LOCUS       pSR1224_pLenti_PGK_H2B_ 9910 bp ds-DNA     circular     11-JUL-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3290..3523
                     /label="RRE"
     LTR             8704..8884
                     /label="HIV LTR 2"
     CDS             330..989
                     /label="AmpR"
     misc_recomb     4746..4753
                     /label="AttR1"
     misc_feature    8570..8633
                     /label="hiv"
     primer_bind     complement(9292..9309)
                     /label="M13-fwd"
     rep_origin      1087..1769
                     /label="ColE1 origin"
     CDS             4869..5246
                     /label="H2B"
     misc_recomb     complement(6035..6044)
                     /label="AttL2"
     primer_bind     2175..2194
                     /label="T3"
     3'UTR           6092..6667
                     /label="WPRE"
     promoter        6725..7237
                     /label="mouse Pgk promoter"
     promoter        4179..4694
                     /label="human PGK promoter"
     CDS             7251..8066
                     /label="Hygro cds"
     misc_feature    4023..4038
                     /label="cPPT"
     CDS             9380..9448
                     /label="LacZ alpha"
     primer_bind     2137..2157
                     /label="M13-rev"
     misc_recomb     4754..4770
                     /label="AttL1"
     CDS             5250..5285
                     /label="linker"
     CDS             5298..6008
                     /label="mRuby2"
     misc_recomb     complement(6045..6059)
                     /label="AttR2"
     rep_origin      9453..9908
                     /label="M13 origin"
     misc_feature    8634..8649
                     /label="cPPT"
     rep_origin      complement(9458..9898)
                     /label="F1 ori"
     misc_binding    2109..2131
                     /label="LacO"
     LTR             2448..2628
                     /label="HIV LTR 1"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc accggTCGTC GACTAGTCCA GTGTGGTGGA ATTCTGCAGA
     4741 TATCAACAAG TTTGTACAAA AAAGCAGGCT TTAAAGGAAC CAATTCAGTC GACTGGATCT
     4801 CGAGCTCAAG CTTCGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGCCGG
     4861 TCGCCACCat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc tccaagaagg
     4921 cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc aaggagagct
     4981 attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc atttcgtcca
     5041 aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc gcaggtgagg
     5101 cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag atccagacgg
     5161 ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag ggtactaagg
     5221 ccatcaccaa gtacaccagc gctaagATGg ggcatggcac cggcagcacc ggcagcggca
     5281 gctcatccgg aactagtATG GTGTCTAaGG GCGAAGAGct gatcaaggaa aatatgcgta
     5341 tgaaggtggt cAtggaaggt tcggtcaacg gccaccaatt caaatgcaca ggtgaaggag
     5401 aaggcaatcc gtacatggga actcaaacca tgaggatcaa agtcatcgag ggaggacccc
     5461 tgccatttgc ctttgacatt cttgccacgT CGttcatgta tggcagccgt acttttatca
     5521 agtacccgaa aggcattcct gatttcttta aacagtcctt tcctgagggt tttacttggg
     5581 aaagagttac Gagatacgaa gatggtggag tcGtCaccgt Catgcaggac accagccttg
     5641 aggatggctg tctcgtttac cacgTccaag tcagAggggt aaactttccc tccaatggtC
     5701 ccgtgatgca gaagaagacc aagggttggg agCctaatac agagatgatg tatccagcag
     5761 atggtggtct gaggggatac actcatatgg cactgaaagt tgatggtggt ggccatCTGt
     5821 ctTGctctTt Cgtaacaact tacaggtcaa aaaagaccgt cgggaacatc aagatgcccg
     5881 gtatccatgc cgttgatcac cgcctggaaa ggttagagga aagtgacaat gaaatgttcg
     5941 tagtacaacg cgaacacgca gttgccaagt tcgccgggct tggtggtggg atggacgagc
     6001 tgtacaagta aGCGGCCGCA CTCGAGATAT CTAGACCCAG CTTTCTTGTA CAAAGTGGTT
     6061 GATATCCAGC ACAGTGGCGG CCGCTCGAca atcaacctct ggattacaaa atttgtgaaa
     6121 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6181 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6241 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6301 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6361 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6421 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6481 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     6541 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     6601 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     6661 tttgggccgc ctccccgcct ggAATTCTGC AGATATCCGG ACTAGTGATC TCTCGAGGTT
     6721 AACGAATTCT ACCGGGTAGG GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA
     6781 GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCCACA
     6841 TCCACCGGTA GGCGCCAACC GGCTCCGTTC TTTGGTGGCC CCTTCGCGCC ACCTTCTACT
     6901 CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG GACGTGACAA
     6961 ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA GCACCGCTGA GCAATGGAAG
     7021 CGGGTAGGCC TTTGGGGCAG CGGCCAATAG CAGCTTTGCT CCTTCGCTTT CTGGGCTCAG
     7081 AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG CTCAGGGGCG GGCTCAGGGG CGGGGCGGGC
     7141 GCCCGAAGGT CCTCCGGAGG CCCGGCATTC TGCACGCTTC AAAAGCGCAC GTCTGCCGCG
     7201 CTGTTCTCCT CTTCCTCATC TCCGGGCCTT TCGACCTGCA TCCCGCCACC ATGAAAAAGC
     7261 CTGAACTCAC CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG
     7321 ACCTGATGCA GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC
     7381 GTGGATATGT CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT
     7441 ATCGGCACTT TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA
     7501 GCGAGAGCCT GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC
     7561 CTGAAACCGA ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG
     7621 CGGCCGATCT TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT
     7681 ACACTACATG GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA
     7741 CTGTGATGGA CGACACCGTC AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT
     7801 GGGCCGAGGA CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG
     7861 TCCTGACGGA CAATGGCCGC ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG
     7921 ATTCCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT TGTATGGAGC
     7981 AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG CGGCTCCGGG
     8041 GCGTATATGC TCCGCATTGG TCTTGACCAA CTCTATCAGA GCTTGGTTGA CGGCAATTTC
     8101 GATGATGCAG CTTGGGCGCA GGGTCGATGC GACGCAATCG TCCGATCCGG AGCCGGGACT
     8161 GTCGGGCGTA CACAAATCGC CCGCAGAAGC GCGGCCGTCT GGACCGATGG CTGTGTAGAA
     8221 GTACTCGCCG ATAGTGGAAA CCGACGCCCC AGCACTCGTC CGAGGGCAAA GGAATAGAGT
     8281 AGATGCCGAC CGAACAAGAG CTGATTTCGA GAACGCCTCA GCCAGCAACT CGCGCGAGCC
     8341 TAGCAAGGCA AATGCGAGAG AACGGCCTTA CGCTTGGTGG CACAGTTCTC GTCCACAGTT
     8401 CGCTAAGCTC GCTCGGCTGG GTCGCGGGAG GGCCGGTCGC AGTGATTCAG GCCCTTCTGG
     8461 ATTGTGTTGG TCCCCAGGGC ACGATTGTCA TGCCCACGCA CTCGGGTGAT CTGACTGATC
     8521 CCGCAGATTG GAGATCGCCG CCCGTGCCTG CCGATTGGGT GCAGATCCGT CGAGGGCCCG
     8581 GTACctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     8641 aaggggggac tggaagggct agctcactcc caacgaagac aagatctgct ttttgcttgt
     8701 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggctg cctagggaac
     8761 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8821 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8881 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     8941 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9001 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9061 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
     9121 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9181 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     9241 gcgtcgagac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     9301 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     9361 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     9421 acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc
     9481 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     9541 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     9601 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     9661 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     9721 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     9781 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     9841 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     9901 tacaatttcc
//