LOCUS       pNR-DEST     10902 bp    DNA     circular UNA 18-OCT-2007
DEFINITION  Cloning vector pBR322, complete sequence.
KEYWORDS    .
SOURCE      Cloning vector pBR322 (unknown)
  ORGANISM  Cloning vector pBR322
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     1.  pBR322 beta-lactamase (bla, conferring ampicillin resistance)
            is transcribed in vitro and in vivo both from its natural promoter
            (P3) and from promoter P1, a pSC101 promoter artificially
            juxtaposed with the bla sequence during construction of the pBR322
            plasmid.  (Ref: Brosius et al, J Biol Chem 257, 9205-9210 (1982)).
            2.  Corrects sequence error in tet gene deriving from the original
            pSC101 sequence, present in GenBank entry (see accession J01749)
            and NEB sequences dated 6/6/02 and earlier, based on sequencing of
            pBR322 and pACYC184 at NEB in 2007 (Mathieu-Sheltry, V. and Cantin,
            E., unpublished).  Correction is C1134T, representing a Thr350Ile
            correction in the expressed protein.
FEATURES             Location/Qualifiers
     source          1..10902
                     /organism="Cloning vector pBR322"
                     /mol_type="other DNA"
     primer          4..26
                     /label=PBR-nanoluc5
     -35_signal      10..15
                     /note="tet (P2) promoter (clockwise); TTGACA"
     -10_signal      33..38
                     /note="tet (P2) promoter (clockwise); TTTAAT; transcript
                     start 45"
     -10_signal      complement(44..49)
                     /note="P1 promoter (counter-clockwise); TAAACT"
     primer          complement(61..79)
                     /label=PBR-nanoluc4
     -35_signal      complement(64..75)
                     /note="P1 promoter -35 sequence region; transcript starts
                     36, 37 (complementary strand)"
     CDS             86..1276
                     /gene="tet"
                     /note="tetR (confers resistance to tetracycline)"
                     /codon_start=1
                     /product="tetracycline efflux protein, class C"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     QLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                     ATST"
     gene            86..1276
                     /gene="tet"
     RBS             1905..1909
                     /note="rop RBS"
     CDS             1915..2106
                     /gene="rop"
                     /exception="alternative start codon"
                     /codon_start=1
                     /transl_table=11
                     /product="ROP protein"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            1915..2106
                     /gene="rop"
     primer          2132..2150
                     /label=PtRepisertscrn-2
     primer          2169..2228
                     /label=Insert-F
     primer          complement(2191..2209)
                     /label=pBR322-R
     OriT            2210..2974
                     /label=OriT-pRL2948a
     primer          3094..3153
                     /label=Ptrnai-55
     primer          complement(3113..3133)
                     /label=Ptrnai-49
     promoter        3134..3819
                     /label=NR-prom
     primer          complement(3149..3169)
                     /label=NRprom-out
     primer          complement(3615..3632)
                     /label=NRout-2
     primer          complement(3801..3860)
