LOCUS       pKL517_Addgene_#90232   9097 bp ds-DNA     circular     09-MAY-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    7891..8071
                     /label="truncHIV-1_3_LTR"
     promoter        2174..2193
                     /label="T3_promoter"
     misc_feature    2448..2628
                     /label="HIV-1_5_LTR"
     misc_feature    complement(6400..6423)
                     /label="MSCV_rev_primer"
     promoter        2139..2157
                     /label="M13_reverse_primer"
     terminator      8146..8265
                     /label="SV40_PA_terminator"
     misc_feature    2448..2628
                     /label="truncHIV-1_3_LTR"
     misc_feature    4179..4694
                     /label="hPGK"
     promoter        complement(8451..8469)
                     /label="T7_promoter"
     misc_feature    6797..6816
                     /label="mPGK_F_primer"
     misc_feature    7821..7842
                     /label="U3PPT"
     misc_feature    complement(8488..8510)
                     /label="M13_pUC_fwd_primer"
     misc_feature    6885..7700
                     /label="Hygromycin"
     CDS             5853..6482
                     /label="ORF frame 1"
     CDS             132..992
                     /label="ORF frame 3"
     misc_feature    8488..8631
                     /label="lacZ_a"
     rep_origin      8283..8360
                     /label="SV40_origin"
     rep_origin      8657..8963
                     /label="f1_origin"
     CDS             3168..4376
                     /label="ORF frame 3"
     promoter        complement(8479..8495)
                     /label="M13_forward20_primer"
     misc_feature    8345..8364
                     /label="SV40pro_F_primer"
     rep_origin      1147..1766
                     /label="pBR322_origin"
     misc_feature    5765..6340
                     /label="WPRE"
     misc_feature    4965..5681
                     /label="mCerulean3"
     misc_feature    7891..8071
                     /label="HIV-1_5_LTR"
     misc_feature    2118..2140
                     /label="M13_pUC_rev_primer"
     gene            132..992
                     /label="Ampicillin"
     misc_feature    7821..7836
                     /label="cPPT"
     promoter        2075..2104
                     /label="lac_promoter"
     misc_feature    8234..8253
                     /label="EBV_rev_primer"
     misc_feature    4776..4958
                     /label="JNK KTR"
     misc_feature    3290..3523
                     /label="RRE"
     misc_feature    2736..2780
                     /label="HIV-1_psi_pack"
     promoter        62..90
                     /label="AmpR_promoter"
     misc_feature    4023..4038
                     /label="cPPT"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc accggTCGTC GACTAGTCCA GTGTGGTGGA ATTCTGCAGA
     4741 TATCAACAAG TTTGTACAAA AAAGCAGGCT CCACCATGAG TAACCCTAAG ATCCTAAAAC
     4801 AGAGCATGAC CTTGAACCTG GCCGACCCGG TGGGCAGTCT GAAGCCGCAC CTCCGCGCCA
     4861 AGCGCTCGGC CGGTCTCAAC GAAGACGAGT CGCCATCTAA GAAGCTGGCG TCGCCGGAGG
     4921 TGTCGTCAAG GCTGGAGCGC CTGACCCTCC AGTCCAGCAC TAGTatggtg agcaagggcg
     4981 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     5041 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     5101 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     5161 gctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     5221 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     5281 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     5341 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacg
     5401 ccatccacgg caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
     5461 tcggcctgaa ctgcaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     5521 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     5581 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     5641 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gTAAGCGGCC GCACTCGAGA
