LOCUS       TK066_Lenti_Addgene_#90 7912 bp ds-DNA     circular     11-JUL-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(4752..4772)
                     /label="Gateway Recomb2"
     misc_feature    3358..3735
                     /label="H2B"
     misc_feature    834..1014
                     /label="HIV LTR 1"
     misc_feature    3742..3777
                     /label="linker"
     misc_feature    2449..2464
                     /label="cPPT"
     CDS             complement(6922..7581)
                     /label="AmpR"
     misc_recomb     complement(4752..4772)
                     /label="attB2"
     rep_origin      complement(6142..6824)
                     /label="ColE1 origin"
     misc_feature    3103..3271
                     /label="human PGK promoter"
     misc_feature    2478..2499
                     /label="pSicoR shRNA sequencing oligo"
     misc_feature    3790..4725
                     /label="iRFP 670"
     misc_feature    2756..3101
                     /label="human PGK promoter"
     misc_feature    4803..5378
                     /label="WPRE"
     misc_feature    2634..2654
                     /label="Gateway recomb1"
     misc_feature    5581..5596
                     /label="cPPT"
     misc_recomb     2634..2654
                     /label="attB1"
     misc_feature    5918..6098
                     /label="HIV LTR 1"
ORIGIN
        1 TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTGCACT CTCAGTACAA TCTGCTCTGA
       61 TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG
      121 CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT
      181 GCTTAGGGTT AGGCGTTTTG CGCTGCTTCG CGATGTACGG GCCAGATATA CGCGTTGACA
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCGCTGAT CTTCAGACCT GGAGGAGGAG
     1561 ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA AGTAGTAAAA ATTGAACCAT
     1621 TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGTGCA GAGAGAAAAA AGAGCAGTGG
     1681 GAATAGGAGC TTTGTTCCTT GGGTTCTTGG GAGCAGCAGG AAGCACTATG GGCGCAGCGT
     1741 CAATGACGCT GACGGTACAG GCCAGACAAT TATTGTCTGG TATAGTGCAG CAGCAGAACA
     1801 ATTTGCTGAG GGCTATTGAG GCGCAACAGC ATCTGTTGCA ACTCACAGTC TGGGGCATCA
     1861 AGCAGCTCCA GGCAAGAATC CTGGCTGTGG AAAGATACCT AAAGGATCAA CAGCTCCTGG
     1921 GGATTTGGGG TTGCTCTGGA AAACTCATTT GCACCACTGC TGTGCCTTGG AATGCTAGTT
     1981 GGAGTAATAA ATCTCTGGAA CAGATTTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG
     2041 AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG
     2101 AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA
     2161 ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG
     2221 GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC
     2281 CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG
     2341 AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCGGCAC
     2401 TGCGTGCGCC AATTCTGCAG ACAAATGGCA GTATTCATCC ACAATTTTAA AAGAAAAGGG
     2461 GGGATTGGGG GGTACAGTGC AGGGGAAAGA ATAGTAGACA TAATAGCAAC AGACATACAA
     2521 ACTAAAGAAT TACAAAAACA AATTACAAAA ATTCAAAATT TTCGGGTTTA TTACAGGGAC
     2581 AGCAGAGATC CAGTTTGGTT AGTACCGGGC CGGTGGAATT CTGCAGATAT CAACAAGTTT
     2641 GTACAAAAAA GCAGGCTTTA AAGGAACCAA TTCAGTCGAC TGGATCTCGA GCTCAAGCTT
     2701 CGAATTCTGC AGTCGACGGT ACCGCGGGCC CGGGATCCAC CGGCCGGTCG CCACCccacg
     2761 gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
     2821 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
     2881 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
     2941 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
     3001 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg
     3061 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
     3121 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
     3181 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
     3241 gttgaccgaa tcaccgacct ctctccccag ggggatccac cggTCGTCGA CAGCTCAAGC
     3301 TTCGAATTCT GCAGTCGACG GTACCGCGGG CCCGGGATCC ACCGGCCGGT CGCCACcatg
     3361 ccagagccag cgaagtctgc tcccgccccg aaaaagggct ccaagaaggc ggtgactaag
     3421 gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca aggagagcta ttccatctat
     3481 gtgtacaagg ttctgaagca ggtccaccct gacaccggca tttcgtccaa ggccatgggc
     3541 atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg caggtgaggc ttcccgcctg
     3601 gcgcattaca acaagcgctc gaccatcacc tccagggaga tccagacggc cgtgcgcctg
     3661 ctgctgcctg gggagttggc caagcacgcc gtgtccgagg gtactaaggc catcaccaag
     3721 tacaccagcg ctaagaacat ggggcatggc accggcagca ccggcagcgg cagctcatcc
     3781 ggaactagta tggcgcgtaa ggtcgatctc acctcctgcg atcgcgagcc gatccacatc
     3841 cccggcagca ttcagccgtg cggctgcctg ctagcctgcg acgcgcaggc ggtgcggatc
