
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 
     2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg 
     2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag 
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta 
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc 
     2821 ttggctttat atatcttgtg gaaaggacga aacaccggag acggttgtaa atgagcacac 
     2881 aaaatacaca tgctaaaata ttatattcta tgacctttat aaaatcaacc aaaatcttct 
     2941 ttttaataac tttagtatca ataattagaa tttttatgtt cctttttgca aacttttaat 
     3001 aaaaatgagc aaaataaaaa aacgctagtt ttagtaactc gcgttgtttt cttcaccttt 
     3061 aataatagct actccaccac ttgttcctaa gcggtcagct cctgcttcaa tcattttttg 
     3121 agcatcttca aatgttctaa ctccaccagc tgctttaact aaagcattgt ctttaacaac 
     3181 tgacttcatt agtttaacat cttcaaatgt tgcacctgat tttgaaaatc ctgttgatgt 
     3241 tttaacaaat tctaatccag cttcaacagc tatttcacaa gctttcatga tttcttcttt 
     3301 tgttaataaa caattttcca taatacattt aacaacatgt gatccagctg ctttttttac 
     3361 agctttcatg tcttctaaaa ctaattcata atttttgtct tttaatgcac caatatttaa 
     3421 taccatatca atttctgttg caccatcttt aattgcttca gaaacttcga atgcttttgt 
     3481 agctgttgtg catgcaccta gaggaaaacc tacaacattt gttattccta catttgtgcc 
     3541 ttttaataat tctttacaat agcttgttca atatgaatta acacaaactg ttgcaaaatc 
     3601 aaattcaatt gcttcatcac ataattgttt aatttcagct ttcgtagcat cttgttttaa 
     3661 taatgtgtga tctatatatt tgtttagttt cattttttct cctatatatt catttttaat 
     3721 tttaattctt taataatttc gtctacttta actttagcgt tttgaacaga ttcaccaaca 
     3781 cctataaaat aaatttttag tttaggttca gttccacttg ggcgaacagc aaatcatgac 
     3841 ttatcttcta aataaaattt tagtaagtct tgtcctggca tattatacat tccatcgatg 
     3901 tagtcttcaa cattaacaac tttaagtcca gcaatttgag ttaagggtgt tgctctcaat 
     3961 gatttcatta atggttcaat ttttaatttc ttttcttctg gtttaaaatt caagtttaaa 
     4021 gtgaaagtgt aatatgcacc catttcttta aataaatctt ctaaatagtc tactaatgtt 
     4081 ttattttgtt ttttataaaa tcaagcagcc tctgctatta atatagaagc ttgtattcca 
     4141 tctttatctc tagctgagtc atcaattaca tatccataac tttcttcata agcaaaaaca 
     4201 aaatttaatc cgttatcttc ttctttagca atttctctac ccattcattt aaatccagtt 
     4261 aaagttttta caatattaac tccatatttt tcatgagcga ttctatcacc caaatcactt 
     4321 gttacaaaac ttgaatatag agccggattt tttggaatgc tatttaagcg ttttagattt 
     4381 gataattttc aatcaattaa aattggtcct gtttgatttc catctaatct tacaaaatga 
     4441 ccatcatgtt ttattgccat tccaaatctg tcagcatctg ggtcattcat aataataata 
     4501 tctgcatcat gtttaatacc atattcaagc ggtatttttc atgcaggatc aaattctgga 
     4561 tttggattta caacattttt aaatgtttca tcttcaaatg catgctcttc aacctcaata 
     4621 acgttatatc ctgattcacg taatattttt ggggtaaatt tagttcctgt tccattaact 
     4681 gcgctaaaaa taatttttaa atctttttta gcttcttgct cttttttgta cgtctctgtt 
     4741 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc 
     4801 accgagtcgg tgcttttttg aattcgctag ctaggtcttg aaaggagtgg gaattggctc 
     4861 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 
     4921 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 
     4981 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 
     5041 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagag 
     5101 ccaccatggg agtgcaggtg gaaaccatct ccccaggtga cgggcgcacc ttccccaagc 
     5161 gcggccagac ctgtgtggtg cactacaccg ggatgcttga agatggaaag aaagtcgatt 
     5221 cctcccggga cagaaacaag ccctttaagt ttatgctagg caagcaggag gtgatccgag 
     5281 gctgggaaga aggggttgcc cagatgagtg tgggtcagag agccaaactg actatatctc 
     5341 cagattatgc ctatggtgcc actgggcacc caggcatcat cccaccacat gccactctcg 
     5401 tcttcgatgt ggagcttcta aaaccggaac cggctagcat ggacaagaag tacagcatcg 
     5461 gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc 
     5521 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga 
     5581 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg 
     5641 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca 
     5701 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg 
     5761 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct 
     5821 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca 
     5881 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact 
     5941 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc 
     6001 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg 
     6061 ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc 
     6121 agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc 
     6181 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca 
     6241 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg 
     6301 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag 
     6361 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc 
     6421 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca 
     6481 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca 
     6541 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg 
     6601 aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg 
     6661 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag 
     6721 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca 
     6781 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa 
     6841 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg 
     6901 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc 
     6961 tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga 
     7021 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca 
     7081 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact 
     7141 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca 
     7201 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg 
     7261 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg 
     7321 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga 
     7381 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca 
     7441 acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct 
     7501 tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca 
     7561 tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg 
     7621 ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg 
     7681 tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc 
     7741 agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca 
     7801 tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga 
     7861 acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac 
     7921 tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga 
     7981 aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg 
     8041 acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga 
     8101 acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc 
     8161 tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca 
     8221 caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca 
     8281 agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga 
     8341 aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct 
     8401 acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt 
     8461 tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg 
     8521 aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga 
     8581 ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg 
     8641 aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga 