                     /label=Ptrnai-56
     primer          3820..3843
                     /label=Ptrnai-52
     misc_feature    3820..3853
                     /feature_type="sequenced region"
                     /label="sequenced region"
     misc_feature    complement(3854..3978)
                     /feature_type="att site"
                     /label=AttR1
     primer          4061..4082
                     /label=Seqtest10
     CDS             4087..4746
                     /label=cat
     primer          4716..4734
                     /label=Seqtest11
     CDS             5088..5393
                     /label=ccdB
     primer          5282..5301
                     /label=Ccdb-out
     misc_feature    5434..5558
                     /feature_type="att site"
                     /label=AttR2
     primer          5539..5598
                     /label=Ptrnai-57
     primer          complement(5544..5603)
                     /label=Ptrnai-60
     misc_feature    5559..5568
                     /feature_type="Uncertain seq"
                     /label="Sequence uncertain"
     terminator      5569..6067
                     /label="NR term"
     primer          6028..6087
                     /label=Ptrnai-59
     primer          complement(6043..6102)
                     /label=Ptrnai-58
     ShBle           6068..7485
                     /label=PtBle
     promoter        6125..6811
                     /label=FcpF
     primer          complement(6719..6738)
                     /label=Ptcmpx-r3
     CDS             6812..7186
                     /label=ShBle
     primer          6905..6924
                     /label=SB2
     primer          complement(7167..7183)
                     /label=3'-shble
     terminator      7269..7466
                     /label=FcpA-Term
     CEN             7492..7608
     primer          7577..7602
                     /label=CenCheck1
     Ars             7609..7996
     primer          complement(7694..7716)
                     /label=Seqtest-4
     HIS             7997..8870
     primer          8286..8305
                     /label=Q-his-1
     primer          complement(8445..8464)
                     /label=Q-his-2
     primer          complement(8875..8932)
                     /label=Insert-R
     misc_feature    8883..8887
                     /feature_type="sequenced region"
                     /label="Deleted PstI site id in seq"
     primer          8892..8910
                     /label=pBR322-F
     primer          complement(8967..8987)
                     /label=PtRepisertscrn-1
     misc_RNA        complement(9075..9627)
                     /note="RNAII transcript (complementary strand)"
     rep_origin      complement(9075..9663)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     -35_signal      9481..9486
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      9503..9508
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        9517..9624
                     /note="RNAI transcript"
     -10_signal      complement(9637..9642)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(9658..9663)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     primer          9703..9725
                     /label=Amp-Primer-R
     CDS             complement(9834..10694)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(9834..10694)
                     /gene="bla"
     primer          9883..9903
                     /label=Ptrnai-50
