LOCUS       TK151_Addgene_#90231    8740 bp ds-DNA     circular     09-MAY-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2448..2628
                     /label="HIV-1_5_LTR"
     misc_feature    2118..2140
                     /label="M13_pUC_rev_primer"
     promoter        complement(8122..8138)
                     /label="M13_forward20_primer"
     misc_feature    8131..8274
                     /label="lacZ_a"
     misc_feature    3290..3523
                     /label="RRE"
     rep_origin      8300..8606
                     /label="f1_origin"
     misc_feature    5825..6400
                     /label="WPRE"
     gene            132..992
                     /label="Ampicillin"
     CDS             5913..6542
                     /label="ORF frame 1"
     misc_feature    7877..7896
                     /label="EBV_rev_primer"
     misc_feature    6915..7313
                     /label="Blasticidin"
     terminator      7789..7908
                     /label="SV40_PA_terminator"
     rep_origin      7926..8003
                     /label="SV40_origin"
     CDS             3168..4376
                     /label="ORF frame 3"
     misc_feature    complement(8131..8153)
                     /label="M13_pUC_fwd_primer"
     promoter        2174..2193
                     /label="T3_promoter"
     promoter        62..90
                     /label="AmpR_promoter"
     misc_feature    2736..2780
                     /label="HIV-1_psi_pack"
     misc_feature    4776..5021
                     /label="ERK KTR"
     misc_feature    7464..7479
                     /label="cPPT"
     misc_feature    7534..7714
                     /label="HIV-1_5_LTR"
     misc_feature    2448..2628
                     /label="truncHIV-1_3_LTR"
     misc_feature    7988..8007
                     /label="SV40pro_F_primer"
     rep_origin      1147..1766
                     /label="pBR322_origin"
     promoter        2139..2157
                     /label="M13_reverse_primer"
     misc_feature    5028..5741
                     /label="mRuby2"
     misc_feature    4179..4694
                     /label="hPGK"
     misc_feature    7464..7485
                     /label="U3PPT"
     promoter        2075..2104
                     /label="lac_promoter"
     misc_feature    complement(6460..6483)
                     /label="MSCV_rev_primer"
     CDS             132..992
                     /label="ORF frame 3"
     misc_feature    7534..7714
                     /label="truncHIV-1_3_LTR"
     misc_feature    6857..6876
                     /label="mPGK_F_primer"
     promoter        complement(8094..8112)
                     /label="T7_promoter"
     misc_feature    4023..4038
                     /label="cPPT"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc accggTCGTC GACTAGTCCA GTGTGGTGGA ATTCTGCAGA
     4741 TATCAACAAG TTTGTACAAA AAAGCAGGCT CCACCATGAA GGGCCGAAAG CCTCGGGACC
     4801 TGGAACTTCC ACTTAGCCCA AGCCTGCTGG GTGGCCAGGG ACCTGAACGG ACTCCAGGAT
     4861 CAGGAACAAG CTCTGGTCTT CAGGCACCGG GGCCAGCGCT TTCGCCCAGC AAGCGTAGCG
     4921 GTCTCGAGGA CCCAGCGACG CCATCGAAGA AGCCACGTAC CCCATCGGTG TCGTCAAGGC
     4981 TGGAGCGCCT GACCCTCCAG TCCAGCTTTC AATTCCCATC CACTAGTATG GTGTCTAaGG
     5041 GCGAAGAGct gatcaaggaa aatatgcgta tgaaggtggt cAtggaaggt tcggtcaacg
     5101 gccaccaatt caaatgcaca ggtgaaggag aaggcaatcc gtacatggga actcaaacca
     5161 tgaggatcaa agtcatcgag ggaggacccc tgccatttgc ctttgacatt cttgccacgT
     5221 CGttcatgta tggcagccgt acttttatca agtacccgaa aggcattcct gatttcttta
     5281 aacagtcctt tcctgagggt tttacttggg aaagagttac Gagatacgaa gatggtggag
     5341 tcGtCaccgt Catgcaggac accagccttg aggatggctg tctcgtttac cacgTccaag
     5401 tcagAggggt aaactttccc tccaatggtC ccgtgatgca gaagaagacc aagggttggg