     8701 gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag 
     8761 agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc 
     8821 ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca 
     8881 aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca 
     8941 tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca 
     9001 aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa 
     9061 acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc 
     9121 tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct 
     9181 cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg 
     9241 agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg 
     9301 acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga 
     9361 atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg 
     9421 acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga 
     9481 tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg 
     9541 acaagcgacc tgccgccaca aagaaggctg gacaggctaa gaagaagaaa gattacaaag 
     9601 acgatgacga taagggatcc ggagctacta acttcagcct gctgaagcag gctggggacg 
     9661 tggaggagaa ccctggacct aggatggtga gcaagggcga ggagctgttc accggggtgg 
     9721 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 
     9781 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca 
     9841 agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg 
     9901 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 
     9961 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 
    10021 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 
    10081 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 
    10141 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg 
    10201 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 
    10261 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca 
    10321 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 
    10381 gcatggacga gctgtacaag taaggatcct aagtcgacaa tcaacctctg gattacaaaa 
    10441 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 
    10501 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 
    10561 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 
    10621 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 
    10681 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 
    10741 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 
    10801 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc 
    10861 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 
    10921 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 
    10981 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc 
    11041 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 
    11101 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 
    11161 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 
    11221 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 
    11281 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct 
    11341 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 
    11401 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 
    11461 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 
    11521 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 
    11581 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 
    11641 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 
    11701 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 
    11761 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 
    11821 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 
    11881 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 
    11941 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 
    12001 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 
    12061 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 
    12121 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 
    12181 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 
    12241 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 
    12301 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt 
    12361 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc 
    12421 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac 
    12481 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc 
    12541 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg 
    12601 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag 
    12661 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg 
    12721 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg 
    12781 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac 
    12841 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc 
    12901 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 
    12961 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct 
    13021 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 
    13081 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg 
    13141 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 
    13201 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 
    13261 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 
    13321 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 
    13381 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 
    13441 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 
    13501 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 
    13561 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 
    13621 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 
    13681 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 
    13741 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 
    13801 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 
    13861 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 
    13921 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 
    13981 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 
    14041 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 
    14101 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 
    14161 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 
    14221 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 
    14281 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 
    14341 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 
    14401 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 
    14461 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 
    14521 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 
    14581 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 
    14641 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 
    14701 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 
    14761 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 
    14821 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 
    14881 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 
    14941 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 
    15001 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 
    15061 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 
    15121 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 
    15181 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 
    15241 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 
    15301 acatttcccc gaaaagtgcc acctgac 
//