     primer          complement(9958..9977)
                     /label=Ptrnai-51
     sig_peptide     complement(10626..10694)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
     RBS             complement(10702..10706)
                     /note="bla RBS"
     -10_signal      complement(10738..10743)
                     /note="bla (P3) promoter (counter-clockwise); GAGACA;
                     transcript start 4188 (complementary strand)"
     -35_signal      complement(10759..10764)
                     /note="bla (P3) promoter (counter-clockwise); TTCAAA"
     primer          complement(10881..10900)
                     /label=Amp-Primer-F
ORIGIN
        1 TTCTCATGTT TGACAGCTTA TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA
       61 TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG
      121 CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC CGGGCCTCTT
      181 GCGGGATATC GTCCATTCCG ACAGCATCGC CAGTCACTAT GGCGTGCTGC TAGCGCTATA
      241 TGCGTTGATG CAATTTCTAT GCGCACCCGT TCTCGGAGCA CTGTCCGACC GCTTTGGCCG
      301 CCGCCCAGTC CTGCTCGCTT CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC
      361 CACACCCGTC CTGTGGATCC TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC
      421 AGGTGCGGTT GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC GGGCTCGCCA
      481 CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT GGGTATGGTG GCAGGCCCCG TGGCCGGGGG
      541 ACTGTTGGGC GCCATCTCCT TGCATGCACC ATTCCTTGCG GCGGCGGTGC TCAACGGCCT
      601 CAACCTACTA CTGGGCTGCT TCCTAATGCA GGAGTCGCAT AAGGGAGAGC GTCGACCGAT
      661 GCCCTTGAGA GCCTTCAACC CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT
      721 CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC CGGCAGCGCT
      781 CTGGGTCATT TTCGGCGAGG ACCGCTTTCG CTGGAGCGCG ACGATGATCG GCCTGTCGCT
      841 TGCGGTATTC GGAATCTTGC ACGCCCTCGC TCAAGCCTTC GTCACTGGTC CCGCCACCAA
      901 ACGTTTCGGC GAGAAGCAGG CCATTATCGC CGGCATGGCG GCCGACGCGC TGGGCTACGT
      961 CTTGCTGGCG TTCGCGACGC GAGGCTGGAT GGCCTTCCCC ATTATGATTC TTCTCGCTTC
     1021 CGGCGGCATC GGGATGCCCG CGTTGCAGGC CATGCTGTCC AGGCAGGTAG ATGACGACCA
     1081 TCAGGGACAG CTTCAAGGAT CGCTCGCGGC TCTTACCAGC CTAACTTCGA TCATTGGACC
     1141 GCTGATCGTC ACGGCGATTT ATGCCGCCTC GGCGAGCACA TGGAACGGGT TGGCATGGAT
     1201 TGTAGGCGCC GCCCTATACC TTGTCTGCCT CCCCGCGTTG CGTCGCGGTG CATGGAGCCG
     1261 GGCCACCTCG ACCTGAATGG AAGCCGGCGG CACCTCGCTA ACGGATTCAC CACTCCAAGA
     1321 ATTGGAGCCA ATCAATTCTT GCGGAGAACT GTGAATGCGC AAACCAACCC TTGGCAGAAC
     1381 ATATCCATCG CGTCCGCCAT CTCCAGCAGC CGCACGCGGC GCATCTCGGG CAGCGTTGGG
     1441 TCCTGGCCAC GGGTGCGCAT GATCGTGCTC CTGTCGTTGA GGACCCGGCT AGGCTGGCGG
     1501 GGTTGCCTTA CTGGTTAGCA GAATGAATCA CCGATACGCG AGCGAACGTG AAGCGACTGC
     1561 TGCTGCAAAA CGTCTGCGAC CTGAGCAACA ACATGAATGG TCTTCGGTTT CCGTGTTTCG
     1621 TAAAGTCTGG AAACGCGGAA GTCAGCGCCC TGCACCATTA TGTTCCGGAT CTGCATCGCA
     1681 GGATGCTGCT GGCTACCCTG TGGAACACCT ACATCTGTAT TAACGAAGCG CTGGCATTGA
     1741 CCCTGAGTGA TTTTTCTCTG GTCCCGCCGC ATCCATACCG CCAGTTGTTT ACCCTCACAA
     1801 CGTTCCAGTA ACCGGGCATG TTCATCATCA GTAACCCGTA TCGTGAGCAT CCTCTCTCGT
     1861 TTCATCGGTA TCATTACCCC CATGAACAGA AATCCCCCTT ACACGGAGGC ATCAGTGACC
     1921 AAACAGGAAA AAACCGCCCT TAACATGGCC CGCTTTATCA GAAGCCAGAC ATTAACGCTT
     1981 CTGGAGAAAC TCAACGAGCT GGACGCGGAT GAACAGGCAG ACATCTGTGA ATCGCTTCAC
     2041 GACCACGCTG ATGAGCTTTA CCGCAGCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC
     2101 CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC
