LOCUS TK066_Lenti_Addgene_#90 7912 bp ds-DNA circular 11-JUL-2017 DEFINITION . FEATURES Location/Qualifiers misc_feature complement(4752..4772) /label="Gateway Recomb2" misc_feature 3358..3735 /label="H2B" misc_feature 834..1014 /label="HIV LTR 1" misc_feature 3742..3777 /label="linker" misc_feature 2449..2464 /label="cPPT" CDS complement(6922..7581) /label="AmpR" misc_recomb complement(4752..4772) /label="attB2" rep_origin complement(6142..6824) /label="ColE1 origin" misc_feature 3103..3271 /label="human PGK promoter" misc_feature 2478..2499 /label="pSicoR shRNA sequencing oligo" misc_feature 3790..4725 /label="iRFP 670" misc_feature 2756..3101 /label="human PGK promoter" misc_feature 4803..5378 /label="WPRE" misc_feature 2634..2654 /label="Gateway recomb1" misc_feature 5581..5596 /label="cPPT" misc_recomb 2634..2654 /label="attB1" misc_feature 5918..6098 /label="HIV LTR 1" ORIGIN 1 TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTGCACT CTCAGTACAA TCTGCTCTGA 61 TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG 121 CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT 181 GCTTAGGGTT AGGCGTTTTG CGCTGCTTCG CGATGTACGG GCCAGATATA CGCGTTGACA 241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCGCTGAT CTTCAGACCT GGAGGAGGAG 1561 ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA AGTAGTAAAA ATTGAACCAT 1621 TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGTGCA GAGAGAAAAA AGAGCAGTGG 1681 GAATAGGAGC TTTGTTCCTT GGGTTCTTGG GAGCAGCAGG AAGCACTATG GGCGCAGCGT 1741 CAATGACGCT GACGGTACAG GCCAGACAAT TATTGTCTGG TATAGTGCAG CAGCAGAACA 1801 ATTTGCTGAG GGCTATTGAG GCGCAACAGC ATCTGTTGCA ACTCACAGTC TGGGGCATCA 1861 AGCAGCTCCA GGCAAGAATC CTGGCTGTGG AAAGATACCT AAAGGATCAA CAGCTCCTGG 1921 GGATTTGGGG TTGCTCTGGA AAACTCATTT GCACCACTGC TGTGCCTTGG AATGCTAGTT 1981 GGAGTAATAA ATCTCTGGAA CAGATTTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG 2041 AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG 2101 AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA 2161 ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG 2221 GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC 2281 CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG 2341 AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCGGCAC 2401 TGCGTGCGCC AATTCTGCAG ACAAATGGCA GTATTCATCC ACAATTTTAA AAGAAAAGGG 2461 GGGATTGGGG GGTACAGTGC AGGGGAAAGA ATAGTAGACA TAATAGCAAC AGACATACAA 2521 ACTAAAGAAT TACAAAAACA AATTACAAAA ATTCAAAATT TTCGGGTTTA TTACAGGGAC 2581 AGCAGAGATC CAGTTTGGTT AGTACCGGGC CGGTGGAATT CTGCAGATAT CAACAAGTTT 2641 GTACAAAAAA GCAGGCTTTA AAGGAACCAA TTCAGTCGAC TGGATCTCGA GCTCAAGCTT 2701 CGAATTCTGC AGTCGACGGT ACCGCGGGCC CGGGATCCAC CGGCCGGTCG CCACCccacg 2761 gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct 2821 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc 2881 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct 2941 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg 3001 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg 3061 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc 3121 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg 3181 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc 3241 gttgaccgaa tcaccgacct ctctccccag ggggatccac cggTCGTCGA CAGCTCAAGC 3301 TTCGAATTCT GCAGTCGACG GTACCGCGGG CCCGGGATCC ACCGGCCGGT CGCCACcatg 3361 ccagagccag cgaagtctgc tcccgccccg aaaaagggct ccaagaaggc ggtgactaag 3421 gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca aggagagcta ttccatctat 3481 gtgtacaagg ttctgaagca ggtccaccct gacaccggca tttcgtccaa ggccatgggc 3541 atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg caggtgaggc ttcccgcctg 3601 gcgcattaca acaagcgctc gaccatcacc tccagggaga tccagacggc cgtgcgcctg 3661 ctgctgcctg gggagttggc caagcacgcc gtgtccgagg gtactaaggc catcaccaag 3721 tacaccagcg ctaagaacat ggggcatggc accggcagca ccggcagcgg cagctcatcc 3781 ggaactagta tggcgcgtaa ggtcgatctc acctcctgcg atcgcgagcc gatccacatc 3841 cccggcagca ttcagccgtg cggctgcctg ctagcctgcg acgcgcaggc ggtgcggatc 3901 acgcgcatta cggaaaatgc cggcgcgttc tttggacgcg aaactccgcg ggtcggtgag 3961 ctactcgccg attacttcgg cgagaccgaa gcccatgcgc tgcgcaacgc actggcgcag 4021 tcctccgatc caaagcgacc ggcgctgatc ttcggttggc gcgacggcct gaccggccgc 