LOCUS pSR1404_pLenti_H2Bmceru 9919 bp ds-DNA circular 11-JUL-2017 DEFINITION . FEATURES Location/Qualifiers rep_origin complement(9467..9907) /label="F1 ori" promoter 4179..4694 /label="human PGK promoter" primer_bind complement(9301..9318) /label="M13-fwd" CDS 5301..6020 /label="mCerulean3" misc_feature 8579..8642 /label="hiv" CDS 7260..8075 /label="Hygro cds" misc_recomb 4746..4753 /label="AttR1" CDS 9389..9457 /label="LacZ alpha" CDS 330..989 /label="AmpR" CDS 5253..5288 /label="linker" misc_recomb 4754..4770 /label="AttL1" primer_bind 2137..2157 /label="M13-rev" rep_origin 9462..9917 /label="M13 origin" LTR 2448..2628 /label="HIV LTR 1" misc_feature 4023..4038 /label="cPPT" misc_feature 8643..8658 /label="cPPT" CDS 4869..5246 /label="H2B" misc_recomb complement(6054..6068) /label="AttR2" primer_bind 2175..2194 /label="T3" 3'UTR 6101..6676 /label="WPRE" rep_origin 1087..1769 /label="ColE1 origin" LTR 8713..8893 /label="HIV LTR 2" misc_feature 3290..3523 /label="RRE" misc_recomb complement(6044..6053) /label="AttL2" promoter 6734..7246 /label="mouse Pgk promoter" misc_binding 2109..2131 /label="LacO" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt 4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg 4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct 4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa 4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg 4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg 4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac 4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc 4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt 4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc 4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga 4681 cctctctccc cagggggatc accggTCGTC GACTAGTCCA GTGTGGTGGA ATTCTGCAGA 4741 TATCAACAAG TTTGTACAAA AAAGCAGGCT TTAAAGGAAC CAATTCAGTC GACTGGATCT 4801 CGAGCTCAAG CTTCGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGCCGG 4861 TCGCCACcat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc tccaagaagg 4921 cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc aaggagagct 4981 attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc atttcgtcca 5041 aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc gcaggtgagg 5101 cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag atccagacgg 5161 ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag ggtactaagg 5221 ccatcaccaa gtacaccagc gctaagaaca tggggcatgg caccggcagc accggcagcg 5281 gcagctcatc cggaactagt atggtgagca agggcgagga gctgttcacc ggggtggtgc 5341 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 5401 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 5461 tgcccgtgcc ctggcccacc ctcgtgacca ccctgagctg gggcgtgcag tgcttcgccc 5521 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 5581 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 5641 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 5701 acggcaacat cctggggcac aagctggagt acaacgccat ccacggcaac gtctatatca 5761 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcgg cctgaactgc aacatcgagg 5821 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 5881 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg 5941 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 6001 tggacgagct gtacaagtaa GCGGCCGCAC TCGAGATATC TAGACCCAGC TTTCTTGTAC 6061 AAAGTGGTTG ATATCCAGCA CAGTGGCGGC CGCTCGAcaa tcaacctctg gattacaaaa 6121 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 6181 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 6241 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 6301 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 6361 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 6421 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 6481 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc 6541 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 6601 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 6661 ggatctccct ttgggccgcc tccccgcctg gAATTCTGCA GATATCCGGA CTAGTGATCT 6721 CTCGAGGTTA ACGAATTCTA CCGGGTAGGG GAGGCGCTTT TCCCAAGGCA GTCTGGAGCA 6781 TGCGCTTTAG CAGCCCCGCT GGGCACTTGG CGCTACACAA GTGGCCTCTG GCCTCGCACA 6841 CATTCCACAT CCACCGGTAG GCGCCAACCG GCTCCGTTCT TTGGTGGCCC CTTCGCGCCA 6901 CCTTCTACTC CTCCCCTAGT CAGGAAGTTC CCCCCCGCCC CGCAGCTCGC GTCGTGCAGG 6961 ACGTGACAAA TGGAAGTAGC ACGTCTCACT AGTCTCGTGC AGATGGACAG CACCGCTGAG 7021 CAATGGAAGC GGGTAGGCCT TTGGGGCAGC GGCCAATAGC AGCTTTGCTC CTTCGCTTTC 7081 TGGGCTCAGA GGCTGGGAAG GGGTGGGTCC GGGGGCGGGC TCAGGGGCGG GCTCAGGGGC 7141 GGGGCGGGCG CCCGAAGGTC CTCCGGAGGC CCGGCATTCT GCACGCTTCA AAAGCGCACG 7201 TCTGCCGCGC TGTTCTCCTC TTCCTCATCT CCGGGCCTTT CGACCTGCAT CCCGCCACCA 7261 TGAAAAAGCC TGAACTCACC GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA 7321 GCGTCTCCGA CCTGATGCAG CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG 7381 TAGGAGGGCG TGGATATGTC CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC 7441 GTTATGTTTA TCGGCACTTT GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG 7501 GGGAATTCAG CGAGAGCCTG ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC 7561 AAGACCTGCC TGAAACCGAA CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG 7621 CGATCGCTGC GGCCGATCTT AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA 7681 TCGGTCAATA CACTACATGG CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC 7741 ACTGGCAAAC TGTGATGGAC GACACCGTCA GTGCGTCCGT CGCGCAGGCT CTCGATGAGC 7801 TGATGCTTTG GGCCGAGGAC TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT 7861 CCAACAATGT CCTGACGGAC AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA 7921 TGTTCGGGGA TTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT 7981 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC 8041 GGCTCCGGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC 8101 GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA 8161 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC 8221 TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG 8281 GAATAGAGTA GATGCCGACC GAACAAGAGC TGATTTCGAG AACGCCTCAG CCAGCAACTC 8341 GCGCGAGCCT AGCAAGGCAA ATGCGAGAGA ACGGCCTTAC GCTTGGTGGC ACAGTTCTCG 8401 TCCACAGTTC GCTAAGCTCG CTCGGCTGGG TCGCGGGAGG GCCGGTCGCA GTGATTCAGG 8461 CCCTTCTGGA TTGTGTTGGT CCCCAGGGCA CGATTGTCAT GCCCACGCAC TCGGGTGATC 8521 TGACTGATCC CGCAGATTGG AGATCGCCGC CCGTGCCTGC CGATTGGGTG CAGATCCGTC 8581 GAGGGCCCGG TACctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 8641 ttaaaagaaa aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt 8701 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctgc 8761 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 8821 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 8881 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 8941 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 9001 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 9061 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 9121 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 9181 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 9241 taggcttttg cgtcgagacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc 9301 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 9361 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 9421 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc 9481 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 9541 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 9601 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 9661 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 9721 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 9781 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 9841 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 9901 attaacgttt acaatttcc //