     2161 AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCGG ATCGTCTTGC
     2221 CTTGCTCGTC GGTGATGTAC TTACAGCTCG AAGTGCCTCT TCTTGATGGA GGCCATGGAA
     2281 CGTGCTTCCG AATCACGCGC ACCCCGATCC GTTACGTGAA AAAAGTCATG GCTCTGCCCT
     2341 CGGGCGGACC ACGCCCATCA TGACCTTGCC AAGCTCGTCC TGCTTCTCTT CGATCTTCGC
     2401 CAGCAGGGCG AGGATCGTGG CATCACCGAA CCGCGCCGTG CGCGGGTCGT CGGTGAGCCA
     2461 GAGTTTCAGC AGGCCGCCCA GGCGGCCCAG GTCGCCATTG ATGCGGGCCA GCTCGCGGAC
     2521 GTGCTCATAG TCCACGACGC CCGTGATTTT GTAGCCCTGG CCGACGGCCA GCAGGTAGGC
     2581 CGACAGGCTC ATGCCGGCCG CCGCCGCCTT TTCCTCAATC GCTCTTCGTT CGTCTGGAAG
     2641 GCAGTACACC TTGATAGGTG GGCTGCCCTT CCTGGTTGGC TTGGTTTCAT CAGCCATCCG
     2701 CTTGCCCTCA TCTGTTACGC CGGCGGTAGC CGGCCAGCCT CGCAGAGCAG GATTCCCGTT
     2761 GAGCACCGCC AGGTGCGAAT AAGGGACAGT GAAGAAGGAA CACCCGCTCG CGGGTGGGCC
     2821 TACTTCACCT ATCCTGCCCG GCTGACGCCG TTGGATACAC CAAGGAAAGT CTACACGAAC
     2881 CCTTTGGCAA AATCCTGTAT ATCGTGCGAA AAAGGATGGA TATACCGAAA AAATCGCTAT
     2941 AATGACCCCG AAGCAGGGTT ATGCAGCGGA AGATGCCATT CGCCATTCAG GCTGCGCAAC
     3001 TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA
     3061 TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA
     3121 ACGACGGCCA GTGAGCGAAC AGAAGACCCA ATCGTGGAGC AGTCATCATC ATTGTGGAAA
     3181 TGTGTCCTAG ACTGTTGCAG ACAGTCACCA ACGGAATGCT TTCGTTAAGA GCAAGGCGAC
     3241 AACGGTTCCT TCGTTGAGAT GGTGGTTCCA CTGTGCAGGT CACGTAGTGA AGTGATGTTG
     3301 CGGTAGTCTG CAATCCCGCT AGATACACGT CATTCGTGAA AGGCCTGTTG TTTTCATGTC
     3361 CGGGTCTGCA CGGGCACTAG AAGGAAGGAA GCAAAGACGA ACGGAAGTGA CTGTAAACGA
     3421 GAAGTGCACG AAGCCTTTTC TTGTGACGTC ACAACCCGAA TAGCCCTACG TGGCGTGCGA
     3481 CTTTCCGGCA CTTTGGATAT CGTTGCCCTA TATGTATTGG GTGTATACAT GTCGTATTCC
     3541 AACTACGACA GAAAACAGTC AGTTACGGGT ATTCGAACTT CCGGCACCTG CAGCGAGGAG
     3601 TTTTGTGTCC GTCGCACATC CTCCGTGCTT TCGGCACCTA CGTAGGCGGC AAACTTCCTG
     3661 CCTTCCGCCC CCGCCTTGCG GCATTCCGGC TCCGGGCCAG AATTGCCCGG GTGTTCACAA
     3721 TTTTGCCTCC TCACGAAAAA ACGTTCTACT TTGTATTTTG GTCGGGTTTC GGATCCTTCC
     3781 AGCAACCATT CTCATTCAAA GTCACCACTT GTGCGAACGa agtcaagtcg acggtatcga
     3841 taagcttgat atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
     3901 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     3961 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
     4021 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
     4081 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     4141 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     4201 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     4261 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     4321 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     4381 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     4441 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     4501 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     4561 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     4621 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
     4681 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     4741 gcgtaaacgc gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
     4801 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
     4861 atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
     4921 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
     4981 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
     5041 tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg
