LOCUS       pH4-DEST     10591 bp    DNA     circular UNA 18-OCT-2007
DEFINITION  Cloning vector pBR322, complete sequence.
KEYWORDS    .
SOURCE      Cloning vector pBR322 (unknown)
  ORGANISM  Cloning vector pBR322
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     1.  pBR322 beta-lactamase (bla, conferring ampicillin resistance)
            is transcribed in vitro and in vivo both from its natural promoter
            (P3) and from promoter P1, a pSC101 promoter artificially
            juxtaposed with the bla sequence during construction of the pBR322
            plasmid.  (Ref: Brosius et al, J Biol Chem 257, 9205-9210 (1982)).
            2.  Corrects sequence error in tet gene deriving from the original
            pSC101 sequence, present in GenBank entry (see accession J01749)
            and NEB sequences dated 6/6/02 and earlier, based on sequencing of
            pBR322 and pACYC184 at NEB in 2007 (Mathieu-Sheltry, V. and Cantin,
            E., unpublished).  Correction is C1134T, representing a Thr350Ile
            correction in the expressed protein.
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Cloning vector pBR322"
                     /mol_type="other DNA"
     primer          4..26
                     /label=PBR-nanoluc5
     -35_signal      10..15
                     /note="tet (P2) promoter (clockwise); TTGACA"
     -10_signal      33..38
                     /note="tet (P2) promoter (clockwise); TTTAAT; transcript
                     start 45"
     -10_signal      complement(44..49)
                     /note="P1 promoter (counter-clockwise); TAAACT"
     primer          complement(61..79)
                     /label=PBR-nanoluc4
     -35_signal      complement(64..75)
                     /note="P1 promoter -35 sequence region; transcript starts
                     36, 37 (complementary strand)"
     CDS             86..1276
                     /gene="tet"
                     /note="tetR (confers resistance to tetracycline)"
                     /codon_start=1
                     /product="tetracycline efflux protein, class C"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     QLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                     ATST"
     gene            86..1276
                     /gene="tet"
     RBS             1905..1909
                     /note="rop RBS"
     CDS             1915..2106
                     /gene="rop"
                     /exception="alternative start codon"
                     /codon_start=1
                     /transl_table=11
                     /product="ROP protein"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            1915..2106
                     /gene="rop"
     primer          2132..2150
                     /label=PtRepisertscrn-2
     primer          2169..2228
                     /label=Insert-F
     primer          complement(2191..2209)
                     /label=pBR322-R
     OriT            2210..2974
                     /label=OriT-pRL2948a
     primer          3092..3151
                     /label=Ptrnai-47
     primer          complement(3113..3133)
                     /label=Ptrnai-49
     promoter        3134..3788
                     /label=H4-prom
     primer          complement(3163..3183)
                     /label=H4prom-out
     primer          complement(3765..3824)
                     /label=Ptrnai-48
     primer          3789..3812
                     /label=Ptrnai-52
     primer          3789..3848
                     /label=Ptrnai-52.2
     misc_feature    3789..3822
                     /feature_type="sequenced region"
                     /label="sequenced region"
     misc_feature    3789..3812
                     /feature_type=Motif
                     /Match=aagtcaagtcgacggtatcgataa
                     /Found at strand=Positive
     misc_feature    complement(3823..3947)
                     /feature_type="att site"
                     /label=AttR1
     misc_feature    3823..3848
                     /feature_type=Motif
                     /Match=acaagtttgtacaaaaaagctgaacg
                     /Found at strand=Positive
     primer          4030..4051