     5461 agCctaatac agagatgatg tatccagcag atggtggtct gaggggatac actcatatgg
     5521 cactgaaagt tgatggtggt ggccatCTGt ctTGctctTt Cgtaacaact tacaggtcaa
     5581 aaaagaccgt cgggaacatc aagatgcccg gtatccatgc cgttgatcac cgcctggaaa
     5641 ggttagagga aagtgacaat gaaatgttcg tagtacaacg cgaacacgca gttgccaagt
     5701 tcgccgggct tggtggtggg atggacgagc tgtacaagTA ATAAGCGGCC GCACTCGAGA
     5761 TATCTAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCC AGCACAGTGG CGGCCGCTCG
     5821 Acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     5881 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     5941 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6001 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6061 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6121 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6181 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     6241 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6301 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     6361 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgGAATTC
     6421 TACCGGGTAG GGGAGGCGCT TTTCCCAAGG CAGTCTGGAG CATGCGCTTT AGCAGCCCCG
     6481 CTGGGCACTT GGCGCTACAC AAGTGGCCTC TGGCCTCGCA CACATTCCAC ATCCACCGGT
     6541 AGGCGCCAAC CGGCTCCGTT CTTTGGTGGC CCCTTCGCGC CACCTTCTAC TCCTCCCCTA
     6601 GTCAGGAAGT TCCCCCCCGC CCCGCAGCTC GCGTCGTGCA GGACGTGACA AATGGAAGTA
     6661 GCACGTCTCA CTAGTCTCGT GCAGATGGAC AGCACCGCTG AGCAATGGAA GCGGGTAGGC
     6721 CTTTGGGGCA GCGGCCAATA GCAGCTTTGC TCCTTCGCTT TCTGGGCTCA GAGGCTGGGA
     6781 AGGGGTGGGT CCGGGGGCGG GCTCAGGGGC GGGCTCAGGG GCGGGGCGGG CGCCCGAAGG
     6841 TCCTCCGGAG GCCCGGCATT CTGCACGCTT CAAAAGCGCA CGTCTGCCGC GCTGTTCTCC
     6901 TCTTCCTCAT CTCCATGGCC AAGCCTTTGT CTCAAGAAGA ATCCACCCTC ATTGAAAGAG
     6961 CAACGGCTAC AATCAACAGC ATCCCCATCT CTGAAGACTA CAGCGTCGCC AGCGCAGCTC
     7021 TCTCTAGCGA CGGCCGCATC TTCACTGGTG TCAATGTATA TCATTTTACT GGGGGACCTT
     7081 GTGCAGAACT CGTGGTGCTG GGCACTGCTG CTGCTGCGGC AGCTGGCAAC CTGACTTGTA
     7141 TCGTCGCGAT CGGAAATGAG AACAGGGGCA TCTTGAGCCC CTGCGGACGG TGCCGACAGG
     7201 TGCTTCTCGA TCTGCATCCT GGGATCAAAG CCATAGTGAA GGACAGTGAT GGACAGCCGA
     7261 CGGCAGTTGG GATTCGTGAA TTGCTGCCCT CTGGTTATGT GTGGGAGGGC TAATGGTGCA
     7321 TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC GCAGCGCCCG ACCGAAAGGA
     7381 GCGCACGACC CCATGCATCT CGAGGGCCCG GTACctttaa gaccaatgac ttacaaggca
     7441 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc
     7501 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7561 gcctgggagc tctctggctg cctagggaac ccactgctta agcctcaata aagcttgcct
     7621 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7681 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7741 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     7801 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7861 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     7921 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7981 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8041 gaggaggctt ttttggaggc ctaggctttt gcgtcgagac gtacccaatt cgccctatag
     8101 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     8161 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     8221 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     8281 cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     8341 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     8401 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     8461 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     8521 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     8581 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     8641 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     8701 taacgcgaat tttaacaaaa tattaacgtt tacaatttcc
//