LOCUS       TK254_Addgene_#90229    9160 bp ds-DNA     circular     09-MAY-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    8551..8694
                     /label="lacZ_a"
     misc_feature    7884..7899
                     /label="cPPT"
     misc_feature    4023..4038
                     /label="cPPT"
     misc_feature    7884..7905
                     /label="U3PPT"
     misc_feature    4776..5021
                     /label="ERK KTR"
     CDS             132..992
                     /label="ORF frame 3"
     promoter        complement(8514..8532)
                     /label="T7_promoter"
     misc_feature    5028..5744
                     /label="mCerulean3"
     promoter        62..90
                     /label="AmpR_promoter"
     misc_feature    8297..8316
                     /label="EBV_rev_primer"
     rep_origin      8720..9026
                     /label="f1_origin"
     misc_feature    complement(6463..6486)
                     /label="MSCV_rev_primer"
     CDS             3168..4376
                     /label="ORF frame 3"
     misc_feature    6948..7763
                     /label="Hygromycin"
     CDS             5916..6545
                     /label="ORF frame 1"
     misc_feature    7954..8134
                     /label="HIV-1_5_LTR"
     misc_feature    3290..3523
                     /label="RRE"
     promoter        2174..2193
                     /label="T3_promoter"
     rep_origin      1147..1766
                     /label="pBR322_origin"
     misc_feature    2448..2628
                     /label="truncHIV-1_3_LTR"
     promoter        2075..2104
                     /label="lac_promoter"
     misc_feature    7954..8134
                     /label="truncHIV-1_3_LTR"
     promoter        2139..2157
                     /label="M13_reverse_primer"
     misc_feature    2736..2780
                     /label="HIV-1_psi_pack"
     misc_feature    8408..8427
                     /label="SV40pro_F_primer"
     misc_feature    5828..6403
                     /label="WPRE"
     misc_feature    2118..2140
                     /label="M13_pUC_rev_primer"
     misc_feature    complement(8551..8573)
                     /label="M13_pUC_fwd_primer"
     rep_origin      8346..8423
                     /label="SV40_origin"
     misc_feature    4179..4694
                     /label="hPGK"
     misc_feature    2448..2628
                     /label="HIV-1_5_LTR"
     terminator      8209..8328
                     /label="SV40_PA_terminator"
     gene            132..992
                     /label="Ampicillin"
     promoter        complement(8542..8558)
                     /label="M13_forward20_primer"
     misc_feature    6860..6879
                     /label="mPGK_F_primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc accggTCGTC GACTAGTCCA GTGTGGTGGA ATTCTGCAGA
     4741 TATCAACAAG TTTGTACAAA AAAGCAGGCT CCACCATGAA GGGCCGAAAG CCTCGGGACC
     4801 TGGAACTTCC ACTTAGCCCA AGCCTGCTGG GTGGCCAGGG ACCTGAACGG ACTCCAGGAT
     4861 CAGGAACAAG CTCTGGTCTT CAGGCACCGG GGCCAGCGCT TTCGCCCAGC AAGCGTAGCG
     4921 GTCTCGAGGA CCCAGCGACG CCATCGAAGA AGCCACGTAC CCCATCGGTG TCGTCAAGGC
     4981 TGGAGCGCCT GACCCTCCAG TCCAGCTTTC AATTCCCATC CACTAGTatg gtgagcaagg
     5041 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     5101 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     5161 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     5221 tgagctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     5281 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     5341 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     5401 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     5461 acgccatcca cggcaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
     5521 acttcggcct gaactgcaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     5581 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     5641 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     5701 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagTAAGCG GCCGCACTCG