     5701 TATCTAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCC AGCACAGTGG CGGCCGCTCG
     5761 Acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     5821 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     5881 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     5941 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6001 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6061 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6121 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     6181 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6241 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     6301 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgGAATTC
     6361 TACCGGGTAG GGGAGGCGCT TTTCCCAAGG CAGTCTGGAG CATGCGCTTT AGCAGCCCCG
     6421 CTGGGCACTT GGCGCTACAC AAGTGGCCTC TGGCCTCGCA CACATTCCAC ATCCACCGGT
     6481 AGGCGCCAAC CGGCTCCGTT CTTTGGTGGC CCCTTCGCGC CACCTTCTAC TCCTCCCCTA
     6541 GTCAGGAAGT TCCCCCCCGC CCCGCAGCTC GCGTCGTGCA GGACGTGACA AATGGAAGTA
     6601 GCACGTCTCA CTAGTCTCGT GCAGATGGAC AGCACCGCTG AGCAATGGAA GCGGGTAGGC
     6661 CTTTGGGGCA GCGGCCAATA GCAGCTTTGC TCCTTCGCTT TCTGGGCTCA GAGGCTGGGA
     6721 AGGGGTGGGT CCGGGGGCGG GCTCAGGGGC GGGCTCAGGG GCGGGGCGGG CGCCCGAAGG
     6781 TCCTCCGGAG GCCCGGCATT CTGCACGCTT CAAAAGCGCA CGTCTGCCGC GCTGTTCTCC
     6841 TCTTCCTCAT CTCCGGGCCT TTCGACCTGC AGCCCAAGCT TACCATGAAA AAGCCTGAAC
     6901 TCACCGCGAC GTCTGTCGAG AAGTTTCTGA TCGAAAAGTT CGACAGCGTC TCCGACCTGA
     6961 TGCAGCTCTC GGAGGGCGAA GAATCTCGTG CTTTCAGCTT CGATGTAGGA GGGCGTGGAT
     7021 ATGTCCTGCG GGTAAATAGC TGCGCCGATG GTTTCTACAA AGATCGTTAT GTTTATCGGC
     7081 ACTTTGCATC GGCCGCGCTC CCGATTCCGG AAGTGCTTGA CATTGGGGAA TTCAGCGAGA
     7141 GCCTGACCTA TTGCATCTCC CGCCGTGCAC AGGGTGTCAC GTTGCAAGAC CTGCCTGAAA
     7201 CCGAACTGCC CGCTGTTCTG CAGCCGGTCG CGGAGGCCAT GGATGCGATC GCTGCGGCCG
     7261 ATCTTAGCCA GACGAGCGGG TTCGGCCCAT TCGGACCGCA AGGAATCGGT CAATACACTA
     7321 CATGGCGTGA TTTCATATGC GCGATTGCTG ATCCCCATGT GTATCACTGG CAAACTGTGA
     7381 TGGACGACAC CGTCAGTGCG TCCGTCGCGC AGGCTCTCGA TGAGCTGATG CTTTGGGCCG
     7441 AGGACTGCCC CGAAGTCCGG CACCTCGTGC ACGCGGATTT CGGCTCCAAC AATGTCCTGA
     7501 CGGACAATGG CCGCATAACA GCGGTCATTG ACTGGAGCGA GGCGATGTTC GGGGATTCCC
     7561 AATACGAGGT CGCCAACATC TTCTTCTGGA GGCCGTGGTT GGCTTGTATG GAGCAGCAGA
     7621 CGCGCTACTT CGAGCGGAGG CATCCGGAGC TTGCAGGATC GCCGCGGCTC CGGGGCGTAT
     7681 ATGCTCCGCA TTGGTCTTGA CGCCCGCCCC ACGACCCGCA GCGCCCGACC GAAAGGAGCG
     7741 CACGACCCCA TGCATCTCGA GGGCCCGGTA Cctttaagac caatgactta caaggcagct
     7801 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa
     7861 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     7921 tgggagctct ctggctgcct agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7981 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8041 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     8101 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     8161 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8221 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     8281 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8341 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     8401 gaggcttttt tggaggccta ggcttttgcg tcgagacgta cccaattcgc cctatagtga
     8461 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     8521 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     8581 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga
     8641 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     8701 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8761 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8821 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8881 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8941 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     9001 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     9061 cgcgaatttt aacaaaatat taacgtttac aatttcc
//