     3901 acgcgcatta cggaaaatgc cggcgcgttc tttggacgcg aaactccgcg ggtcggtgag
     3961 ctactcgccg attacttcgg cgagaccgaa gcccatgcgc tgcgcaacgc actggcgcag
     4021 tcctccgatc caaagcgacc ggcgctgatc ttcggttggc gcgacggcct gaccggccgc
     4081 accttcgaca tctcactgca tcgccatgac ggtacatcga tcatcgagtt cgagcctgcg
     4141 gcggccgaac aggccgacaa tccgctgcgg ctgacgcggc agatcatcgc gcgcaccaaa
     4201 gaactgaagt cgctcgaaga gatggccgca cgggtgccgc gctatctgca ggcgatgctc
     4261 ggctatcacc gcgtgatgtt gtaccgcttc gcggacgacg gctccgggat ggtgatcggc
     4321 gaggcgaagc gcagcgacct cgagagcttt ctcggtcagc actttccggc gtcgctggtc
     4381 ccgcagcagg cgcggctact gtacttgaag aacgcgatcc gcgtggtctc ggattcgcgc
     4441 ggcatcagca gccggatcgt gcccgagcac gacgcctccg gcgccgcgct cgatctgtcg
     4501 ttcgcgcacc tgcgcagcat ctcgccctgc catctcgaat ttctgcggaa catgggcgtc
     4561 agcgcctcga tgtcgctgtc gatcatcatt gacggcacgc tatggggatt gatcatctgt
     4621 catcattacg agccgcgtgc cgtgccgatg gcgcagcgcg tcgcggccga aatgttcgcc
     4681 gacttcttat cgctgcactt caccgccgcc caccaccaac gctaaGCGGC CGCACTCGAG
     4741 ATATCTAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC CAGCACAGTG GCGGCCCGAT
     4801 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT
     4861 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT
     4921 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG
     4981 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT
     5041 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT
     5101 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG
     5161 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC
     5221 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC
     5281 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT
     5341 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA TCGATACCGT
     5401 CGACCTCGAT CGAGACCTAG AAAAACATGG AGCAATCACA AGTAGCAATA CAGCAGCTAC
     5461 CAATGCTGAT TGTGCCTGGC TAGAAGCACA AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC
     5521 ACCTCAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT
     5581 AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATATCCTTGA
     5641 TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG CAGAACTACA CACCAGGGCC
     5701 AGGGATCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG CTAGTACCAG TTGAGCAAGA
     5761 GAAGGTAGAA GAAGCCAATG AAGGAGAGAA CACCCGCTTG TTACACCCTG TGAGCCTGCA
     5821 TGGGATGGAT GACCCGGAGA GAGAAGTATT AGAGTGGAGG TTTGACAGCC GCCTAGCATT
     5881 TCATCACATG GCCCGAGAGC TGCATCCGGA CTGTACTGGG TCTCTCTGGT TAGACCAGAT
     5941 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT
     6001 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC
     6061 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGC ATGTGAGCAA AAGGCCAGCA
     6121 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC
     6181 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     6241 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     6301 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     6361 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     6421 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     6481 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     6541 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     6601 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     6661 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     6721 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
     6781 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT
     6841 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA
     6901 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG
     6961 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA
     7021 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC
     7081 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC
     7141 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC
     7201 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC
     7261 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC
     7321 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT
     7381 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC
     7441 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG
     7501 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG
     7561 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT
     7621 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC
     7681 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA
     7741 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA
     7801 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA
     7861 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG AC
//