     5761 AGATATCTAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCCAGCACAG TGGCGGCCGC
     5821 TCGAcaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5881 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5941 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6001 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6061 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6121 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6181 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     6241 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6301 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6361 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgGAA
     6421 TTCTACCGGG TAGGGGAGGC GCTTTTCCCA AGGCAGTCTG GAGCATGCGC TTTAGCAGCC
     6481 CCGCTGGGCA CTTGGCGCTA CACAAGTGGC CTCTGGCCTC GCACACATTC CACATCCACC
     6541 GGTAGGCGCC AACCGGCTCC GTTCTTTGGT GGCCCCTTCG CGCCACCTTC TACTCCTCCC
     6601 CTAGTCAGGA AGTTCCCCCC CGCCCCGCAG CTCGCGTCGT GCAGGACGTG ACAAATGGAA
     6661 GTAGCACGTC TCACTAGTCT CGTGCAGATG GACAGCACCG CTGAGCAATG GAAGCGGGTA
     6721 GGCCTTTGGG GCAGCGGCCA ATAGCAGCTT TGCTCCTTCG CTTTCTGGGC TCAGAGGCTG
     6781 GGAAGGGGTG GGTCCGGGGG CGGGCTCAGG GGCGGGCTCA GGGGCGGGGC GGGCGCCCGA
     6841 AGGTCCTCCG GAGGCCCGGC ATTCTGCACG CTTCAAAAGC GCACGTCTGC CGCGCTGTTC
     6901 TCCTCTTCCT CATCTCCGGG CCTTTCGACC TGCAGCCCAA GCTTACCATG AAAAAGCCTG
     6961 AACTCACCGC GACGTCTGTC GAGAAGTTTC TGATCGAAAA GTTCGACAGC GTCTCCGACC
     7021 TGATGCAGCT CTCGGAGGGC GAAGAATCTC GTGCTTTCAG CTTCGATGTA GGAGGGCGTG
     7081 GATATGTCCT GCGGGTAAAT AGCTGCGCCG ATGGTTTCTA CAAAGATCGT TATGTTTATC
     7141 GGCACTTTGC ATCGGCCGCG CTCCCGATTC CGGAAGTGCT TGACATTGGG GAATTCAGCG
     7201 AGAGCCTGAC CTATTGCATC TCCCGCCGTG CACAGGGTGT CACGTTGCAA GACCTGCCTG
     7261 AAACCGAACT GCCCGCTGTT CTGCAGCCGG TCGCGGAGGC CATGGATGCG ATCGCTGCGG
     7321 CCGATCTTAG CCAGACGAGC GGGTTCGGCC CATTCGGACC GCAAGGAATC GGTCAATACA
     7381 CTACATGGCG TGATTTCATA TGCGCGATTG CTGATCCCCA TGTGTATCAC TGGCAAACTG
     7441 TGATGGACGA CACCGTCAGT GCGTCCGTCG CGCAGGCTCT CGATGAGCTG ATGCTTTGGG
     7501 CCGAGGACTG CCCCGAAGTC CGGCACCTCG TGCACGCGGA TTTCGGCTCC AACAATGTCC
     7561 TGACGGACAA TGGCCGCATA ACAGCGGTCA TTGACTGGAG CGAGGCGATG TTCGGGGATT
     7621 CCCAATACGA GGTCGCCAAC ATCTTCTTCT GGAGGCCGTG GTTGGCTTGT ATGGAGCAGC
     7681 AGACGCGCTA CTTCGAGCGG AGGCATCCGG AGCTTGCAGG ATCGCCGCGG CTCCGGGGCG
     7741 TATATGCTCC GCATTGGTCT TGACGCCCGC CCCACGACCC GCAGCGCCCG ACCGAAAGGA
     7801 GCGCACGACC CCATGCATCT CGAGGGCCCG GTACctttaa gaccaatgac ttacaaggca
     7861 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc
     7921 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7981 gcctgggagc tctctggctg cctagggaac ccactgctta agcctcaata aagcttgcct
     8041 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8101 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     8161 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     8221 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8281 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     8341 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8401 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8461 gaggaggctt ttttggaggc ctaggctttt gcgtcgagac gtacccaatt cgccctatag
     8521 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     8581 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     8641 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     8701 cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     8761 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     8821 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     8881 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     8941 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     9001 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     9061 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     9121 taacgcgaat tttaacaaaa tattaacgtt tacaatttcc
//