LOCUS       pIB165                  4085 bp    DNA     circular      9-AUG-2007
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|452876576|
COMMENT     VNTDBDATE|454240896|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pIB165|
COMMENT     VNTAUTHORNAME|Indra Biswas|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT         #3=(COligo "P23-BamH1-R1" "ACACAGGATCCAACATCATTGTCATTCAT" "" 0 
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COMMENT        #7=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 4013 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 3972 1 4052 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "BsmFI" "gggac" 16 20 2 3972 1 4052 0 0 "") 
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COMMENT        #27=(CMSite "pJRS-F" "TAAGGCTATTGGTGTTTATGGC" "" 1 3679 0 100 100 100) 
COMMENT        #28=(CMSite "pJRS-R" "TACAATTGTCATCACCATTCTT" "" 1 19 1 100 100 100) 
COMMENT        #29=(CMSite "P23-BamH1-Apa1-F" "ACACAGGATCCGGGCCCCGAAAAGCCCTGACAACC" "" 
COMMENT             1 3711 0 68.5714 68.5714 68.5714) 
COMMENT        #30=(CMSite "P23-BamH1-R1" "ACACAGGATCCAACATCATTGTCATTCAT" "" 1 3889 1 
COMMENT             82.7586 82.7586 82.7586) 
COMMENT        #31=(CMSite "Bgl2-pASK-43-F2" 
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COMMENT                          19 "GGGACNNNNNNNNNNNNNNN" "CCCTGNNNNNNNNNNNNNNN") 
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FEATURES             Location/Qualifiers
     -10_signal      500..505
                     /vntifkey="47"
                     /label=-10
     -35_signal      477..482
                     /vntifkey="48"
                     /label=-35
     CDS             complement(2358..2516)
                     /vntifkey="4"
                     /label=REP\C
                     /note="RepC from pSH71 - regulates copy number"
     CDS             complement(1594..2289)
                     /vntifkey="4"
                     /label=REP\A
                     /note="RepA from pSH71 - regulates copy number"
     CDS             569..1216
                     /vntifkey="4"
                     /label=Cm(R)
                     /note="Chlorpamphenicol Acetyl-Transferase from pC194"
     promoter        3728..3906
                     /vntifkey="30"
                     /label=P23
BASE COUNT     1356 a       715 c       674 g      1340 t 
ORIGIN
        1 agcttaagtg attagtcaaa gaatggtgat gacaattgta caggttatca ataaagccat 
       61 tgatactctc attcttatcc cgtatttatt aacttctcct agtattcgta attatcatgg 
      121 ctgtcatggc gcattaacgg aataaagggt gtgcttaaat cgggccattt tgcgtaataa 
      181 gaaaaaggat taattatgag cgaattgaat taataataag gtaatagatt tacattagaa 
      241 aatgaaaggg gattttatgc gtgagaatgt tacagtctat cccggcaata gttaccctta 
      301 ttatcaagat aagaaagaaa aggatttttc gctacgctca aatcctttaa aaaaacacaa 
      361 aagaccacat tttttaatgt ggtctttatt cttcaactaa agcacccatt agttcaacaa 
      421 acgaaaattg gataaagtgg gatattttta aaatatatat ttatgttaca gtaatattga 
      481 cttttaaaaa aggattgatt ctaatgaaga aagcagacaa gtaagcctcc taaattcact 
      541 ttagataaaa atttaggagg catatcaaat gaactttaat aaaattgatt tagacaattg 
      601 gaagagaaaa gagatattta atcattattt gaaccaacaa acgactttta gtataaccac 
      661 agaaattgat attagtgttt tataccgaaa cataaaacaa gaaggatata aattttaccc 
      721 tgcatttatt ttcttagtga caagggtgat aaactcaaat acagctttta gaactggtta 
      781 caatagcgac ggagagttag gttattggga taagttagag ccactttata caatttttga 
      841 tggtgtatct aaaacattct ctggtatttg gactcctgta aagaatgact tcaaagagtt 
      901 ttatgattta tacctttctg atgtagagaa atataatggt tcggggaaat tgtttcccaa 
      961 aacacctata cctgaaaatg ctttttctct ttctattatt ccatggactt catttactgg 
     1021 gtttaactta aatatcaata ataatagtaa ttaccttcta cccattatta cagcaggaaa 
     1081 attcattaat aaaggtaatt caatatattt accgctatct ttacaggtac atcattctgt 