4081 accttcgaca tctcactgca tcgccatgac ggtacatcga tcatcgagtt cgagcctgcg 4141 gcggccgaac aggccgacaa tccgctgcgg ctgacgcggc agatcatcgc gcgcaccaaa 4201 gaactgaagt cgctcgaaga gatggccgca cgggtgccgc gctatctgca ggcgatgctc 4261 ggctatcacc gcgtgatgtt gtaccgcttc gcggacgacg gctccgggat ggtgatcggc 4321 gaggcgaagc gcagcgacct cgagagcttt ctcggtcagc actttccggc gtcgctggtc 4381 ccgcagcagg cgcggctact gtacttgaag aacgcgatcc gcgtggtctc ggattcgcgc 4441 ggcatcagca gccggatcgt gcccgagcac gacgcctccg gcgccgcgct cgatctgtcg 4501 ttcgcgcacc tgcgcagcat ctcgccctgc catctcgaat ttctgcggaa catgggcgtc 4561 agcgcctcga tgtcgctgtc gatcatcatt gacggcacgc tatggggatt gatcatctgt 4621 catcattacg agccgcgtgc cgtgccgatg gcgcagcgcg tcgcggccga aatgttcgcc 4681 gacttcttat cgctgcactt caccgccgcc caccaccaac gctaaGCGGC CGCACTCGAG 4741 ATATCTAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC CAGCACAGTG GCGGCCCGAT 4801 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT 4861 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT 4921 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG 4981 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT 5041 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT 5101 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG 5161 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC 5221 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC 5281 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT 5341 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA TCGATACCGT 5401 CGACCTCGAT CGAGACCTAG AAAAACATGG AGCAATCACA AGTAGCAATA CAGCAGCTAC 5461 CAATGCTGAT TGTGCCTGGC TAGAAGCACA AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC 5521 ACCTCAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT 5581 AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATATCCTTGA 5641 TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG CAGAACTACA CACCAGGGCC 5701 AGGGATCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG CTAGTACCAG TTGAGCAAGA 5761 GAAGGTAGAA GAAGCCAATG AAGGAGAGAA CACCCGCTTG TTACACCCTG TGAGCCTGCA 5821 TGGGATGGAT GACCCGGAGA GAGAAGTATT AGAGTGGAGG TTTGACAGCC GCCTAGCATT 5881 TCATCACATG GCCCGAGAGC TGCATCCGGA CTGTACTGGG TCTCTCTGGT TAGACCAGAT 5941 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT 6001 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC 6061 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGC ATGTGAGCAA AAGGCCAGCA 6121 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 6181 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 6241 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 6301 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 6361 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 6421 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 6481 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 6541 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 6601 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 6661 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 6721 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 6781 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 6841 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 6901 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG 6961 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA 7021 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC 7081 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC 7141 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC 7201 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC 7261 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC 7321 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT 7381 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC 7441 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG 7501 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG 7561 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT 7621 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC 7681 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA 7741 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA 7801 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 7861 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG AC //