                     /label=Seqtest10
     CDS             4056..4715
                     /label=cat
     primer          4685..4703
                     /label=Seqtest11
     CDS             5057..5362
                     /label=ccdB
     primer          5251..5270
                     /label=Ccdb-out
     misc_feature    5403..5527
                     /feature_type="att site"
                     /label=AttR2
     primer          5498..5557
                     /label=Ptrnai-19
     primer          complement(5503..5562)
                     /label=Ptrnai-20
     misc_feature    5528..5537
                     /feature_type="Uncertain seq"
                     /label="Sequence uncertain"
     terminator      5538..5756
                     /label="FcpA term"
     primer          5717..5776
                     /label=Ptrnai-5
     primer          complement(5732..5791)
                     /label=Ptrnai-4
     ShBle           5757..7174
                     /label=PtBle
     promoter        5814..6500
                     /label=FcpF
     primer          complement(6408..6427)
                     /label=Ptcmpx-r3
     misc_feature    6457..6480
                     /feature_type=Motif
                     /Match=TAACCAAGTCGACGGTATCGATAA
                     /Found at strand=Positive
                     /label=aagtcaagtcgacggtatcgataa
     CDS             6501..6875
                     /label=ShBle
     primer          6594..6613
                     /label=SB2
     primer          complement(6856..6872)
                     /label=3'-shble
     terminator      6958..7155
                     /label=FcpA-Term
     CEN             7181..7297
     primer          7266..7291
                     /label=CenCheck1
     Ars             7298..7685
     primer          complement(7383..7405)
                     /label=Seqtest-4
     HIS             7686..8559
     primer          7975..7994
                     /label=Q-his-1
     primer          complement(8134..8153)
                     /label=Q-his-2
     primer          complement(8564..8621)
                     /label=Insert-R
     misc_feature    8572..8576
                     /feature_type="sequenced region"
                     /label="Deleted PstI site id in seq"
     primer          8581..8599
                     /label=pBR322-F
     primer          complement(8656..8676)
                     /label=PtRepisertscrn-1
     misc_RNA        complement(8764..9316)
                     /note="RNAII transcript (complementary strand)"
     rep_origin      complement(8764..9352)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     -35_signal      9170..9175
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      9192..9197
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        9206..9313
                     /note="RNAI transcript"
     -10_signal      complement(9326..9331)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(9347..9352)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     primer          9392..9414
                     /label=Amp-Primer-R
     CDS             complement(9523..10383)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(9523..10383)
                     /gene="bla"
     primer          9572..9592
                     /label=Ptrnai-50
     primer          complement(9647..9666)
                     /label=Ptrnai-51
     sig_peptide     complement(10315..10383)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
     RBS             complement(10391..10395)
                     /note="bla RBS"
     -10_signal      complement(10427..10432)
                     /note="bla (P3) promoter (counter-clockwise); GAGACA;
                     transcript start 4188 (complementary strand)"
     -35_signal      complement(10448..10453)
                     /note="bla (P3) promoter (counter-clockwise); TTCAAA"
     primer          complement(10570..10589)
                     /label=Amp-Primer-F
ORIGIN
        1 TTCTCATGTT TGACAGCTTA TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA
       61 TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG
      121 CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC CGGGCCTCTT