     1141 ttgtgatggt tatcatgcag gattgtttat gaactctatt caggaattgt cagataggcc 
     1201 taatgactgg cttttataat atgagataat gccgactgta ctttttacag tcggttttct 
     1261 aatgtcacta acctgccccg ttagttgaag aaggttttta tattacagct ccagatccat 
     1321 atccttcttt ttctgaaccg acttctcctt tttcgcttct ttattccaat tgctttattg 
     1381 acgttgagcc tcggaaccct taacaatccc aaaacttgtc gactttcgtc aggggggctt 
     1441 ttatttattc aataatccct cctctcaata aatctattgt tgtacttaat tcaacttcca 
     1501 tttctctgta tctttcaata cgctctttta agtccttaat ttcttttttt aattcctcat 
     1561 tttcagcaaa taactctttt tctttgtttg tcattttatt tcccccgttt cagcatcaag 
     1621 aacctttgca taacttgctc tatatccaca ctgataattg ccctcaaacc ataatctaaa 
     1681 ggcgctagag tttgttgaaa caatatcttt tacatcattc gtatttaaaa ttccaaactc 
     1741 cgctccccta aggcgaataa aagccattaa atcttttgta tttaccaaat tatagtcatc 
     1801 cactatatct aaaagtaaat tcttcaattc tcttttttgg ctttcatcaa gtgttatata 
     1861 gcggtcaata tcaaaatcat taatgttcaa aatatctttt ttgtcgtata tatgtttatt 
     1921 cttagcaata gcgtcctttg attcatgagt caaatattca tatgaacctt tgatataatc 
     1981 aagtatctca acatgagcaa ctgaactatt ccccaatttt cgcttaatct tgttcctaac 
     2041 gctttctatt gttacaggat ttcgtgcaat atatataacg tgatagtgtg gttttttata 
     2101 gtgctttcca tttcgtataa catcactact attccatgta tctttatctt ttttttcgtc 
     2161 catatcgtgt aaaggactga cagccataga tacgcccaaa ctctctaatt tttctttcca 
     2221 atcattagga attgagtcag gatataataa aaatccaaaa tttctagctt tagtattttt 
     2281 aatagccatg atatattacc ttatcaaaaa caagtagcga aaactcgtat ccttctaaaa 
     2341 acgcgagctt tcgcttattt tttttgttct gattcctttc ttgcatattc ttctatagct 
     2401 aacgccgcaa ccgcagattt tgaaaaacct ttttgtttcg ccatatctgt taatttttta 
     2461 tcttgctctt ttgtcagaga aatcataact ctttttttcg attctgaaat caccatttaa 
     2521 aaaactccaa tcaaataatt ttataaaatt agtgtatcac tttgtaatca taaaaacaac 
     2581 aataaagcta cttaaatata gatttataaa aaacgttggc gaaaacgttg gcgattcgtt 
     2641 ggcgattgaa aaacccctca aacccttgag ccagttggga tagagcgttt ttggcacaaa 
     2701 aattggcact cggcacttaa tggggggtcg tagtacggaa gcaaaattcg cttcctttcc 
     2761 ccccattttt ttccaaattc caaatttttt tcaaaaattt tccagcgcta ccgctcggca 
     2821 aaattgcaag caatttttaa aatcaaaccc atgagggaat ttcattccct caaactccct 
     2881 tgagcctcct ccaaccgaaa tagaaggacg ctgcgcttat tatttcattc agtcatcggc 
     2941 tttcataatc taacagacaa catcttcgct gcaaagcacg ctacgctcaa gggcttttac 
     3001 gctacgataa cgcctgtttt aacgattatg ccgataacta aacgaaataa acgctaaaac 
     3061 gtctcagaaa cgattttgag acgttttaat aaaaaatcga ccgaaaaata aatataaatc 
     3121 ggatatacaa tcggcaattg acgaaactgc aaaatatcct gtaaaggata cggattttat 
     3181 gaccgatgat gaagaaaaga atttgaaacg tttgtctgat ttggaggaag gtttacaccg 
     3241 taaaaggtta atctcctatg gtggtttgtt aaaagaaata cataaaaaat taaaccttga 
     3301 tgacacagaa gaaggcgatt tgattcatac agatgatgac gaaaaagccg atgaagatgg 
     3361 attttctatt attgcaatgt ggaattggga acggaaaaat tattttatta aagagtagtt 
     3421 caacaaacgg gccagtttgt tgaagattag atgctataat tgttattaaa aggattgaag 
     3481 gatgcttagg aagacgagtt attaatagct gaataagaac ggtgctctcc aaatattctt 
     3541 atttagaaaa cgaaatctaa aattatctga aaagggaatg agaatagtga atggaccaat 
     3601 aataatgact agagaagaaa gaatgaagat tgttcatgaa attaaggaac gaatattgga 
     3661 taaatatggg gatgatgtta aggctattgg tgtttatggc tctcttggtc gtcagactga 
     3721 tgggccccga aaagccctga caacccttgt tcctaaaaag gaataagcgt tcggtcagta 
     3781 aataatagaa ataaaaaatc agacctaaga ctgatgacaa aaagagaaaa ttttgataaa 
     3841 atagtcttag aattaaatta aaaagggagg ccaaatataa tgaaaaatat gaatgacaat 
     3901 gatgttggat ctctttaaga aggagatata caaatggcta gcagaggatc gcatcaccat 
     3961 caccatcacg gggccggaga ccgcggtccc gaattcgagc tcggtacccg gggatccctc 
     4021 gaggtcgacc tgcaggggga ccatggtctc agcgcttgga gccacccgca gttcgaaaaa 
     4081 taata 
//