     5101 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
     5161 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
     5221 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
     5281 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
     5341 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
     5401 gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta
     5461 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
     5521 ttttacgttt ctcgttcagc tttcttgtac aaagtggtga attctgagAA GTTCTTGACT
     5581 GATTGTCATA TCAATTTTGC ACTTTACAAT ACATTTTCAA TAGCAATTTA CTTTAAGACT
     5641 AGCGCAATTT TTTTCTTACA TTAGATGAAC ATAAAACGAC GATGAGGATC ACTGTTAGGG
     5701 AAACGATGAA CTACGCTGAG GACTTCCTAT TATTCGTCCT TTGATGGCGT GGTTGTTCTT
     5761 TGGTTTGCGG TCGAGCGCAA CGACCAAGGC CTGTACCAAA AACGAAGTTG ATAATCAGAT
     5821 ATTTGAGTTG TAACAAGAAA CTAAAGCATT ATTTTAGCAG TAGCACGAAT CAGGATCTAA
     5881 AATGAACGCA ATTGCATGGT TTATGTCTAA GCTGCGTTAG AACTTAGAAA TTGAAGAGAA
     5941 CACGCCCTTC GAAGACGCCC GCCTTCGTCA GGAAGTCTTT CACAGCTTCA GTCATGCCTT
     6001 TCATACCACA AAGCAAAGCT CCTGAATTCC GAGGAATATC GACTCCGTCT TCCTCTAAAG
     6061 CAGTCTGACC ATGATTACGC CAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG
     6121 GTACCTAACA GGATTAGTGC AATTCGAGTT GAATCACTGG GAAAAACATT GTCTTCTTTT
     6181 TTATATTATC ATTTGCATTA GTGCTGCAGT CGTAGATACT TGTTGGTTGA AAGACATCAG
     6241 CTGGGAGGGA CTGGACTAGC GTTTGGTAAG GAGACATACC TGTTAACGTT GGTTGCAAAA
     6301 TTCCATTTCG CGATTTATGT TATCTGTAAA TCCTGATTTG TCTGGAATTC TTGATACTTC
     6361 CGTTTTTTTA GAGGCCAATG ATTAGCATCG GCGATTCTCA AAATAGCATT TTCGACATGC
     6421 GGTGCTGATT TCATAAACAT AGACAACGCT TTTACATGTA AAAGTAACTT GCGGACTTGG
     6481 AACAGTGCTC TGTTTTTGGT GTGAACGTAA CTCAGCAATA TTTCTGTGCT AGCAAGGTTT
     6541 TTTATGATCG ACCGAAGATC TCAAAACTCC GGGTCTTTCA ACTGTCTGAC TAGACCATGT
     6601 TCGTAACGTC GGACAGCAGC TTTCGTTGTA CTGGTAGAAT TTCTACGTGC GAAGCACGTG
     6661 TAGGCAGGTT GAACGACGAT CCCTGCCGAT GGATGGATTG GCACGCGGCG GAACGCTTTC
     6721 GTGATCTACA CCACCTGGAT CTTCACATAT CTTCGAAATC GAAAAATTAA CCAAGTCGAC
     6781 GGTATCGATA ATATTCTAGC TGAGGGTACC CATGGCCAAG TTGACCAGTG CCGTTCCGGT
     6841 GCTCACCGCG CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC
     6901 CCGGGACTTC GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT
     6961 CAGCGCGGTC CAGGACCAGG TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG
     7021 CCTGGACGAG CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC
     7081 CGGGCCGGCC ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA
     7141 CCCGGCCGGC AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACCGA CGCCGACCAA
     7201 CACCGCCGGT CCGACGCGGC CCGACGGGTC CGAGGCCTCG GAGATCTGGG CCCATGCGGC
     7261 CGCAACAACT ACCTCGACTT TGGCTGGGAC ACTTTCAGTG AGGACAAGAA GCTTCAGAAG
     7321 CGTGCTATCG AACTCAACCA GGGACGTGCG GCACAAATGG GCATCCTTGC TCTCATGGTG
     7381 CACGAACAGT TGGGAGTCTC TATCCTTCCT TAAAAATTTA ATTTTCATTA GTTGCAGTCA
     7441 CTCCGCTTTG GTTTCACAGT CAGGAATAAC ACTAGCTCGT CTTCAgcgag cATCACGTGC
     7501 TATAAAAATA ATTATAATTT AAATTTTTTA ATATAAATAT ATAAATTAAA AATAGAAAGT
     7561 AAAAAAAGAA ATTAAAGAAA AAATAGTTTT TGTTTTCCGA AGATGTAAAA GACTCTAGGG
     7621 GGATCGCCAA CAAATACTAC CTTTTAcCTT GCTCTTCCTG CTCTCAGGTA TTAATGCCGA
     7681 ATTGTTTCAT CTTGTCTGTG TAGAAGACCA CACACGAAAA TCCTGTGATT TTACATTTTA
     7741 CTTATCGTTA ATCGAATGTA TATCTATTTA ATCTGCTTTT CTTGTCTAAT AAATATATAT
     7801 GTAAAGTACG CTTTTTGTTG AAATTTTTTA AACCTTTGTT TATTTTTTTT TCTTCATTCC
     7861 GTAACTCTTC TACCTTCTTT ATTTACTTTC TAAAATCCAA ATACAAAACA TAAAAATAAA