      181 GCGGGATATC GTCCATTCCG ACAGCATCGC CAGTCACTAT GGCGTGCTGC TAGCGCTATA
      241 TGCGTTGATG CAATTTCTAT GCGCACCCGT TCTCGGAGCA CTGTCCGACC GCTTTGGCCG
      301 CCGCCCAGTC CTGCTCGCTT CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC
      361 CACACCCGTC CTGTGGATCC TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC
      421 AGGTGCGGTT GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC GGGCTCGCCA
      481 CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT GGGTATGGTG GCAGGCCCCG TGGCCGGGGG
      541 ACTGTTGGGC GCCATCTCCT TGCATGCACC ATTCCTTGCG GCGGCGGTGC TCAACGGCCT
      601 CAACCTACTA CTGGGCTGCT TCCTAATGCA GGAGTCGCAT AAGGGAGAGC GTCGACCGAT
      661 GCCCTTGAGA GCCTTCAACC CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT
      721 CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC CGGCAGCGCT
      781 CTGGGTCATT TTCGGCGAGG ACCGCTTTCG CTGGAGCGCG ACGATGATCG GCCTGTCGCT
      841 TGCGGTATTC GGAATCTTGC ACGCCCTCGC TCAAGCCTTC GTCACTGGTC CCGCCACCAA
      901 ACGTTTCGGC GAGAAGCAGG CCATTATCGC CGGCATGGCG GCCGACGCGC TGGGCTACGT
      961 CTTGCTGGCG TTCGCGACGC GAGGCTGGAT GGCCTTCCCC ATTATGATTC TTCTCGCTTC
     1021 CGGCGGCATC GGGATGCCCG CGTTGCAGGC CATGCTGTCC AGGCAGGTAG ATGACGACCA
     1081 TCAGGGACAG CTTCAAGGAT CGCTCGCGGC TCTTACCAGC CTAACTTCGA TCATTGGACC
     1141 GCTGATCGTC ACGGCGATTT ATGCCGCCTC GGCGAGCACA TGGAACGGGT TGGCATGGAT
     1201 TGTAGGCGCC GCCCTATACC TTGTCTGCCT CCCCGCGTTG CGTCGCGGTG CATGGAGCCG
     1261 GGCCACCTCG ACCTGAATGG AAGCCGGCGG CACCTCGCTA ACGGATTCAC CACTCCAAGA
     1321 ATTGGAGCCA ATCAATTCTT GCGGAGAACT GTGAATGCGC AAACCAACCC TTGGCAGAAC
     1381 ATATCCATCG CGTCCGCCAT CTCCAGCAGC CGCACGCGGC GCATCTCGGG CAGCGTTGGG
     1441 TCCTGGCCAC GGGTGCGCAT GATCGTGCTC CTGTCGTTGA GGACCCGGCT AGGCTGGCGG
     1501 GGTTGCCTTA CTGGTTAGCA GAATGAATCA CCGATACGCG AGCGAACGTG AAGCGACTGC
     1561 TGCTGCAAAA CGTCTGCGAC CTGAGCAACA ACATGAATGG TCTTCGGTTT CCGTGTTTCG
     1621 TAAAGTCTGG AAACGCGGAA GTCAGCGCCC TGCACCATTA TGTTCCGGAT CTGCATCGCA
     1681 GGATGCTGCT GGCTACCCTG TGGAACACCT ACATCTGTAT TAACGAAGCG CTGGCATTGA
     1741 CCCTGAGTGA TTTTTCTCTG GTCCCGCCGC ATCCATACCG CCAGTTGTTT ACCCTCACAA
     1801 CGTTCCAGTA ACCGGGCATG TTCATCATCA GTAACCCGTA TCGTGAGCAT CCTCTCTCGT
     1861 TTCATCGGTA TCATTACCCC CATGAACAGA AATCCCCCTT ACACGGAGGC ATCAGTGACC
     1921 AAACAGGAAA AAACCGCCCT TAACATGGCC CGCTTTATCA GAAGCCAGAC ATTAACGCTT
     1981 CTGGAGAAAC TCAACGAGCT GGACGCGGAT GAACAGGCAG ACATCTGTGA ATCGCTTCAC
     2041 GACCACGCTG ATGAGCTTTA CCGCAGCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC
     2101 CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC
     2161 AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCGG ATCGTCTTGC
     2221 CTTGCTCGTC GGTGATGTAC TTACAGCTCG AAGTGCCTCT TCTTGATGGA GGCCATGGAA
     2281 CGTGCTTCCG AATCACGCGC ACCCCGATCC GTTACGTGAA AAAAGTCATG GCTCTGCCCT
     2341 CGGGCGGACC ACGCCCATCA TGACCTTGCC AAGCTCGTCC TGCTTCTCTT CGATCTTCGC
     2401 CAGCAGGGCG AGGATCGTGG CATCACCGAA CCGCGCCGTG CGCGGGTCGT CGGTGAGCCA
     2461 GAGTTTCAGC AGGCCGCCCA GGCGGCCCAG GTCGCCATTG ATGCGGGCCA GCTCGCGGAC
     2521 GTGCTCATAG TCCACGACGC CCGTGATTTT GTAGCCCTGG CCGACGGCCA GCAGGTAGGC
     2581 CGACAGGCTC ATGCCGGCCG CCGCCGCCTT TTCCTCAATC GCTCTTCGTT CGTCTGGAAG
     2641 GCAGTACACC TTGATAGGTG GGCTGCCCTT CCTGGTTGGC TTGGTTTCAT CAGCCATCCG
     2701 CTTGCCCTCA TCTGTTACGC CGGCGGTAGC CGGCCAGCCT CGCAGAGCAG GATTCCCGTT
     2761 GAGCACCGCC AGGTGCGAAT AAGGGACAGT GAAGAAGGAA CACCCGCTCG CGGGTGGGCC
     2821 TACTTCACCT ATCCTGCCCG GCTGACGCCG TTGGATACAC CAAGGAAAGT CTACACGAAC
     2881 CCTTTGGCAA AATCCTGTAT ATCGTGCGAA AAAGGATGGA TATACCGAAA AAATCGCTAT
     2941 AATGACCCCG AAGCAGGGTT ATGCAGCGGA AGATGCCATT CGCCATTCAG GCTGCGCAAC
     3001 TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA
     3061 TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA
     3121 ACGACGGCCA GTGGCAATCT CACGCACCAG GCGCTGGAAG GGCAACTTGC GGATGAGAAG
     3181 GTCCGTGGAC TTCTGGTAAC GACGGATCTC ACGCAGAGCG ACGGTTCCAG GGCGATAACG
     3241 GTGGGGCTTC TTGACTCCTC CGGTAGCCGG AGCGGACTTG CGGGCAGCCT TGGTGGCAAG
     3301 CTGCTTGCGC GGCGCTTTGC CTCCGGTGGA TTTACGGGCG GTTTGCTTGG TTCGGGCCAT
     3361 TTTGACGGTT TTTTTTACAA GAGAAGAGTT CTTGAAATTT GTGAGGTTAA AGTGTGTGGC