     7921 TAAACACAGA GTAAATTCCC AAATTATTCC ATCATTAAAA GATACGAGGC GCGTGTAAGT
     7981 TACAGGCAAG CGATCCTAGT ACACTCTATA TTTTTTTATG CCTCGGTAAT GATTTTCATT
     8041 TTTTTTTTCC ACCTAGCGGA TGACTCTTTT TTTTTCTTAG CGATTGGCAT TATCACATAA
     8101 TGAATTATAC ATTATATAAA GTAATGTGAT TTCTTCGAAG AATATACTAA AAAATGAGCA
     8161 GGCAAGATAA ACGAAGGCAA AGATGACAGA GCAGAAAGCC CTAGTAAAGC GTATTACAAA
     8221 TGAAACCAAG ATTCAGATTG CGATCTCTTT AAAGGGTGGT CCCCTAGCGA TAGAGCACTC
     8281 GATCTTCCCA GAAAAAGAGG CAGAAGCAGT AGCAGAACAG GCCACACAAT CGCAAGTGAT
     8341 TAACGTCCAC ACAGGTATAG GGTTTCTGGA CCATATGATA CATGCTCTGG CCAAGCATTC
     8401 CGGCTGGTCG CTAATCGTTG AGTGCATTGG TGACTTACAC ATAGACGACC ATCACACCAC
     8461 TGAAGACTGC GGGATTGCTC TCGGTCAAGC TTTTAAAGAG GCCCTAGGGG CCGTGCGTGG
     8521 AGTAAAAAGG TTTGGATCAG GATTTGCGCC TTTGGATGAG GCACTTTCCA GAGCGGTGGT
     8581 AGATCTTTCG AACAGGCCGT ACGCAGTTGT CGAACTTGGT TTGCAAAGGG AGAAAGTAGG
     8641 AGATCTCTCT TGCGAGATGA TCCCGCATTT TCTTGAAAGC TTTGCAGAGG CTAGCAGAAT
     8701 TACCCTCCAC GTTGATTGTC TGCGAGGCAA GAATGATCAT CACCGTAGTG AGAGTGCGTT
     8761 CAAGGCTCTT GCGGTTGCCA TAAGAGAAGC CACCTCGCCC AATGGTACCA ACGATGTTCC
     8821 CTCCACCAAA GGTGTTCTTA TGTAGTTTTA CACAGGAGTC TGGACTTGAC CTCTAGAGTC
     8881 GACCTGCGCA TCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG
     8941 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT
     9001 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC
     9061 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
     9121 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA
     9181 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
     9241 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG
     9301 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC
     9361 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
     9421 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC
     9481 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG
     9541 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC
     9601 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT
     9661 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     9721 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     9781 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     9841 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     9901 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     9961 GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
    10021 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
    10081 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
    10141 GCCATTGCTG CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
    10201 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
    10261 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
    10321 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
    10381 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
    10441 CCGGCGTCAA CACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
    10501 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
    10561 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
    10621 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
    10681 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
    10741 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
    10801 ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC
    10861 TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTTCAAG AA
//