     3421 TTCCGCCGTA GTCAAGGAGC GTGCGGTTGC CGATCGCACC GGTACGTTCT GTAGAAATGA
     3481 ACACAGTGTG TTGAATTGAA AGTATGGCGC AGGTATGGTG TGTGATAAGT AGCAGCCGCG
     3541 CCGAGACAAA CAAACTTTGG TTTCTACGAC AATCTCTGTA GACAAGTACT AGAAACCCGT
     3601 TTGAACGAGC ATAAATCTGC ACCGGCAGGC CACCAGACAT CGTTTCAACG TAATATTCTA
     3661 CGTAACCATT TTATCCCAGG AAACCTACGG CCTGTGAACC ACCGAGACGG AGCACTCACA
     3721 ATTCGCTCTC GGCAACAACC GACAATCGTC TTACTCACAG TCAATACCGA AAACAAACAA
     3781 CAGCCAACaa gtcaagtcga cggtatcgat aagcttgata tcacaagttt gtacaaaaaa
     3841 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     3901 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag
     3961 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
     4021 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     4081 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     4141 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     4201 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     4261 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     4321 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     4381 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     4441 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     4501 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     4561 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     4621 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     4681 aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc ttactaaaag
     4741 ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata
     4801 tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt
     4861 tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta
     4921 agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat
     4981 caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag
     5041 aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     5101 tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc
     5161 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     5221 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
     5281 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     5341 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg
     5401 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     5461 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     5521 aagtggtgaa ttctgagcta cctcgacttt ggctgggaca ctttcagtga ggacaagaag
     5581 cttcagaagc gtgctatcga actcaaccag ggacgtgcgg cacaaatggg catccttgct
     5641 ctcatggtgc acgaacagtt gggagtctct atccttcctt aaaaatttaa ttttcattag
     5701 ttgcagtcac tccgctttgg tttcacagtc aggaataaca ctagctcgtc ttcaggACCA
     5761 TGATTACGCC AAGCTCGAAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG TACCTAACAG
     5821 GATTAGTGCA ATTCGAGTTG AATCACTGGG AAAAACATTG TCTTCTTTTT TATATTATCA
     5881 TTTGCATTAG TGCTGCAGTC GTAGATACTT GTTGGTTGAA AGACATCAGC TGGGAGGGAC
     5941 TGGACTAGCG TTTGGTAAGG AGACATACCT GTTAACGTTG GTTGCAAAAT TCCATTTCGC
     6001 GATTTATGTT ATCTGTAAAT CCTGATTTGT CTGGAATTCT TGATACTTCC GTTTTTTTAG
     6061 AGGCCAATGA TTAGCATCGG CGATTCTCAA AATAGCATTT TCGACATGCG GTGCTGATTT
     6121 CATAAACATA GACAACGCTT TTACATGTAA AAGTAACTTG CGGACTTGGA ACAGTGCTCT
     6181 GTTTTTGGTG TGAACGTAAC TCAGCAATAT TTCTGTGCTA GCAAGGTTTT TTATGATCGA
     6241 CCGAAGATCT CAAAACTCCG GGTCTTTCAA CTGTCTGACT AGACCATGTT CGTAACGTCG
     6301 GACAGCAGCT TTCGTTGTAC TGGTAGAATT TCTACGTGCG AAGCACGTGT AGGCAGGTTG
     6361 AACGACGATC CCTGCCGATG GATGGATTGG CACGCGGCGG AACGCTTTCG TGATCTACAC
     6421 CACCTGGATC TTCACATATC TTCGAAATCG AAAAATTAAC CAAGTCGACG GTATCGATAA
     6481 TATTCTAGCT GAGGGTACCC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG CTCACCGCGC
     6541 GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC CGGGACTTCG
     6601 TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC AGCGCGGTCC
     6661 AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC CTGGACGAGC
     6721 TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC GGGCCGGCCA
     6781 TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA
     6841 ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACCGAC GCCGACCAAC ACCGCCGGTC
     6901 CGACGCGGCC CGACGGGTCC GAGGCCTCGG AGATCTGGGC CCATGCGGCC GCAACAACTA
     6961 CCTCGACTTT GGCTGGGACA CTTTCAGTGA GGACAAGAAG CTTCAGAAGC GTGCTATCGA
     7021 ACTCAACCAG GGACGTGCGG CACAAATGGG CATCCTTGCT CTCATGGTGC ACGAACAGTT
     7081 GGGAGTCTCT ATCCTTCCTT AAAAATTTAA TTTTCATTAG TTGCAGTCAC TCCGCTTTGG
     7141 TTTCACAGTC AGGAATAACA CTAGCTCGTC TTCAgcgagc ATCACGTGCT ATAAAAATAA
     7201 TTATAATTTA AATTTTTTAA TATAAATATA TAAATTAAAA ATAGAAAGTA AAAAAAGAAA
     7261 TTAAAGAAAA AATAGTTTTT GTTTTCCGAA GATGTAAAAG ACTCTAGGGG GATCGCCAAC
     7321 AAATACTACC TTTTAcCTTG CTCTTCCTGC TCTCAGGTAT TAATGCCGAA TTGTTTCATC
     7381 TTGTCTGTGT AGAAGACCAC ACACGAAAAT CCTGTGATTT TACATTTTAC TTATCGTTAA
     7441 TCGAATGTAT ATCTATTTAA TCTGCTTTTC TTGTCTAATA AATATATATG TAAAGTACGC
     7501 TTTTTGTTGA AATTTTTTAA ACCTTTGTTT ATTTTTTTTT CTTCATTCCG TAACTCTTCT
     7561 ACCTTCTTTA TTTACTTTCT AAAATCCAAA TACAAAACAT AAAAATAAAT AAACACAGAG
     7621 TAAATTCCCA AATTATTCCA TCATTAAAAG ATACGAGGCG CGTGTAAGTT ACAGGCAAGC
     7681 GATCCTAGTA CACTCTATAT TTTTTTATGC CTCGGTAATG ATTTTCATTT TTTTTTTCCA
     7741 CCTAGCGGAT GACTCTTTTT TTTTCTTAGC GATTGGCATT ATCACATAAT GAATTATACA
     7801 TTATATAAAG TAATGTGATT TCTTCGAAGA ATATACTAAA AAATGAGCAG GCAAGATAAA
     7861 CGAAGGCAAA GATGACAGAG CAGAAAGCCC TAGTAAAGCG TATTACAAAT GAAACCAAGA
     7921 TTCAGATTGC GATCTCTTTA AAGGGTGGTC CCCTAGCGAT AGAGCACTCG ATCTTCCCAG
     7981 AAAAAGAGGC AGAAGCAGTA GCAGAACAGG CCACACAATC GCAAGTGATT AACGTCCACA
     8041 CAGGTATAGG GTTTCTGGAC CATATGATAC ATGCTCTGGC CAAGCATTCC GGCTGGTCGC
     8101 TAATCGTTGA GTGCATTGGT GACTTACACA TAGACGACCA TCACACCACT GAAGACTGCG
     8161 GGATTGCTCT CGGTCAAGCT TTTAAAGAGG CCCTAGGGGC CGTGCGTGGA GTAAAAAGGT
     8221 TTGGATCAGG ATTTGCGCCT TTGGATGAGG CACTTTCCAG AGCGGTGGTA GATCTTTCGA
     8281 ACAGGCCGTA CGCAGTTGTC GAACTTGGTT TGCAAAGGGA GAAAGTAGGA GATCTCTCTT
     8341 GCGAGATGAT CCCGCATTTT CTTGAAAGCT TTGCAGAGGC TAGCAGAATT ACCCTCCACG
     8401 TTGATTGTCT GCGAGGCAAG AATGATCATC ACCGTAGTGA GAGTGCGTTC AAGGCTCTTG
     8461 CGGTTGCCAT AAGAGAAGCC ACCTCGCCCA ATGGTACCAA CGATGTTCCC TCCACCAAAG
     8521 GTGTTCTTAT GTAGTTTTAC ACAGGAGTCT GGACTTGACC TCTAGAGTCG ACCTGCGCAT
     8581 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT
     8641 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
     8701 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC
     8761 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     8821 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     8881 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     8941 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     9001 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     9061 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     9121 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     9181 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     9241 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     9301 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
     9361 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
     9421 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
     9481 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG
     9541 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT
     9601 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
     9661 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC
     9721 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
     9781 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTGC
     9841 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
     9901 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
     9961 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
    10021 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
    10081 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAC
    10141 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
    10201 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
    10261 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
    10321 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
    10381 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG
    10441 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG
    10501 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG
    10561 GCGTATCACG AGGCCCTTTC GTCTTCAAGA A
//
